##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921663_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3382149
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.503830257034803	31.0	31.0	34.0	27.0	34.0
2	30.541410505569093	33.0	31.0	34.0	26.0	34.0
3	30.608084090913795	33.0	31.0	34.0	26.0	34.0
4	34.07073165611568	37.0	35.0	37.0	30.0	37.0
5	34.0739071519321	37.0	35.0	37.0	30.0	37.0
6	34.121519779288256	37.0	35.0	37.0	30.0	37.0
7	34.02938782413194	37.0	35.0	37.0	30.0	37.0
8	34.00173233053896	37.0	35.0	37.0	30.0	37.0
9	35.57366514603585	39.0	37.0	39.0	30.0	39.0
10-11	35.473167947361276	39.0	37.0	39.0	30.0	39.0
12-13	35.369367227759625	39.0	35.0	39.0	30.0	39.0
14-15	36.52963441291321	40.0	37.0	41.0	29.0	41.0
16-17	36.46565098107742	40.0	37.0	41.0	29.0	41.0
18-19	36.428287754324245	40.0	37.0	41.0	29.0	41.0
20-21	36.34951210014698	40.0	37.0	41.0	28.0	41.0
22-23	36.251626406760906	40.0	37.0	41.0	27.5	41.0
24-25	36.143289222325805	40.0	36.0	41.0	27.0	41.0
26-27	36.001532014112925	40.0	36.0	41.0	27.0	41.0
28-29	35.863243754192965	40.0	36.0	41.0	27.0	41.0
30-31	35.72343841149518	40.0	36.0	41.0	26.5	41.0
32-33	35.55221073938493	40.0	35.5	41.0	25.5	41.0
34-35	35.382880677344495	39.0	35.0	41.0	25.0	41.0
36-37	35.35547665108781	39.0	35.0	41.0	25.0	41.0
38-39	35.544401503304556	40.0	35.5	41.0	25.5	41.0
40-41	35.60981701279275	40.0	36.0	41.0	26.0	41.0
42-43	35.451073858662056	40.0	35.0	41.0	25.0	41.0
44-45	35.33425833693312	40.0	35.0	41.0	24.0	41.0
46-47	35.1852227681276	40.0	35.0	41.0	24.0	41.0
48-49	35.0230369803341	39.5	35.0	41.0	23.0	41.0
50-51	33.30721635859331	37.0	32.5	39.5	20.0	40.0
52-53	33.43217004927932	37.5	33.5	39.5	21.0	40.0
54-55	34.350703945923144	39.0	34.0	40.5	22.0	41.0
56-57	34.36877204404655	39.0	34.0	41.0	22.0	41.0
58-59	34.17559294401282	38.5	34.0	41.0	21.0	41.0
60-61	33.922026202866874	38.0	34.0	40.0	20.0	41.0
62-63	33.623150399346684	37.0	34.0	40.0	19.5	41.0
64-65	33.331965120401264	37.0	34.0	40.0	18.0	41.0
66-67	32.98660984480577	36.0	33.0	39.5	16.5	41.0
68-69	32.62027663476683	36.0	33.0	39.0	13.0	41.0
70-71	32.21312618101686	35.0	33.0	39.0	9.5	40.5
72-73	31.850502446817096	35.0	32.5	37.5	7.0	40.0
74-75	31.44716850144686	35.0	32.0	37.0	3.5	39.0
76-77	31.066262604042578	35.0	32.0	36.5	2.0	39.0
78-79	30.719826063251503	35.0	32.0	36.0	2.0	38.5
80-81	30.399796992976952	35.0	31.0	36.0	2.0	37.0
82-83	30.080492314206147	35.0	31.0	35.0	2.0	37.0
84-85	29.801945745146057	35.0	31.0	35.0	2.0	36.0
86-87	29.557893960319312	35.0	31.0	35.0	2.0	36.0
88-89	29.319685945237776	35.0	31.0	35.0	2.0	36.0
90-91	29.075736018726555	35.0	30.5	35.0	2.0	35.5
92-93	28.857720934234415	34.0	30.0	35.0	2.0	35.0
94-95	28.605618203101045	34.0	30.0	35.0	2.0	35.0
96-97	28.34500623715868	34.0	30.0	35.0	2.0	35.0
98-99	28.048002616088173	34.0	29.5	35.0	2.0	35.0
100-101	26.94366170739373	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107535.0
3	19192.0
4	13280.0
5	10099.0
6	9155.0
7	9585.0
8	10029.0
9	10850.0
10	11518.0
11	12301.0
12	12157.0
13	12555.0
14	12936.0
15	13919.0
16	14116.0
17	14493.0
18	14165.0
19	13659.0
20	13890.0
21	14947.0
22	15877.0
23	18486.0
24	21973.0
25	25753.0
26	30848.0
27	36340.0
28	43215.0
29	50985.0
30	61038.0
31	74734.0
