##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2639368
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293552092773723	33.0	31.0	34.0	28.0	34.0
2	31.419016976791415	34.0	31.0	34.0	28.0	34.0
3	31.49794647809627	34.0	31.0	34.0	28.0	34.0
4	34.981500116694605	37.0	35.0	37.0	32.0	37.0
5	34.71559441502663	37.0	35.0	37.0	32.0	37.0
6	34.68955560573592	37.0	35.0	37.0	32.0	37.0
7	34.566251466260105	37.0	35.0	37.0	32.0	37.0
8	34.50843989924861	37.0	35.0	37.0	31.0	37.0
9	36.082260222901844	39.0	37.0	39.0	31.0	39.0
10-11	35.973778760673014	39.0	37.0	39.0	31.0	39.0
12-13	35.88759297680354	39.0	37.0	39.0	30.0	39.0
14-15	37.11537724182456	40.0	38.0	41.0	31.0	41.0
16-17	37.074209242515636	40.0	38.0	41.0	31.0	41.0
18-19	37.02573760081959	40.0	37.5	41.0	31.0	41.0
20-21	36.962106648258214	40.0	37.0	41.0	30.5	41.0
22-23	36.86959359210235	40.0	37.0	41.0	30.0	41.0
24-25	36.78115291236387	40.0	37.0	41.0	30.0	41.0
26-27	36.643485296480065	40.0	37.0	41.0	30.0	41.0
28-29	36.517740800070314	40.0	37.0	41.0	30.0	41.0
30-31	36.35141518727211	40.0	36.5	41.0	29.5	41.0
32-33	36.29240996329425	40.0	36.0	41.0	29.5	41.0
34-35	36.41782957132162	40.0	37.0	41.0	30.0	41.0
36-37	36.40889087842241	40.0	37.0	41.0	30.0	41.0
38-39	36.29544061305585	40.0	36.5	41.0	29.0	41.0
40-41	36.20151036157141	40.0	36.0	41.0	29.0	41.0
42-43	36.06659359361787	40.0	36.0	41.0	28.0	41.0
44-45	35.92286297325724	40.0	36.0	41.0	28.0	41.0
46-47	35.75621663974103	40.0	35.0	41.0	27.0	41.0
48-49	35.602257623794785	40.0	35.0	41.0	27.0	41.0
50-51	35.43023651874236	39.0	35.0	41.0	26.0	41.0
52-53	35.24668064476042	39.0	35.0	41.0	26.0	41.0
54-55	35.021829468266645	39.0	35.0	41.0	26.0	41.0
56-57	34.78928724603769	39.0	35.0	41.0	25.0	41.0
58-59	34.53662164578793	38.0	34.0	40.0	24.5	41.0
60-61	34.253567899588084	38.0	34.0	40.0	23.5	41.0
62-63	33.93361706287263	37.0	34.0	40.0	23.0	41.0
64-65	33.58624469948867	37.0	34.0	40.0	22.0	41.0
66-67	33.23147719454051	36.0	33.5	39.0	22.0	41.0
68-69	32.860007206270595	35.5	33.0	39.0	21.0	40.5
70-71	32.463338382521876	35.0	33.0	38.5	20.0	40.0
72-73	32.099703224408266	35.0	33.0	37.0	18.5	39.5
74-75	31.695494906356373	35.0	32.5	37.0	17.0	39.0
76-77	28.990659885245257	32.5	28.0	34.5	9.0	36.5
78-79	30.566003679668768	34.5	31.0	35.5	10.5	37.0
80-81	30.75301246358977	35.0	32.0	35.0	9.0	37.0
82-83	30.582064153236686	35.0	32.0	35.0	7.0	37.0
84-85	30.38315043601347	35.0	32.0	35.0	4.0	36.0
86-87	30.17850201260302	35.0	31.5	35.0	2.0	36.0
88-89	29.906410360358993	34.5	31.0	35.0	2.0	35.5
90-91	29.70464349798891	34.0	31.0	35.0	2.0	35.0
92-93	29.53645872799852	34.0	31.0	35.0	2.0	35.0
94-95	29.420301564616985	34.0	31.0	35.0	2.0	35.0
96-97	29.201190019731996	34.0	31.0	35.0	2.0	35.0
98-99	28.98506346973973	34.0	31.0	35.0	2.0	35.0
100-101	27.84050140033523	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51628.0
3	18101.0
4	10028.0
5	7041.0
6	6134.0
7	6400.0
8	6557.0
9	6961.0
10	7839.0
