##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2777851
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22726704924058	33.0	31.0	34.0	28.0	34.0
2	31.376383398533616	34.0	31.0	34.0	28.0	34.0
3	31.443416511540757	34.0	31.0	34.0	28.0	34.0
4	34.92477242299893	37.0	35.0	37.0	32.0	37.0
5	34.65353109292039	37.0	35.0	37.0	32.0	37.0
6	34.61931147494952	37.0	35.0	37.0	32.0	37.0
7	34.491039656194665	37.0	35.0	37.0	31.0	37.0
8	34.43299442626692	37.0	35.0	37.0	31.0	37.0
9	36.00558705272529	39.0	37.0	39.0	31.0	39.0
10-11	35.894536100028404	39.0	37.0	39.0	30.0	39.0
12-13	35.8066418969196	39.0	37.0	39.0	30.0	39.0
14-15	37.017031871039876	40.0	37.0	41.0	31.0	41.0
16-17	36.979572878458924	40.0	37.0	41.0	31.0	41.0
18-19	36.933072184217224	40.0	37.0	41.0	30.5	41.0
20-21	36.86965553588007	40.0	37.0	41.0	30.0	41.0
22-23	36.774287389784405	40.0	37.0	41.0	30.0	41.0
24-25	36.67901392119303	40.0	37.0	41.0	30.0	41.0
26-27	36.54584191160721	40.0	37.0	41.0	30.0	41.0
28-29	36.41762949128661	40.0	37.0	41.0	29.5	41.0
30-31	36.257065983740674	40.0	36.0	41.0	29.0	41.0
32-33	36.20267177757194	40.0	36.0	41.0	28.5	41.0
34-35	36.326930062123566	40.0	37.0	41.0	29.5	41.0
36-37	36.31213697206942	40.0	37.0	41.0	29.0	41.0
38-39	36.20227560801497	40.0	36.0	41.0	29.0	41.0
40-41	36.11110315132093	40.0	36.0	41.0	28.0	41.0
42-43	35.98361215198368	40.0	36.0	41.0	28.0	41.0
44-45	35.8457860050809	40.0	35.5	41.0	27.0	41.0
46-47	35.68046396296994	40.0	35.0	41.0	27.0	41.0
48-49	35.53072896998435	40.0	35.0	41.0	26.0	41.0
50-51	35.3696090971042	39.0	35.0	41.0	26.0	41.0
52-53	35.18153313478656	39.0	35.0	41.0	26.0	41.0
54-55	34.96125188140041	39.0	35.0	41.0	25.0	41.0
56-57	34.72329005407418	39.0	35.0	41.0	24.5	41.0
58-59	34.47390644782604	38.0	34.0	40.0	24.0	41.0
60-61	34.18740026012914	38.0	34.0	40.0	23.0	41.0
62-63	33.878232669786826	37.0	34.0	40.0	22.5	41.0
64-65	33.536696712674654	36.5	34.0	40.0	22.0	41.0
66-67	33.18384697379378	36.0	33.5	39.0	21.5	41.0
68-69	32.816594014581774	35.5	33.0	39.0	20.5	40.5
70-71	32.42003350791673	35.0	33.0	38.5	19.5	40.0
72-73	32.06168149407581	35.0	33.0	37.0	18.0	39.5
74-75	31.658502021886704	35.0	32.0	37.0	16.5	39.0
76-77	28.976727873453257	32.0	28.0	34.5	9.0	36.5
78-79	30.54238672268599	34.5	31.0	35.5	10.0	37.0
80-81	30.72733256751352	35.0	32.0	35.0	9.0	37.0
82-83	30.560733639061276	35.0	32.0	35.0	7.0	37.0
84-85	30.366372782413457	35.0	32.0	35.0	4.5	36.0
86-87	30.15700986122006	35.0	31.0	35.0	2.0	36.0
88-89	29.892744607252155	34.5	31.0	35.0	2.0	35.5
90-91	29.698501467501316	34.0	31.0	35.0	2.0	35.0
92-93	29.533145946272857	34.0	31.0	35.0	2.0	35.0
94-95	29.42124991585222	34.0	31.0	35.0	2.0	35.0
96-97	29.205646379161443	34.0	31.0	35.0	2.0	35.0
98-99	28.99418111338585	34.0	31.0	35.0	2.0	35.0
100-101	27.869754173279993	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	58490.0
3	20062.0
4	11207.0
5	7843.0
6	6787.0
7	6901.0
8	7120.0
9	7545.0
10	8255.0
11	8847.0
12	9463.0
13	9834.0
14	10685.0
15	11091.0
16	12210.0
17	12677.0
