##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3602076
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.305700101830165	33.0	31.0	34.0	28.0	34.0
2	31.428899612334664	34.0	31.0	34.0	28.0	34.0
3	31.508229698651554	34.0	31.0	34.0	28.0	34.0
4	34.97888051223794	37.0	35.0	37.0	32.0	37.0
5	34.71000028872239	37.0	35.0	37.0	32.0	37.0
6	34.68855182400371	37.0	35.0	37.0	32.0	37.0
7	34.56590505030988	37.0	35.0	37.0	32.0	37.0
8	34.511607473023886	37.0	35.0	37.0	31.0	37.0
9	36.100691101464825	39.0	37.0	39.0	32.0	39.0
10-11	35.9896044669796	39.0	37.0	39.0	31.0	39.0
12-13	35.90206092264572	39.0	37.0	39.0	31.0	39.0
14-15	37.14611615634984	40.0	38.0	41.0	31.0	41.0
16-17	37.10670360647582	40.0	38.0	41.0	31.0	41.0
18-19	37.05376010944799	40.0	38.0	41.0	31.0	41.0
20-21	36.98825899286967	40.0	38.0	41.0	31.0	41.0
22-23	36.89425639547861	40.0	37.0	41.0	30.0	41.0
24-25	36.81007827153009	40.0	37.0	41.0	30.0	41.0
26-27	36.68164913788604	40.0	37.0	41.0	30.0	41.0
28-29	36.55828458366786	40.0	37.0	41.0	30.0	41.0
30-31	36.408641155822366	40.0	37.0	41.0	29.5	41.0
32-33	36.36548493146729	40.0	36.5	41.0	29.5	41.0
34-35	36.506658798981476	40.0	37.0	41.0	30.0	41.0
36-37	36.50558386330549	40.0	37.0	41.0	30.0	41.0
38-39	36.404945092774284	40.0	37.0	41.0	30.0	41.0
40-41	36.320198407807055	40.0	37.0	41.0	29.0	41.0
42-43	36.19593631561355	40.0	36.0	41.0	29.0	41.0
44-45	36.067505932689926	40.0	36.0	41.0	28.0	41.0
46-47	35.91305277845331	40.0	36.0	41.0	28.0	41.0
48-49	35.76581351975916	40.0	35.0	41.0	27.5	41.0
50-51	35.59805012442825	39.5	35.0	41.0	27.0	41.0
52-53	35.4104796789407	39.0	35.0	41.0	26.5	41.0
54-55	35.18596428837148	39.0	35.0	41.0	26.0	41.0
56-57	34.94724250682107	39.0	35.0	41.0	26.0	41.0
58-59	34.690132440292764	38.0	35.0	40.0	26.0	41.0
60-61	34.39808349407397	38.0	34.0	40.0	25.5	41.0
62-63	34.07858301712679	37.0	34.0	40.0	24.5	41.0
64-65	33.726858067403356	36.5	34.0	39.5	23.5	41.0
66-67	33.37110030438003	36.0	34.0	39.0	23.0	41.0
68-69	33.00298952603998	35.5	33.0	39.0	22.0	40.5
70-71	32.60538908673776	35.0	33.0	38.0	21.5	40.0
72-73	32.2471359571536	35.0	33.0	37.0	20.5	39.5
74-75	31.853708528082137	35.0	33.0	37.0	19.5	39.0
76-77	29.179700261737953	33.0	28.5	34.5	13.0	36.5
78-79	30.751149614833224	34.5	31.5	35.0	15.0	37.0
80-81	30.94116212428611	35.0	32.0	35.0	17.0	37.0
82-83	30.77438246722168	35.0	32.0	35.0	14.0	36.5
84-85	30.58841221006997	35.0	32.0	35.0	11.0	36.0
86-87	30.38953231414329	35.0	32.0	35.0	7.0	36.0
88-89	30.128391377638895	35.0	31.5	35.0	2.0	35.5
90-91	29.93278501064386	34.0	31.0	35.0	2.0	35.0
92-93	29.775167014799244	34.0	31.0	35.0	2.0	35.0
94-95	29.67240308089002	34.0	31.0	35.0	2.0	35.0
96-97	29.464325433444493	34.0	31.0	35.0	2.0	35.0
98-99	29.257378661638455	34.0	31.0	35.0	2.0	35.0
100-101	28.130960868121605	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74282.0
3	24819.0
4	13431.0
5	9698.0
6	8324.0
7	8208.0
8	8487.0
9	9057.0
10	9850.0
11	10672.0
12	11168.0
13	11931.0
14	12796.0
15	13426.0
16	14195.0
17	15010.0
18	14474.0
19	13469.0
20	13088.0
21	12978.0
22	13495.0
23	15622.0
24	18780.0