11	8486.0
12	8864.0
13	9611.0
14	10217.0
15	11034.0
16	11939.0
17	12512.0
18	11986.0
19	11001.0
20	10917.0
21	10868.0
22	11123.0
23	12506.0
24	14711.0
25	17401.0
26	20759.0
27	24852.0
28	29844.0
29	35803.0
30	43430.0
31	53882.0
32	67817.0
33	89596.0
34	128130.0
35	190288.0
36	333235.0
37	627763.0
38	626930.0
39	73173.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.017920923885406	18.585141085911445	11.291882152393125	27.10505583781002
2	16.341177776607463	20.97149956258385	35.08679935272261	27.60052330808608
3	18.803694731820475	23.653702821672013	28.67661017411558	28.86599227239193
4	13.27857398347368	15.535104789965384	34.46800223099261	36.718318995568325
5	14.022170986113194	37.27301873149702	34.26058563782134	14.444224644568443
6	34.8456633073507	34.681151672377084	16.13297632313086	14.340208697141355
7	29.77637070692681	31.4018734788025	20.244998044986527	18.57675776928416
8	28.009697776134285	33.63441551159217	19.48542226775501	18.870464444518536
9	27.135738555593612	14.363438520130579	18.53788482697373	39.96293809730208
10-11	26.303758435180946	24.782227579547275	27.470511810911695	21.44350217436008
12-13	26.428017722491603	22.15862721161737	27.24946748413819	24.163887581752835
14-15	23.458876633024982	23.885685835917254	24.20810874967391	28.447328781383852
16-17	23.910079988845816	27.13251808766341	25.228369064109284	23.729032859381487
18-19	23.99103257945571	26.39486051773706	26.45290550790882	23.161201394898406
20-21	24.1450539666302	25.6708201317553	26.58067999374849	23.603445907866007
22-23	24.114390263123596	25.509155979764852	26.249333173699156	24.127120583412392
24-25	23.805253288064723	25.75419425151547	26.379422408454484	24.061130051965318
26-27	23.692723071534104	25.984586405414934	26.411253617682156	23.911436905368806
28-29	23.752357442793826	26.258033776673084	26.224880387307365	23.76472839322572
30-31	24.0699769814057	25.940832227623563	26.042671839424226	23.946518951546516
32-33	23.77509086014141	25.984153210764266	25.98839710831431	24.252358820780017
34-35	24.081015948558417	26.011268950517408	26.181743087631816	23.72597201329236
36-37	24.050747538799687	25.829313584370777	26.29883895529847	23.821099921531065
38-39	23.863117988851876	26.0469172923215	26.295404809030043	23.794559909796586
40-41	24.02754223481146	25.760165983907807	26.383666528750894	23.828625252529847
42-43	23.81488813447318	25.80404857133709	26.12070613461982	24.260357159569914
44-45	23.61064958674993	25.86832151094214	26.4143769269563	24.10665197535163
46-47	23.73151779868571	25.853504116249184	26.220306475666238	24.19467160939887
48-49	23.503637133852134	26.060429696351044	26.259981372903408	24.175951796893415
50-51	23.602089110976017	25.975555302542773	26.357455155325958	24.06490043115525
52-53	23.88389812527469	25.746783262355454	26.283436642771623	24.08588196959823
54-55	23.17897104733852	25.776956502712032	26.58472187148161	24.459350578467838
