##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921666_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2950875
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.385678146312536	31.0	31.0	34.0	26.0	34.0
2	30.419336976320583	31.0	31.0	34.0	26.0	34.0
3	30.490320667598592	33.0	31.0	34.0	26.0	34.0
4	33.93457228788071	37.0	35.0	37.0	30.0	37.0
5	33.93354852374296	37.0	35.0	37.0	30.0	37.0
6	33.988843309187956	37.0	35.0	37.0	30.0	37.0
7	33.89344652009997	37.0	35.0	37.0	30.0	37.0
8	33.865466005845725	37.0	35.0	37.0	29.0	37.0
9	35.42504036938196	39.0	37.0	39.0	30.0	39.0
10-11	35.330767484220786	39.0	36.0	39.0	29.0	39.0
12-13	35.213678315753796	39.0	35.0	39.0	29.0	39.0
14-15	36.37132867369848	40.0	37.0	41.0	28.0	41.0
16-17	36.31059804295336	40.0	37.0	41.0	28.0	41.0
18-19	36.265108315330195	40.0	37.0	41.0	27.0	41.0
20-21	36.18480687931546	40.0	37.0	41.0	27.0	41.0
22-23	36.087022323886984	40.0	36.5	41.0	27.0	41.0
24-25	35.98171220400728	40.0	36.0	41.0	27.0	41.0
26-27	35.84025721184395	40.0	36.0	41.0	27.0	41.0
28-29	35.70946312534417	40.0	36.0	41.0	26.5	41.0
30-31	35.56572372601347	40.0	36.0	41.0	25.5	41.0
32-33	35.39723234633795	39.5	35.5	41.0	25.0	41.0
34-35	35.23274537213538	39.0	35.0	41.0	25.0	41.0
36-37	35.21120243995425	39.0	35.0	41.0	24.5	41.0
38-39	35.40040479518787	40.0	35.0	41.0	25.0	41.0
40-41	35.46284796882281	40.0	35.0	41.0	25.0	41.0
42-43	35.30506832719109	40.0	35.0	41.0	24.0	41.0
44-45	35.192194179692464	40.0	35.0	41.0	24.0	41.0
46-47	35.0399178209853	40.0	35.0	41.0	23.0	41.0
48-49	34.878659041809634	39.5	35.0	41.0	23.0	41.0
50-51	33.180511712627606	37.0	32.5	39.5	18.5	40.0
52-53	33.295544033549376	37.5	33.5	39.5	19.0	40.0
54-55	34.20778870673953	39.0	34.0	40.5	20.0	41.0
56-57	34.22851594865929	39.0	34.0	41.0	20.0	41.0
58-59	34.03495522514508	38.0	34.0	41.0	19.5	41.0
60-61	33.77950472317533	38.0	34.0	40.0	18.0	41.0
62-63	33.48826144787563	37.0	34.0	40.0	17.5	41.0
64-65	33.19469784385987	37.0	33.5	40.0	14.0	41.0
66-67	32.85500995467446	36.0	33.0	39.0	12.5	41.0
68-69	32.49729741178464	36.0	33.0	39.0	9.0	41.0
70-71	32.093693057144066	35.0	32.5	39.0	7.0	40.5
72-73	31.740621849451436	35.0	32.0	37.5	4.0	40.0
74-75	31.353024611344093	35.0	32.0	37.0	2.0	39.0
76-77	30.988668106917437	35.0	32.0	36.5	2.0	39.0
78-79	30.657537679501843	35.0	32.0	36.0	2.0	38.5
80-81	30.35268843986953	35.0	31.0	36.0	2.0	37.0
82-83	30.04874232219257	35.0	31.0	35.0	2.0	37.0
84-85	29.793295039606896	35.0	31.0	35.0	2.0	36.5
86-87	29.56753301986699	35.0	31.0	35.0	2.0	36.0
88-89	29.354060236370568	35.0	31.0	35.0	2.0	36.0
90-91	29.13879001990935	35.0	30.5	35.0	2.0	35.5
92-93	28.949659846655653	34.0	30.0	35.0	2.0	35.0
94-95	28.72596026602279	34.0	30.0	35.0	2.0	35.0
96-97	28.49653407887491	34.0	30.0	35.0	2.0	35.0
98-99	28.235788876180795	34.0	30.0	35.0	2.0	35.0
100-101	27.17860414283899	33.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102518.0
3	17786.0
4	12049.0
5	9258.0
6	8196.0
7	8330.0
8	9048.0
9	9495.0
10	10388.0
11	10909.0
12	10971.0
13	10916.0
14	11245.0
15	11985.0
16	11957.0
17	12198.0
18	12030.0
19	11690.0
20	11797.0
21	12503.0
22	13526.0
23	15820.0
24	18419.0
25	21857.0
26	25957.0
27	31146.0
28	36380.0
29	43592.0
30	52298.0
31	63753.0
32	79328.0
33	102552.0
34	142790.0
35	207418.0
36	352748.0
37	639069.0
38	670717.0
39	118220.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.76865593202524	16.5565399599231	10.693954220111008	25.98084988794065
2	17.062286262322345	18.68604826249327	37.342286859193926	26.90937861599046
3	20.73279786763247	21.563634452249786	28.26397543436645	29.43959224575129
4	13.35069407941812	13.84676061229016	35.396490366583265	37.40605494170845
5	14.305978211846826	36.78824473319583	34.44482800950307	14.460949045454269
6	36.02204327314137	33.99283525839163	15.780834824575674	14.20428664389133
7	30.908149919114607	30.57965999326887	19.46895936275937	19.04323072485715
8	28.326974224510153	34.05152793166667	18.707710683540082	18.913787160283096