18	12006.0
19	11271.0
20	10663.0
21	10957.0
22	11206.0
23	12673.0
24	14994.0
25	17928.0
26	21333.0
27	26144.0
28	31111.0
29	37616.0
30	45744.0
31	56626.0
32	72148.0
33	94822.0
34	135295.0
35	202392.0
36	351778.0
37	662435.0
38	656716.0
39	72973.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.903742897084484	19.58953360451125	13.290506405108943	26.216217093295324
2	15.443219154978657	21.09644627655879	36.89386060499372	26.56647396346883
3	17.886663875248825	24.79034663865168	28.94283869913208	28.380150786967416
4	13.264141596692506	16.664014584770378	34.913554130551546	35.158289687985565
5	14.405039680825016	37.25719800083539	33.98244969681257	14.355312621527027
6	34.06698240768607	35.29514681381408	16.579031493942697	14.058839284557154
7	29.10480079745098	31.558388120889134	21.020241906423344	18.31656917523654
8	26.973440980095763	34.57698055079268	19.765134991041634	18.684443478069916
9	26.99838832248382	15.10775775950546	18.967000029879213	38.926853888131504
10-11	25.94581103012203	25.069385767579828	27.70807986728644	21.276723335011702
12-13	26.27601033609591	22.54676118621748	27.475486331456334	23.70174214623027
14-15	23.585923705734825	24.10646125012173	24.53471039316443	27.77290465097902
16-17	23.977725947144034	27.068946462571247	25.37884501364544	23.57448257663928
18-19	23.97502568116974	26.561142423708432	26.548326588520073	22.91550530660176
20-21	24.43373309209091	25.721963308208313	26.37203627852124	23.472267321179537
22-23	24.33849043739207	25.476258445827366	26.151582644281497	24.033668472499066
24-25	24.19855808674159	25.70059006341985	26.03885685157192	24.061994998266638
26-27	23.95770502054431	25.877963903863392	26.096484004382642	24.067847071209652
28-29	24.03499661140207	26.079261690662857	25.949909952397626	23.935831745537445
30-31	24.35382004715988	25.805665882108954	25.79958096912861	24.040933101602565
32-33	24.130509333474947	25.879004609356787	25.653748286455187	24.336737770713075
34-35	24.44822434691224	25.875612566517574	25.784363264748443	23.891799821821742
36-37	24.476231774021358	25.743902893422206	25.856566770402395	23.923298562154045
38-39	24.419524301339415	25.81358755383208	25.67686315788716	24.090024986941344
40-41	24.429372307288	25.67554164742899	25.84555319453407	24.049532850748943
42-43	24.32157987149305	25.641535674953097	25.657196716725593	24.379687736828252
44-45	24.12813057291075	25.643377273693584	25.829288721306177	24.39920343208949
46-47	24.319467194671944	25.630168301683014	25.673854738547387	24.37650976509765
48-49	24.05563859073529	25.712047917549096	25.77454781629278	24.45776567542283
50-51	24.089843081327817	25.74083984078633	25.830407881987625	24.338909195898232
52-53	24.358226901397423	25.562636518026455	25.742779563268314	24.336357017307808
54-55	23.842188377233995	25.444810230801814	26.02272827501742	24.690273116946774
56-57	23.884475031825854	25.545611776429862	25.85724592600273	24.712667265741555
58-59	23.99931446886549	25.35930526604207	25.90902634596243	24.73235391913001
