##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921665_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2777851
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.577907166367094	31.0	31.0	34.0	27.0	34.0
2	30.63623930873182	33.0	31.0	34.0	27.0	34.0
3	30.701251435012175	33.0	31.0	34.0	27.0	34.0
4	34.16877327113657	37.0	35.0	37.0	30.0	37.0
5	34.166305536186066	37.0	35.0	37.0	30.0	37.0
6	34.20958827525306	37.0	35.0	37.0	30.0	37.0
7	34.12456103657107	37.0	35.0	37.0	30.0	37.0
8	34.09501193548538	37.0	35.0	37.0	30.0	37.0
9	35.669995978905995	39.0	37.0	39.0	30.0	39.0
10-11	35.57437745941017	39.0	37.0	39.0	30.0	39.0
12-13	35.465978736800494	39.0	37.0	39.0	30.0	39.0
14-15	36.62536723531967	40.0	37.0	41.0	30.0	41.0
16-17	36.56165971464992	40.0	37.0	41.0	30.0	41.0
18-19	36.525208515503536	40.0	37.0	41.0	29.0	41.0
20-21	36.447535702958874	40.0	37.0	41.0	29.0	41.0
22-23	36.34704057201053	40.0	37.0	41.0	28.5	41.0
24-25	36.23106962900458	40.0	36.5	41.0	28.0	41.0
26-27	36.08267002081825	40.0	36.0	41.0	27.0	41.0
28-29	35.94554999530213	40.0	36.0	41.0	27.0	41.0
30-31	35.79853725055807	40.0	36.0	41.0	27.0	41.0
32-33	35.62500922475684	40.0	35.5	41.0	26.0	41.0
34-35	35.4510403905753	39.0	35.0	41.0	25.5	41.0
36-37	35.41748243516301	39.0	35.0	41.0	25.5	41.0
38-39	35.60585016978953	40.0	35.5	41.0	26.0	41.0
40-41	35.66295528449871	40.0	35.5	41.0	26.0	41.0
42-43	35.5040273938379	40.0	35.0	41.0	25.0	41.0
44-45	35.37868157795361	40.0	35.0	41.0	24.5	41.0
46-47	35.219887063776994	40.0	35.0	41.0	24.0	41.0
48-49	35.05823278498379	39.5	35.0	41.0	24.0	41.0
50-51	33.34317265396884	37.0	32.5	39.5	20.5	40.0
52-53	33.473219585931716	37.5	33.5	39.5	21.0	40.0
54-55	34.38534500230574	39.0	34.0	40.5	22.0	41.0
56-57	34.39556963278448	39.0	34.0	41.0	22.0	41.0
58-59	34.20177864111502	38.0	34.0	41.0	22.0	41.0
60-61	33.94514662593494	38.0	34.0	40.0	20.5	41.0
62-63	33.642046315659115	37.0	34.0	40.0	20.0	41.0
64-65	33.34688163620007	37.0	34.0	40.0	18.5	41.0
66-67	32.99531850340425	36.0	33.0	39.0	17.5	41.0
68-69	32.6231064949128	36.0	33.0	39.0	14.0	41.0
70-71	32.20134431256392	35.0	33.0	39.0	10.0	40.5
72-73	31.827532506243138	35.0	32.5	37.5	7.5	40.0
74-75	31.416901950464585	35.0	32.0	37.0	4.0	39.0
76-77	31.036775730591742	35.0	32.0	36.5	2.0	39.0
78-79	30.687180305927136	35.0	32.0	36.0	2.0	38.0
80-81	30.36327542405982	35.0	31.0	35.5	2.0	37.0
82-83	30.048639397865472	35.0	31.0	35.0	2.0	37.0
84-85	29.778617895632273	35.0	31.0	35.0	2.0	36.0
86-87	29.536098768436464	35.0	31.0	35.0	2.0	36.0
88-89	29.307492554496264	35.0	31.0	35.0	2.0	36.0
90-91	29.06804270639426	34.5	30.0	35.0	2.0	35.0
92-93	28.85896363771851	34.0	30.0	35.0	2.0	35.0
94-95	28.607136595879332	34.0	30.0	35.0	2.0	35.0
96-97	28.346297371601285	34.0	30.0	35.0	2.0	35.0
98-99	28.05196013033096	34.0	29.0	35.0	2.0	35.0
100-101	26.957055472017757	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83496.0
3	15218.0
4	10554.0
5	8279.0
6	7569.0
7	7742.0
8	8322.0
9	8717.0
10	9511.0
11	10244.0
12	10041.0
13	10283.0
14	11045.0
15	11713.0
16	11484.0
17	11989.0
18	11805.0
19	11164.0
