##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921667_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3602076
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.342971664118135	31.0	31.0	34.0	26.0	34.0
2	30.374176447137707	31.0	31.0	34.0	26.0	34.0
3	30.44107981064253	33.0	31.0	34.0	26.0	34.0
4	33.894479461288434	37.0	35.0	37.0	30.0	37.0
5	33.89230071769724	37.0	35.0	37.0	30.0	37.0
6	33.941342159354775	37.0	35.0	37.0	30.0	37.0
7	33.84754041835875	37.0	35.0	37.0	29.0	37.0
8	33.81803993030686	37.0	35.0	37.0	29.0	37.0
9	35.37611477381377	39.0	37.0	39.0	30.0	39.0
10-11	35.271957893170494	39.0	35.0	39.0	29.0	39.0
12-13	35.155961589927585	39.0	35.0	39.0	28.0	39.0
14-15	36.30373609551825	40.0	37.0	41.0	28.0	41.0
16-17	36.23994760243815	40.0	37.0	41.0	27.0	41.0
18-19	36.198647807542095	40.0	36.5	41.0	27.0	41.0
20-21	36.114907209064995	40.0	36.5	41.0	27.0	41.0
22-23	36.01235454221399	40.0	36.0	41.0	27.0	41.0
24-25	35.90373412443269	40.0	36.0	41.0	27.0	41.0
26-27	35.75971523088353	40.0	36.0	41.0	27.0	41.0
28-29	35.62109891629161	40.0	36.0	41.0	26.0	41.0
30-31	35.47690442955673	40.0	36.0	41.0	25.0	41.0
32-33	35.30914186707888	39.5	35.0	41.0	25.0	41.0
34-35	35.140316167676644	39.0	35.0	41.0	24.5	41.0
36-37	35.1149346932158	39.0	35.0	41.0	24.5	41.0
38-39	35.29790168225212	39.5	35.0	41.0	24.5	41.0
40-41	35.364627648056285	40.0	35.0	41.0	24.5	41.0
42-43	35.20838760759074	40.0	35.0	41.0	24.0	41.0
44-45	35.092172958038645	40.0	35.0	41.0	23.5	41.0
46-47	34.93531521822416	40.0	35.0	41.0	23.0	41.0
48-49	34.76924223697668	39.0	35.0	41.0	22.0	41.0
50-51	33.060884056860544	37.0	32.5	39.5	18.5	40.0
52-53	33.17519535956487	37.5	32.5	39.5	18.0	40.0
54-55	34.08108754507123	38.5	34.0	40.5	20.0	41.0
56-57	34.087234694659415	38.5	34.0	41.0	19.5	41.0
58-59	33.88359657042217	38.0	34.0	40.5	18.0	41.0
60-61	33.62172411131803	37.5	34.0	40.0	17.0	41.0
62-63	33.32099877959266	37.0	33.5	40.0	13.5	41.0
64-65	33.02033688350829	36.5	33.0	40.0	12.5	41.0
66-67	32.6753438850263	36.0	33.0	39.0	9.0	41.0
68-69	32.30661068783668	35.5	33.0	39.0	7.5	41.0
70-71	31.90230453216423	35.0	32.0	38.5	4.0	40.0
72-73	31.547697216827185	35.0	32.0	37.0	2.0	39.5
74-75	31.15825540604918	35.0	32.0	37.0	2.0	39.0
76-77	30.790530516291163	35.0	32.0	36.0	2.0	39.0
78-79	30.458965191184195	35.0	31.0	36.0	2.0	37.5
80-81	30.152981919315415	35.0	31.0	35.5	2.0	37.0
82-83	29.848408251241786	35.0	31.0	35.0	2.0	37.0
84-85	29.594924565722657	35.0	31.0	35.0	2.0	36.0
86-87	29.361884091285134	35.0	31.0	35.0	2.0	36.0
88-89	29.135334457129723	35.0	30.0	35.0	2.0	36.0
90-91	28.910201506020417	34.0	30.0	35.0	2.0	35.0
92-93	28.706531733367093	34.0	30.0	35.0	2.0	35.0
94-95	28.46860407720437	34.0	30.0	35.0	2.0	35.0
96-97	28.217268736139935	34.0	29.5	35.0	2.0	35.0
98-99	27.93355109664538	34.0	29.0	35.0	2.0	35.0
100-101	26.850231366578605	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128596.0
3	22165.0
4	14982.0
5	11482.0
6	10363.0
7	10620.0
8	11253.0
9	11665.0
10	12830.0
11	13771.0
12	13620.0
13	13536.0
14	14123.0
15	14847.0
16	14841.0
17	15164.0
18	14907.0
19	14533.0
20	14723.0
21	15750.0
22	17292.0
23	20059.0
