FastQCFastQC Report
Mon 15 Aug 2016
SRR2921666_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921666_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950875
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.035.931481
GTATCAA27900.034.7045251
TCAACGC27950.033.79684
CAACGCA29350.031.8590625
GTACATG70500.030.1612241
AACGCAG31450.029.8639286
ATCAACG32050.029.175643
GAGTACA47000.028.8820691
TACATGG72900.028.193562
ACATGGG75100.026.6719473
TATCAAC35650.026.3630242
CATGGGG64300.024.2140354
AGTACAT52500.024.1402362
ACGCAGA40100.023.070217
CGCAGAG41950.021.6011778
ATGGGGG37400.020.0521225
TACACTG28350.018.9190885
GTGTAGC17450.018.2238241
GTGTACA28600.017.9232941
GTATAGA8950.017.5005231
Produced by FastQC (version 0.11.5)