##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921664_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2639368
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.644477390041857	31.0	31.0	34.0	27.0	34.0
2	30.6783885384683	33.0	31.0	34.0	27.0	34.0
3	30.74201096626162	33.0	31.0	34.0	27.0	34.0
4	34.21528449234817	37.0	35.0	37.0	30.0	37.0
5	34.21945859766429	37.0	35.0	37.0	30.0	37.0
6	34.27319153676183	37.0	35.0	37.0	30.0	37.0
7	34.186931492690675	37.0	35.0	37.0	30.0	37.0
8	34.16199143128203	37.0	35.0	37.0	30.0	37.0
9	35.74270696621312	39.0	37.0	39.0	30.0	39.0
10-11	35.64863046759679	39.0	37.0	39.0	30.0	39.0
12-13	35.54287427899406	39.0	37.0	39.0	30.0	39.0
14-15	36.706944806483975	40.0	37.0	41.0	30.0	41.0
16-17	36.64401326378133	40.0	37.0	41.0	30.0	41.0
18-19	36.606808334419455	40.0	37.0	41.0	30.0	41.0
20-21	36.53210143488896	40.0	37.0	41.0	29.0	41.0
22-23	36.435173496079365	40.0	37.0	41.0	29.0	41.0
24-25	36.32649179652098	40.0	37.0	41.0	29.0	41.0
26-27	36.18263444127534	40.0	36.0	41.0	28.0	41.0
28-29	36.04366405139412	40.0	36.0	41.0	27.0	41.0
30-31	35.892393557851726	40.0	36.0	41.0	27.0	41.0
32-33	35.71173099014612	40.0	36.0	41.0	27.0	41.0
34-35	35.533670939406704	39.0	35.0	41.0	26.0	41.0
36-37	35.501952740201446	39.0	35.0	41.0	25.5	41.0
38-39	35.68130571409519	40.0	35.5	41.0	26.5	41.0
40-41	35.73894792238142	40.0	36.0	41.0	26.5	41.0
42-43	35.569702102927664	40.0	35.0	41.0	26.0	41.0
44-45	35.44593270055559	40.0	35.0	41.0	25.0	41.0
46-47	35.28002555914901	40.0	35.0	41.0	24.0	41.0
48-49	35.10791788034105	39.5	35.0	41.0	24.0	41.0
50-51	33.37744016749464	37.0	32.5	39.5	21.0	40.0
52-53	33.49244516111433	37.5	33.5	39.5	21.5	40.0
54-55	34.41484552362535	39.0	34.0	40.5	22.5	41.0
56-57	34.43077888342967	39.0	34.0	41.0	22.0	41.0
58-59	34.235970126181726	38.0	34.0	41.0	22.0	41.0
60-61	33.97796461122511	38.0	34.0	40.0	21.5	41.0
62-63	33.67274552089743	37.0	34.0	40.0	20.0	41.0
64-65	33.37483670333201	37.0	34.0	40.0	19.5	41.0
66-67	33.02735976945996	36.0	33.0	39.0	18.0	41.0
68-69	32.655701289096484	36.0	33.0	39.0	15.5	41.0
70-71	32.24378923287696	35.0	33.0	39.0	12.0	40.5
72-73	31.869081537701447	35.0	32.5	37.5	7.5	40.0
74-75	31.464481838076388	35.0	32.0	37.0	4.5	39.0
76-77	31.074554590341325	35.0	32.0	36.5	2.0	39.0
78-79	30.710143488895827	35.0	32.0	36.0	2.0	38.5
80-81	30.376829225784356	35.0	31.0	36.0	2.0	37.0
82-83	30.042079012854593	35.0	31.0	35.0	2.0	37.0
84-85	29.746862506478827	35.0	31.0	35.0	2.0	36.0
86-87	29.477231291733474	35.0	31.0	35.0	2.0	36.0
88-89	29.209637496552205	35.0	31.0	35.0	2.0	36.0
90-91	28.941518575659025	35.0	30.0	35.0	2.0	35.0
92-93	28.704345320546434	34.0	30.0	35.0	2.0	35.0
94-95	28.423129703777573	34.0	30.0	35.0	2.0	35.0
96-97	28.127983668817688	34.0	29.0	35.0	2.0	35.0
98-99	27.80309566532594	34.0	29.0	35.0	2.0	35.0
100-101	26.67629068777071	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74609.0
3	13729.0
4	9698.0
5	7649.0
6	6896.0
7	7133.0
8	7812.0
9	8346.0
10	9016.0
11	9678.0
12	9785.0
13	9921.0
14	10615.0
15	11639.0
16	11732.0
17	12677.0