32	92706.0
33	118818.0
34	164515.0
35	238785.0
36	402845.0
37	730906.0
38	769973.0
39	133957.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.660546925150854	17.324502888097534	11.111558129064207	25.9033920576874
2	16.805680399980826	19.394827209726603	37.63673300072609	26.162759389566485
3	19.74483321582517	22.90811551915482	28.795318924455614	28.5517323405644
4	13.284140701971353	14.528256669556288	36.041817681535896	36.14578494693645
5	14.211869027624221	36.943161340350606	34.52259898622532	14.32237064579986
6	34.68665107159216	34.753537796174086	16.409454388251117	14.150356743982634
7	30.03044588315239	30.999592517220435	20.576963679622725	18.392997920004447
8	27.6047411567585	34.02314037464861	19.685020975141516	18.68709749345137
9	27.084121832598452	14.609580502620972	18.705646916337074	39.6006507484435
10-11	26.14239571391101	24.632325870981457	27.775405554137805	21.449872860969723
12-13	26.28150682703369	22.08550575055968	27.517840190926268	24.115147231480357
14-15	23.44789206143005	23.698137115537172	24.34780409632268	28.5061667267101
16-17	23.92968513400969	26.965561236561175	25.298573189544214	23.80618043988492
18-19	23.982072921568808	26.26476963603678	26.44417052813263	23.30898691426178
20-21	24.15014917238419	25.701358616858457	26.500917273019102	23.647574937738256
22-23	24.15744859952126	25.490860411759886	26.162786742546697	24.188904246172157
24-25	23.906587405129127	25.710749611880125	26.27973084045367	24.10293214253708
26-27	23.743171439112047	25.898471895791115	26.24268533503476	24.11567133006208
28-29	23.98945995268452	25.895326661183343	26.22445918459528	23.89075420153686
30-31	24.10913252271007	25.796685749432246	25.997867974072676	24.09631375378501
32-33	23.80980683155192	25.91382215799734	26.002293999143077	24.27407701130766
34-35	24.219532759731806	25.857530438299985	26.02425306356705	23.898683738401157
36-37	24.31561199274717	25.642602178658258	26.01823851998483	24.023547308609743
38-39	24.05670712289648	25.895540195191675	26.066237552886463	23.98151512902538
40-41	24.1606179117655	25.67998412820906	26.239030117669458	23.92036784235598
42-43	24.0363929883285	25.56074348182188	26.10176678570878	24.30109674414084
44-45	23.811373870287692	25.706125274702813	26.298714619677412	24.18378623533209
46-47	24.10018776577686	25.67925114102223	26.057345291724264	24.16321580147665
48-49	23.838790842435767	25.87778507766479	26.03007043197637	24.253353647923078
50-51	23.85038521449423	25.864784765141508	26.140312520552378	24.144517499811883
52-53	24.063661992611838	25.66898851008225	26.18397052556045	24.083378971745464
54-55	23.640026207081867	25.621970102418263	26.440297047170525	24.29770664332935
56-57	23.67192051632216	25.754099334451148	26.305837243686515	24.268142905540177
58-59	23.72391795967316	25.524337064885756	26.451509200981615	24.30023577445947
60-61	23.69596641800277	25.71913537675966	26.410784782438963	24.17411342279861
62-63	23.729615476513718	25.567094484467034	26.502824640326267	24.20046539869298
64-65	23.57033705282019	25.457667530965438	26.40984293305398	24.562152483160393
66-67	23.53178094772549	25.5939652490779	26.30877364499548	24.56548015820113
68-69	23.699360576439545	25.475020355528923	26.38504617746348	24.440572890568056