56-57	23.245508505563333	25.89730425079239	26.371509062559795	24.485678181084474
58-59	23.233833249492932	25.758253542849076	26.484036692728242	24.52387651492975
60-61	23.22193219007029	25.90722617064222	26.544699074065303	24.326142565222185
62-63	23.2630916968743	25.72765965993506	26.50387796512797	24.505370678062672
64-65	22.961105543485345	25.684615816133917	26.407116335413996	24.947162304966746
66-67	23.00760436741698	25.82330730936338	26.194284886743883	24.974803436475753
68-69	23.125481188947525	25.671067859440328	26.275958615570826	24.927492336041325
70-71	22.95372909900612	25.54783712485128	26.276694240193756	25.22173953594884
72-73	23.00937598069165	25.605546299258197	26.13347109129472	25.251606628755436
74-75	22.82147486185961	25.771501769864553	26.060914417384843	25.346108950890994
76-77	22.818264301652633	25.444436871329977	26.248549743306228	25.48874908371116
78-79	22.721660680483424	25.470482909042374	26.27198178056594	25.535874629908268
80-81	22.77512879623551	25.545074843707138	26.032336636782766	25.64745972327458
82-83	22.698056331003578	25.510665052690463	26.114939714653655	25.676338901652308
84-85	22.53952850108227	25.37877610435297	26.135911325754275	25.945784068810486
86-87	22.45545266169387	25.551885784717292	26.067067015643484	25.92559453794535
88-89	22.373736270312527	25.282914916071153	26.064781612121546	26.278567201494766
90-91	22.302418242489967	25.171293126312342	26.137875767646268	26.38841286355142
92-93	22.33121664500572	24.94701961274137	26.300921776289616	26.420841965963287
94-95	22.213277672933515	24.67118045378736	26.433618740028557	26.681923133250567
96-97	22.06733701417537	24.555931563697257	26.461800859006296	26.914930563121086
98-99	21.895570384613563	24.61066460806926	26.308344423553258	27.18542058376392
100-101	21.849441058739508	24.58470124442811	26.336846173227276	27.229011523605113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	39.0
2	70.0
3	122.5
4	111.5
5	107.5
6	228.0
7	419.0
8	593.5
9	671.5
10	573.5
11	483.5
12	480.0
13	546.5
14	612.5
15	639.5
16	698.0
17	801.0
18	870.0
19	907.5
20	1039.5
21	1164.5
22	1257.0
23	1449.5
24	1707.0
25	2128.0
26	2754.5
27	3632.0
28	4987.5
29	6989.5
30	9954.5
31	14018.0
32	21283.5
33	26708.5
34	29961.0
35	35544.5
36	42747.0
37	50559.5
38	57620.5
39	64712.0
40	70479.0
41	75459.5
42	80856.5
43	86643.5
44	92372.0
45	98994.5
46	104077.5
47	107890.5
48	112661.5
49	115637.5
50	116830.5
51	117838.0
52	118305.0
53	118011.0
54	115265.5
55	109289.5
56	101056.0
57	91034.5
58	81627.5
59	71172.5
60	59159.5
61	48220.0
62	39602.5
63	30911.0
64	22065.0
65	16030.0
66	12174.0
67	9321.5
68	7056.5
69	5448.0
70	4149.5
71	2973.5
72	2143.5
73	1686.5
74	1272.5
75	900.0
76	622.0
77	372.5
78	208.0
79	126.0
80	73.5
81	44.0
82	30.0
83	18.5
84	10.5
85	8.5
86	8.5
87	6.0
88	2.5
89	2.0
90	2.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16049296649804046
2	1.8943929001185133E-4
