##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2950875
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385733384165714	33.0	31.0	34.0	29.0	34.0
2	31.51009344685898	34.0	31.0	34.0	28.0	34.0
3	31.592038293726436	34.0	31.0	34.0	28.0	34.0
4	35.06323446435379	37.0	35.0	37.0	32.0	37.0
5	34.810920489685266	37.0	35.0	37.0	32.0	37.0
6	34.79168416147753	37.0	35.0	37.0	32.0	37.0
7	34.67266217647308	37.0	35.0	37.0	32.0	37.0
8	34.61912449697124	37.0	35.0	37.0	32.0	37.0
9	36.21490405388232	39.0	37.0	39.0	32.0	39.0
10-11	36.1113852670818	39.0	37.0	39.0	32.0	39.0
12-13	36.02489041386029	39.0	37.0	39.0	31.0	39.0
14-15	37.286055322573816	40.0	38.0	41.0	31.0	41.0
16-17	37.25142339136697	40.0	38.0	41.0	31.0	41.0
18-19	37.20010285084932	40.0	38.0	41.0	31.0	41.0
20-21	37.13724319057907	40.0	38.0	41.0	31.0	41.0
22-23	37.05180073706951	40.0	38.0	41.0	31.0	41.0
24-25	36.967951878680054	40.0	37.0	41.0	30.5	41.0
26-27	36.84319735671623	40.0	37.0	41.0	30.0	41.0
28-29	36.7245466175287	40.0	37.0	41.0	30.0	41.0
30-31	36.576753844198755	40.0	37.0	41.0	30.0	41.0
32-33	36.53484542720379	40.0	37.0	41.0	30.0	41.0
34-35	36.6771691447452	40.0	37.0	41.0	30.0	41.0
36-37	36.676123183801415	40.0	37.0	41.0	30.0	41.0
38-39	36.57342093446859	40.0	37.0	41.0	30.0	41.0
40-41	36.49060295675012	40.0	37.0	41.0	30.0	41.0
42-43	36.37172736900072	40.0	37.0	41.0	29.5	41.0
44-45	36.250634811708395	40.0	36.0	41.0	29.0	41.0
46-47	36.101403143135514	40.0	36.0	41.0	28.5	41.0
48-49	35.959708899902566	40.0	36.0	41.0	28.0	41.0
50-51	35.79500232981743	40.0	35.0	41.0	28.0	41.0
52-53	35.619313932308216	39.0	35.0	41.0	27.5	41.0
54-55	35.406751387300375	39.0	35.0	41.0	27.0	41.0
56-57	35.17688194179692	39.0	35.0	41.0	26.5	41.0
58-59	34.92932723344771	38.5	35.0	40.5	26.0	41.0
60-61	34.64884212309909	38.0	34.5	40.0	26.0	41.0
62-63	34.33841504638455	37.5	34.0	40.0	26.0	41.0
64-65	33.99616961070869	37.0	34.0	40.0	25.5	41.0
66-67	33.64000982759351	36.0	34.0	39.0	24.5	41.0
68-69	33.277725589867416	36.0	34.0	39.0	23.5	41.0
70-71	32.88582691574533	35.0	33.0	38.5	23.0	40.0
72-73	32.52868013724743	35.0	33.0	37.5	22.0	39.5
74-75	32.13199406955564	35.0	33.0	37.0	21.5	39.0
76-77	29.45347413902656	33.0	29.0	34.5	16.5	36.5
78-79	31.023300715889356	34.5	31.5	36.0	18.5	37.0
80-81	31.207476595924938	35.0	32.0	35.5	20.0	37.0
82-83	31.040593552759773	35.0	32.0	35.0	18.5	37.0
84-85	30.84761909603084	35.0	32.0	35.0	18.0	36.0
86-87	30.645020544753674	35.0	32.0	35.0	16.0	36.0
88-89	30.38379955098064	35.0	32.0	35.0	10.0	36.0
90-91	30.192870589232008	34.5	32.0	35.0	4.5	35.0
92-93	30.03941068327191	34.0	31.0	35.0	2.0	35.0
94-95	29.93771457618503	34.0	31.0	35.0	2.0	35.0
96-97	29.741412631846487	34.0	31.0	35.0	2.0	35.0
98-99	29.542367772270936	34.0	31.0	35.0	2.0	35.0
100-101	28.432432752996995	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54917.0
3	18929.0
4	10314.0
5	7225.0
6	6531.0
7	6370.0
8	6663.0
9	7182.0
10	7683.0
11	8377.0
12	8910.0
13	9529.0
14	10031.0
15	10703.0
16	11247.0
17	11771.0
18	11414.0
19	10517.0
20	10271.0
21	10120.0
22	10992.0
23	12501.0
24	14801.0
25	17829.0
26	21036.0
27	25856.0