25	22322.0
26	26710.0
27	32550.0
28	38899.0
29	47391.0
30	57765.0
31	71796.0
32	90503.0
33	120373.0
34	171738.0
35	258311.0
36	463763.0
37	891114.0
38	856858.0
39	94723.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.851819894952534	18.327054157922674	11.62020521767082	27.20092072945397
2	16.088021731978742	20.28083890768212	35.89565985066899	27.735479509670146
3	18.643565214107863	23.2381551562913	28.4893729024004	29.628906727200437
4	13.248506318992515	15.32905403904671	34.2107367345579	37.21170290740288
5	14.291446452615785	36.931232681269265	34.259618447136546	14.517702418978399
6	35.56002035534118	34.55877188331048	15.732080178722782	14.149127582625557
7	30.26074408202381	30.982355730417684	20.0436359477146	18.713264239843912
8	27.884475508012603	34.38308908529415	19.019087881543864	18.713347525149384
9	26.97078767099519	14.447505951430772	18.19198656330032	40.38971981427372
10-11	26.504353405266308	24.607589416840202	27.302416367331716	21.585640810561774
12-13	26.5507905740761	21.819797305213175	27.254275740644395	24.37513638006633
14-15	23.555715593947497	23.50125842833261	23.95659973511302	28.986426242606882
16-17	24.29083950477447	26.82711303148518	24.829959167990904	24.052088295749453
18-19	24.348882619874768	26.139239390569653	25.929565345218148	23.582312644337435
20-21	24.598115886042574	25.39446475764107	25.962805496681142	24.044613859635216
22-23	24.474941672524402	25.141376806041848	25.81328100795208	24.57040051348167
24-25	24.288708408654887	25.433346200982687	25.7881157610937	24.489829629268726
26-27	24.16866938378223	25.610459132008312	25.779005250304586	24.441866233904864
28-29	24.310185356777126	25.744056976730146	25.677355911470407	24.268401755022325
30-31	24.4366958599124	25.547116521422357	25.530720657233978	24.485466961431264
32-33	24.217926927985406	25.646060665628546	25.454373060894824	24.681639345491224
34-35	24.503971903552635	25.581299464855356	25.664259448185618	24.25046918340639
36-37	24.581903145979542	25.43133275299897	25.662971396160383	24.32379270486111
38-39	24.42244755835906	25.615468082220932	25.623505115314764	24.338579244105237
40-41	24.51318430401725	25.462710720724186	25.680287099764058	24.343817875494505
42-43	24.36976644751455	25.422987650481986	25.58363133579139	24.623614566212073
44-45	24.253735797887803	25.47100219026881	25.743612780242103	24.531649231601286
46-47	24.37392350156415	25.48019742606517	25.643858503546408	24.502020568824275
48-49	24.19759523589394	25.58795446790423	25.622701301888767	24.591748994313072
50-51	24.20474338850111	25.63912035096233	25.69052260672041	24.46561365381615
52-53	24.44713060384311	25.42079706474466	25.614883494699548	24.51718883671268
54-55	23.915456435855223	25.439389438439946	25.896186211281176	24.748967914423655
56-57	24.017785502289126	25.544129201706923	25.73804559374315	24.700039702260806
58-59	24.058255715965554	25.397531885709846	25.788393004649407	24.755819393675196
60-61	24.01780659784758	25.4967848118627	25.87272069522804	24.612687895061676
62-63	24.030844267410465	25.46008509385801	25.79989128978492	24.7091793489466
