##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2962921
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249491633425258	33.0	31.0	34.0	28.0	34.0
2	31.379427598643368	33.0	31.0	34.0	28.0	34.0
3	31.456194073348563	34.0	31.0	34.0	28.0	34.0
4	34.93690449390989	37.0	35.0	37.0	32.0	37.0
5	34.66252795805221	37.0	35.0	37.0	32.0	37.0
6	34.635211671185296	37.0	35.0	37.0	32.0	37.0
7	34.50508636578566	37.0	35.0	37.0	31.0	37.0
8	34.4503518656083	37.0	35.0	37.0	31.0	37.0
9	36.02828121303268	39.0	37.0	39.0	31.0	39.0
10-11	35.91656088704356	39.0	37.0	39.0	31.0	39.0
12-13	35.83600997124122	39.0	37.0	39.0	30.0	39.0
14-15	37.06246875971381	40.0	38.0	41.0	31.0	41.0
16-17	37.022286284379504	40.0	38.0	41.0	31.0	41.0
18-19	36.974244166483004	40.0	37.5	41.0	30.5	41.0
20-21	36.912864197189194	40.0	37.0	41.0	30.0	41.0
22-23	36.817557572409115	40.0	37.0	41.0	30.0	41.0
24-25	36.73355516397501	40.0	37.0	41.0	30.0	41.0
26-27	36.60310787901534	40.0	37.0	41.0	30.0	41.0
28-29	36.47906390349253	40.0	37.0	41.0	30.0	41.0
30-31	36.32055056479737	40.0	36.5	41.0	29.0	41.0
32-33	36.26653478104883	40.0	36.5	41.0	29.5	41.0
34-35	36.39339337768371	40.0	37.0	41.0	30.0	41.0
36-37	36.38971508183985	40.0	37.0	41.0	30.0	41.0
38-39	36.27741779142947	40.0	37.0	41.0	29.0	41.0
40-41	36.193543972316505	40.0	36.0	41.0	28.5	41.0
42-43	36.067032499347775	40.0	36.0	41.0	28.0	41.0
44-45	35.93449724106718	40.0	36.0	41.0	27.5	41.0
46-47	35.781467680036016	40.0	35.5	41.0	27.0	41.0
48-49	35.63697445865077	40.0	35.0	41.0	27.0	41.0
50-51	35.47568818068386	39.5	35.0	41.0	26.0	41.0
52-53	35.29678955328205	39.0	35.0	41.0	26.0	41.0
54-55	35.07930906696466	39.0	35.0	41.0	26.0	41.0
56-57	34.850363037016514	39.0	35.0	41.0	25.0	41.0
58-59	34.6068349442999	38.0	34.5	40.0	24.5	41.0
60-61	34.3256289992207	38.0	34.0	40.0	23.5	41.0
62-63	34.01906024494072	37.0	34.0	40.0	23.0	41.0
64-65	33.67703678228343	37.0	34.0	40.0	22.0	41.0
66-67	33.32608378691164	36.0	34.0	39.0	22.0	41.0
68-69	32.96433958245933	36.0	33.0	39.0	21.5	41.0
70-71	32.562470615990094	35.0	33.0	38.5	20.0	40.0
72-73	32.195115225819386	35.0	33.0	37.5	19.0	40.0
74-75	31.785976912648024	35.0	32.5	37.0	17.5	39.0
76-77	29.07008624259641	33.0	28.5	34.5	9.5	36.5
78-79	30.639015856312064	34.5	31.0	36.0	10.5	37.0
80-81	30.81835813374707	35.0	32.0	35.5	10.5	37.0
82-83	30.638089574443597	35.0	32.0	35.0	7.0	37.0
84-85	30.43242968678544	35.0	32.0	35.0	6.0	36.0
86-87	30.215993440257098	35.0	31.5	35.0	2.0	36.0
88-89	29.93810381714531	34.5	31.0	35.0	2.0	36.0
90-91	29.729303110005297	34.0	31.0	35.0	2.0	35.0
92-93	29.562078435435843	34.0	31.0	35.0	2.0	35.0
94-95	29.444217378728627	34.0	31.0	35.0	2.0	35.0
96-97	29.227225768084942	34.0	31.0	35.0	2.0	35.0
98-99	29.008181284617443	34.0	31.0	35.0	2.0	35.0
100-101	27.85714722059751	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61798.0
3	20886.0
4	11334.0
5	7807.0
6	7070.0
7	6956.0
8	7320.0
9	7745.0
10	8542.0
11	9281.0
12	10003.0
13	10415.0
14	11312.0
15	11648.0
16	12708.0
17	13527.0
18	12695.0
19	12000.0
20	11870.0
21	11628.0
22	12195.0
23	13942.0
24	16247.0
25	19332.0
26	22850.0
27	27455.0
28	32805.0