28	30902.0
29	37706.0
30	45749.0
31	57567.0
32	73262.0
33	95820.0
34	137416.0
35	208222.0
36	373065.0
37	724166.0
38	735645.0
39	89633.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38813620128862	18.130224213298433	11.19786902500359	27.28377056040936
2	16.163379724203182	19.984018261745355	35.7301987076345	28.12240330641696
3	19.216143614479392	22.629082013417086	28.190557337134635	29.964217034968883
4	13.057781498194487	15.002177460487989	34.18989149977039	37.75014954154713
5	13.987028997813308	37.054640846691264	34.55260618300482	14.4057239724906
6	36.11639588372883	34.52243541299961	15.480309581113898	13.88085912215766
7	30.667253159572383	30.69534653123109	19.53563588279269	19.10176442640384
8	28.27334265260304	34.557377049180324	18.442089210827298	18.727191087389333
9	26.552244030834267	14.578262093963378	17.964887001236583	40.90460687396577
10-11	26.70150278434663	24.781447860248683	26.996296013764926	21.520753341639757
12-13	26.615507708240422	21.718440133547144	27.18659391067306	24.47945824753937
14-15	23.20003741449568	23.657687187704187	23.850131221854404	29.292144175945733
16-17	24.19500995467446	27.12736052865675	24.74291523700597	23.93471427966281
18-19	24.196974965111654	26.340380135568086	25.943002031284628	23.519642868035632
20-21	24.291974676582175	25.531017933552036	26.269225079896053	23.907782309969736
22-23	24.145634769348074	25.43759054517728	25.976498496208748	24.440276189265898
24-25	23.9398433203055	25.692484329150034	26.09446869913561	24.27320365140886
26-27	23.94102052027441	25.880603458939287	25.940333246463908	24.238042774322395
28-29	24.051074772761048	26.058958682086093	25.79869783154367	24.09126871360919
30-31	24.162498279883756	25.844721124247638	25.745869517913274	24.24691107795533
32-33	23.878881914425072	25.905775089895005	25.741838191196788	24.473504804483134
34-35	24.205276842130413	25.924553938109955	25.866243200681634	24.003926019077998
36-37	24.267685792042776	25.70390632532473	25.923391743600092	24.105016139032397
38-39	24.095819036726397	25.867429152370057	25.92122675477613	24.11552505612742
40-41	24.24821063048164	25.709952690154402	25.983560167550056	24.058276511813904
42-43	24.037354208508184	25.704886230613383	25.87432531083319	24.383434250045244
44-45	24.039619838522707	25.789801617823066	26.023272156189726	24.147306387464507
46-47	24.229819342693094	25.781593000429552	25.82546316575179	24.16312449112557
48-49	24.009232684353325	25.941006325991843	25.808813075422343	24.240947914232493
50-51	24.064668860319106	25.988172983711294	25.872305570057343	24.074852585912264
52-53	24.31168192120417	25.74533620530728	25.83556548425927	24.107416389229282
54-55	23.70759786021591	25.769266613911984	26.216404898390756	24.30673062748135
56-57	23.87480701354832	25.897455840819365	25.946529599050788	24.28120754658152
58-59	23.86041534836002	25.744982356862828	26.060596406161928	24.33400588861522
60-61	23.785222666885147	25.91110947683318	26.13029664912209	24.173371207159583
62-63	23.819944225112934	25.85443110377841	26.06265056973937	24.262974101369288