60-61	23.93232796857179	25.504098455086922	26.04464882317024	24.518924753171046
62-63	23.94696723105906	25.473235529643564	25.99032717091353	24.589470068383847
64-65	23.65330764852362	25.29071426783617	25.976212740234633	25.07976534340558
66-67	23.797956221206455	25.421993134574585	25.737078700251782	25.042971943967178
68-69	23.820285016337973	25.420568826124185	25.715417660496144	25.043728497041695
70-71	23.677394173792713	25.255081410455343	25.78454340567117	25.28298101008078
72-73	23.664988736676627	25.54843319078401	25.61539554332144	25.17118252921793
74-75	23.61320837095498	25.573182840748586	25.625287507393047	25.188321280903388
76-77	23.62954181141739	25.29358134789277	25.729664515053653	25.347212325636185
78-79	23.542284871814456	25.398791860805304	25.770831862131182	25.28809140524906
80-81	23.705051876814068	25.51440492282643	25.463451183742354	25.31709201661715
82-83	23.587406241785466	25.550756367276538	25.522777439694067	25.339059951243932
84-85	23.50430575032285	25.513625886762703	25.558971374175364	25.423096988739086
86-87	23.39597004043216	25.6738993547569	25.427070094898863	25.503060509912075
88-89	23.273697961653355	25.471688406491676	25.54611303264918	25.708500599205784
90-91	23.25779926134541	25.42747752472186	25.579223223049024	25.735499990883703
92-93	23.300962656061973	25.302038877229027	25.590814084954477	25.80618438175452
94-95	23.17734590046276	25.241943163495634	25.632735309201752	25.947975626839852
96-97	23.064032011054884	25.226222003873744	25.632462985822613	26.077282999248762
98-99	22.816611689640858	25.20997527923805	25.560267072067482	26.413145959053608
100-101	22.859380458927447	25.222613992529823	25.56201359578364	26.35599195275909
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	42.5
2	65.5
3	98.0
4	89.5
5	93.0
6	165.5
7	307.5
8	455.0
9	486.5
10	395.0
11	325.0
12	325.0
13	363.5
14	400.0
15	415.0
16	463.0
17	517.0
18	582.0
19	659.0
20	720.5
21	822.0
22	938.5
23	1122.0
24	1306.0
25	1517.5
26	1969.0
27	2547.5
28	3371.0
29	4760.5
30	6947.5
31	10451.5
32	15977.5
33	21650.0
34	27575.0
35	34531.0
36	43116.0
37	52005.0
38	60338.0
39	68361.0
40	74712.0
41	80992.0
42	87892.5
43	95181.0
44	102274.5
45	110144.5
46	115658.5
47	119577.5
48	123886.5
49	125682.5
50	125228.0
51	124315.5
52	123195.0
53	122218.0
54	119749.5
55	112973.5
56	103962.0
57	94121.0
58	84761.5
59	74090.5
60	61572.0
61	51010.5
62	42506.5
63	33012.5
64	24013.0
65	18169.5
66	13994.5
67	11153.0
68	8946.5
69	6810.5
70	5185.0
71	3920.5
72	2935.0
73	2261.0
74	1753.0
75	1317.0
76	942.5
77	578.5
78	336.0
79	197.0
80	108.0
81	63.0
82	45.5
83	28.0
84	20.5
85	14.0
86	12.0
87	8.5
88	7.5
89	6.0
90	3.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16264371271173292
2	1.439962042600557E-4
3	0.010439724808854038
4	0.005723849119337214
5	0.024875344285924625
6	0.0016919554000556546
7	0.0
8	0.0
9	0.0
10-11	0.04841872368244373
12-13	4.679876638451811E-4
14-15	0.005237861929959526
16-17	0.0
