FastQCFastQC Report
Mon 15 Aug 2016
SRR2921667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3602076
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.040.983691
GTATCAA37500.040.55741
TCAACGC44150.033.5406724
CAACGCA46150.032.1120955
GTACATG87950.031.1810951
AACGCAG50100.029.8589176
ATCAACG50000.029.7125763
TACATGG92750.029.0674152
TATCAAC51500.028.9397242
ACATGGG97550.026.8095953
CATGGGG82350.025.1287444
ACGCAGA61300.023.8604957
CGCAGAG66100.021.9841238
GAGTACA66500.020.2977581
ATGGGGG45950.020.1574155
AGTACAT70100.018.0109082
TAAGGTG17500.017.9139615
AGAGTAC60950.017.29690610-11
GCAGAGT79850.017.2469489
TACCGTA2555.5253768E-616.7606757
Produced by FastQC (version 0.11.5)