FastQCFastQC Report
Mon 15 Aug 2016
SRR2921667_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921667_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3602076
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.045.4782641
GTATCAA40150.038.7793581
TCAACGC44550.034.0954554
CAACGCA46400.032.7346845
GTACATG91600.031.870731
AACGCAG49750.030.4193956
ATCAACG50550.029.8602983
TACATGG96750.029.5867162
ACATGGG99650.027.7225973
TATCAAC55200.027.6915452
ACGCAGA60350.024.6078857
CATGGGG86150.024.5738834
GAGTACA68050.023.8566951
CGCAGAG63750.022.6275128
AGTACAT69950.020.9701352
GTATTAG7850.020.5599291
ATGGGGG49150.019.9904675
GTATAGA10200.018.1500051
GCAGAGT77250.017.8749149
ACCGTAT4253.6379788E-1217.8638258
Produced by FastQC (version 0.11.5)