24	23556.0
25	27793.0
26	32578.0
27	39222.0
28	45917.0
29	55044.0
30	65360.0
31	79896.0
32	99418.0
33	129108.0
34	177437.0
35	257964.0
36	437083.0
37	788662.0
38	784931.0
39	126969.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.975201928738876	16.889991889519703	11.101969846789695	26.032836334951725
2	16.96261906832293	19.04301392217661	37.49350258604715	26.500864423453308
3	20.009862097320845	22.17577313286884	28.72376126501539	29.090603504794927
4	13.555722361062186	14.221617104688544	35.3027001379016	36.91996039634766
5	14.477438051103638	36.68656394677293	34.23002581794322	14.605972184180219
6	35.47208000621925	34.0192185955599	16.099891163110957	14.408810235109893
7	30.43747823909206	30.820914460682097	20.04588462706732	18.69572267315852
8	27.961314556493512	33.978405412474615	19.207970771837406	18.85230925919447
9	27.190134041111865	14.493403444867681	18.374015791753195	39.94244672226726
10-11	26.560031496885017	24.419331712954094	27.36075317092891	21.65988361923198
12-13	26.65029661577012	21.697587976759454	27.25210840998175	24.40000699748868
14-15	23.68792470814073	23.37327825527646	24.032787440692218	28.906009595890588
16-17	24.34948558099496	26.565249989586658	24.979326048623356	24.105938380795024
18-19	24.38736757584512	25.91518829207563	26.098822191493177	23.598621940586074
20-21	24.664253889201422	25.26246868563235	26.075142820172925	23.998134604993304
22-23	24.60326419990277	25.027740436440276	25.836021284770666	24.532974078886287
24-25	24.405433945706363	25.240312617809664	25.842095652940632	24.51215778354334
26-27	24.23457618515478	25.48250942804992	25.82404759098278	24.45886679581252
28-29	24.38092199819167	25.611435706221	25.69424459482853	24.313397700758802
30-31	24.52393886215665	25.43168582193039	25.552546607270056	24.491828708642906
32-33	24.288828628376777	25.522083731632982	25.532967272470835	24.656120367519403
34-35	24.626357233939945	25.45018563798081	25.661379554085716	24.262077573993526
36-37	24.692661635465967	25.280520347268336	25.67165807527701	24.355159941988685
38-39	24.45847177739971	25.484156298168543	25.65657746278668	24.40079446164507
40-41	24.58171972247397	25.30959882322256	25.75176486771873	24.35691658658474
42-43	24.434289616003912	25.279206771468505	25.656110523561214	24.630393088966372
44-45	24.314798723168003	25.39315488541779	25.79690738740102	24.495139004013186
46-47	24.497708156115767	25.340146462148557	25.68578678480368	24.476358596932005
48-49	24.307891877696626	25.441604005283963	25.676619833661206	24.573884283358204
50-51	24.291667634452587	25.54198213590051	25.7814830726941	24.384867156952808
52-53	24.508035110096344	25.319467520518096	25.7587540519808	24.41374331740476
54-55	24.058394932169996	25.210771294629165	26.037555296387243	24.693278476813592
56-57	24.120470632744833	25.409884177832303	25.88793054286338	24.581714646559483
58-59	24.191529122033693	25.23631840449988	25.937838459674456	24.634314013791975
60-61	24.141178113753185	25.275098585600762	26.042652192281952	24.5410711083641
62-63	24.159933192175284	25.29605010141765	26.024757784776494	24.519258921630577