20	11350.0
21	12206.0
22	13370.0
23	15739.0
24	18705.0
25	21986.0
26	25726.0
27	30400.0
28	35801.0
29	41749.0
30	50234.0
31	60850.0
32	75488.0
33	98281.0
34	137375.0
35	200832.0
36	334655.0
37	605526.0
38	625609.0
39	102784.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94424678227683	17.575320331572982	12.503430773298103	24.97700211285209
2	16.345855246140356	19.4864567504415	38.3763951964389	25.791292806979243
3	19.201129715553762	23.664983140724516	29.070650451021645	28.063236692700077
4	13.758925033322525	15.413199322742175	35.88623509492417	34.94164054901113
5	14.703102294823553	36.89923251325433	33.85118696403626	14.546478227885856
6	34.097667035458365	34.6791530199936	16.91799827113139	14.305181673416648
7	29.36567314643862	31.329875022682565	20.954403009363936	18.35004882151488
8	27.024281840593687	34.028422128631576	20.043977283324384	18.903318747450353
9	27.202340964942408	15.068737763972381	19.126616739713818	38.602304531371395
10-11	26.011928850305388	24.790906681913167	27.71074230198152	21.48642216579993
12-13	26.290429149855882	22.338188882107563	27.441051981557194	23.930329986479357
14-15	23.73994933273455	23.897255904263393	24.507251029848454	27.855543733153603
16-17	24.034969553687542	26.793925249041134	25.45234662831013	23.7187585689612
18-19	24.01592336851898	26.325852287967145	26.554218767382658	23.10400557613122
20-21	24.468649338809232	25.59850313006119	26.3918384365609	23.54100909456868
22-23	24.438404361823956	25.311255320834626	26.11944598497562	24.1308943323658
24-25	24.253485779682627	25.481892599615506	26.078459369737256	24.186162250964614
26-27	24.014490559298356	25.68714845027818	26.112100196722913	24.186260793700544
28-29	24.09068423432635	25.897470700565396	25.962672563516914	24.049172501591343
30-31	24.45865376306921	25.651552464068665	25.73342406175293	24.15636971110919
32-33	24.123587225091008	25.743168295230305	25.662109136625453	24.471135343053234
34-35	24.499474296131933	25.709790224266115	25.801106633192983	23.98962884640897
36-37	24.557784950432605	25.59866788296205	25.838189035053933	24.005358131551418
38-39	24.377044934053604	25.67107857316816	25.7794397152946	24.172436777483636
40-41	24.51488461179159	25.491874289122922	25.87899461393896	24.11424648514653
42-43	24.383962476886303	25.48966761376449	25.681919451585262	24.444450457763946
44-45	24.132807322076694	25.566780134809168	25.907094388962783	24.393318154151356
46-47	24.31305052944793	25.46556987773036	25.74021800510736	24.481161587714347
48-49	24.13871284319591	25.558076921481348	25.777532555351357	24.525677679971388
50-51	24.107567335543283	25.639538868308374	25.91667237790867	24.336221418239663
52-53	24.37535521157037	25.424788397164548	25.862306242290412	24.337550148974675
54-55	23.883232695393588	25.282682921682408	26.16112687558741	24.672957507336598
56-57	23.93997438811031	25.425770611258407	26.02415092978374	24.610104070847537
58-59	24.088242323666424	25.273723467100783	25.999972942868865	24.638061266363927
60-61	24.061725854042017	25.292205853623518	26.200350710160116	24.445717582174357