70-71	23.6216423329718	25.36206544407975	26.347054666872243	24.669237556076208
72-73	23.669725368581776	25.543740701394103	26.16745459791806	24.61907933210606
74-75	23.67270397677407	25.65575112111775	26.207085538229098	24.464459363879083
76-77	23.686176567560466	25.41396120967883	26.35953194248726	24.540330280273444
78-79	23.64676994640959	25.42385310228102	26.341430534311566	24.587946416997827
80-81	23.805809983889308	25.628878589607552	26.054062238779036	24.511249187724108
82-83	23.82651380104871	25.548548424565958	26.180005072869157	24.444932701516173
84-85	23.723395886570696	25.560067300371834	26.208404487264293	24.508132325793177
86-87	23.727500080755075	25.65030060149935	26.174912228866692	24.447287088878884
88-89	23.715472177496963	25.421518392243502	26.203637125474	24.65937230478554
90-91	23.822879930322667	25.495245615420643	26.16281387139654	24.519060582860153
92-93	24.00491265065329	25.320909169303313	26.255832994769975	24.418345185273427
94-95	23.90686887464359	25.16753488312824	26.312641199082577	24.612955043145597
96-97	24.01003486716248	25.011891584314665	26.29886477972044	24.67920876880241
98-99	23.9096255284382	25.042077246104068	26.236924344822572	24.81137288063516
100-101	24.203834114646465	24.922174929183928	26.262760673978153	24.611230282191453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	40.0
2	72.5
3	128.5
4	121.5
5	115.0
6	199.0
7	359.0
8	567.0
9	653.5
10	588.5
11	551.0
12	595.5
13	671.0
14	784.5
15	895.5
16	968.5
17	1062.0
18	1184.5
19	1292.5
20	1372.5
21	1445.5
22	1601.5
23	1842.5
24	2033.5
25	2367.5
26	2980.0
27	3805.5
28	4962.0
29	6882.5
30	9713.5
31	13987.0
32	21824.0
33	29128.0
34	35422.5
35	44415.5
36	57772.5
37	70381.5
38	79815.0
39	89879.5
40	97155.0
41	102172.5
42	107409.0
43	115685.0
44	124312.0
45	132068.5
46	137216.5
47	140339.0
48	145450.0
49	148426.0
50	147782.5
51	146148.5
52	145160.0
53	143968.5
54	141857.5
55	135236.5
56	124205.5
57	112066.5
58	102171.5
59	91120.0
60	76026.5
61	62736.0
62	51710.5
63	40169.0
64	29329.0
65	21628.0
66	16336.0
67	12635.0
68	9689.5
69	7246.5
70	5650.0
71	4269.0
72	3078.0
73	2360.5
74	1812.5
75	1367.0
76	1023.5
77	674.0
78	432.5
79	308.5
80	203.5
81	154.5
82	137.5
83	117.5
84	96.0
85	78.0
86	69.5
87	54.5
88	43.5
89	43.0
90	33.0
91	28.0
92	25.0
93	22.5
94	24.5
95	24.0
96	17.5
97	11.5
98	11.5
99	11.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05094394126338018
2	0.07057642936488014
3	0.06628921434271524
4	0.06620051334225666
5	0.06232723632223181
6	0.009224904047692755
7	0.012152037062826032
8	0.015581809080557953
9	0.0058246990301136945
10-11	0.012359006063896063
12-13	0.02185001311296457
14-15	0.03734312119306393
16-17	0.02663986713772811
18-19	0.009978862551590719
20-21	0.019469869600659228
22-23	0.03542126618312795
24-25	0.0327011021690647
26-27	0.019854240602646425
28-29	0.011250243558163758
30-31	0.011501563059463081
32-33	0.008337894043106912
34-35	0.005455111528202926
36-37	6.356905032865199E-4
38-39	0.004139380021400595
40-41	0.001419216007337347
42-43	0.2197715121362187
44-45	0.1908697694867967
46-47	0.19712319001912693
48-49	0.2042044865557372
50-51	0.19594051001301244
52-53	0.20358357955252712
