##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921668_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3759639
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46660942712851	33.0	31.0	34.0	29.0	34.0
2	31.591640846368495	34.0	31.0	34.0	29.0	34.0
3	31.671572456823647	34.0	31.0	34.0	29.0	34.0
4	35.136976183085665	37.0	35.0	37.0	33.0	37.0
5	34.88606858264849	37.0	35.0	37.0	32.0	37.0
6	34.866276256842745	37.0	35.0	37.0	32.0	37.0
7	34.7504946618545	37.0	35.0	37.0	32.0	37.0
8	34.69613651736244	37.0	35.0	37.0	32.0	37.0
9	36.30729705697808	39.0	37.0	39.0	32.0	39.0
10-11	36.201430243701594	39.0	37.0	39.0	32.0	39.0
12-13	36.12056051126186	39.0	37.0	39.0	32.0	39.0
14-15	37.39047884650627	40.0	38.0	41.0	32.0	41.0
16-17	37.35615786515673	40.0	38.0	41.0	32.0	41.0
18-19	37.308113092773	40.0	38.0	41.0	31.5	41.0
20-21	37.24882455469794	40.0	38.0	41.0	31.0	41.0
22-23	37.16224536451505	40.0	38.0	41.0	31.0	41.0
24-25	37.08441102988877	40.0	38.0	41.0	31.0	41.0
26-27	36.962889389114224	40.0	37.0	41.0	30.5	41.0
28-29	36.848291950370765	40.0	37.0	41.0	30.0	41.0
30-31	36.70044424477989	40.0	37.0	41.0	30.0	41.0
32-33	36.65719341670836	40.0	37.0	41.0	30.0	41.0
34-35	36.79914840228011	40.0	37.0	41.0	30.0	41.0
36-37	36.8050683855551	40.0	37.0	41.0	30.0	41.0
38-39	36.70654190468819	40.0	37.0	41.0	30.0	41.0
40-41	36.631210469941394	40.0	37.0	41.0	30.0	41.0
42-43	36.51707836842846	40.0	37.0	41.0	30.0	41.0
44-45	36.40118984828065	40.0	37.0	41.0	29.5	41.0
46-47	36.259671340785644	40.0	36.0	41.0	29.0	41.0
48-49	36.121577497201194	40.0	36.0	41.0	28.5	41.0
50-51	35.969330965020845	40.0	36.0	41.0	28.0	41.0
52-53	35.79758269344477	39.5	35.0	41.0	28.0	41.0
54-55	35.585249408254356	39.0	35.0	41.0	28.0	41.0
56-57	35.364259706849516	39.0	35.0	41.0	27.0	41.0
58-59	35.123628890965335	39.0	35.0	41.0	26.5	41.0
60-61	34.846032823896124	38.0	35.0	40.0	26.0	41.0
62-63	34.54142259403097	37.5	34.5	40.0	26.0	41.0
64-65	34.203870637579826	37.0	34.0	40.0	26.0	41.0
66-67	33.84943434728707	36.5	34.0	39.5	25.5	41.0
68-69	33.48375402531998	36.0	34.0	39.0	25.0	41.0
70-71	33.08390898700647	35.5	33.5	39.0	24.0	40.0
72-73	32.720000909661806	35.0	33.0	37.5	23.0	40.0
74-75	32.31557431444881	35.0	33.0	37.0	22.5	39.0
76-77	29.603438122649543	33.0	29.0	34.5	17.5	36.5
78-79	31.17656096768865	34.5	31.5	36.0	19.5	37.5
80-81	31.35270779987121	35.0	32.0	36.0	20.0	37.0
82-83	31.17451130281391	35.0	32.0	35.0	20.0	37.0
84-85	30.972337769663525	35.0	32.0	35.0	19.0	36.5
86-87	30.76440730080734	35.0	32.0	35.0	18.0	36.0
88-89	30.496223972567577	35.0	32.0	35.0	12.5	36.0
90-91	30.29891061349241	35.0	32.0	35.0	8.5	35.0
92-93	30.141267685541084	34.0	32.0	35.0	4.5	35.0
94-95	30.03964715761274	34.0	32.0	35.0	2.0	35.0
96-97	29.84503565368909	34.0	31.0	35.0	2.0	35.0
98-99	29.64633107061609	34.0	31.0	35.0	2.0	35.0
100-101	28.523603994958027	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	66534.0
3	23688.0
4	12952.0
5	8976.0
6	7810.0
7	7715.0
8	7906.0
9	8682.0
10	9359.0
11	10531.0
12	10976.0
13	11620.0
14	12372.0
15	13359.0
16	13996.0
17	14730.0
18	14432.0
19	13481.0
20	13077.0
21	12909.0
22	13733.0
23	15621.0
24	18355.0
25	22001.0
26	26385.0
27	32013.0
