FastQCFastQC Report
Mon 15 Aug 2016
SRR2921664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639368
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.047.5903661
GTATCAA32500.040.4554521
TCAACGC37100.034.1600074
CAACGCA38250.033.1323475
GTACATG70300.031.1262111
AACGCAG41900.030.4559356
ATCAACG41950.030.210643
GAGTACA51250.029.8224031
TATCAAC43000.029.6942732
TACATGG73700.029.2399922
ACATGGG78100.027.5313683
CATGGGG58900.025.787864
ACGCAGA49250.025.239357
AGTACAT55700.024.7133542
CCCTATA4200.023.7333412
CGCAGAG52100.023.4953298
ATGGGGG38100.020.8048175
ACCGCGT1404.891408E-420.3339988
GCAGAGT61750.018.3637129
AGAGTAC49750.017.40481210-11
Produced by FastQC (version 0.11.5)