FastQCFastQC Report
Mon 15 Aug 2016
SRR2921668_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921668_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759639
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.037.8175961
GTATCAA37600.037.6169431
CAACGCA40250.032.7788355
TCAACGC40400.032.777224
ATCAACG42250.031.4551813
GTACATG86750.031.4049051
TACATGG88350.029.9248922
AACGCAG44100.029.9016696
TATCAAC46600.029.1315842
ACATGGG91700.027.8984663
CATGGGG81550.024.2113324
ACGCAGA53500.023.8521847
GAGTACA61250.023.402161
CGCAGAG57850.021.8134988
AGTACAT65950.019.864532
GTATAGC10200.019.5435851
ATGGGGG52400.019.2007945
TATACCG2956.413211E-817.6964175
GTGTAGC19450.017.5698471
GCAGAGT73900.016.241829
Produced by FastQC (version 0.11.5)