FastQCFastQC Report
Mon 15 Aug 2016
SRR2921662_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921662_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596418
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.047.7619061
GTATCAA41050.038.6151351
TCAACGC45400.033.7667354
CAACGCA47150.032.5116545
ATCAACG48650.031.5105533
GTACATG81300.031.4646191
AACGCAG51400.029.9926556
TATCAAC53950.028.9440232
TACATGG87450.028.3854892
ACATGGG91600.026.4765723
TAAGGCG14300.025.2229445
CATGGGG75500.024.2651374
ACGCAGA64150.023.516877
GTATTAA22550.023.3614981
CGCAGAG68250.021.965428
GAGTACA61850.021.331891
TATTAAG21400.020.6264112
AGTACAT66800.018.3314882
ATGGGGG46150.018.3048635
GTATAGT7950.017.909291
Produced by FastQC (version 0.11.5)