18	11936.0
19	11219.0
20	11664.0
21	12202.0
22	13425.0
23	15613.0
24	17739.0
25	21038.0
26	24734.0
27	29613.0
28	34895.0
29	41399.0
30	49009.0
31	59784.0
32	72849.0
33	94813.0
34	130710.0
35	187985.0
36	313375.0
37	566152.0
38	593078.0
39	105190.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.1798412552202	17.09320939428848	10.793682201881602	25.933267148609723
2	17.362756687855587	19.74724952247206	36.59604283078715	26.293950958885205
3	20.156520605033045	22.611244733864925	28.970681010976612	28.261553650125414
4	13.664648315097905	14.441785071528201	35.608809571922684	36.28475704145121
5	14.29886962640349	36.94485978757348	34.23815791868104	14.518112667342
6	34.766461874473784	34.09089014504198	16.492263644624032	14.650384335860206
7	29.928583310692346	31.252067529638854	20.301369561947073	18.51797959772173
8	28.000854894212523	33.220181111764155	19.798266186622346	18.980697807400972
9	27.445860775215653	14.380834596967127	18.677606287326455	39.49569834049076
10-11	26.380037645873024	24.54412401350098	27.54730600865647	21.52853233196953
12-13	26.5751084444052	22.009084072291344	27.22889182920459	24.18691565409887
14-15	23.569890280492	23.756369477448906	24.291549611804893	28.3821906302542
16-17	23.971290206870364	26.903985949043115	25.3476783764922	23.777045467594327
18-19	23.991539610521983	26.213699101443588	26.591589930287423	23.203171357747006
20-21	24.189609440265354	25.565901117013688	26.68249795887742	23.561991483843535
22-23	24.193243343118173	25.362345481317522	26.35617326180103	24.088237913763273
24-25	23.874493936332055	25.605362436715986	26.4152917071884	24.10485191976356
26-27	23.7077294274087	25.80971425951252	26.474470254802924	24.008086058275858
28-29	23.782691991442544	26.106474853946533	26.309176378934957	23.80165677567597
30-31	24.027548743816084	25.810331839897177	26.181632099379183	23.980487316907556
32-33	23.751295144390248	25.838814982431124	26.09787227834962	24.31201759482901
34-35	24.162241658222065	25.84195459296269	26.19877802892136	23.797025719893885
36-37	24.129218740646387	25.730974321746913	26.340744742842375	23.799062194764325
38-39	23.913047184818684	25.91416832795274	26.348398165326365	23.824386321902207
40-41	24.03523903937548	25.6395951193203	26.51333446113819	23.81183138016603
42-43	23.8393663689354	25.67403419599838	26.2634593251855	24.223140109880724
44-45	23.652715750155163	25.77636651096758	26.536666470540226	24.03425126833703
46-47	23.8317904245764	25.70998731304585	26.318938335025184	24.139283927352572
48-49	23.556157005865785	25.944175852461854	26.342447912464813	24.157219229207556
50-51	23.65599622815702	25.89735014364587	26.453180708329032	23.99347291986808
52-53	23.907162327357767	25.68179919971451	26.429447317069467	23.981591155858258
54-55	23.230157030854944	25.650100298694216	26.768609166131384	24.35113350431945
56-57	23.37746992994648	25.80676389942133	26.521137067053395	24.294629103578792
58-59	23.424947595386648	25.56606664127908	26.68179797276441	24.327187790569866
60-61	23.369767445392498	25.71551320877365	26.798512665052716	24.116206680781136