28	38621.0
29	46554.0
30	56870.0
31	71011.0
32	90465.0
33	119089.0
34	170278.0
35	255248.0
36	458151.0
37	910941.0
38	990718.0
39	128478.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.888870416669334	18.39196309250532	11.666112500079928	27.05305399074542
2	15.840391443503776	20.238039824737747	36.56938474948153	27.352183982276944
3	18.427583198221985	23.395070020708946	28.828129052509215	29.349217728559857
4	12.883044088789914	15.451571810434189	35.09331697607405	36.572067124701846
5	13.882870265921445	37.25861360724335	34.84456344527263	14.01395268156258
6	35.143069477057495	35.248161226863054	15.923674290584142	13.685095005495306
7	29.776324209937233	31.249285170540354	20.448803847604477	18.525586771917936
8	27.402178773015173	35.09895497945414	19.080847921835048	18.41801832569563
9	26.68943126971267	14.491129199140982	18.42464620263972	40.39479332850663
10-11	26.118092376234948	24.923362647062618	27.774249556688225	21.184295420014205
12-13	26.20961371198762	22.17369794368063	27.61229163508111	24.004396709250646
14-15	23.153634451241693	23.900779010952807	24.363373704081173	28.582212833724324
16-17	23.933055806687822	27.131527787641314	25.241346310111158	23.69407009555971
18-19	23.984385504404944	26.573023314949644	26.275543554226154	23.167047626419258
20-21	24.268262532714317	25.806764787124692	26.303888108153185	23.62108457200781
22-23	24.02500612425821	25.706829831268372	26.08968573844457	24.17847830602885
24-25	23.861614884892745	25.86681540494475	26.13337131585499	24.138198394307516
26-27	23.84571107559853	26.026361175717057	26.095797788242404	24.03212996044201
28-29	23.925877583287573	26.24770593516915	25.90672334725255	23.91969313429072
30-31	24.119522722097248	25.96988973616275	25.85803770380507	24.05254983793493
32-33	23.823982340881322	26.057960343063176	25.863157791131126	24.25489952492438
34-35	24.094927294124158	26.032478235055024	26.008258017011876	23.864336453808946
36-37	24.18877380839017	25.913527192755197	25.997820451369254	23.89987854748538
38-39	24.051125653287457	26.016620212738513	25.958822110314316	23.97343202365972
40-41	24.131471545925386	25.888098847042517	26.000094959297172	23.98033464773492
42-43	23.932570416376777	25.866720648354836	25.94451439689761	24.256194538370778
44-45	23.890006765781983	25.926391430878432	26.11601154824686	24.067590255092725
46-47	24.091946037109363	25.887845209594495	25.917423536235244	24.102785217060894
48-49	23.82459183563538	26.022460827486388	25.981575561730168	24.171371775148064
50-51	23.845036686717904	26.02489403609755	26.02293900708465	24.10713027009989
52-53	24.11696706884908	25.897090386110545	25.935858107214894	24.050084437825486
54-55	23.55748117493815	25.79862709846008	26.304960017108737	24.338931709493032
56-57	23.664606938312822	25.911173433348022	26.108616344814994	24.315603283524165
58-59	23.701451839240228	25.81952767007402	26.120523273987274	24.35849721669848
60-61	23.704607720449804	25.826448616763493	26.21984072527364	24.249102937513058
62-63	23.713864722327642	25.829202038570987	26.173114120308572	24.2838191187928
64-65	23.427674462169882	25.711164046168456	26.204889006680038	24.656272484981628