54-55	0.1988528595280693
56-57	0.20148432254167398
58-59	0.20997005158554516
60-61	0.21422769960755722
62-63	0.19997640553387802
64-65	0.20025729203533021
66-67	0.19806933402401844
68-69	0.1930872944982613
70-71	0.1855181424591288
72-73	0.19641358201545825
74-75	0.19548222151064307
76-77	0.18952447097984151
78-79	0.20730902157178763
80-81	0.21796792512689417
82-83	0.2168148121209326
84-85	0.21082749458997815
86-87	0.22908511718437008
88-89	0.22274299565158126
90-91	0.22877466368276503
92-93	0.22459093316113513
94-95	0.22773981867741486
96-97	0.23399323920974502
98-99	0.22768068467710914
100-101	0.2340228062098979
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3382149.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.27066792281276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1204956470508	43.17771972873686
2	11.654284409473343	12.882801669480306
3	3.9803235935535213	6.599854306939003
4	1.8807589524676969	4.158032140187971
5	1.0762247511393013	2.974183041526607
6	0.6580482770367864	2.1822460678367595
7	0.4848720879167564	1.8759442909401545
8	0.3195407541585097	1.4128984728720115
9	0.25473074545585717	1.2671224597639106
>10	1.4289240480769902	14.470758668661155
>50	0.09676179971444097	3.706587386966065
>100	0.04277927334997189	4.167370882971887
>500	0.001557479944434077	0.5719767217493094
>1k	6.981806616746606E-4	0.5525041613679595
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.956700015286139E-5
2	0.0	0.0	0.0	0.0	2.956700015286139E-5
3	0.0	0.0	0.0	0.0	2.956700015286139E-5
4	0.0	0.0	0.0	5.913400030572278E-5	2.956700015286139E-5
5	0.0	2.956700015286139E-5	0.0	5.913400030572278E-5	2.956700015286139E-5
6	2.956700015286139E-5	2.956700015286139E-5	0.0	5.913400030572278E-5	5.913400030572278E-5
7	2.956700015286139E-5	2.956700015286139E-5	0.0	5.913400030572278E-5	5.913400030572278E-5
8	2.956700015286139E-5	2.956700015286139E-5	0.0	5.913400030572278E-5	8.870100045858417E-5
9	2.956700015286139E-5	2.956700015286139E-5	0.0	5.913400030572278E-5	1.1826800061144556E-4
10-11	2.956700015286139E-5	2.956700015286139E-5	0.0	8.870100045858417E-5	1.1826800061144556E-4
12-13	2.956700015286139E-5	2.956700015286139E-5	0.0	1.4783500076430696E-4	1.4783500076430696E-4
14-15	2.956700015286139E-5	2.956700015286139E-5	0.0	1.4783500076430696E-4	2.513195012993218E-4
16-17	2.956700015286139E-5	2.956700015286139E-5	0.0	1.4783500076430696E-4	4.139380021400595E-4
18-19	2.956700015286139E-5	2.956700015286139E-5	0.0	1.7740200091716837E-4	4.2872150221649015E-4
20-21	2.956700015286139E-5	2.956700015286139E-5	0.0	2.808865014521832E-4	4.4350500229292086E-4
22-23	2.956700015286139E-5	2.956700015286139E-5	0.0	3.9915450206362877E-4	4.7307200244578224E-4
24-25	2.956700015286139E-5	2.956700015286139E-5	0.0	6.061235031336585E-4	5.026390025986437E-4
26-27	2.956700015286139E-5	2.956700015286139E-5	0.0	0.0011235460058087328	5.32206002751505E-4
28-29	2.956700015286139E-5	2.956700015286139E-5	0.0	0.006977812036075288	5.32206002751505E-4
30-31	2.956700015286139E-5	2.956700015286139E-5	0.0	0.028088650145218323	5.469895028279357E-4
32-33	2.956700015286139E-5	2.956700015286139E-5	0.0	0.06757537884936471	5.617730029043664E-4
34-35	2.956700015286139E-5	4.4350500229292085E-5	0.0	0.11927327861664284	5.617730029043664E-4