64-65	24.000654284863447	25.204251905792436	25.976444354004276	24.81864945533984
66-67	24.01713659903435	25.2479290679881	25.89872983207502	24.836204500902532
68-69	24.155757637711062	25.184012283479678	25.940255725023626	24.719974353785634
70-71	24.071487665426687	25.078145827617053	25.929937782334267	24.92042872462199
72-73	24.146126967476544	25.214326237642837	25.82973486120795	24.809811933672673
74-75	24.037693772452375	25.349276162349593	25.900615874316635	24.7124141908814
76-77	24.136532623649106	25.088464831300723	25.928359201983525	24.846643343066642
78-79	24.077385435884256	25.154954345168967	25.954976267388762	24.812683951558014
80-81	24.22155264194308	25.28015507527492	25.78271126036083	24.71558102242117
82-83	24.16280286537806	25.28729667831105	25.81956431707576	24.730336139235128
84-85	24.13504146406436	25.32234023833056	25.835781021887655	24.706837275717422
86-87	24.135751899742992	25.391288063035738	25.80270889381363	24.67025114340764
88-89	24.06217049416884	25.192244631806993	25.81942826551539	24.92615660850878
90-91	24.101946350455503	25.22798044174563	25.84180898965669	24.82826421814218
92-93	24.265523561109013	25.1260387405044	25.91436085485827	24.694076843528315
94-95	24.247814550500227	24.948775103399367	25.9802991976401	24.823111148460303
96-97	24.24798699073325	24.8077155070276	25.99219382772239	24.952103674516763
98-99	24.18163639884954	24.83149659848802	25.916229419516846	25.0706375831456
100-101	24.415240643794643	24.748013326491243	25.995284746332487	24.841461283381623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	32.0
2	65.0
3	100.0
4	93.5
5	100.0
6	170.5
7	306.5
8	512.5
9	619.0
10	541.0
11	493.5
12	528.5
13	620.5
14	737.5
15	818.0
16	906.0
17	986.0
18	1107.5
19	1241.0
20	1365.0
21	1506.0
22	1617.5
23	1777.5
24	2020.0
25	2326.0
26	2786.0
27	3565.0
28	4723.0
29	6563.0
30	9636.0
31	14212.5
32	21128.5
33	27102.0
34	32186.5
35	39575.0
36	49393.5
37	59170.5
38	68411.5
39	78394.0
40	87691.0
41	96165.5
42	104559.5
43	114543.0
44	124433.5
45	134542.0
46	143632.5
47	150928.5
48	158020.5
49	162257.0
50	164151.5
51	165772.0
52	166074.0
53	166312.5
54	164952.5
55	157335.0
56	145462.0
57	132008.5
58	119359.5
59	105641.5
60	88748.5
61	73709.0
62	60762.5
63	46824.5
64	33964.0
65	24688.0
66	18344.5
67	13944.5
68	10539.5
69	7831.0
70	5830.0
71	4240.0
72	3071.5
73	2260.0
74	1681.0
75	1203.5
76	828.0
77	556.5
78	359.5
79	253.5
80	184.0
81	137.0
82	120.5
83	100.0
84	82.5
85	77.5
86	73.5
87	58.5
88	50.5
89	42.5
90	35.5
91	33.5
92	27.5
93	25.0
94	22.5
95	16.0
96	12.5
97	11.0
98	10.5
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04986013621034092
2	0.07126445971711869
3	0.06762766804476086
4	0.06685033852700498
5	0.06257502617934768
6	0.009883189582896086
7	0.012048607525216014
8	0.017156772927611745
9	0.005441306624291103
10-11	0.012492795821076512
12-13	0.021765226497164412
14-15	0.0379780992960726
16-17	0.025707397622926335
18-19	0.009563929245246353
20-21	0.01926666733294911
22-23	0.0356877533955419
24-25	0.033133670694344045
26-27	0.020030115966459344
28-29	0.011368444197179627
30-31	0.01142396773416219
32-33	0.00852286292682331
34-35	0.004802785948991637