66-67	23.476308932904246	25.89203884384042	26.037810371656178	24.593841851599155
68-69	23.682431349057076	25.7313180137429	26.064465016105142	24.52178562109488
70-71	23.444946402702723	25.60053660513408	26.08553075930558	24.868986232857615
72-73	23.455369735162147	25.757981927290093	25.935694380345893	24.85095395720187
74-75	23.40377499853003	25.779531635826487	25.963551041073856	24.853142324569628
76-77	23.364906059620054	25.51337727486187	26.13424342693269	24.987473238585384
78-79	23.305157560132354	25.525259230379838	26.165453546179485	25.004129663308316
80-81	23.409170094252968	25.629149402305075	25.933486593220103	25.02819391022186
82-83	23.308611053243098	25.641995736125047	25.98958961068262	25.059803599949237
84-85	23.227271426889136	25.580056059234106	25.99576994290953	25.19690257096722
86-87	23.12601442729208	25.704995360716904	25.94859099409483	25.22039921789619
88-89	23.055613616970646	25.47392231751705	25.98560382219148	25.484860243320828
90-91	23.012045733796498	25.405785329792664	26.068680198380235	25.513488738030603
92-93	23.002670327166975	25.350463807672575	26.140078539660504	25.506787325499946
94-95	22.869147467460436	25.169341388088647	26.252211200578834	25.709299943872082
96-97	22.812932828190593	25.013277886987723	26.254768633567426	25.919020651254254
98-99	22.64066605942194	25.044974810515306	26.211028314281464	26.10333081578129
100-101	22.618500611429443	24.978797794731268	26.249879476252	26.15282211758729
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	23.0
2	47.5
3	64.5
4	52.5
5	65.5
6	126.5
7	240.0
8	356.5
9	383.0
10	348.5
11	314.0
12	327.0
13	372.0
14	399.0
15	422.0
16	472.5
17	524.0
18	604.0
19	696.0
20	768.0
21	891.5
22	1116.0
23	1438.0
24	1811.0
25	2335.5
26	3065.0
27	4199.5
28	6026.0
29	8888.0
30	13083.5
31	18802.5
32	27751.0
33	34983.5
34	40301.5
35	48402.0
36	58533.0
37	69118.0
38	80087.5
39	90481.0
40	99924.5
41	109705.5
42	119381.5
43	129822.0
44	139649.5
45	149922.5
46	156766.0
47	161266.0
48	167900.0
49	171354.0
50	170932.0
51	170205.5
52	169537.5
53	168412.0
54	164392.5
55	155105.0
56	142564.5
57	127783.5
58	114002.0
59	99754.0
60	82294.5
61	67096.0
62	54236.5
63	40756.0
64	29238.5
65	21146.0
66	15531.0
67	11965.5
68	9033.5
69	6532.5
70	4786.5
71	3404.0
72	2424.0
73	1696.5
74	1174.5
75	764.0
76	480.0
77	307.0
78	171.5
79	101.5
80	56.0
81	34.0
82	26.0
83	18.5
84	11.0
85	7.0
86	8.5
87	9.0
88	5.5
89	3.0
90	4.0
91	3.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1663723564948656
2	4.255727744073301E-4
3	0.010479729569780504
4	0.006596378003313617
5	0.02486940900442835
6	0.0017820859928306947
7	2.659829840045813E-5
8	0.0
9	2.659829840045813E-5
10-11	0.04903396310124457
12-13	2.3938468560412315E-4
14-15	0.00512017244208819
16-17	0.0
18-19	0.0014762055612254261
20-21	2.2608553640389412E-4
22-23	0.0
24-25	3.9897447600687196E-4
26-27	0.002526838348043522
28-29	0.005572343514895978
30-31	0.017421885452300074
32-33	0.011916037683405243
34-35	0.010187148287375464
36-37	0.0038567532680664285
38-39	0.0
40-41	0.0035508728364611604
42-43	0.009030122306955534
44-45	0.0074608227013285055
46-47	0.0034444796428593277
48-49	0.009003524008555077