64-65	23.83857743423477	25.363594174272468	25.752065808136255	25.045762583356503
66-67	23.807108403881433	25.49340149803828	25.642585866083245	25.05690423199704
68-69	23.956475547006825	25.375408822937136	25.683982473561045	24.984133156494988
70-71	23.828119628356404	25.27646232676873	25.687601633667224	25.207816411207645
72-73	23.854770269495305	25.44280735118731	25.534632624618474	25.16778975469891
74-75	23.733982433907777	25.57215039742878	25.504240318648364	25.189626850015085
76-77	23.797666855034443	25.308880024195663	25.636422926556325	25.257030194213577
78-79	23.751703557987653	25.342468988645727	25.635237845174537	25.270589608192083
80-81	23.86324207452366	25.477867671346054	25.393290920410067	25.265599333720218
82-83	23.735668587287247	25.460783712056312	25.494953893165214	25.308593807491224
84-85	23.672877869793886	25.44441095878996	25.465465256936042	25.41724591448011
86-87	23.68421418161756	25.54299361553296	25.389590835170072	25.38320136767941
88-89	23.528464291576185	25.389447283401683	25.42594915659373	25.6561392684284
90-91	23.495132871311473	25.37181649938457	25.463480211066	25.669570418237956
92-93	23.532980178547643	25.25357162647625	25.5291384640725	25.68430973090361
94-95	23.429828830762318	25.09483565937402	25.608223986237828	25.86711152362583
96-97	23.386681071541666	24.9892234153631	25.610329528175097	26.01376598492014
98-99	23.21381623969233	25.05386514516313	25.54192042255926	26.190398192585278
100-101	23.182919821102864	25.0161851379901	25.556721551774398	26.24417348913264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	23.0
2	48.0
3	86.5
4	89.5
5	92.0
6	161.5
7	300.5
8	447.0
9	517.5
10	426.5
11	333.0
12	356.5
13	372.5
14	408.0
15	466.0
16	499.0
17	564.0
18	625.0
19	675.5
20	741.0
21	880.0
22	1072.0
23	1271.0
24	1553.0
25	1979.5
26	2589.5
27	3495.0
28	4775.0
29	6689.5
30	9910.0
31	14507.0
32	21703.0
33	27709.0
34	32459.0
35	39558.0
36	49308.5
37	59138.5
38	68040.5
39	77639.0
40	86707.5
41	95656.5
42	104296.0
43	113935.0
44	123718.5
45	133958.5
46	143175.0
47	150806.0
48	158577.0
49	162807.0
50	164335.5
51	166105.0
52	167670.5
53	169409.0
54	167860.5
55	159481.5
56	147461.0
57	133804.5
58	120441.0
59	106206.0
60	89832.0
61	74720.0
62	60741.0
63	46142.0
64	33114.5
65	23676.0
66	17376.0
67	13390.5
68	10082.0
69	7424.5
70	5474.0
71	3816.0
72	2614.5
73	1910.0
74	1424.5
75	945.0
76	596.5
77	351.0
78	176.0
79	98.0
80	69.0
81	50.0
82	33.5
83	22.0
84	15.5
85	12.5
86	10.5
87	7.0
88	7.0
89	5.5
90	3.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16093497194395676
2	3.0537945340409253E-4
3	0.009439001287035587
4	0.006551777363942348
5	0.023458694375132563
6	0.0020266090998635232
7	0.0
8	0.0
9	2.776176849128114E-5
10-11	0.048125025679635856
12-13	2.776176849128114E-4
14-15	0.004511287379833185
16-17	0.0
18-19	0.0015685399197573845
20-21	3.1926033764973307E-4
22-23	0.0
24-25	5.413544855799822E-4
26-27	0.0026234871224260674
28-29	0.00512204628664137
30-31	0.015491066818134875
32-33	0.010618876447915036
34-35	0.009425120402789946
36-37	0.003595149019620907