3	0.009812955222613898
4	0.006971365872436129
5	0.02288426623343164
6	0.0015534021780971808
7	0.0
8	0.0
9	0.0
10-11	0.04681044856192847
12-13	3.7887858002370265E-4
14-15	0.00502014118531406
16-17	0.0
18-19	0.0013639628880853295
20-21	2.0838321901303647E-4
22-23	0.0
24-25	4.3571036702725805E-4
26-27	0.0028226454211765844
28-29	0.004565486889285617
30-31	0.015704517141982476
32-33	0.01077909560167434
34-35	0.009775067364611529
36-37	0.004054000806253618
38-39	0.0
40-41	0.0028794772081801403
42-43	0.009074141991567678
44-45	0.006327272286395835
46-47	0.002727925776170659
48-49	0.007653347316478793
50-51	0.0024058789831505117
52-53	0.0018186171841137726
54-55	0.004224496167264284
56-57	0.008070113754504865
58-59	0.01612128358000855
60-61	0.007463908026466942
62-63	0.02078149011430009
64-65	0.04167664380260729
66-67	0.0609047317388102
68-69	0.051944253321249634
70-71	0.06872857441629966
72-73	0.03500838079419013
74-75	0.02834011778577296
76-77	0.21908653889870605
78-79	0.08274708187717666
80-81	0.06711834045119892
82-83	0.028415893501777697
84-85	0.05296722548731363
86-87	0.0663984711491539
88-89	0.28512507539683746
90-91	0.28878125369406615
92-93	0.051660094386231856
94-95	0.010172889873636416
96-97	0.014719432833920848
98-99	0.001155579669072293
100-101	1.1366357400711079E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2639368.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.64304170553438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94142056552444	45.70721976812644
2	12.056826847010862	14.14097999251329
3	4.0020704295796765	7.0408074933098055
4	1.8568702387282385	4.355700754060226
5	1.0572918615296996	3.1001405365304127
6	0.6730765022319853	2.368275203484331
7	0.4496404381221926	1.8457798075706147
8	0.3290904467163031	1.5439091833341683
9	0.25763549935164076	1.3597676399974
>10	1.2980596696260063	13.267452253495044
>50	0.05683229193833803	2.2645673464476497
>100	0.01956630520681045	1.9854297085459227
>500	8.418303055917783E-4	0.3491800522851386
>1k	7.77074128219784E-4	0.6707902602996128
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1366357400711079E-4	0.0
5	0.0	0.0	0.0	1.1366357400711079E-4	0.0
6	0.0	0.0	0.0	1.1366357400711079E-4	0.0
7	0.0	0.0	0.0	1.1366357400711079E-4	0.0
8	0.0	0.0	0.0	1.1366357400711079E-4	0.0
9	0.0	0.0	0.0	1.1366357400711079E-4	0.0
10-11	0.0	0.0	0.0	1.1366357400711079E-4	0.0
12-13	0.0	0.0	0.0	1.1366357400711079E-4	0.0
14-15	0.0	0.0	0.0	1.1366357400711079E-4	1.894392900118513E-5
16-17	0.0	0.0	0.0	1.5155143200948107E-4	5.6831787003555395E-5
18-19	0.0	0.0	0.0	2.0838321901303647E-4	1.1366357400711079E-4
20-21	0.0	0.0	0.0	3.031028640189621E-4	1.326075030082959E-4
22-23	0.0	0.0	0.0	5.304300120331837E-4	1.7049536101066619E-4
24-25	0.0	0.0	0.0	9.471964500592567E-4	1.8943929001185133E-4
26-27	0.0	0.0	0.0	0.0019512246871220685	1.8943929001185133E-4
28-29	0.0	0.0	0.0	0.011271637755705152	1.8943929001185133E-4
