FastQCFastQC Report
Mon 15 Aug 2016
SRR2921665_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921665_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777851
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.052.3535271
GTATCAA38900.042.0968741
TCAACGC45700.033.3408784
CAACGCA48150.031.7406965
ATCAACG49500.030.8772643
TATCAAC50750.030.2113632
AACGCAG51350.029.7439446
GTACATG80450.028.438151
TACATGG84150.026.7379472
ACATGGG88500.024.8328113
ACGCAGA61350.024.28337
CATGGGG74350.023.4300524
CGCAGAG64000.022.9088048
ATGGGGG46950.020.4207955
GAGTACA61300.020.3645881
TAAGGCG6850.020.0939035
AGTACAT61150.018.8631252
GCAGAGT75000.018.1579999
GGTATAT4353.6379788E-1217.4586661
TATACTG10650.017.3808865
Produced by FastQC (version 0.11.5)