36-37	5.552353698256228E-4
38-39	0.003886647588779359
40-41	9.855427814404803E-4
42-43	0.22362104519726955
44-45	0.1923335321075957
46-47	0.1986076917866253
48-49	0.20611725016351684
50-51	0.19712243717234174
52-53	0.2049790176553743
54-55	0.20109237006659492
56-57	0.20379914249449485
58-59	0.21284947902265247
60-61	0.21833242829968053
62-63	0.2023138878802113
64-65	0.20298017032400203
66-67	0.19980144783175036
68-69	0.19442954562868744
70-71	0.1864063945347072
72-73	0.19842724029143197
74-75	0.19726124601479814
76-77	0.19050125538717116
78-79	0.20958747122492696
80-81	0.22071994038993067
82-83	0.22002589617764867
84-85	0.21372397473012786
86-87	0.23408723191848257
88-89	0.2263278176251695
90-91	0.2332404979794985
92-93	0.2287708532524022
94-95	0.23221331254532107
96-97	0.23979227534344083
98-99	0.23264361995693597
100-101	0.2396395856167388
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3602076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.257619100472844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70622013506241	42.38603094860299
2	12.200138031534191	13.483011606394179
3	4.27250389031667	7.0826517772922095
4	2.0805033360908802	4.598546435318916
5	1.1697233572582222	3.2318063864150584
6	0.7348238947668133	2.436277132977031
7	0.49600342347329607	1.9185577772772548
8	0.379597153451903	1.6780507933655198
9	0.27949735809466525	1.3899922697865126
>10	1.5685187259371003	15.56105708958527
>50	0.08508188574455576	3.179918281185589
>100	0.02597909542040231	2.2487797430190604
>500	7.552033167426193E-4	0.28822029081628203
>1k	6.545095320960899E-4	0.5170994679640984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.776176849128114E-5	0.0	0.0	0.0
3	0.0	2.776176849128114E-5	0.0	0.0	0.0
4	0.0	2.776176849128114E-5	0.0	0.0	2.776176849128114E-5
5	0.0	2.776176849128114E-5	0.0	0.0	2.776176849128114E-5
6	0.0	2.776176849128114E-5	0.0	0.0	5.552353698256228E-5
7	0.0	2.776176849128114E-5	0.0	0.0	5.552353698256228E-5
8	0.0	2.776176849128114E-5	0.0	0.0	8.328530547384342E-5
9	0.0	2.776176849128114E-5	0.0	0.0	1.9433237943896798E-4
10-11	0.0	2.776176849128114E-5	0.0	0.0	1.9433237943896798E-4
12-13	0.0	2.776176849128114E-5	0.0	2.776176849128114E-5	3.0537945340409253E-4
14-15	0.0	2.776176849128114E-5	0.0	2.776176849128114E-5	3.8866475887793596E-4
16-17	0.0	2.776176849128114E-5	0.0	2.776176849128114E-5	4.164265273692171E-4
18-19	0.0	2.776176849128114E-5	0.0	2.776176849128114E-5	4.997118328430605E-4
20-21	0.0	2.776176849128114E-5	0.0	1.1104707396512456E-4	4.997118328430605E-4
22-23	0.0	2.776176849128114E-5	0.0	2.359750321758897E-4	5.413544855799822E-4
24-25	0.0	2.776176849128114E-5	0.0	3.609029903866548E-4	6.107589068081851E-4
26-27	0.0	2.776176849128114E-5	0.0	8.189721704927936E-4	6.801633280363879E-4
28-29	0.0	2.776176849128114E-5	0.0	0.005954899341379804	6.940442122820284E-4
30-31	0.0	4.164265273692171E-5	0.0	0.0253187328640484	6.940442122820284E-4
32-33	0.0	5.552353698256228E-5	0.0	0.05810538145225143	7.218059807733096E-4
34-35	2.776176849128114E-5	5.552353698256228E-5	0.0	0.10320437436633764	7.218059807733096E-4
