FastQCFastQC Report
Mon 15 Aug 2016
SRR2921663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3382149
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.043.9565931
GTATCAA36700.039.1201131
TCAACGC40500.033.284924
CAACGCA42600.031.5560265
GTACATG85500.031.1373161
ATCAACG45100.029.8895653
AACGCAG45900.029.1773266
TACATGG91150.028.8494342
ACATGGG89400.028.404953
TATCAAC50100.027.1912022
CATGGGG74250.024.7399444
ACGCAGA58450.022.586567
GAGTACA63050.022.4693451
CGCAGAG62500.020.9709878
AGTACAT67400.019.9301592
TATACCG2902.4429028E-919.6557125
TACACCG4001.8189894E-1219.000525
ACTATAC8800.018.8783113
GTATAGT8450.018.565921
ATGGGGG39600.018.472735
Produced by FastQC (version 0.11.5)