##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921668_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3759639
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.594474363097095	31.0	31.0	34.0	27.0	34.0
2	30.63474631473926	33.0	31.0	34.0	27.0	34.0
3	30.70512647623881	33.0	31.0	34.0	27.0	34.0
4	34.16167483101436	37.0	35.0	37.0	30.0	37.0
5	34.165946517737474	37.0	35.0	37.0	30.0	37.0
6	34.21703626332209	37.0	35.0	37.0	30.0	37.0
7	34.12603178124283	37.0	35.0	37.0	30.0	37.0
8	34.09475723600058	37.0	35.0	37.0	30.0	37.0
9	35.68078105371287	39.0	37.0	39.0	30.0	39.0
10-11	35.58478327307489	39.0	37.0	39.0	30.0	39.0
12-13	35.478902362700246	39.0	37.0	39.0	30.0	39.0
14-15	36.66223060245944	40.0	37.0	41.0	30.0	41.0
16-17	36.60347562625029	40.0	37.0	41.0	30.0	41.0
18-19	36.56234667743366	40.0	37.0	41.0	29.5	41.0
20-21	36.488004034429906	40.0	37.0	41.0	29.0	41.0
22-23	36.39613909739738	40.0	37.0	41.0	29.0	41.0
24-25	36.297333334397266	40.0	37.0	41.0	28.0	41.0
26-27	36.161581072012495	40.0	36.5	41.0	27.5	41.0
28-29	36.03206398273877	40.0	36.0	41.0	27.0	41.0
30-31	35.89505082801833	40.0	36.0	41.0	27.0	41.0
32-33	35.72586796232298	40.0	36.0	41.0	26.5	41.0
34-35	35.56744065055182	40.0	35.5	41.0	26.0	41.0
36-37	35.55077176292724	39.5	35.5	41.0	25.5	41.0
38-39	35.73919636965145	40.0	36.0	41.0	26.5	41.0
40-41	35.8110464861121	40.0	36.0	41.0	27.0	41.0
42-43	35.65887974882695	40.0	36.0	41.0	26.0	41.0
44-45	35.553191410132726	40.0	35.5	41.0	25.5	41.0
46-47	35.41156597747816	40.0	35.0	41.0	24.5	41.0
48-49	35.26405327745563	40.0	35.0	41.0	24.0	41.0
50-51	33.56274791276503	38.0	33.0	39.5	22.0	40.0
52-53	33.68508838215584	37.5	33.5	39.5	22.5	40.0
54-55	34.60918641390836	39.0	34.5	40.5	23.0	41.0
56-57	34.63313897956692	39.0	35.0	41.0	23.0	41.0
58-59	34.44666602298784	39.0	34.5	41.0	22.5	41.0
60-61	34.20062418226856	38.0	34.0	40.5	22.0	41.0
62-63	33.91344873803043	37.5	34.0	40.0	21.5	41.0
64-65	33.622549133041765	37.0	34.0	40.0	20.5	41.0
66-67	33.28318024682689	36.5	34.0	39.5	20.0	41.0
68-69	32.924370797302615	36.0	33.0	39.0	18.5	41.0
70-71	32.51189103528291	35.5	33.0	39.0	16.0	41.0
72-73	32.148130179519896	35.0	33.0	37.5	13.0	40.0
74-75	31.752037097178746	35.0	32.5	37.0	9.0	39.0
76-77	31.368547086568682	35.0	32.0	37.0	7.0	39.0
78-79	31.01837769530532	35.0	32.0	36.0	2.0	39.0
80-81	30.699839798448735	35.0	32.0	36.0	2.0	37.0
82-83	30.38039410166774	35.0	31.5	35.0	2.0	37.0
84-85	30.111131148495907	35.0	31.0	35.0	2.0	36.5
86-87	29.86934104577594	35.0	31.0	35.0	2.0	36.0
88-89	29.638004207318843	35.0	31.0	35.0	2.0	36.0
90-91	29.40795140171703	35.0	31.0	35.0	2.0	35.5
92-93	29.210674881285144	35.0	31.0	35.0	2.0	35.0
94-95	28.974165205755128	34.5	31.0	35.0	2.0	35.0
96-97	28.728441613676207	34.0	30.0	35.0	2.0	35.0
98-99	28.453694889323152	34.0	30.0	35.0	2.0	35.0
100-101	27.381633183398726	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115604.0
3	20573.0
4	14077.0
5	10525.0
6	9558.0
7	9793.0
8	10627.0
9	11096.0
10	12165.0
11	13041.0
12	13065.0
13	13091.0
14	13611.0
15	14735.0
16	14658.0
17	14941.0
18	14839.0
19	14428.0
20	14631.0
21	15276.0
22	16721.0
