FastQCFastQC Report
Mon 15 Aug 2016
SRR2921666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950875
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27550.037.6081281
GGTATCA16450.037.270961
TCAACGC29300.033.85034
CAACGCA30750.032.437025
ATCAACG32650.030.522493
GTACATG67350.030.0620651
AACGCAG34250.029.117356
TATCAAC34400.028.6938442
TACATGG69000.027.8541432
ACATGGG73550.025.8084053
CATGGGG61050.023.0863154
GAGTACA47600.022.9650651
ACGCAGA44650.021.909457
CGTTATA1100.002894225521.5705472
TATACCG2509.511496E-920.898715
CGCAGAG46950.020.6338528
GTATAGG7650.019.259591
AGTACAT53300.019.2313792
CCCTATA4700.019.184022
ATGGGGG34400.018.363695
Produced by FastQC (version 0.11.5)