64-65	23.5985619435087	25.74661178831366	26.130772690080654	24.52405357809698
66-67	23.648697449627836	25.882735710127058	25.987920378393536	24.480646461851567
68-69	23.848834441693604	25.72567252615954	26.033599353360444	24.39189367878641
70-71	23.644819880157485	25.620373227710953	26.045722598825993	24.689084293305573
72-73	23.665976132677443	25.820276071217545	25.941738186258405	24.572009609846603
74-75	23.56172806864113	25.8822169522872	25.97594360536774	24.58011137370393
76-77	23.657263378119524	25.58270727764268	26.06332106128327	24.69670828295453
78-79	23.54109397390132	25.67216395194044	26.122743498647583	24.663998575510654
80-81	23.67123252366886	25.797775929264876	25.844979699326903	24.686011847739355
82-83	23.59067577496371	25.80632750378594	25.937782568644845	24.6652141526055
84-85	23.50185430939605	25.81416354676596	25.917779738287745	24.766202405550246
86-87	23.483281901726066	25.9348570653464	25.85674319237682	24.725117840550716
88-89	23.33925242078441	25.80253088147499	25.86483169857417	24.993384999166434
90-91	23.28466732754136	25.817229115444775	25.932590441291058	24.965513115722807
92-93	23.397408566536974	25.633274750230683	26.08208223345211	24.887234449780234
94-95	23.30652499936453	25.416418833617175	26.14915736763173	25.12789879938656
96-97	23.192388253955343	25.301700763275985	26.199804097016038	25.306106885752634
98-99	23.06563428651599	25.31962813439242	26.170162437886564	25.44457514120503
100-101	23.022790401269862	25.355696007566586	26.208783546680053	25.4127300444835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	21.5
2	33.0
3	61.5
4	61.0
5	49.0
6	112.0
7	232.5
8	335.5
9	377.0
10	313.5
11	235.0
12	243.0
13	282.5
14	321.0
15	349.0
16	376.5
17	427.0
18	468.0
19	523.5
20	626.5
21	776.0
22	969.0
23	1189.5
24	1459.0
25	1943.5
26	2618.5
27	3580.0
28	5093.0
29	7338.5
30	10684.5
31	15284.5
32	23242.0
33	28850.5
34	31652.5
35	37393.0
36	45744.5
37	54131.0
38	61877.5
39	69615.5
40	76373.5
41	83137.0
42	89767.5
43	96783.5
44	103958.5
45	112553.5
46	118922.5
47	122843.5
48	127177.5
49	130146.0
50	131284.5
51	131737.5
52	132342.0
53	132006.5
54	129976.0
55	123565.5
56	113792.5
57	103268.5
58	94046.0
59	83089.5
60	69056.5
61	56886.5
62	46499.0
63	35679.5
64	25518.0
65	18339.0
66	13586.0
67	10510.0
68	8073.5
69	5995.5
70	4419.0
71	3097.0
72	2167.0
73	1647.5
74	1283.0
75	912.0
76	621.5
77	376.5
78	200.0
79	113.0
80	74.5
81	49.5
82	25.5
83	16.0
84	9.5
85	9.0
86	9.5
87	4.5
88	3.5
89	4.0
90	2.5
91	2.5
92	2.5
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15649595458974033
2	2.3721777438895242E-4
3	0.010471470326598044
4	0.006438768161985852
5	0.02561951963400686
6	0.001660524420722667
7	3.388825348413606E-5
8	0.0
9	6.777650696827211E-5
10-11	0.045749142203583684
12-13	2.3721777438895242E-4
14-15	0.004981573262168
16-17	0.0
18-19	0.00111831236497649
20-21	3.2193840809929255E-4
22-23	0.0
24-25	4.7443554877790484E-4
26-27	0.0029143897996357013
28-29	0.0061507180073706955
30-31	0.01670690896767908
32-33	0.011843944592705553
34-35	0.010285084932435294
36-37	0.003710763756512899
38-39	0.0
40-41	0.003897149150675647
42-43	0.008709281145422968
