FastQCFastQC Report
Mon 15 Aug 2016
SRR2921668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759639
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.039.1082421
GTATCAA38050.038.109651
TCAACGC41500.033.0521244
CAACGCA42300.032.5673875
ATCAACG44650.030.7215653
AACGCAG46300.029.8508286
TATCAAC48600.029.005952
GTACATG86700.028.734391
TACATGG87250.027.4177652
ACATGGG88050.026.3061583
CATGGGG76300.023.6378884
ACGCAGA59100.022.8225237
ATACCGT3750.021.5308916
CGCAGAG63350.020.8415978
GAGTACA62850.019.894831
ATGGGGG46850.019.4678045
CCCTATA6000.017.403572
CATACCG3059.283394E-817.1323995
TATACCG3059.283394E-817.1323995
GCACCGT8200.016.7968776
Produced by FastQC (version 0.11.5)