50-51	0.0027662230336476454
52-53	0.001808684291231153
54-55	0.004149334550471468
56-57	0.008631147830948663
58-59	0.01633135521788129
60-61	0.007593814193330797
62-63	0.022063288523180017
64-65	0.04189231998072156
66-67	0.0635566340278947
68-69	0.05289071636931099
70-71	0.07145632865283078
72-73	0.03512305303780496
74-75	0.028406982691689283
76-77	0.23059394798277175
78-79	0.08722911960430244
80-81	0.06974073840600122
82-83	0.028885752062897528
84-85	0.05336948574051924
86-87	0.06896938775238794
88-89	0.2982600191135372
90-91	0.30275513154321465
92-93	0.0544467168257378
94-95	0.00981477210976905
96-97	0.015147730939060904
98-99	9.176412948158054E-4
100-101	1.7288893960297784E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3759639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.84398321543173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27070961608014	43.35530937245527
2	12.80249036796869	14.554890951850775
3	4.455013462964246	7.59722131539786
4	2.076209063671762	4.720799726683396
5	1.1085024086548823	3.150584615592189
6	0.7046070675662427	2.403160339332604
7	0.49554921074181807	1.9718293712479844
8	0.3527615142176965	1.6041895674592819
9	0.25956880066152427	1.327943209324809
>10	1.3825960727889337	13.781555841742454
>50	0.06991134004683767	2.680138435969254
>100	0.020392073300774298	1.8981488796960142
>500	0.0010790842036861106	0.4307895803887848
>1k	6.099171328064407E-4	0.523438792859285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.659829840045813E-5
2	0.0	0.0	0.0	0.0	2.659829840045813E-5
3	0.0	0.0	0.0	0.0	2.659829840045813E-5
4	0.0	0.0	0.0	0.0	2.659829840045813E-5
5	0.0	0.0	0.0	0.0	2.659829840045813E-5
6	0.0	0.0	0.0	0.0	5.319659680091626E-5
7	0.0	2.659829840045813E-5	0.0	0.0	5.319659680091626E-5
8	2.659829840045813E-5	5.319659680091626E-5	0.0	0.0	5.319659680091626E-5
9	2.659829840045813E-5	5.319659680091626E-5	0.0	0.0	5.319659680091626E-5
10-11	2.659829840045813E-5	5.319659680091626E-5	0.0	1.3299149200229065E-5	5.319659680091626E-5
12-13	2.659829840045813E-5	5.319659680091626E-5	0.0	2.659829840045813E-5	7.979489520137439E-5
14-15	2.659829840045813E-5	7.979489520137439E-5	0.0	3.9897447600687195E-5	1.0639319360183252E-4
16-17	2.659829840045813E-5	1.0639319360183252E-4	0.0	5.319659680091626E-5	1.3299149200229063E-4
18-19	2.659829840045813E-5	1.0639319360183252E-4	0.0	9.309404440160346E-5	1.5958979040274878E-4
20-21	2.659829840045813E-5	1.0639319360183252E-4	0.0	1.5958979040274878E-4	1.5958979040274878E-4
22-23	2.659829840045813E-5	1.0639319360183252E-4	0.0	2.260855364038941E-4	1.7288893960297784E-4
24-25	2.659829840045813E-5	1.0639319360183252E-4	0.0	3.590770284061847E-4	1.861880888032069E-4
26-27	2.659829840045813E-5	1.0639319360183252E-4	0.0	7.314532060125985E-4	1.861880888032069E-4
28-29	2.659829840045813E-5	1.0639319360183252E-4	0.0	0.005758531603699185	1.861880888032069E-4
30-31	2.659829840045813E-5	1.0639319360183252E-4	0.0	0.024071460052414607	1.9948723800343595E-4
32-33	3.9897447600687195E-5	1.0639319360183252E-4	0.0	0.05810398285580079	2.1278638720366504E-4
34-35	5.319659680091626E-5	1.0639319360183252E-4	0.0	0.10152570499454867	2.3938468560412315E-4