44-45	0.0061507180073706955
46-47	0.004151311051806668
48-49	0.009166772567458806
50-51	0.0029991104333460415
52-53	0.0019994069555640277
54-55	0.003727707883254967
56-57	0.007269030372347185
58-59	0.01477527851908332
60-61	0.006794594823569281
62-63	0.0210954377938747
64-65	0.0406489600542212
66-67	0.06018553818782564
68-69	0.04991739738213241
70-71	0.0679459482356928
72-73	0.033346041428389886
74-75	0.027347820561697798
76-77	0.22078197144914644
78-79	0.08302622103613336
80-81	0.06635320032193841
82-83	0.027703647223281228
84-85	0.05181513957724403
86-87	0.06692930063116873
88-89	0.29477697293175753
90-91	0.2987080103359173
92-93	0.05095098911339857
94-95	0.010166476045240817
96-97	0.014741390265599185
98-99	8.133180836192654E-4
100-101	1.8638539416274835E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2950875.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.21082827572969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52824365968326	45.129832781927504
2	12.365354630846992	14.395950699694662
3	4.068425649321559	7.104792804756939
4	1.956293731489895	4.555099138425788
5	1.025487341093459	2.9847233755662996
6	0.6501154136547136	2.27062540221957
7	0.4465335074207775	1.8195159723881107
8	0.32394880522278513	1.508586261676108
9	0.24380577728005318	1.277292261049198
>10	1.3042542789350993	13.397759780794036
>50	0.0666161052818271	2.630148257594999
>100	0.019286255853278252	1.8591725705606539
>500	8.758092410890095E-4	0.37967682999587804
>1k	7.590346751692612E-4	0.6868238633503199
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3888253484136064E-5
2	0.0	0.0	0.0	3.3888253484136064E-5	3.3888253484136064E-5
3	0.0	0.0	0.0	3.3888253484136064E-5	3.3888253484136064E-5
4	0.0	0.0	0.0	6.777650696827213E-5	3.3888253484136064E-5
5	0.0	0.0	0.0	6.777650696827213E-5	3.3888253484136064E-5
6	0.0	0.0	0.0	6.777650696827213E-5	3.3888253484136064E-5
7	0.0	0.0	0.0	6.777650696827213E-5	3.3888253484136064E-5
8	0.0	0.0	0.0	6.777650696827213E-5	3.3888253484136064E-5
9	0.0	0.0	0.0	6.777650696827213E-5	1.0166476045240818E-4
10-11	0.0	0.0	0.0	1.0166476045240818E-4	1.0166476045240818E-4
12-13	0.0	0.0	0.0	1.0166476045240818E-4	2.541619011310205E-4
14-15	0.0	0.0	0.0	1.0166476045240818E-4	4.0665904180963277E-4
16-17	0.0	0.0	0.0	1.0166476045240818E-4	4.7443554877790484E-4
18-19	3.3888253484136064E-5	0.0	0.0	1.3555301393654426E-4	5.42212055746177E-4
20-21	3.3888253484136064E-5	0.0	0.0	2.0332952090481636E-4	5.761003092303131E-4
22-23	3.3888253484136064E-5	0.0	0.0	2.0332952090481636E-4	6.947091964247893E-4
24-25	3.3888253484136064E-5	0.0	0.0	3.3888253484136064E-4	8.133180836192654E-4
26-27	3.3888253484136064E-5	0.0	0.0	4.744355487779049E-4	8.810945905875376E-4
28-29	3.3888253484136064E-5	0.0	0.0	0.0036599313762866945	8.810945905875376E-4
30-31	3.3888253484136064E-5	0.0	0.0	0.015198881687635023	8.980387173296055E-4
32-33	3.3888253484136064E-5	0.0	0.0	0.03536239251069598	9.827593510399457E-4
34-35	3.3888253484136064E-5	0.0	0.0	0.06032109120176219	9.827593510399457E-4
36-37	3.3888253484136064E-5	0.0	0.0	0.10205447536747575	9.827593510399457E-4