36-37	2.776176849128114E-5	5.552353698256228E-5	0.0	0.17766143745995366	7.218059807733096E-4
38-39	4.164265273692171E-5	5.552353698256228E-5	0.0	0.2987305098504307	7.495677492645907E-4
40-41	5.552353698256228E-5	5.552353698256228E-5	0.0	0.43219521187226473	7.495677492645907E-4
42-43	5.552353698256228E-5	5.552353698256228E-5	0.0	0.5710595778656531	7.495677492645907E-4
44-45	5.552353698256228E-5	5.552353698256228E-5	0.0	0.7390876816591321	7.634486335102314E-4
46-47	5.552353698256228E-5	5.552353698256228E-5	0.0	0.9391667471757953	8.606148232297153E-4
48-49	5.552353698256228E-5	5.552353698256228E-5	0.0	1.149267811117811	9.161383602122776E-4
50-51	5.552353698256228E-5	5.552353698256228E-5	0.0	1.3736245431800995	9.439001287035587E-4
52-53	5.552353698256228E-5	5.552353698256228E-5	0.0	1.595080170435049	9.577810129491992E-4
54-55	8.328530547384342E-5	5.552353698256228E-5	0.0	1.849239160972728	9.99423665686121E-4
56-57	8.328530547384342E-5	5.552353698256228E-5	0.0	2.1400992094558804	0.001027185434177402
58-59	8.328530547384342E-5	5.552353698256228E-5	0.0	2.448143792635136	0.001027185434177402
60-61	8.328530547384342E-5	5.552353698256228E-5	0.0	2.7718598941277195	0.0010549472026686834
62-63	1.388088424564057E-4	5.552353698256228E-5	0.0	3.1034603378718275	0.0010549472026686834
64-65	1.388088424564057E-4	6.940442122820285E-5	0.0	3.453897141537269	0.0010827089711599644
66-67	1.388088424564057E-4	8.328530547384342E-5	0.0	3.863563678278859	0.001124351623896886
68-69	1.5268972670204626E-4	8.328530547384342E-5	0.0	4.306710907820934	0.0011382325081425266
70-71	1.6657061094768684E-4	8.328530547384342E-5	0.0	4.766723411721463	0.0012353986978620108
72-73	1.6657061094768684E-4	8.328530547384342E-5	0.0	5.244600613646131	0.0014019693088096975
74-75	1.6657061094768684E-4	9.716618971948399E-5	0.0	5.72912675912446	0.00145749284579226
76-77	1.6657061094768684E-4	1.1104707396512456E-4	0.0	6.269190322469598	0.0014991354985291814
78-79	1.6657061094768684E-4	1.1104707396512456E-4	0.0	6.8673315054984965	0.0015268972670204627
80-81	1.6657061094768684E-4	1.1104707396512456E-4	0.0	7.4876126988991905	0.0015546590355117438
82-83	1.6657061094768684E-4	1.1104707396512456E-4	0.0	8.135600137254183	0.001582420804003025
84-85	1.6657061094768684E-4	1.1104707396512456E-4	0.0	8.794206452057091	0.001582420804003025
86-87	1.6657061094768684E-4	1.2492795821076512E-4	0.0	9.4907075808506	0.001582420804003025
88-89	1.6657061094768684E-4	1.6657061094768684E-4	0.0	10.247090844279798	0.001582420804003025
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2265	0.0	45.478264	1
GTATCAA	4015	0.0	38.779358	1
TCAACGC	4455	0.0	34.095455	4
CAACGCA	4640	0.0	32.734684	5
GTACATG	9160	0.0	31.87073	1
AACGCAG	4975	0.0	30.419395	6
ATCAACG	5055	0.0	29.860298	3
TACATGG	9675	0.0	29.586716	2
ACATGGG	9965	0.0	27.722597	3
TATCAAC	5520	0.0	27.691545	2
ACGCAGA	6035	0.0	24.607885	7
CATGGGG	8615	0.0	24.573883	4
GAGTACA	6805	0.0	23.856695	1
CGCAGAG	6375	0.0	22.627512	8
AGTACAT	6995	0.0	20.970135	2
GTATTAG	785	0.0	20.559929	1
ATGGGGG	4915	0.0	19.990467	5
GTATAGA	1020	0.0	18.150005	1
GCAGAGT	7725	0.0	17.874914	9
ACCGTAT	425	3.6379788E-12	17.863825	8
>>END_MODULE