38-39	1.388088424564057E-5
40-41	0.0034008166401819397
42-43	0.00877271884324484
44-45	0.006524015595451068
46-47	0.0033314122189537364
48-49	0.008578386463805873
50-51	0.002609606238180427
52-53	0.0020127282156178826
54-55	0.0040532181997270465
56-57	0.007384630418680783
58-59	0.015019116753783096
60-61	0.007356868650189501
62-63	0.021057301400636743
64-65	0.03961604363705819
66-67	0.060187514089097505
68-69	0.049998945052797325
70-71	0.06786364307693675
72-73	0.033744429601152225
74-75	0.02749803169061397
76-77	0.2232879039753742
78-79	0.08303544955742188
80-81	0.06626734138868809
82-83	0.02728981842692936
84-85	0.05166465116227419
86-87	0.06668376791605729
88-89	0.29092945290438066
90-91	0.2967039007505672
92-93	0.05165077027802856
94-95	0.008856004148718684
96-97	0.014366715194237989
98-99	6.940442122820285E-4
100-101	1.6657061094768684E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3602076.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.40245748587698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99722776241235	41.34435952389147
2	12.471036622867501	13.56910079360728
3	4.488182954368141	7.325045470915516
4	2.10245683080887	4.575152734158848
5	1.2052197837695564	3.278345902383057
6	0.7715659246180514	2.518504944695094
7	0.550900887318743	2.097925347090287
8	0.37767349293829644	1.6437092914494664
9	0.29848634807396807	1.4614551775087852
>10	1.621543898299618	15.864000983214355
>50	0.08780516164399824	3.2456390342507127
>100	0.026363757744119882	2.2486839522012585
>500	8.707259198624802E-4	0.32991553758885145
>1k	6.658492169126172E-4	0.4981613070449499
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.552353698256228E-5	0.0
3	0.0	0.0	0.0	5.552353698256228E-5	0.0
4	0.0	0.0	0.0	5.552353698256228E-5	0.0
5	0.0	0.0	0.0	5.552353698256228E-5	0.0
6	0.0	0.0	0.0	5.552353698256228E-5	2.776176849128114E-5
7	0.0	0.0	0.0	5.552353698256228E-5	2.776176849128114E-5
8	0.0	0.0	0.0	5.552353698256228E-5	2.776176849128114E-5
9	0.0	0.0	0.0	5.552353698256228E-5	5.552353698256228E-5
10-11	0.0	0.0	0.0	5.552353698256228E-5	5.552353698256228E-5
12-13	0.0	0.0	0.0	8.328530547384342E-5	1.6657061094768684E-4
14-15	0.0	0.0	0.0	8.328530547384342E-5	3.3314122189537367E-4
16-17	0.0	0.0	0.0	8.328530547384342E-5	4.4418829586049825E-4
18-19	0.0	0.0	0.0	8.328530547384342E-5	4.4418829586049825E-4
20-21	0.0	0.0	0.0	1.388088424564057E-4	4.997118328430605E-4
22-23	2.776176849128114E-5	0.0	0.0	2.637368006671708E-4	5.135927170887011E-4
24-25	2.776176849128114E-5	0.0	0.0	3.8866475887793596E-4	5.413544855799822E-4
26-27	2.776176849128114E-5	0.0	0.0	8.189721704927936E-4	6.107589068081851E-4
28-29	2.776176849128114E-5	0.0	0.0	0.005829971383169039	6.107589068081851E-4
30-31	2.776176849128114E-5	0.0	0.0	0.02536037551678532	6.385206752994661E-4
32-33	2.776176849128114E-5	2.776176849128114E-5	0.0	0.05810538145225142	7.218059807733096E-4
34-35	2.776176849128114E-5	2.776176849128114E-5	0.0	0.10330154055605711	7.495677492645907E-4
36-37	5.552353698256228E-5	2.776176849128114E-5	0.0	0.17800845956609465	7.495677492645907E-4