29	39572.0
30	48509.0
31	60371.0
32	76041.0
33	99783.0
34	140485.0
35	209894.0
36	367594.0
37	699134.0
38	722484.0
39	87680.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79848323623143	19.159993023265248	11.925348938143674	27.116174802359648
2	15.823021704624193	20.98610929702047	36.10185503002615	27.089013968329184
3	18.03365120426864	24.01432106899685	29.18520313721466	28.766824589519846
4	12.941537427144553	16.049462722717937	35.045242270943284	35.96375757919423
5	13.867404751029872	37.21884248630186	34.77743198358822	14.136320779080048
6	34.53805569468501	35.50283036571322	16.180133573281026	13.77898036632074
7	29.13415511247178	31.54650427736683	20.948516683367526	18.370823926793864
8	27.034639128076655	34.70467150491019	19.69175013441128	18.56893923260188
9	26.55022747829843	14.545009652639962	18.93054824294952	39.97421462611208
10-11	25.77914372063317	24.965740282556354	28.159976715041367	21.095139281769104
12-13	26.013286950957287	22.448358993380488	27.708530844876332	23.829823210785893
14-15	23.105132101772917	24.157507874803077	24.618241866359973	28.119118157064033
16-17	23.682296518299513	27.21578712891337	25.58766352112106	23.51425283166606
18-19	23.6573438586887	26.6394285014194	26.66847121902124	23.03475642087066
20-21	24.068138977999705	25.96174713078168	26.569206339027613	23.400907552191
22-23	23.876421274816302	25.867210094362964	26.29681992871224	23.959548702108492
24-25	23.673822874202365	26.012270725475382	26.435638936172616	23.878267464149634
26-27	23.599977318959223	26.14408468668606	26.398318227827676	23.857619766527037
28-29	23.72134970414341	26.377646060943995	26.20611640592381	23.69488782898878
30-31	23.917586603704862	26.141655282435273	26.072303456181768	23.868454657678104
32-33	23.582932303239843	26.19498079207339	26.079557709684703	24.14252919500206
34-35	24.010301024296755	26.135279674936157	26.181556312693704	23.672862988073387
36-37	23.999609154097072	25.993935813179707	26.180397660392373	23.826057372330844
38-39	23.83081152531767	26.078526237879146	26.234605349687918	23.856056887115262
40-41	23.968531964185424	25.88213375383419	26.34770434613557	23.80162993584482
42-43	23.805266840587596	25.892706155876045	26.12199420181061	24.180032801725755
44-45	23.703983477306103	25.951990624809085	26.34737148012649	23.996654417758318
46-47	23.812633794129386	25.982096373119077	26.142332905529635	24.0629369272219
48-49	23.571784430684982	26.0671365616165	26.23755842746084	24.123520580237678
50-51	23.612705398714407	26.07397982000348	26.251309978449694	24.062004802832423
52-53	23.86391059182324	25.909315354314632	26.192099460622686	24.03467459323944
54-55	23.304260041896185	25.84132000832321	26.48908574474918	24.365334205031424
56-57	23.393399363550078	25.96378824066286	26.274857527836247	24.367954867950818
58-59	23.416835431223472	25.79602327187147	26.356604585951445	24.430536710953614
60-61	23.308534482598585	25.935610520725238	26.436926126112137	24.31892887056404
62-63	23.377441671382897	25.858259295723794	26.39564769074731	24.368651342146
64-65	23.076804902548346	25.741299411660435	26.33019608505179	24.851699600739437