23	19393.0
24	22834.0
25	27036.0
26	32283.0
27	38521.0
28	45344.0
29	54091.0
30	64905.0
31	79127.0
32	98134.0
33	127866.0
34	176517.0
35	257683.0
36	436719.0
37	815646.0
38	906484.0
39	169984.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82223484242649	17.11360020097705	11.137620045331609	25.926544911264855
2	16.620697328508015	19.109642860565177	38.06740324079841	26.202256570128394
3	19.707688229169108	22.471610448291944	29.008260811239555	28.812440511299393
4	13.158738773316752	14.438480184636235	36.17672994661445	36.22605109543257
5	14.112518048799963	37.00535641805346	34.75997258578587	14.122152947360716
6	35.07406685236028	34.74022268868779	16.264807850737874	13.920902608214055
7	29.945881066034463	31.094338899872525	20.491081974330218	18.468698059762794
8	27.432296290305718	34.667752419600056	19.360977113793094	18.538974176301128
9	26.850641059743573	14.57940096823961	18.601798159280737	39.968159812736076
10-11	26.162400255057626	24.721875327949757	27.849909174958544	21.265815242034073
12-13	26.30965786083374	22.030141448915238	27.631630230839683	24.028570459411338
14-15	23.264548200593488	23.745585568133922	24.421946245577587	28.567919985695006
16-17	23.947038265971603	26.952146151207078	25.34026960300782	23.760545979813497
18-19	23.989677751609793	26.387544245760168	26.423881198911964	23.198896803718075
20-21	24.276928184856096	25.696507903063136	26.391226699378723	23.635337212702044
22-23	24.08547171834543	25.58911078004677	26.17080454222166	24.15461295938614
24-25	23.91734204393625	25.701710985313564	26.23430104555705	24.146645925193134
26-27	23.868512286551816	25.881111633878007	26.150888617262936	24.09948746230724
28-29	23.99632580227432	26.099306552372482	25.95975513860972	23.94461250674348
30-31	24.16838057914924	25.85297377504415	25.913093165236685	24.06555248056993
32-33	23.860913120909316	25.92096145431137	25.964387034305542	24.25373839047377
34-35	24.187357940754396	25.888730620425676	26.025147395818788	23.89876404300114
36-37	24.254508405312084	25.75286601705254	26.03762910392589	23.954996473709485
38-39	24.089924610634576	25.886328769983635	26.013580326547604	24.010166292834185
40-41	24.187156786639203	25.738626472282334	26.086469651729914	23.987747089348552
42-43	23.926521025698445	25.71992039466756	26.05476398991737	24.29879458971662
44-45	23.94211056187082	25.769035240438654	26.209155944487865	24.07969825320266
46-47	24.092302320684663	25.762518290683943	26.003995298394685	24.141184090236706
48-49	23.901151588630423	25.876139350660583	26.046224807874204	24.17648425283479
50-51	23.919790062790863	25.91393768461924	26.1189095141524	24.047362738437503
52-53	24.127223215034185	25.779545455454134	26.11030310477453	23.982928224737147
54-55	23.627812697610977	25.61330118028518	26.44045366982533	24.31843245227851
56-57	23.76528011132993	25.80096683358071	26.237045481532995	24.19670757355637
58-59	23.84123772623251	25.632997858130107	26.268214890727315	24.257549524910065
60-61	23.794149730807003	25.66330561962374	26.39075050943535	24.15179414013391
62-63	23.84600427610418	25.650947770251424	26.392542525486906	24.11050542815749