38-39	8.328530547384342E-5	2.776176849128114E-5	0.0	0.3004656203811358	7.634486335102314E-4
40-41	8.328530547384342E-5	2.776176849128114E-5	0.0	0.43423570185637395	8.05091286247153E-4
42-43	8.328530547384342E-5	2.776176849128114E-5	0.0	0.5734332090716576	8.189721704927936E-4
44-45	8.328530547384342E-5	2.776176849128114E-5	0.0	0.7414751937493823	8.74495707475356E-4
46-47	8.328530547384342E-5	2.776176849128114E-5	0.0	0.9408185724010265	9.439001287035587E-4
48-49	8.328530547384342E-5	2.776176849128114E-5	0.0	1.1501006641725493	9.716618971948399E-4
50-51	8.328530547384342E-5	2.776176849128114E-5	0.0	1.3744712771190835	0.0010133045499317616
52-53	8.328530547384342E-5	2.776176849128114E-5	0.0	1.5967181147760348	0.0012076369293707295
54-55	8.328530547384342E-5	2.776176849128114E-5	0.0	1.8503357508281335	0.0014019693088096975
56-57	8.328530547384342E-5	2.776176849128114E-5	0.0	2.1409459433948648	0.0014436119615466193
58-59	8.328530547384342E-5	5.552353698256228E-5	0.0	2.4489905265741196	0.0014436119615466193
60-61	8.328530547384342E-5	5.552353698256228E-5	0.0	2.7719570603174395	0.0014436119615466193
62-63	8.328530547384342E-5	5.552353698256228E-5	0.0	3.1031271966499316	0.0014436119615466193
64-65	1.1104707396512455E-4	5.552353698256228E-5	0.0	3.4524118869229854	0.0014436119615466193
66-67	1.5268972670204626E-4	5.552353698256228E-5	0.0	3.8609957146934155	0.0014852546142835408
68-69	1.6657061094768684E-4	5.552353698256228E-5	0.0	4.303726517708122	0.0014991354985291814
70-71	1.6657061094768684E-4	5.552353698256228E-5	0.0	4.763378118618263	0.0015268972670204627
72-73	1.6657061094768684E-4	5.552353698256228E-5	0.0	5.24113039258472	0.0016240634567399465
74-75	1.6657061094768684E-4	5.552353698256228E-5	0.0	5.725323396841155	0.001637944340985587
76-77	1.6657061094768684E-4	5.552353698256228E-5	0.0	6.266552954462926	0.0016657061094768684
78-79	1.6657061094768684E-4	5.552353698256228E-5	0.0	6.866415367138284	0.0016657061094768684
80-81	1.6657061094768684E-4	5.552353698256228E-5	0.0	7.488681526986104	0.001679586993722509
82-83	1.6657061094768684E-4	5.552353698256228E-5	0.0	8.138390194987558	0.001776753183441993
84-85	1.6657061094768684E-4	5.552353698256228E-5	0.0	8.800147470514226	0.001776753183441993
86-87	1.6657061094768684E-4	5.552353698256228E-5	0.0	9.499952249758195	0.0018183958361789147
88-89	1.6657061094768684E-4	5.552353698256228E-5	0.0	10.26023604166042	0.0018322767204245552
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2215	0.0	40.98369	1
GTATCAA	3750	0.0	40.5574	1
TCAACGC	4415	0.0	33.540672	4
CAACGCA	4615	0.0	32.112095	5
GTACATG	8795	0.0	31.181095	1
AACGCAG	5010	0.0	29.858917	6
ATCAACG	5000	0.0	29.712576	3
TACATGG	9275	0.0	29.067415	2
TATCAAC	5150	0.0	28.939724	2
ACATGGG	9755	0.0	26.809595	3
CATGGGG	8235	0.0	25.128744	4
ACGCAGA	6130	0.0	23.860495	7
CGCAGAG	6610	0.0	21.984123	8
GAGTACA	6650	0.0	20.297758	1
ATGGGGG	4595	0.0	20.157415	5
AGTACAT	7010	0.0	18.010908	2
TAAGGTG	1750	0.0	17.913961	5
AGAGTAC	6095	0.0	17.296906	10-11
GCAGAGT	7985	0.0	17.246948	9
TACCGTA	255	5.5253768E-6	16.760675	7
>>END_MODULE