62-63	23.4881498739421	25.56997494786658	26.76865442444062	24.1732207537507
64-65	23.208354252816182	25.481837776172767	26.685205520275844	24.62460245073521
66-67	23.28445092084686	25.577689590891428	26.527017815990828	24.61084167227088
68-69	23.445888671797153	25.474401543624747	26.65083461797907	24.428875166599035
70-71	23.273374208631033	25.425818091133973	26.613777781657888	24.687029918577107
72-73	23.372477874308878	25.562772769005072	26.451615597304922	24.613133759381128
74-75	23.354357700213914	25.707631598427632	26.434310400133626	24.50370030122483
76-77	23.398516213411853	25.450608741962377	26.51585735469651	24.635017689929256
78-79	23.380563919608406	25.48509713712107	26.553698484827198	24.580640458443323
80-81	23.52632614130319	25.608982342416773	26.34254435659023	24.5221471596898
82-83	23.55013438489649	25.651539403337516	26.367556408527808	24.430769803238185
84-85	23.516746120819924	25.64353687670001	26.329237103444296	24.51047989903577
86-87	23.49886540413961	25.75603806074963	26.29032628626297	24.454770248847794
88-89	23.50220125475698	25.526789843774328	26.25128251473895	24.71972638672974
90-91	23.619350585533276	25.45442531612113	26.338744234477613	24.587479863867976
92-93	23.871426236085007	25.27923968808164	26.36169990987093	24.487634165962426
94-95	23.86541014941538	25.062111630240185	26.47507645640262	24.597401763941818
96-97	23.930416626395434	24.893660209787647	26.412067660228395	24.763855503588523
98-99	23.770801668215704	25.008824302861086	26.3015490710036	24.91882495791961
100-101	24.197495185056557	24.9114340926668	26.272245437457762	24.618825284818882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	42.0
2	85.5
3	161.5
4	153.5
5	156.0
6	260.5
7	426.5
8	695.0
9	856.0
10	741.5
11	651.0
12	692.0
13	754.5
14	848.5
15	943.0
16	1033.5
17	1150.5
18	1251.0
19	1365.5
20	1534.0
21	1626.0
22	1684.0
23	1878.0
24	2171.5
25	2515.0
26	3066.5
27	3951.0
28	5114.5
29	6971.0
30	9883.0
31	13895.0
32	20939.0
33	26316.5
34	29949.0
35	35823.5
36	43262.5
37	51352.0
38	58390.0
39	65372.0
40	71139.0
41	76277.5
42	81981.5
43	87885.0
44	93574.5
45	100137.5
46	105375.5
47	109021.0
48	112931.5
49	115311.0
50	116577.0
51	117109.0
52	116818.5
53	115748.5
54	112338.5
55	106271.5
56	98298.0
57	88943.0
58	80089.5
59	69628.5
60	57452.5
61	47351.0
62	39096.5
63	30622.5
64	22551.5
65	16590.0
66	12446.0
67	9558.0
68	7365.0
69	5702.0
70	4450.0
71	3288.0
72	2434.5
73	1842.0
74	1381.0
75	1045.0
76	783.5
77	517.0
78	314.5
79	210.5
80	156.0
81	129.0
82	108.0
83	82.5
84	66.5
85	53.0
86	49.0
87	47.5
88	43.0
89	35.0
90	26.0
91	28.5
92	25.0
93	18.0
94	14.0
95	10.5
96	9.0
97	6.0
98	6.5
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04914055182907424
2	0.06918322871232811
3	0.06668263008417166
4	0.06581120935011715
5	0.06274229285192516
6	0.010229721660639972
7	0.013222862442827221
8	0.016860096811054767
9	0.005834730132365021
10-11	0.011385301329712265
12-13	0.021728686564359348
14-15	0.038039409434379744
16-17	0.026010014518627184