64-65	23.597281247680275	25.553331878918208	26.38060266893616	24.468784204465358
66-67	23.672284565831983	25.66044942304878	26.22600634295847	24.441259668160765
68-69	23.846273574584345	25.553232992571324	26.300644895459346	24.29984853738499
70-71	23.709438732068374	25.433011963876783	26.265577117876422	24.59197218617842
72-73	23.686992931694366	25.609654687150186	26.197107645070844	24.506244736084607
74-75	23.695448803031997	25.71894039946908	26.21561149046637	24.369999307032554
76-77	23.766576524451263	25.446169320074336	26.291935518689076	24.495318636785328
78-79	23.681392717342195	25.493580593440253	26.324791450920493	24.500235238297062
80-81	23.84841667831885	25.63341997936331	26.14265923823917	24.375504104078676
82-83	23.84465399801501	25.686502988817562	26.131593279819064	24.33724973334837
84-85	23.78794882293606	25.66563717555836	26.169186813983593	24.377227187521992
86-87	23.782305318226957	25.773932527889194	26.131528502828143	24.312233651055703
88-89	23.726768846860345	25.54004516261474	26.1667097675485	24.566476222976412
90-91	23.807820599284838	25.545173125353525	26.152086034830766	24.494920240530867
92-93	23.92240258865992	25.4476153354159	26.25786461150772	24.37211746441646
94-95	23.889793680424017	25.330627357136738	26.3042562202548	24.475322742184446
96-97	23.913998443641184	25.18230955702167	26.309689806783	24.59400219255414
98-99	23.883050306311734	25.193135364391317	26.21008632451914	24.71372800477781
100-101	24.113054326880643	25.100968503053117	26.289633153771558	24.496344016294678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	23.0
2	52.5
3	90.0
4	83.5
5	87.0
6	161.0
7	285.0
8	453.0
9	524.5
10	476.0
11	470.5
12	547.5
13	648.5
14	742.0
15	829.5
16	898.5
17	983.0
18	1128.0
19	1304.0
20	1426.0
21	1543.5
22	1710.5
23	1983.0
24	2286.0
25	2636.5
26	3245.0
27	4336.5
28	6015.5
29	8716.0
30	12850.5
31	18518.5
32	27098.0
33	34395.5
34	40154.5
35	48496.5
36	58799.5
37	69707.5
38	80961.5
39	90944.0
40	100177.0
41	110440.5
42	120484.5
43	130553.5
44	140336.5
45	150733.5
46	157103.5
47	161125.0
48	167346.5
49	170587.0
50	169979.0
51	168763.0
52	167773.5
53	166295.5
54	162424.0
55	153065.5
56	139684.0
57	125351.0
58	113047.5
59	99173.0
60	81290.0
61	66353.0
62	54250.0
63	41524.5
64	30133.0
65	21987.0
66	16352.0
67	12497.0
68	9397.0
69	6918.0
70	5188.5
71	3762.5
72	2693.5
73	1930.0
74	1416.0
75	1012.5
76	714.5
77	470.0
78	311.5
79	224.0
80	175.5
81	148.5
82	112.5
83	98.0
84	90.5
85	80.5
86	72.0
87	67.5
88	55.0
89	39.0
90	34.5
91	33.0
92	28.0
93	23.0
94	21.5
95	19.0
96	14.5
97	9.5
98	10.0
99	11.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05309020360731442
2	0.0741294576420768
3	0.06976733670440168
4	0.06995352479320488
5	0.06553820725872883
6	0.010639319360183253
7	0.013591730482634103
8	0.017288893960297785
9	0.006356993317709492
10-11	0.012993268768623795
12-13	0.02291443407199468
14-15	0.040190028883092234
16-17	0.027622332888875768
18-19	0.01012065254137432
20-21	0.02074667275235734
22-23	0.0369716347766368
24-25	0.034777275158599005
26-27	0.021105749780763527
28-29	0.011995832578606617