66-67	23.119048495485327	25.89179908554902	26.17579626094307	24.813356158022586
68-69	23.305718816833313	25.771051812716543	26.154498906016716	24.76873046443343
70-71	23.15804085225065	25.553689186982492	26.180206995228968	25.10806296553789
72-73	23.17988162364205	25.713264654653468	26.044672540581093	25.06218118112339
74-75	23.149154372258508	25.776682165706376	25.977028606885817	25.097134855149307
76-77	23.15525962010543	25.44199067421114	26.177997720558153	25.22475198512528
78-79	23.083898288911254	25.5225371126034	26.15023863500675	25.243325963478604
80-81	23.233178313423913	25.53620339692865	25.938427713101714	25.292190576545725
82-83	23.119629142551666	25.569100524170533	25.98405831521702	25.327212018060784
84-85	23.084022143685424	25.44901272579799	26.009223383915504	25.457741746601087
86-87	23.010893447061942	25.541164139317374	25.935080398465537	25.512862015155147
88-89	22.927544012630356	25.320159558479048	25.934295188602345	25.818001240288247
90-91	22.84711180648811	25.295308608709284	25.975106571752672	25.88247301304994
92-93	22.901765039285845	25.152903043135666	26.081591174280177	25.863740743298308
94-95	22.801117450999286	24.940817052924668	26.190363276211333	26.067702219864714
96-97	22.731047193408248	24.79414276503887	26.191345793572303	26.283464247980582
98-99	22.626233716528112	24.831406559188522	26.083947568882753	26.458412155400605
100-101	22.55988478927112	24.75743089119731	26.103282962389958	26.579401357141613
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	39.5
2	59.0
3	89.5
4	76.5
5	84.5
6	161.5
7	301.0
8	474.5
9	502.5
10	405.5
11	360.5
12	358.5
13	380.0
14	405.5
15	422.5
16	489.5
17	550.0
18	598.0
19	692.0
20	818.5
21	967.0
22	1139.0
23	1383.5
24	1726.0
25	2189.0
26	2863.5
27	3950.5
28	5609.0
29	8108.5
30	11687.5
31	16758.5
32	24355.0
33	30001.0
34	33968.5
35	40434.0
36	48968.0
37	57684.5
38	65682.0
39	73270.5
40	80591.0
41	87842.0
42	94667.5
43	101832.0
44	108685.5
45	116058.5
46	121993.0
47	126142.0
48	129913.5
49	131540.0
50	131866.0
51	131860.0
52	130930.5
53	129738.5
54	127158.5
55	120735.0
56	110617.0
57	98660.0
58	88493.0
59	77182.5
60	63445.5
61	51722.0
62	41985.5
63	32020.0
64	22797.5
65	16513.0
66	12589.0
67	9882.5
68	7460.0
69	5570.0
70	4158.5
71	2973.0
72	2078.0
73	1544.5
74	1210.5
75	890.5
76	620.5
77	397.0
78	208.0
79	111.5
80	68.0
81	37.5
82	32.5
83	24.5
84	14.5
85	7.5
86	3.0
87	2.5
88	2.0
89	2.0
90	1.5
91	1.5
92	1.0
93	0.0
94	1.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15245090908599992
2	1.350019119645782E-4
3	0.009888890051405353
4	0.006918847988184633
5	0.022207814518173115
6	0.0017887753335306612
7	0.0
8	0.0
9	3.375047799114455E-5
10-11	0.045647521483023
12-13	4.725066918760237E-4
14-15	0.005011945981684965
16-17	3.375047799114455E-5
18-19	0.0014850210316103602
20-21	2.025028679468673E-4
22-23	0.0
24-25	5.231324088627405E-4
26-27	0.0031725449311675877
28-29	0.005670080302512284
30-31	0.01653773421566083
32-33	0.011542663472971436
34-35	0.010226394831316798
36-37	0.0039319306859683404
38-39	1.6875238995572275E-5
40-41	0.0035438001890701776
42-43	0.009180130013591317