18-19	0.009320413068583085
20-21	0.019000760788188686
22-23	0.03534937151621145
24-25	0.032810885030052646
26-27	0.019133368291196984
28-29	0.01007817022863049
30-31	0.01000239451262575
32-33	0.006952421943434944
34-35	0.0040729447352548034
36-37	5.304300120331837E-4
38-39	0.0036940661552311007
40-41	8.524768050533309E-4
42-43	0.21918125854371198
44-45	0.18993183216588214
46-47	0.1957286744402448
48-49	0.2032494142537153
50-51	0.1936827301081168
52-53	0.20110875027658137
54-55	0.1983808245004107
56-57	0.2012034699215873
58-59	0.20970929404311944
60-61	0.21459682772542518
62-63	0.19932802095046998
64-65	0.1993090770214688
66-67	0.1966190391033005
68-69	0.19129579505396746
70-71	0.18379399916949815
72-73	0.19536873978922226
74-75	0.19409949654614286
76-77	0.18790483176275533
78-79	0.2050111996508255
80-81	0.21641544490953896
82-83	0.21601762240051406
84-85	0.21080804192518815
86-87	0.22927837270134366
88-89	0.22240172647391346
90-91	0.22891843805032114
92-93	0.2243908390190379
94-95	0.22749764337523223
96-97	0.2342037942416518
98-99	0.2279144098132583
100-101	0.23337026136559963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2639368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19538237509207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4150108435449	46.41806550830632
2	11.805455204997777	13.976568699437287
3	3.968773272371672	7.047991542542597
4	1.8259513630850748	4.323515565445663
5	1.0301765805400327	3.0490848299466036
6	0.6346321686579295	2.2540376334744034
7	0.4179686819031784	1.7319271162250307
8	0.3275147981923482	1.550989096999773
9	0.23252141098787465	1.2387774450440858
>10	1.2640876961523633	13.059102456206364
>50	0.056383057051027106	2.263168027141952
>100	0.01972669839044135	1.9863427000451603
>500	0.001091778933285541	0.4426276259960648
>1k	7.064451920978965E-4	0.6578017531887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.788785800237026E-5	0.0
2	0.0	0.0	0.0	3.788785800237026E-5	0.0
3	0.0	0.0	0.0	3.788785800237026E-5	0.0
4	0.0	0.0	0.0	3.788785800237026E-5	0.0
5	0.0	0.0	0.0	7.577571600474052E-5	0.0
6	0.0	0.0	0.0	7.577571600474052E-5	0.0
7	0.0	3.788785800237026E-5	0.0	7.577571600474052E-5	0.0
8	0.0	3.788785800237026E-5	0.0	7.577571600474052E-5	0.0
9	0.0	3.788785800237026E-5	0.0	7.577571600474052E-5	0.0
10-11	0.0	3.788785800237026E-5	0.0	1.326075030082959E-4	0.0
12-13	0.0	3.788785800237026E-5	0.0	1.8943929001185133E-4	3.788785800237026E-5
14-15	0.0	3.788785800237026E-5	0.0	1.8943929001185133E-4	5.6831787003555395E-5
16-17	0.0	3.788785800237026E-5	0.0	1.8943929001185133E-4	7.577571600474052E-5
18-19	0.0	3.788785800237026E-5	0.0	2.0838321901303647E-4	1.1366357400711079E-4
20-21	0.0	3.788785800237026E-5	0.0	3.031028640189621E-4	1.1366357400711079E-4
22-23	0.0	3.788785800237026E-5	0.0	4.925421540308134E-4	1.5155143200948107E-4
24-25	0.0	3.788785800237026E-5	0.0	8.903646630557011E-4	2.2732714801422158E-4
26-27	0.0	3.788785800237026E-5	0.0	0.0018375611131149576	2.2732714801422158E-4
28-29	0.0	3.788785800237026E-5	0.0	0.011176918110699229	2.2732714801422158E-4