30-31	0.012035730026207303
32-33	0.008883831665753015
34-35	0.0054260528736934585
36-37	5.984617140103079E-4
38-39	0.004162633699671697
40-41	0.0011304276820194705
42-43	0.22964970838955548
44-45	0.19912816097502978
46-47	0.2055782483371409
48-49	0.21266669486086298
50-51	0.20374296574750925
52-53	0.2114431731344419
54-55	0.20724064198716952
56-57	0.21032604460162266
58-59	0.2192763720133768
60-61	0.22414386062066066
62-63	0.20904932627840064
64-65	0.2086503518023938
66-67	0.20645599218435598
68-69	0.20102993931066254
70-71	0.19310364638732602
72-73	0.20424833341711798
74-75	0.20371636744910882
76-77	0.19735937413139934
78-79	0.21608457620532184
80-81	0.2275085453683186
82-83	0.22581955341988955
84-85	0.21999452607018918
86-87	0.2403821217941403
88-89	0.2327085127056082
90-91	0.23987675412453163
92-93	0.23454379529523978
94-95	0.2374829072684904
96-97	0.24519641380462326
98-99	0.2384936426077078
100-101	0.24502352486502027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3759639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.51199053099793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91361150464887	44.234548965602194
2	12.547550778275246	14.432692430947633
3	4.264931159851975	7.3585404144229445
4	1.9853871420082567	4.567342660461756
5	1.105762625016202	3.1797304809731615
6	0.6840624525490275	2.360507597616653
7	0.46684162950605634	1.8794293962939568
8	0.3517452255039284	1.6183654462804529
9	0.24265522531044909	1.2560026518316543
>10	1.3465662058770125	13.546123467014306
>50	0.06857621343262117	2.65848918944224
>100	0.02073220684321173	1.9760287837316735
>500	9.744192759022606E-4	0.3876688464786453
>1k	6.032119013089061E-4	0.544529668902729
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.979489520137439E-5
2	0.0	0.0	0.0	0.0	7.979489520137439E-5
3	0.0	0.0	0.0	0.0	7.979489520137439E-5
4	0.0	0.0	0.0	2.659829840045813E-5	7.979489520137439E-5
5	0.0	7.979489520137439E-5	0.0	2.659829840045813E-5	7.979489520137439E-5
6	0.0	7.979489520137439E-5	0.0	2.659829840045813E-5	7.979489520137439E-5
7	0.0	7.979489520137439E-5	0.0	2.659829840045813E-5	7.979489520137439E-5
8	0.0	7.979489520137439E-5	0.0	2.659829840045813E-5	7.979489520137439E-5
9	0.0	7.979489520137439E-5	0.0	2.659829840045813E-5	7.979489520137439E-5
10-11	0.0	7.979489520137439E-5	0.0	6.649574600114532E-5	7.979489520137439E-5
12-13	0.0	1.0639319360183252E-4	0.0	7.979489520137439E-5	9.309404440160346E-5
14-15	0.0	1.3299149200229063E-4	0.0	9.309404440160346E-5	1.1969234280206158E-4
16-17	0.0	1.5958979040274878E-4	0.0	1.0639319360183252E-4	1.4629064120251972E-4
18-19	0.0	1.9948723800343595E-4	0.0	1.0639319360183252E-4	1.5958979040274878E-4
20-21	0.0	2.1278638720366504E-4	0.0	1.3299149200229063E-4	1.861880888032069E-4
22-23	0.0	2.1278638720366504E-4	0.0	2.1278638720366504E-4	2.7928213320481035E-4
24-25	0.0	2.5268383480435224E-4	0.0	3.856753268066429E-4	3.723761776064138E-4
26-27	0.0	2.6598298400458127E-4	0.0	7.846498028135148E-4	3.723761776064138E-4
28-29	0.0	2.7928213320481035E-4	0.0	0.005718634156098498	3.723761776064138E-4
30-31	0.0	2.9258128240503944E-4	0.0	0.023938468560412318	3.723761776064138E-4