18-19	0.0013499644149380222
20-21	3.2399145958512536E-4
22-23	0.0
24-25	5.03986714910195E-4
26-27	0.0026819293043435373
28-29	0.005525854338479638
30-31	0.017639535021856823
32-33	0.012545669296157354
34-35	0.010637719589711615
36-37	0.004301886602269165
38-39	0.0
40-41	0.003545906529903872
42-43	0.009287755174773593
44-45	0.006641824921495069
46-47	0.0036359041575664062
48-49	0.008873766087525933
50-51	0.0026819293043435373
52-53	0.0021059444873033147
54-55	0.004373884704399192
56-57	0.008459777000278272
58-59	0.015929580096268665
60-61	0.007883792183238049
62-63	0.021131442975163176
64-65	0.04004894430982799
66-67	0.058354461776387576
68-69	0.04836472510584621
70-71	0.06571626771918292
72-73	0.03302912935215028
74-75	0.026945289722162924
76-77	0.22074618113066538
78-79	0.08434577664532764
80-81	0.06492428859575261
82-83	0.027395277860475596
84-85	0.05002068145483685
86-87	0.06634625111282066
88-89	0.285634470675353
90-91	0.29107032738617006
92-93	0.05291860506557047
94-95	0.010349727181191504
96-97	0.015677586738813566
98-99	8.639772255603342E-4
100-101	1.6199572979256268E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2777851.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.19517755912001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16411866710061	42.80056172131606
2	12.672275331141877	14.242415246231483
3	4.401880809749474	7.42093421093864
4	2.1075045838119753	4.73726377175893
5	1.1470979076715078	3.2230685299647717
6	0.7390487565333819	2.491858565894415
7	0.5126284821216941	2.0165074002283543
8	0.35986822779442346	1.6178287167034777
9	0.28879681825060677	1.4606089632091586
>10	1.519375793916803	14.987556962597576
>50	0.06668932866159968	2.513839751012824
>100	0.01936695294822613	1.8143557742660748
>500	9.631002127498881E-4	0.3728616565501952
>1k	3.852400850594457E-4	0.3003387293281292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.5999051065013924E-5	0.0	0.0	0.0	3.5999051065013924E-5
6	3.5999051065013924E-5	0.0	0.0	0.0	3.5999051065013924E-5
7	3.5999051065013924E-5	0.0	0.0	0.0	3.5999051065013924E-5
8	3.5999051065013924E-5	0.0	0.0	0.0	3.5999051065013924E-5
9	3.5999051065013924E-5	0.0	0.0	0.0	1.0799715319504178E-4
10-11	1.0799715319504178E-4	0.0	0.0	0.0	1.0799715319504178E-4
12-13	1.0799715319504178E-4	0.0	0.0	0.0	3.599905106501393E-4
14-15	1.2599667872754874E-4	0.0	0.0	0.0	3.779900361826462E-4
16-17	1.6199572979256265E-4	0.0	0.0	0.0	3.959895617151532E-4
18-19	1.7999525532506964E-4	0.0	0.0	3.5999051065013924E-5	4.6798766384518103E-4
20-21	1.7999525532506964E-4	0.0	0.0	3.5999051065013924E-5	4.85987189377688E-4
22-23	1.7999525532506964E-4	0.0	0.0	8.999762766253482E-5	6.659824447027575E-4
24-25	1.7999525532506964E-4	0.0	0.0	2.3399383192259052E-4	7.559800723652924E-4
26-27	1.979947808575766E-4	0.0	0.0	7.919791234303064E-4	7.559800723652924E-4
28-29	2.1599430639008356E-4	0.0	0.0	0.00325791412138376	7.739795978977994E-4
30-31	2.1599430639008356E-4	0.0	0.0	0.014021630389822924	8.099786489628133E-4