30-31	0.0	3.788785800237026E-5	0.0	0.046621009271916605	2.462710770154067E-4
32-33	0.0	3.788785800237026E-5	0.0	0.1123753868350302	2.6521500601659186E-4
34-35	0.0	3.788785800237026E-5	0.0	0.2062993868229061	2.6521500601659186E-4
36-37	0.0	3.788785800237026E-5	0.0	0.3459161435616405	2.6521500601659186E-4
38-39	0.0	3.788785800237026E-5	0.0	0.555833820823773	2.6521500601659186E-4
40-41	0.0	3.788785800237026E-5	0.0	0.7840513335010503	2.6521500601659186E-4
42-43	0.0	3.788785800237026E-5	0.0	1.0340922523876928	2.6521500601659186E-4
44-45	0.0	3.788785800237026E-5	0.0	1.3449052955101373	2.6521500601659186E-4
46-47	0.0	3.788785800237026E-5	0.0	1.689836354763716	2.6521500601659186E-4
48-49	0.0	3.788785800237026E-5	0.0	2.035146292597319	2.6521500601659186E-4
50-51	0.0	3.788785800237026E-5	0.0	2.3930349992877082	2.6521500601659186E-4
52-53	0.0	3.788785800237026E-5	0.0	2.763313035544873	2.6521500601659186E-4
54-55	0.0	3.788785800237026E-5	0.0	3.2146332000691076	2.6521500601659186E-4
56-57	0.0	3.788785800237026E-5	0.0	3.719280524731678	2.6521500601659186E-4
58-59	0.0	3.788785800237026E-5	0.0	4.21818783890689	2.84158935017777E-4
60-61	0.0	3.788785800237026E-5	0.0	4.727324874742742	3.031028640189621E-4
62-63	0.0	3.788785800237026E-5	0.0	5.244588856120101	3.031028640189621E-4
64-65	0.0	3.788785800237026E-5	0.0	5.825883317521467	3.031028640189621E-4
66-67	0.0	3.788785800237026E-5	0.0	6.499756759951625	3.2204679302014726E-4
68-69	0.0	3.788785800237026E-5	0.0	7.191683766719912	3.4099072202133237E-4
70-71	0.0	3.788785800237026E-5	0.0	7.8826067452511355	3.7887858002370265E-4
72-73	0.0	3.788785800237026E-5	0.0	8.59029510094841	4.1676643802607293E-4
74-75	0.0	3.788785800237026E-5	0.0	9.342482745869464	4.1676643802607293E-4
76-77	1.894392900118513E-5	3.788785800237026E-5	0.0	10.196475065242891	4.3571036702725805E-4
78-79	3.788785800237026E-5	3.788785800237026E-5	0.0	11.112717135314213	4.5465429602844316E-4
80-81	3.788785800237026E-5	3.788785800237026E-5	0.0	12.014277660409613	4.925421540308134E-4
82-83	3.788785800237026E-5	3.788785800237026E-5	0.0	12.913110259728844	5.304300120331837E-4
84-85	7.577571600474052E-5	3.788785800237026E-5	0.0	13.853808942140695	5.304300120331837E-4
86-87	7.577571600474052E-5	5.6831787003555395E-5	0.0	14.899229664071097	5.493739410343688E-4
88-89	7.577571600474052E-5	7.577571600474052E-5	0.0	15.998394312577858	5.68317870035554E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1905	0.0	47.590366	1
GTATCAA	3250	0.0	40.455452	1
TCAACGC	3710	0.0	34.160007	4
CAACGCA	3825	0.0	33.132347	5
GTACATG	7030	0.0	31.126211	1
AACGCAG	4190	0.0	30.455935	6
ATCAACG	4195	0.0	30.21064	3
GAGTACA	5125	0.0	29.822403	1
TATCAAC	4300	0.0	29.694273	2
TACATGG	7370	0.0	29.239992	2
ACATGGG	7810	0.0	27.531368	3
CATGGGG	5890	0.0	25.78786	4
ACGCAGA	4925	0.0	25.23935	7
AGTACAT	5570	0.0	24.713354	2
CCCTATA	420	0.0	23.733341	2
CGCAGAG	5210	0.0	23.495329	8
ATGGGGG	3810	0.0	20.804817	5
ACCGCGT	140	4.891408E-4	20.333998	8
GCAGAGT	6175	0.0	18.363712	9
AGAGTAC	4975	0.0	17.404812	10-11
>>END_MODULE