62-63	24.046460606349186	25.322054918155196	26.168928049150892	24.46255642634473
64-65	23.809808954395827	25.158723702889933	26.161071484419455	24.870395858294785
66-67	23.9304053543444	25.18541323872438	25.98653045257225	24.897650954358973
68-69	24.01568107477078	25.18575602644148	26.015799023693127	24.782763875094616
70-71	23.846997822972615	24.97334620217645	26.10297671345328	25.076679261397654
72-73	23.85447796861445	25.225363823114062	25.937517471986204	24.98264073628528
74-75	23.865591564628403	25.309451341581042	26.034762866993276	24.790194226797283
76-77	23.885524172307075	24.937758804795923	26.094160085165612	25.082556937731393
78-79	23.848206513485014	24.97922982045835	26.10853956507262	25.064024100984017
80-81	24.02347590443819	25.11107831927995	25.933553968639384	24.93189180764248
82-83	23.962360779965916	25.109692064884232	26.00019879234552	24.927748362804326
84-85	23.883560923545527	25.059124511685017	26.052575043620728	25.00473952114873
86-87	23.81754461929827	25.16410249471154	25.967768561492765	25.05058432449743
88-89	23.772422563196663	24.891771494568143	26.105994570218684	25.22981137201651
90-91	23.862268691625456	24.86976260756594	26.146111605349176	25.121857095459433
92-93	23.94476551086364	24.685683853432067	26.205460378214916	25.164090257489374
94-95	23.85950080428142	24.579890150096446	26.236551108493916	25.324057937128213
96-97	23.917039890691356	24.458278626987852	26.26563134024517	25.359050142075617
98-99	23.712846377600183	24.421058480765513	26.1945565361816	25.6715386054527
100-101	24.018490709629063	24.260441078589846	26.321223124970345	25.399845086810746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	39.5
2	71.5
3	119.0
4	111.0
5	121.0
6	186.0
7	350.5
8	525.5
9	558.0
10	514.5
11	471.5
12	494.5
13	584.5
14	678.0
15	745.5
16	812.5
17	915.5
18	1012.5
19	1079.5
20	1179.0
21	1275.0
22	1351.0
23	1443.0
24	1549.5
25	1729.5
26	2040.0
27	2486.0
28	3128.0
29	4282.0
30	6332.5
31	9653.0
32	14981.0
33	20715.5
34	26499.5
35	33603.0
36	42684.0
37	51733.5
38	60146.0
39	68008.0
40	74162.0
41	80477.0
42	87326.0
43	94521.5
44	101722.5
45	109396.0
46	114788.0
47	118754.5
48	122818.0
49	124818.0
50	123999.5
51	123201.0
52	122715.0
53	121317.5
54	119179.0
55	113018.0
56	104400.5
57	94408.5
58	85285.5
59	75229.0
60	62394.5
61	51825.0
62	43426.5
63	34077.5
64	25156.5
65	19016.5
66	14628.5
67	11619.5
68	9278.0
69	7302.0
70	5817.5
71	4377.0
72	3249.0
73	2499.0
74	1978.5
75	1555.0
76	1155.5
77	747.5
78	474.5
79	332.0
80	225.5
81	177.0
82	140.5
83	101.5
84	80.0
85	72.0
86	62.5
87	42.5
88	37.0
89	40.0
90	35.5
91	31.5
92	26.0
93	18.0
94	18.0
95	16.0
96	11.5
97	12.0
98	10.5
99	8.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054322568057106016
2	0.07477002906203392
3	0.0695141676065419
4	0.07023414862784218
5	0.06397031374252975
6	0.011231703932284344
7	0.01393163276216039
8	0.018287517941027075
9	0.006947816855547688
10-11	0.013787636557900334
12-13	0.022175415456048578
14-15	0.039868949054502924
16-17	0.026981288773227936
18-19	0.01072771721737415