36-37	2.956700015286139E-5	5.913400030572278E-5	0.0	0.20685073306941829	5.617730029043664E-4
38-39	2.956700015286139E-5	5.913400030572278E-5	0.0	0.35428657933166163	6.061235031336585E-4
40-41	2.956700015286139E-5	5.913400030572278E-5	0.0	0.5211775116944878	6.80041003515812E-4
42-43	5.913400030572278E-5	5.913400030572278E-5	0.0	0.6930948340833003	7.096080036686735E-4
44-45	5.913400030572278E-5	5.913400030572278E-5	0.0	0.8959688056321586	7.687420039743961E-4
46-47	5.913400030572278E-5	5.913400030572278E-5	0.0	1.1384329903856987	7.983090041272575E-4
48-49	5.913400030572278E-5	5.913400030572278E-5	0.0	1.3872540801721036	9.165770047387032E-4
50-51	5.913400030572278E-5	5.913400030572278E-5	0.0	1.6603792440841607	9.165770047387032E-4
52-53	5.913400030572278E-5	5.913400030572278E-5	0.0	1.9352045105050073	9.461440048915645E-4
54-55	5.913400030572278E-5	8.870100045858417E-5	0.0	2.2376453550686266	0.0010052780051972873
56-57	5.913400030572278E-5	8.870100045858417E-5	0.0	2.5839931948592447	0.0010348450053501487
58-59	5.913400030572278E-5	8.870100045858417E-5	0.0	2.951407522258777	0.0010939790056558713
60-61	5.913400030572278E-5	1.0348450053501487E-4	0.0	3.34652612880154	0.0011383295058851636
62-63	5.913400030572278E-5	1.1826800061144556E-4	0.0	3.7436848583548508	0.0011531130059615942
64-65	5.913400030572278E-5	1.4783500076430696E-4	0.0	4.165694651536642	0.001167896506038025
66-67	5.913400030572278E-5	1.7740200091716834E-4	0.0	4.646661043023238	0.0011826800061144557
68-69	5.913400030572278E-5	1.7740200091716834E-4	0.0	5.15998851617714	0.0011826800061144557
70-71	5.913400030572278E-5	1.7740200091716834E-4	0.0	5.7123148625326685	0.0011974635061908863
72-73	5.913400030572278E-5	1.7740200091716834E-4	0.0	6.277739389955912	0.0013009480067259012
74-75	5.913400030572278E-5	1.7740200091716834E-4	0.0	6.852403604926927	0.0013009480067259012
76-77	5.913400030572278E-5	1.7740200091716834E-4	0.0	7.483851243691511	0.0013009480067259012
78-79	8.870100045858417E-5	1.7740200091716834E-4	0.0	8.175911232769462	0.0013009480067259012
80-81	8.870100045858417E-5	2.0696900107002974E-4	0.0	8.904841271038029	0.0013452985069551933
82-83	1.0348450053501487E-4	2.0696900107002974E-4	0.0	9.642020502349245	0.001507917007795931
84-85	1.1826800061144556E-4	2.0696900107002974E-4	0.0	10.388468988208384	0.001507917007795931
86-87	1.1826800061144556E-4	2.0696900107002974E-4	0.0	11.177006098785121	0.001507917007795931
88-89	1.1826800061144556E-4	2.661030013757525E-4	0.0	12.034936958720625	0.0015374840079487922
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2040	0.0	46.069656	1
GTATCAA	3525	0.0	38.915146	1
TCAACGC	3840	0.0	33.869263	4
CAACGCA	3970	0.0	32.757282	5
GTACATG	8630	0.0	31.790474	1
ATCAACG	4235	0.0	30.820976	3
AACGCAG	4410	0.0	29.473282	6
TACATGG	9190	0.0	28.821163	2
ACATGGG	9070	0.0	27.944803	3
TATCAAC	4835	0.0	27.488708	2
CATGGGG	7465	0.0	24.543919	4
GAGTACA	6085	0.0	24.493395	1
ACGCAGA	5475	0.0	23.656631	7
CGCAGAG	5770	0.0	22.200806	8
AGTACAT	6330	0.0	21.59641	2
CCCTATA	690	0.0	18.574087	2
GCAGAGT	6835	0.0	18.464739	9
GTATAGG	1190	0.0	18.34809	1
ATGGGGG	4225	0.0	17.636839	5
AGAGTAC	5485	0.0	17.300419	10-11
>>END_MODULE