32-33	2.1599430639008356E-4	0.0	0.0	0.043540852263134346	8.279781744953203E-4
34-35	2.3399383192259052E-4	0.0	0.0	0.08799968032842653	8.819767510928412E-4
36-37	2.519933574550975E-4	0.0	0.0	0.16300370322238306	8.999762766253482E-4
38-39	2.519933574550975E-4	0.0	0.0	0.28675044125836846	9.179758021578551E-4
40-41	2.519933574550975E-4	0.0	0.0	0.42205287468622327	9.359753276903621E-4
42-43	2.519933574550975E-4	0.0	0.0	0.5676690362442046	9.359753276903621E-4
44-45	2.519933574550975E-4	0.0	0.0	0.7478442868245994	9.539748532228691E-4
46-47	2.519933574550975E-4	0.0	0.0	0.9616066520486519	0.0010259729553528968
48-49	2.519933574550975E-4	0.0	0.0	1.1848187681772708	0.0010799715319504178
50-51	2.519933574550975E-4	0.0	0.0	1.419010594880719	0.0010799715319504178
52-53	2.699928829876044E-4	0.0	0.0	1.6568383257417334	0.0010799715319504178
54-55	2.879924085201114E-4	0.0	0.0	1.929855129018799	0.0010799715319504178
56-57	2.879924085201114E-4	0.0	0.0	2.2462507888292063	0.0010799715319504178
58-59	2.879924085201114E-4	0.0	0.0	2.576704078080502	0.0010799715319504178
60-61	3.059919340526184E-4	0.0	0.0	2.923932925128094	0.0010799715319504178
62-63	3.239914595851253E-4	0.0	0.0	3.285489394499561	0.0010799715319504178
64-65	3.239914595851253E-4	0.0	0.0	3.676151096657092	0.0011159705830154317
66-67	3.239914595851253E-4	0.0	0.0	4.1253832548974	0.0011159705830154317
68-69	3.779900361826462E-4	0.0	0.0	4.603432653515253	0.0011159705830154317
70-71	4.319886127801671E-4	0.0	0.0	5.106735386455213	0.0011699691596129525
72-73	4.6798766384518103E-4	0.0	0.0	5.6278396501468215	0.001331964889405515
74-75	4.6798766384518103E-4	3.5999051065013924E-5	0.0	6.169445373419957	0.0013679639404705292
76-77	5.219862404427019E-4	3.5999051065013924E-5	0.0	6.7696215527758685	0.0013679639404705292
78-79	5.399857659752089E-4	5.399857659752089E-5	0.0	7.439707889300038	0.0013859634660030361
80-81	5.399857659752089E-4	7.199810213002785E-5	0.0	8.127793751356714	0.001493960619198078
82-83	5.399857659752089E-4	7.199810213002785E-5	0.0	8.846586803971848	0.0016559563489906405
84-85	5.399857659752089E-4	7.199810213002785E-5	0.0	9.588239254013263	0.0016559563489906405
86-87	5.399857659752089E-4	7.199810213002785E-5	0.0	10.370804625590068	0.0016919554000556546
88-89	5.399857659752089E-4	7.199810213002785E-5	0.0	11.219230261090317	0.0017459539766531754
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2070	0.0	47.310287	1
GTATCAA	3875	0.0	43.55273	1
TCAACGC	4835	0.0	34.653587	4
CAACGCA	5005	0.0	33.408176	5
TATCAAC	5165	0.0	32.439514	2
ATCAACG	5270	0.0	31.793186	3
AACGCAG	5465	0.0	30.675323	6
GTACATG	8045	0.0	27.005291	1
TACATGG	8465	0.0	25.176153	2
ACGCAGA	6645	0.0	24.941319	7
CGCAGAG	6980	0.0	23.131962	8
ACATGGG	9135	0.0	22.96591	3
TAAGGCG	705	0.0	22.235096	5
CATGGGG	7080	0.0	21.654053	4
GCAGAGT	7795	0.0	19.616825	9
ATGGGGG	4110	0.0	19.185823	5
GTATTAA	1415	0.0	18.814398	1
TATACTG	965	0.0	17.721046	5
AGAGTAC	6670	0.0	17.656918	10-11
TATTAAG	1195	0.0	17.47651	2
>>END_MODULE