FastQCFastQC Report
Mon 15 Aug 2016
SRR2921665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2777851
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.047.3102871
GTATCAA38750.043.552731
TCAACGC48350.034.6535874
CAACGCA50050.033.4081765
TATCAAC51650.032.4395142
ATCAACG52700.031.7931863
AACGCAG54650.030.6753236
GTACATG80450.027.0052911
TACATGG84650.025.1761532
ACGCAGA66450.024.9413197
CGCAGAG69800.023.1319628
ACATGGG91350.022.965913
TAAGGCG7050.022.2350965
CATGGGG70800.021.6540534
GCAGAGT77950.019.6168259
ATGGGGG41100.019.1858235
GTATTAA14150.018.8143981
TATACTG9650.017.7210465
AGAGTAC66700.017.65691810-11
TATTAAG11950.017.476512
Produced by FastQC (version 0.11.5)