38-39	3.3888253484136064E-5	0.0	0.0	0.17774388952429365	0.001016647604524082
40-41	3.3888253484136064E-5	0.0	1.6944126742068032E-5	0.2656500190621426	0.0010505358580082179
42-43	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.353420595586055	0.0010505358580082179
44-45	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.4605921972296353	0.0010505358580082179
46-47	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.583081289448045	0.001084424111492354
48-49	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.7183631973567162	0.00111831236497649
50-51	3.3888253484136064E-5	0.0	3.3888253484136064E-5	0.872470030075825	0.0011860888719447621
52-53	3.3888253484136064E-5	0.0	3.3888253484136064E-5	1.0282373872156563	0.0012199771254288983
54-55	3.3888253484136064E-5	0.0	3.3888253484136064E-5	1.1966620070318128	0.0012538653789130345
56-57	3.3888253484136064E-5	0.0	3.3888253484136064E-5	1.3958910492650485	0.0013385860126233745
58-59	3.3888253484136064E-5	0.0	3.3888253484136064E-5	1.6100648112847884	0.0013894183928495785
60-61	3.3888253484136064E-5	0.0	3.3888253484136064E-5	1.8405388232303976	0.0013894183928495785
62-63	3.3888253484136064E-5	0.0	3.3888253484136064E-5	2.077214385563604	0.0013894183928495785
64-65	3.3888253484136064E-5	0.0	3.3888253484136064E-5	2.3214300842970306	0.0013894183928495785
66-67	3.3888253484136064E-5	0.0	3.3888253484136064E-5	2.604549498030245	0.0014063625195916466
68-69	3.3888253484136064E-5	0.0	3.3888253484136064E-5	2.925793196933113	0.0014233066463337147
70-71	3.3888253484136064E-5	0.0	3.3888253484136064E-5	3.2681492777565975	0.0014910831533019866
72-73	3.3888253484136064E-5	0.0	3.3888253484136064E-5	3.625856737408396	0.001677468547464735
74-75	3.3888253484136064E-5	0.0	3.3888253484136064E-5	3.979243444740967	0.001711356800948871
76-77	3.3888253484136064E-5	0.0	3.3888253484136064E-5	4.367450332528487	0.0017621891811750753
78-79	3.3888253484136064E-5	0.0	3.3888253484136064E-5	4.807590968780446	0.0017960774346592113
80-81	3.3888253484136064E-5	0.0	3.3888253484136064E-5	5.282873723895454	0.0018807980683695513
82-83	3.3888253484136064E-5	0.0	3.3888253484136064E-5	5.78074299995764	0.0019994069555640277
84-85	3.3888253484136064E-5	0.0	3.3888253484136064E-5	6.278019231583853	0.002033295209048164
86-87	3.3888253484136064E-5	0.0	3.3888253484136064E-5	6.792798746134621	0.0021180158427585036
88-89	3.3888253484136064E-5	0.0	3.3888253484136064E-5	7.366831871902402	0.002168848222984708
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2755	0.0	37.608128	1
GGTATCA	1645	0.0	37.27096	1
TCAACGC	2930	0.0	33.8503	4
CAACGCA	3075	0.0	32.43702	5
ATCAACG	3265	0.0	30.52249	3
GTACATG	6735	0.0	30.062065	1
AACGCAG	3425	0.0	29.11735	6
TATCAAC	3440	0.0	28.693844	2
TACATGG	6900	0.0	27.854143	2
ACATGGG	7355	0.0	25.808405	3
CATGGGG	6105	0.0	23.086315	4
GAGTACA	4760	0.0	22.965065	1
ACGCAGA	4465	0.0	21.90945	7
CGTTATA	110	0.0028942255	21.570547	2
TATACCG	250	9.511496E-9	20.89871	5
CGCAGAG	4695	0.0	20.633852	8
GTATAGG	765	0.0	19.25959	1
AGTACAT	5330	0.0	19.231379	2
CCCTATA	470	0.0	19.18402	2
ATGGGGG	3440	0.0	18.36369	5
>>END_MODULE