36-37	5.319659680091626E-5	1.0639319360183252E-4	0.0	0.17304852939338058	2.3938468560412315E-4
38-39	5.319659680091626E-5	1.1969234280206158E-4	0.0	0.2936851117886584	2.6598298400458127E-4
40-41	5.319659680091626E-5	1.3299149200229063E-4	0.0	0.42889756170738735	2.6598298400458127E-4
42-43	5.319659680091626E-5	1.3299149200229063E-4	0.0	0.5675677904181757	2.6598298400458127E-4
44-45	5.319659680091626E-5	1.3299149200229063E-4	0.0	0.7336209673322358	2.6598298400458127E-4
46-47	5.319659680091626E-5	1.3299149200229063E-4	0.0	0.9293844435596077	2.6598298400458127E-4
48-49	5.319659680091626E-5	1.3299149200229063E-4	0.0	1.134975991045949	3.723761776064138E-4
50-51	5.319659680091626E-5	1.5958979040274878E-4	0.0	1.3566196116169664	3.723761776064138E-4
52-53	5.319659680091626E-5	1.5958979040274878E-4	0.0	1.5770663087599632	3.989744760068719E-4
54-55	5.319659680091626E-5	2.3938468560412315E-4	0.0	1.8199353714545465	4.521710728077882E-4
56-57	5.319659680091626E-5	2.3938468560412315E-4	0.0	2.100308034893776	4.654702220080173E-4
58-59	6.649574600114532E-5	2.6598298400458127E-4	0.0	2.4000442595685385	4.787693712082463E-4
60-61	7.979489520137439E-5	2.9258128240503944E-4	0.0	2.722708217464496	4.787693712082463E-4
62-63	7.979489520137439E-5	2.9258128240503944E-4	0.0	3.0544687934134105	5.053676696087045E-4
64-65	7.979489520137439E-5	3.5907702840618476E-4	0.0	3.395379184012082	5.053676696087045E-4
66-67	7.979489520137439E-5	4.521710728077882E-4	0.0	3.7822913316943465	5.053676696087045E-4
68-69	9.309404440160346E-5	4.521710728077882E-4	0.0	4.207106054597263	5.053676696087045E-4
70-71	1.0639319360183252E-4	4.521710728077882E-4	0.0	4.659277127405051	5.186668188089335E-4
72-73	1.0639319360183252E-4	4.521710728077882E-4	0.0	5.1303329920771645	6.915557584119113E-4
74-75	1.0639319360183252E-4	4.521710728077882E-4	0.0	5.60091008737807	6.915557584119113E-4
76-77	1.0639319360183252E-4	4.521710728077882E-4	0.0	6.116105828245743	6.915557584119113E-4
78-79	1.0639319360183252E-4	4.521710728077882E-4	0.0	6.70286429095985	7.048549076121404E-4
80-81	1.0639319360183252E-4	4.787693712082463E-4	0.0	7.311154076229127	7.447523552128277E-4
82-83	1.0639319360183252E-4	4.787693712082463E-4	0.0	7.94005754275876	8.777438472151182E-4
84-85	1.0639319360183252E-4	4.787693712082463E-4	0.0	8.569798855688006	8.777438472151182E-4
86-87	1.0639319360183252E-4	4.787693712082463E-4	0.0	9.234543529312255	9.309404440160344E-4
88-89	1.0639319360183252E-4	4.787693712082463E-4	0.0	9.959812630946749	0.001010735339217409
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2480	0.0	39.108242	1
GTATCAA	3805	0.0	38.10965	1
TCAACGC	4150	0.0	33.052124	4
CAACGCA	4230	0.0	32.567387	5
ATCAACG	4465	0.0	30.721565	3
AACGCAG	4630	0.0	29.850828	6
TATCAAC	4860	0.0	29.00595	2
GTACATG	8670	0.0	28.73439	1
TACATGG	8725	0.0	27.417765	2
ACATGGG	8805	0.0	26.306158	3
CATGGGG	7630	0.0	23.637888	4
ACGCAGA	5910	0.0	22.822523	7
ATACCGT	375	0.0	21.530891	6
CGCAGAG	6335	0.0	20.841597	8
GAGTACA	6285	0.0	19.89483	1
ATGGGGG	4685	0.0	19.467804	5
CCCTATA	600	0.0	17.40357	2
CATACCG	305	9.283394E-8	17.132399	5
TATACCG	305	9.283394E-8	17.132399	5
GCACCGT	820	0.0	16.796877	6
>>END_MODULE