32-33	0.0	2.9258128240503944E-4	0.0	0.05785129902099643	3.9897447600687196E-4
34-35	0.0	2.9258128240503944E-4	0.0	0.10108683307094113	3.9897447600687196E-4
36-37	0.0	3.1917958080549756E-4	0.0	0.17258305917137257	3.9897447600687196E-4
38-39	0.0	3.7237617760641384E-4	0.0	0.2937649066838598	3.9897447600687196E-4
40-41	0.0	3.9897447600687196E-4	0.0	0.4287379719169846	4.1227362520710104E-4
42-43	0.0	4.255727744073301E-4	0.0	0.5674613972245739	4.654702220080173E-4
44-45	0.0	4.255727744073301E-4	0.0	0.7336209673322358	5.186668188089335E-4
46-47	0.0	4.255727744073301E-4	0.0	0.9297834180356146	5.452651172093916E-4
48-49	0.0	4.255727744073301E-4	0.0	1.136438897457974	5.585642664096207E-4
50-51	0.0	4.388719236075591E-4	0.0	1.3591464499650099	5.585642664096207E-4
52-53	0.0	4.521710728077882E-4	0.0	1.5803910960600205	6.649574600114533E-4
54-55	0.0	4.654702220080173E-4	0.0	1.8243905864366234	7.314532060125985E-4
56-57	0.0	4.787693712082463E-4	0.0	2.1058803784086715	7.447523552128276E-4
58-59	0.0	4.787693712082463E-4	0.0	2.406760329914654	7.580515044130567E-4
60-61	0.0	4.787693712082463E-4	0.0	2.7311797755050415	7.713506536132857E-4
62-63	2.659829840045813E-5	4.787693712082463E-4	0.0	3.0635255140187665	8.511455488146602E-4
64-65	2.659829840045813E-5	5.053676696087045E-4	0.0	3.4049944688838476	8.511455488146602E-4
66-67	2.659829840045813E-5	5.053676696087045E-4	0.0	3.7938083949017445	8.777438472151182E-4
68-69	2.659829840045813E-5	5.053676696087045E-4	0.0	4.220498297841894	8.777438472151182E-4
70-71	2.659829840045813E-5	5.186668188089335E-4	0.0	4.674837131969319	9.043421456155764E-4
72-73	2.659829840045813E-5	5.319659680091625E-4	0.0	5.147103219218653	0.001063931936018325
74-75	2.659829840045813E-5	5.319659680091625E-4	0.0	5.617480827281556	0.0011038293836190124
76-77	2.659829840045813E-5	5.319659680091625E-4	0.0	6.13159933706401	0.0011171285328192414
78-79	2.659829840045813E-5	5.452651172093916E-4	0.0	6.716323029950482	0.0011171285328192414
80-81	2.659829840045813E-5	5.984617140103079E-4	0.0	7.321846592186112	0.0011304276820194705
82-83	2.659829840045813E-5	6.117608632105369E-4	0.0	7.9496728276305255	0.0012767183232219902
84-85	2.659829840045813E-5	6.117608632105369E-4	0.0	8.578602892458559	0.0012767183232219902
86-87	2.659829840045813E-5	6.383591616109951E-4	0.0	9.240435052407957	0.0013166157708226775
88-89	2.659829840045813E-5	6.383591616109951E-4	0.0	9.963562991021213	0.0013565132184233646
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2485	0.0	37.817596	1
GTATCAA	3760	0.0	37.616943	1
CAACGCA	4025	0.0	32.778835	5
TCAACGC	4040	0.0	32.77722	4
ATCAACG	4225	0.0	31.455181	3
GTACATG	8675	0.0	31.404905	1
TACATGG	8835	0.0	29.924892	2
AACGCAG	4410	0.0	29.901669	6
TATCAAC	4660	0.0	29.131584	2
ACATGGG	9170	0.0	27.898466	3
CATGGGG	8155	0.0	24.211332	4
ACGCAGA	5350	0.0	23.852184	7
GAGTACA	6125	0.0	23.40216	1
CGCAGAG	5785	0.0	21.813498	8
AGTACAT	6595	0.0	19.86453	2
GTATAGC	1020	0.0	19.543585	1
ATGGGGG	5240	0.0	19.200794	5
TATACCG	295	6.413211E-8	17.696417	5
GTGTAGC	1945	0.0	17.569847	1
GCAGAGT	7390	0.0	16.24182	9
>>END_MODULE