20-21	0.021203441077293203
22-23	0.03711502164802936
24-25	0.03461308759901089
26-27	0.02140143585815078
28-29	0.011627693493999497
30-31	0.01191568590251961
32-33	0.008999762766253482
34-35	0.00527386098102454
36-37	6.119838681052368E-4
38-39	0.0036899027341639277
40-41	0.0011699691596129527
42-43	0.2241300919307767
44-45	0.1948808629404529
46-47	0.20074870826405017
48-49	0.20751652986427277
50-51	0.19947074195124218
52-53	0.20647255738338735
54-55	0.20229666745984576
56-57	0.20523059012164438
58-59	0.21378036474958517
60-61	0.21766826226460673
62-63	0.2035026356705237
64-65	0.20373662950244634
66-67	0.20150468833641544
68-69	0.1967168145447686
70-71	0.18946300575516828
72-73	0.20001072771721737
74-75	0.19889475713420193
76-77	0.19333290374465728
78-79	0.21052245062820144
80-81	0.22168215645835576
82-83	0.22011619773702765
84-85	0.21406835715810532
86-87	0.23357984283534286
88-89	0.22623603641808002
90-91	0.2328958608651076
92-93	0.22888196667135854
94-95	0.2315818955012346
96-97	0.23815172232059964
98-99	0.2316718931288971
100-101	0.2383677166269897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2777851.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.37153650825121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.45292294125282	43.0976873674535
2	12.450738193042564	14.037344852075531
3	4.389883076833386	7.423933623980021
4	2.1002745897832407	4.735828228612731
5	1.1238084522695495	3.1675404597697097
6	0.7637334295224059	2.583169614293652
7	0.5129968694574888	2.0242895228668987
8	0.36102782179011356	1.628135442922864
9	0.27221766471116676	1.381079522201078
>10	1.4824339971419944	14.782848483978894
>50	0.06853402615739167	2.566424266028308
>100	0.019892348473818707	1.8153463022690657
>500	0.0011524421731157388	0.4465617519655789
>1k	3.8414739098338265E-4	0.3098105615822821
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5999051065013924E-5	0.0	0.0	0.0	7.199810213002785E-5
2	3.5999051065013924E-5	0.0	0.0	0.0	7.199810213002785E-5
3	3.5999051065013924E-5	0.0	0.0	0.0	7.199810213002785E-5
4	3.5999051065013924E-5	0.0	0.0	0.0	7.199810213002785E-5
5	3.5999051065013924E-5	0.0	0.0	0.0	7.199810213002785E-5
6	7.199810213002785E-5	0.0	0.0	0.0	1.0799715319504178E-4
7	7.199810213002785E-5	0.0	0.0	3.5999051065013924E-5	1.0799715319504178E-4
8	7.199810213002785E-5	0.0	0.0	3.5999051065013924E-5	1.0799715319504178E-4
9	7.199810213002785E-5	0.0	0.0	7.199810213002785E-5	1.0799715319504178E-4
10-11	1.0799715319504178E-4	0.0	0.0	7.199810213002785E-5	1.0799715319504178E-4
12-13	1.0799715319504178E-4	0.0	0.0	1.439962042600557E-4	1.439962042600557E-4
14-15	1.2599667872754874E-4	0.0	0.0	1.439962042600557E-4	2.519933574550975E-4
16-17	1.7999525532506964E-4	0.0	0.0	1.439962042600557E-4	3.239914595851253E-4
18-19	2.1599430639008356E-4	0.0	0.0	1.7999525532506964E-4	3.239914595851253E-4
20-21	2.519933574550975E-4	0.0	0.0	1.979947808575766E-4	3.959895617151532E-4
22-23	2.519933574550975E-4	0.0	0.0	2.879924085201114E-4	4.319886127801671E-4
24-25	3.599905106501393E-4	0.0	0.0	4.4998813831267405E-4	4.85987189377688E-4