9	26.884044292058086	14.62838993088728	18.033088565476447	40.45447721157818
10-11	26.787003414338866	24.52896087372369	27.042434870316807	21.641600841620633
12-13	26.740748750252525	21.589752002917773	27.170347326075962	24.49915192075374
14-15	23.376091955192017	23.46210077474046	23.900196545577977	29.261610724489547
16-17	24.259145545178544	26.7918936467827	24.898031613970783	24.050929194067976
18-19	24.31604369891214	26.115654890573094	26.010928795066963	23.55737261544781
20-21	24.385107959464104	25.4301464880889	26.303760260911357	23.880985291535634
22-23	24.21660867911441	25.32884756138697	26.027986579297174	24.426557180201446
24-25	24.122121438017373	25.53113411553809	26.05234405483366	24.294400391610875
26-27	23.9833824195856	25.73470917200548	25.959791868415174	24.322116539993743
28-29	24.150657550798034	25.941029288780925	25.778499304786507	24.129813855634534
30-31	24.235498907141423	25.732455883185295	25.713103355714708	24.318941853958577
32-33	23.948665477984697	25.763342932488133	25.789235909763654	24.49875567976352
34-35	24.3235242435267	25.767716338630407	25.890720403237793	24.0180390146051
36-37	24.359523527119304	25.550434620533068	25.910076757544438	24.179965094803194
38-39	24.15949010989905	25.758081791650845	25.91988792015328	24.162540178296833
40-41	24.363061426023965	25.570160842663466	26.021863649395392	24.044914081917177
42-43	24.145710795821863	25.534048029468607	25.912764188143576	24.40747698656595
44-45	24.09772852049445	25.724681106360354	26.085073614868087	24.09251675827711
46-47	24.267056376987693	25.628778848617202	25.914202723954265	24.189962050440844
48-49	24.106401864652423	25.76642878584342	25.844343357922806	24.282825991581355
50-51	24.126822709315533	25.848000176562362	25.951509862875554	24.073667251246547
52-53	24.359761237636192	25.625408221875073	25.928742436381906	24.086088104106828
54-55	23.7782154980362	25.63081122029217	26.304074929619357	24.28689835205227
56-57	23.918707907966848	25.83296108725397	26.065290179613616	24.183040825165566
58-59	23.995914721943006	25.604386834741504	26.152095954793666	24.24760248852183
60-61	23.872658101248305	25.734307888103363	26.29913986400495	24.09389414664338
62-63	23.95462684053418	25.66776717563084	26.23523727156629	24.142368712268688
64-65	23.713710797406605	25.62544524285387	26.260126409921558	24.400717549817962
66-67	23.759051338412206	25.709884177841964	26.10605398741051	24.42501049633532
68-69	24.01491296751145	25.499181385987203	26.23795421873122	24.247951427770133
70-71	23.855130322921905	25.441382397531637	26.17502652835313	24.52846075119333
72-73	23.868180292542206	25.64661769402096	26.10578505718031	24.379416956256524
74-75	23.803313422016082	25.7463433433187	26.157817491004252	24.29252574366096
76-77	23.944960529547963	25.44558635618151	26.20122978795571	24.40822332631482
78-79	23.864077050081058	25.549394760773787	26.193176685844065	24.39335150330109
80-81	23.983266232869767	25.702270921496616	25.980351951822833	24.334110893810777
82-83	23.957129643670218	25.741741694827873	26.028614991549514	24.272513669952392
84-85	23.89049111239968	25.74421607689088	26.06830764162981	24.296985169079623
86-87	23.899488028118544	25.867979662423956	26.013456712001297	24.219075597456207
88-89	23.836507027309565	25.653114481387902	26.008152266666713	24.502226224635823
90-91	23.833919443991096	25.733770520312397	26.015551986489704	24.416758049206802
92-93	23.987006677180833	25.600048772510377	26.170391655825032	24.242552894483765
94-95	23.998948424572898	25.37180459989525	26.24998556461381	24.379261410918044
96-97	23.988545382070544	25.210621523927507	26.274679509661375	24.526153584340577
98-99	23.90305023769056	25.26275710184623	26.176140323704793	24.658052336758416
100-101	24.118042284513184	25.175460824562272	26.25266562543946	24.453831265485086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	19.5
2	43.0
3	74.0
4	71.5
5	73.0
6	128.0
7	240.0
8	384.0
9	435.0