44-45	0.007020099422158066
46-47	0.0038306792519949063
48-49	0.009213880491582461
50-51	0.0029025411072384315
52-53	0.002025028679468673
54-55	0.004100683075924063
56-57	0.007998863283901259
58-59	0.015542095114922067
60-61	0.0073069784850827955
62-63	0.021313426851407784
64-65	0.04036557167740888
66-67	0.06075086038406018
68-69	0.05047383983575668
70-71	0.06856409603901015
72-73	0.034155483727038284
74-75	0.02706788334889793
76-77	0.21922622979147943
78-79	0.08226679010341484
80-81	0.06787221124019169
82-83	0.027422263367804945
84-85	0.051806983716406886
86-87	0.0679228369571784
88-89	0.29595794150434657
90-91	0.2972742101460012
92-93	0.05013633505584523
94-95	0.009635761466471769
96-97	0.0145464560141833
98-99	8.100114717874692E-4
100-101	8.437619497786138E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2962921.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.35443218830289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.03953072279565	49.90819490838147
2	11.31365810530399	14.109134542576419
3	3.532094021481668	6.607251514355661
4	1.634171938682896	4.075914533385204
5	0.865357696505116	2.6979443902677125
6	0.5701687187766935	2.133152802651172
7	0.39138100975130447	1.708303844262906
8	0.27616213604337625	1.3775946547914864
9	0.21170370654818427	1.1880597972574016
>10	1.1055719250257745	11.99281564717765
>50	0.04654011850148193	1.9578728995303039
>100	0.012140489276875143	1.3254733462838961
>500	9.767644118283125E-4	0.4434265336549597
>1k	5.426468953047089E-4	0.47486058542379395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.375047799114455E-5	0.0
3	0.0	0.0	0.0	3.375047799114455E-5	0.0
4	0.0	0.0	0.0	6.75009559822891E-5	0.0
5	0.0	0.0	0.0	6.75009559822891E-5	0.0
6	0.0	0.0	0.0	6.75009559822891E-5	0.0
7	0.0	0.0	0.0	6.75009559822891E-5	0.0
8	0.0	0.0	0.0	6.75009559822891E-5	0.0
9	0.0	0.0	0.0	1.0125143397343364E-4	3.375047799114455E-5
10-11	3.375047799114455E-5	3.375047799114455E-5	0.0	1.0125143397343364E-4	3.375047799114455E-5
12-13	3.375047799114455E-5	3.375047799114455E-5	0.0	1.0125143397343364E-4	1.518771509601505E-4
14-15	3.375047799114455E-5	3.375047799114455E-5	0.0	1.0125143397343364E-4	2.1937810694243955E-4
16-17	3.375047799114455E-5	6.75009559822891E-5	0.0	1.1812667296900592E-4	2.700038239291564E-4
18-19	3.375047799114455E-5	6.75009559822891E-5	0.0	1.350019119645782E-4	3.206295409158732E-4
20-21	3.375047799114455E-5	6.75009559822891E-5	0.0	1.350019119645782E-4	3.7125525790259004E-4
22-23	3.375047799114455E-5	6.75009559822891E-5	0.0	2.0250286794686728E-4	4.3875621388487915E-4
24-25	5.062571698671682E-5	6.75009559822891E-5	0.0	3.881304968981623E-4	5.062571698671683E-4
26-27	6.75009559822891E-5	6.75009559822891E-5	0.0	7.931362327918969E-4	5.400076478583128E-4
28-29	6.75009559822891E-5	6.75009559822891E-5	0.0	0.006935723227180205	5.400076478583128E-4
30-31	6.75009559822891E-5	6.75009559822891E-5	0.0	0.031556696921720154	5.400076478583128E-4
32-33	6.75009559822891E-5	6.75009559822891E-5	0.0	0.08147365387062294	5.400076478583128E-4
34-35	6.75009559822891E-5	6.75009559822891E-5	0.0	0.15025712801657554	5.737581258494573E-4
