FastQCFastQC Report
Mon 15 Aug 2016
SRR2921663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382149
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.046.0696561
GTATCAA35250.038.9151461
TCAACGC38400.033.8692634
CAACGCA39700.032.7572825
GTACATG86300.031.7904741
ATCAACG42350.030.8209763
AACGCAG44100.029.4732826
TACATGG91900.028.8211632
ACATGGG90700.027.9448033
TATCAAC48350.027.4887082
CATGGGG74650.024.5439194
GAGTACA60850.024.4933951
ACGCAGA54750.023.6566317
CGCAGAG57700.022.2008068
AGTACAT63300.021.596412
CCCTATA6900.018.5740872
GCAGAGT68350.018.4647399
GTATAGG11900.018.348091
ATGGGGG42250.017.6368395
AGAGTAC54850.017.30041910-11
Produced by FastQC (version 0.11.5)