10	392.0
11	366.5
12	407.5
13	492.0
14	549.5
15	629.5
16	712.5
17	812.5
18	905.0
19	983.5
20	1084.5
21	1215.0
22	1358.5
23	1539.0
24	1811.0
25	2200.5
26	2760.0
27	3645.5
28	4992.0
29	7101.5
30	10283.5
31	14794.0
32	22525.0
33	27698.5
34	30947.0
35	37066.0
36	45273.0
37	53901.5
38	61688.0
39	69486.5
40	76316.5
41	83009.0
42	89425.5
43	96438.5
44	103160.0
45	111483.5
46	118310.0
47	122125.5
48	126605.5
49	129918.0
50	130667.5
51	130623.0
52	130901.0
53	130715.5
54	129036.0
55	122873.0
56	113581.5
57	103447.0
58	94344.5
59	83281.0
60	69278.0
61	57626.5
62	47380.5
63	36341.0
64	26402.5
65	19267.0
66	14409.0
67	11142.5
68	8573.0
69	6451.5
70	4921.0
71	3643.0
72	2579.5
73	1888.5
74	1453.0
75	1110.5
76	845.5
77	579.5
78	366.0
79	253.0
80	179.0
81	133.0
82	111.0
83	85.5
84	74.5
85	66.0
86	56.5
87	48.5
88	43.0
89	41.5
90	37.0
91	25.5
92	19.5
93	17.0
94	11.0
95	8.0
96	9.5
97	12.5
98	12.0
99	8.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05235735163299022
2	0.07343584530012284
3	0.06913203710763756
4	0.07038590248655059
5	0.065505993984835
6	0.01067479984750286
7	0.013690854407590968
8	0.017418562290845936
9	0.0063709916550175796
10-11	0.012775871563519295
12-13	0.023010124115728385
14-15	0.03990341847757021
16-17	0.027737535476765367
18-19	0.010878129368407676
20-21	0.020366840343965773
22-23	0.037412631846486216
24-25	0.03415935951200915
26-27	0.021214046681069177
28-29	0.011606726818316601
30-31	0.012064218240352437
32-33	0.009082051933748465
34-35	0.0054051764307197015
36-37	8.472063371034016E-4
38-39	0.004117422798322531
40-41	0.0013894183928495785
42-43	0.21869784385987207
44-45	0.19024865505993985
46-47	0.19592493751853265
48-49	0.20275342059558604
50-51	0.19451857499894099
52-53	0.201245393315542
54-55	0.19716185877070358
56-57	0.20043207523192272
58-59	0.2085822001948575
60-61	0.2129029525140848
62-63	0.1990596009658152
64-65	0.19834794764264835
66-67	0.1965349260812471
68-69	0.19157029694582117
70-71	0.18440293133392638
72-73	0.19524717244884993
74-75	0.19426441309780998
76-77	0.18877451603337994
78-79	0.20634557546490448
80-81	0.21720676070657008
82-83	0.21585123056720462
84-85	0.2095141271656712
86-87	0.22935569958063284
88-89	0.2216461219129919
90-91	0.22872876689117635
92-93	0.2234421993476511
94-95	0.2277629516668785
96-97	0.23415088744863813
98-99	0.2274240691320371
100-101	0.2342694963358326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2950875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.835214206647656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05526691398596	45.923983488414216
2	12.099138279551306	14.23710784786503
3	3.9280563643310105	6.933241128336018
4	1.8846293423529856	4.4353028422988565
5	1.0320151850414603	3.035941723821372
6	0.6445987256251534	2.2755062459692796
7	0.4356287571929681	1.7941217870816786
8	0.32948213944174415	1.5508121801055657
9	0.2405344165368368	1.2736704528914227
>10	1.2680565614174883	13.161019546016812
>50	0.06233164007114772	2.4903944004726033
>100	0.018526953219458697	1.7755984764727013
>500	0.0010408327397601993	0.43968609141654036
>1k	6.938884928334036E-4	0.6736137888377897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.3888253484136064E-5
5	0.0	0.0	0.0	0.0	3.3888253484136064E-5
6	0.0	0.0	0.0	0.0	1.3555301393654426E-4
7	0.0	0.0	0.0	0.0	1.3555301393654426E-4
8	0.0	0.0	0.0	0.0	1.3555301393654426E-4
9	0.0	0.0	0.0	0.0	1.3555301393654426E-4
10-11	0.0	0.0	0.0	0.0	1.3555301393654426E-4
12-13	0.0	0.0	0.0	0.0	3.558266615834286E-4
14-15	0.0	0.0	3.3888253484136064E-5	3.3888253484136064E-5	4.405472952937688E-4
16-17	0.0	0.0	3.3888253484136064E-5	3.3888253484136064E-5	4.405472952937688E-4
18-19	0.0	0.0	3.3888253484136064E-5	6.777650696827213E-5	5.42212055746177E-4
20-21	0.0	0.0	3.3888253484136064E-5	1.3555301393654426E-4	6.269326894565172E-4
22-23	0.0	0.0	3.3888253484136064E-5	1.3555301393654426E-4	8.810945905875376E-4
24-25	3.3888253484136064E-5	0.0	3.3888253484136064E-5	2.3721777438895242E-4	9.488710975558097E-4