36-37	6.75009559822891E-5	6.75009559822891E-5	0.0	0.25871428904111854	5.737581258494573E-4
38-39	6.75009559822891E-5	6.75009559822891E-5	0.0	0.4360561756455876	6.075086038406019E-4
40-41	6.75009559822891E-5	8.437619497786138E-5	0.0	0.6300876736166776	6.075086038406019E-4
42-43	6.75009559822891E-5	1.0125143397343364E-4	0.0	0.8363874703375487	6.075086038406019E-4
44-45	6.75009559822891E-5	1.0125143397343364E-4	0.0	1.0817196948551784	6.075086038406019E-4
46-47	6.75009559822891E-5	1.0125143397343364E-4	0.0	1.3740663352144726	6.075086038406019E-4
48-49	6.75009559822891E-5	1.0125143397343364E-4	0.0	1.666396100334771	6.412590818317465E-4
50-51	6.75009559822891E-5	1.0125143397343364E-4	0.0	1.9737954538781155	6.412590818317465E-4
52-53	6.75009559822891E-5	1.0125143397343364E-4	0.0	2.290273686001078	7.256352768096079E-4
54-55	6.75009559822891E-5	1.0125143397343364E-4	0.0	2.6442655744111976	7.762609937963246E-4
56-57	6.75009559822891E-5	1.350019119645782E-4	0.0	3.051886972349246	8.268867107830415E-4
58-59	6.75009559822891E-5	1.350019119645782E-4	0.0	3.474510457754358	8.60637188774186E-4
60-61	6.75009559822891E-5	1.350019119645782E-4	0.0	3.914144184067007	8.775124277697583E-4
62-63	6.75009559822891E-5	1.350019119645782E-4	0.0	4.36157427079561	9.450133837520474E-4
64-65	6.75009559822891E-5	1.350019119645782E-4	0.0	4.8475474033901005	0.0010125143397343365
66-67	6.75009559822891E-5	1.350019119645782E-4	0.0	5.405864685558609	0.0010293895787299087
68-69	6.75009559822891E-5	1.350019119645782E-4	0.0	6.002303132618115	0.001046264817725481
70-71	6.75009559822891E-5	1.518771509601505E-4	0.0	6.610284243150593	0.001046264817725481
72-73	6.75009559822891E-5	1.6875238995572276E-4	0.0	7.232491180156339	0.0010631400567210534
74-75	6.75009559822891E-5	1.6875238995572276E-4	0.0	7.881597248121027	0.0010800152957166256
76-77	6.75009559822891E-5	1.6875238995572276E-4	0.0	8.612683227126205	0.0010800152957166256
78-79	6.75009559822891E-5	1.8562762895129502E-4	0.0	9.403035045483833	0.0010800152957166256
80-81	6.75009559822891E-5	2.0250286794686728E-4	0.0	10.206482049302023	0.0010800152957166256
82-83	6.75009559822891E-5	2.0250286794686728E-4	0.0	11.026466787335876	0.0011137657737077702
84-85	6.75009559822891E-5	2.0250286794686728E-4	0.0	11.868338710346986	0.0011137657737077702
86-87	6.75009559822891E-5	2.0250286794686728E-4	0.0	12.76819395454688	0.0011306410127033424
88-89	6.75009559822891E-5	2.0250286794686728E-4	0.0	13.73934708350307	0.0011475162516989146
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1820	0.0	44.91425	1
GTATCAA	3130	0.0	40.08544	1
TCAACGC	3660	0.0	33.582077	4
CAACGCA	3840	0.0	31.906952	5
ATCAACG	3935	0.0	30.993973	3
TATCAAC	4010	0.0	30.29594	2
AACGCAG	4175	0.0	29.459503	6
GTACATG	5855	0.0	25.56882	1
ACGCAGA	4900	0.0	24.421892	7
TACATGG	6335	0.0	22.847681	2
CGCAGAG	5265	0.0	22.368052	8
GAGTACA	4465	0.0	21.394474	1
ACATGGG	6505	0.0	21.37515	3
TATACTG	1075	0.0	20.321054	5
CATGGGG	5500	0.0	19.586285	4
GCAGAGT	6245	0.0	18.17388	9
AGTACAT	5030	0.0	17.35956	2
AGAGTAC	4970	0.0	17.055483	10-11
GTACTAA	620	0.0	16.09736	1
ATACCGT	355	2.992965E-8	16.05222	6
>>END_MODULE