30-31	0.0	0.0	0.0	0.04677256070392609	1.8943929001185133E-4
32-33	3.788785800237026E-5	0.0	0.0	0.11320891971108235	1.8943929001185133E-4
34-35	3.788785800237026E-5	0.0	0.0	0.20713291969895825	1.8943929001185133E-4
36-37	3.788785800237026E-5	0.0	0.0	0.34676862036669387	1.8943929001185133E-4
38-39	3.788785800237026E-5	0.0	0.0	0.5550760636637255	1.8943929001185133E-4
40-41	5.6831787003555395E-5	0.0	0.0	0.7823274359619424	1.8943929001185133E-4
42-43	1.1366357400711079E-4	0.0	0.0	1.0315916537595364	1.8943929001185133E-4
44-45	1.1366357400711079E-4	0.0	0.0	1.341703771508937	1.8943929001185133E-4
46-47	1.5155143200948104E-4	0.0	0.0	1.6853087557324329	1.8943929001185133E-4
48-49	1.5155143200948104E-4	0.0	0.0	2.0295010017549657	2.6521500601659186E-4
50-51	1.5155143200948104E-4	0.0	0.0	2.3848891098171987	2.6521500601659186E-4
52-53	1.5155143200948104E-4	0.0	0.0	2.7526097156592035	2.84158935017777E-4
54-55	1.5155143200948104E-4	0.0	0.0	3.200860963685246	3.031028640189621E-4
56-57	1.5155143200948104E-4	0.0	0.0	3.7019847175535965	3.031028640189621E-4
58-59	1.5155143200948104E-4	0.0	0.0	4.19740634879259	3.031028640189621E-4
60-61	1.5155143200948104E-4	0.0	0.0	4.702981925976218	3.031028640189621E-4
62-63	1.5155143200948104E-4	0.0	0.0	5.217442205861403	3.4099072202133237E-4
64-65	1.5155143200948104E-4	0.0	0.0	5.797183265084671	3.4099072202133237E-4
66-67	1.5155143200948104E-4	0.0	0.0	6.466548052412547	3.4099072202133237E-4
68-69	1.5155143200948104E-4	0.0	0.0	7.154383170516578	3.4099072202133237E-4
70-71	1.5155143200948104E-4	0.0	0.0	7.842900270064652	3.4099072202133237E-4
72-73	1.5155143200948104E-4	0.0	0.0	8.55083489683894	3.7887858002370265E-4
74-75	1.5155143200948104E-4	0.0	0.0	9.302946766043991	3.7887858002370265E-4
76-77	1.5155143200948104E-4	0.0	0.0	10.15517730002031	3.7887858002370265E-4
78-79	1.5155143200948104E-4	0.0	0.0	11.071362538304625	3.7887858002370265E-4
80-81	1.5155143200948104E-4	0.0	0.0	11.974305970217113	3.7887858002370265E-4
82-83	1.5155143200948104E-4	0.0	0.0	12.87482457921745	3.7887858002370265E-4
84-85	1.5155143200948104E-4	0.0	0.0	13.818630065985493	3.7887858002370265E-4
86-87	1.5155143200948104E-4	0.0	0.0	14.864675937572933	3.7887858002370265E-4
88-89	1.5155143200948104E-4	0.0	0.0	15.967989306530956	3.7887858002370265E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1855	0.0	51.75908	1
GTATCAA	3445	0.0	41.253525	1
TCAACGC	4100	0.0	33.57251	4
CAACGCA	4140	0.0	33.38683	5
GTACATG	7265	0.0	32.777905	1
AACGCAG	4405	0.0	31.15734	6
ATCAACG	4455	0.0	30.897844	3
TACATGG	7690	0.0	30.614925	2
TATCAAC	4680	0.0	29.615215	2
ACATGGG	8010	0.0	28.38448	3
CATGGGG	5940	0.0	26.44912	4
GAGTACA	5175	0.0	25.07445	1
ATGGGGG	3680	0.0	23.233103	5
ACGCAGA	5845	0.0	23.155838	7
CGCAGAG	6185	0.0	21.499012	8
AGTACAT	5625	0.0	21.433304	2
AGAGTAC	5770	0.0	18.85134	5
TATACCG	210	1.9577916E-5	18.094776	5
GCAGAGT	7135	0.0	17.305317	9
CAGAGTA	6410	0.0	17.253117	4
>>END_MODULE