26-27	3.3888253484136064E-5	0.0	3.3888253484136064E-5	3.727707883254967E-4	0.001084424111492354
28-29	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.0035752107425763542	0.001084424111492354
30-31	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.014910831533019867	0.001084424111492354
32-33	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.034904901088660144	0.001084424111492354
34-35	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.05982971152624222	0.0011522006184606262
36-37	3.3888253484136064E-5	1.6944126742068032E-5	3.3888253484136064E-5	0.10180031346634473	0.0012199771254288983
38-39	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.17825221332655566	0.0012369212521709662
40-41	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.2664463930190198	0.0013046977591392385
42-43	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.3543186343033846	0.0013216418858813064
44-45	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.4610496886516711	0.0013555301393654426
46-47	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.5842673783199899	0.0014063625195916466
48-49	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.7205828779599271	0.0014233066463337147
50-51	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	0.8757402465370441	0.0014910831533019866
52-53	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	1.0314398271699072	0.0015758037870123268
54-55	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	1.2006269326894565	0.001660524420722667
56-57	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	1.4009403990341847	0.001660524420722667
58-59	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	1.6162833057991275	0.001660524420722667
60-61	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	1.8470284237726098	0.001660524420722667
62-63	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	2.085245901639344	0.0017283009276909391
64-65	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	2.3311051806667513	0.0017283009276909391
66-67	3.3888253484136064E-5	3.3888253484136064E-5	3.3888253484136064E-5	2.615834286440463	0.001745245054433007
68-69	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	2.937942135807176	0.001762189181175075
70-71	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	3.2819417969246407	0.0018299656881433472
72-73	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	3.638734273732368	0.002219680603210912
74-75	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	3.9913415512348034	0.002270512983437116
76-77	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	4.380937857415173	0.002270512983437116
78-79	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	4.820129622569577	0.0023044012369212523
80-81	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	5.292260770110561	0.00232134536366332
82-83	6.777650696827213E-5	3.3888253484136064E-5	3.3888253484136064E-5	5.789045622061253	0.0024399542508577966
84-85	1.0166476045240818E-4	3.3888253484136064E-5	3.3888253484136064E-5	6.2841868937179655	0.002490786631084
86-87	1.0166476045240818E-4	3.3888253484136064E-5	3.3888253484136064E-5	6.797407548608463	0.002626339645020545
88-89	1.0166476045240818E-4	3.3888253484136064E-5	3.3888253484136064E-5	7.368966831871902	0.002728004405472953
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1955	0.0	35.93148	1
GTATCAA	2790	0.0	34.704525	1
TCAACGC	2795	0.0	33.7968	4
CAACGCA	2935	0.0	31.859062	5
GTACATG	7050	0.0	30.161224	1
AACGCAG	3145	0.0	29.863928	6
ATCAACG	3205	0.0	29.17564	3
GAGTACA	4700	0.0	28.882069	1
TACATGG	7290	0.0	28.19356	2
ACATGGG	7510	0.0	26.671947	3
TATCAAC	3565	0.0	26.363024	2
CATGGGG	6430	0.0	24.214035	4
AGTACAT	5250	0.0	24.140236	2
ACGCAGA	4010	0.0	23.07021	7
CGCAGAG	4195	0.0	21.601177	8
ATGGGGG	3740	0.0	20.052122	5
TACACTG	2835	0.0	18.919088	5
GTGTAGC	1745	0.0	18.223824	1
GTGTACA	2860	0.0	17.923294	1
GTATAGA	895	0.0	17.500523	1
>>END_MODULE