26-27	4.319886127801671E-4	0.0	0.0	9.89973904287883E-4	5.03986714910195E-4
28-29	4.319886127801671E-4	0.0	0.0	0.0034739084277738436	5.03986714910195E-4
30-31	5.03986714910195E-4	0.0	0.0	0.01429162327281053	5.03986714910195E-4
32-33	5.399857659752089E-4	0.0	0.0	0.0436848484673944	5.03986714910195E-4
34-35	5.399857659752089E-4	0.0	0.0	0.08808967795608907	5.03986714910195E-4
36-37	5.399857659752089E-4	0.0	0.0	0.16289570606918802	5.03986714910195E-4
38-39	5.399857659752089E-4	0.0	0.0	0.28712843129455107	5.399857659752089E-4
40-41	5.399857659752089E-4	0.0	0.0	0.4232588428969012	5.399857659752089E-4
42-43	5.399857659752089E-4	0.0	0.0	0.5692889935421303	5.399857659752089E-4
44-45	5.579852915077159E-4	0.0	0.0	0.7506342132821379	5.759848170402229E-4
46-47	5.759848170402228E-4	0.0	0.0	0.9668085149275465	6.659824447027575E-4
48-49	5.759848170402228E-4	0.0	0.0	1.1928285570392365	7.559800723652924E-4
50-51	5.759848170402228E-4	0.0	0.0	1.4284063472086874	7.559800723652924E-4
52-53	5.759848170402228E-4	0.0	0.0	1.6679800320463554	8.819767510928412E-4
54-55	5.759848170402228E-4	0.0	0.0	1.9437867617809594	9.359753276903622E-4
56-57	5.759848170402228E-4	0.0	0.0	2.2623423646552676	9.71974378755376E-4
58-59	5.759848170402228E-4	0.0	0.0	2.5941996168980985	9.71974378755376E-4
60-61	6.299833936377437E-4	0.0	0.0	2.944722377118139	9.71974378755376E-4
62-63	6.479829191702506E-4	0.0	0.0	3.3099687492237706	0.0010439724808854038
64-65	6.479829191702506E-4	0.0	0.0	3.7037083702473605	0.0010799715319504176
66-67	6.479829191702506E-4	0.0	0.0	4.1576744037027185	0.0011159705830154317
68-69	6.839819702352646E-4	0.0	0.0	4.6387297230844995	0.0011339701085479386
70-71	6.839819702352646E-4	0.0	0.0	5.146262344524598	0.0011699691596129525
72-73	6.839819702352646E-4	0.0	0.0	5.668842569309874	0.0013679639404705292
74-75	6.839819702352646E-4	0.0	0.0	6.210448292583008	0.001403962991535543
76-77	6.839819702352646E-4	0.0	0.0	6.815412345730566	0.001403962991535543
78-79	6.839819702352646E-4	0.0	0.0	7.48796461725269	0.001403962991535543
80-81	6.839819702352646E-4	0.0	0.0	8.178444416205188	0.0014399620426005571
82-83	6.839819702352646E-4	0.0	0.0	8.89873142943952	0.001547959195795599
84-85	6.839819702352646E-4	0.0	0.0	9.640995863349042	0.001547959195795599
86-87	6.839819702352646E-4	0.0	0.0	10.42465920598333	0.0015659587213281059
88-89	7.199810213002786E-4	0.0	0.0	11.273120840534645	0.0015839582468606128
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2185	0.0	52.353527	1
GTATCAA	3890	0.0	42.096874	1
TCAACGC	4570	0.0	33.340878	4
CAACGCA	4815	0.0	31.740696	5
ATCAACG	4950	0.0	30.877264	3
TATCAAC	5075	0.0	30.211363	2
AACGCAG	5135	0.0	29.743944	6
GTACATG	8045	0.0	28.43815	1
TACATGG	8415	0.0	26.737947	2
ACATGGG	8850	0.0	24.832811	3
ACGCAGA	6135	0.0	24.2833	7
CATGGGG	7435	0.0	23.430052	4
CGCAGAG	6400	0.0	22.908804	8
ATGGGGG	4695	0.0	20.420795	5
GAGTACA	6130	0.0	20.364588	1
TAAGGCG	685	0.0	20.093903	5
AGTACAT	6115	0.0	18.863125	2
GCAGAGT	7500	0.0	18.157999	9
GGTATAT	435	3.6379788E-12	17.458666	1
TATACTG	1065	0.0	17.380886	5
>>END_MODULE