##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921662_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3596418
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.508880224712478	31.0	31.0	34.0	27.0	34.0
2	30.549057979356125	31.0	31.0	34.0	26.0	34.0
3	30.614042639092563	33.0	31.0	34.0	26.0	34.0
4	34.0850738151127	37.0	35.0	37.0	30.0	37.0
5	34.09124328707063	37.0	35.0	37.0	30.0	37.0
6	34.138301498880274	37.0	35.0	37.0	30.0	37.0
7	34.04875907083103	37.0	35.0	37.0	30.0	37.0
8	34.01971517215185	37.0	35.0	37.0	30.0	37.0
9	35.58923573399977	39.0	37.0	39.0	30.0	39.0
10-11	35.49221127799939	39.0	36.0	39.0	30.0	39.0
12-13	35.38279185011309	39.0	35.0	39.0	30.0	39.0
14-15	36.533346652141105	40.0	37.0	41.0	29.0	41.0
16-17	36.475602391045754	40.0	37.0	41.0	29.0	41.0
18-19	36.43961436073337	40.0	37.0	41.0	29.0	41.0
20-21	36.36251194938964	40.0	37.0	41.0	28.0	41.0
22-23	36.265239051745375	40.0	36.5	41.0	28.0	41.0
24-25	36.157054185581316	40.0	36.0	41.0	27.0	41.0
26-27	36.010086702936086	40.0	36.0	41.0	27.0	41.0
28-29	35.874975044613834	40.0	36.0	41.0	27.0	41.0
30-31	35.72933652317389	40.0	36.0	41.0	27.0	41.0
32-33	35.55228229866495	39.5	35.5	41.0	26.0	41.0
34-35	35.38142229295927	39.0	35.0	41.0	25.0	41.0
36-37	35.35174846194185	39.0	35.0	41.0	25.0	41.0
38-39	35.53231965805977	40.0	35.0	41.0	25.5	41.0
40-41	35.59307149502644	40.0	35.0	41.0	26.0	41.0
42-43	35.43132069186619	40.0	35.0	41.0	25.0	41.0
44-45	35.31156180955607	40.0	35.0	41.0	24.0	41.0
46-47	35.15756372034619	40.0	35.0	41.0	24.0	41.0
48-49	34.99462868331768	39.5	35.0	41.0	23.0	41.0
50-51	33.2833002170493	37.0	32.5	39.5	20.0	40.0
52-53	33.40240455920308	37.5	33.5	39.5	21.0	40.0
54-55	34.32505301107936	39.0	34.0	40.5	22.0	41.0
56-57	34.341318917878844	39.0	34.0	41.0	22.0	41.0
58-59	34.1536038080112	38.0	34.0	41.0	21.0	41.0
60-61	33.90383612249744	38.0	34.0	40.0	20.0	41.0
62-63	33.611466603715144	37.0	34.0	40.0	18.5	41.0
64-65	33.32137838260179	37.0	33.5	40.0	18.0	41.0
66-67	32.98213222156045	36.0	33.0	39.5	16.0	41.0
68-69	32.617883961208065	36.0	33.0	39.0	13.0	41.0
70-71	32.20395126484185	35.0	33.0	39.0	9.0	40.5
72-73	31.838706457369526	35.0	32.0	37.5	7.0	40.0
74-75	31.433238711406737	35.0	32.0	37.0	2.0	39.0
76-77	31.043079808854255	35.0	32.0	36.5	2.0	39.0
78-79	30.686786686085988	35.0	31.5	36.0	2.0	38.5
80-81	30.353600165498	35.0	31.0	36.0	2.0	37.0
82-83	30.020947926520222	35.0	31.0	35.0	2.0	37.0
84-85	29.735543671508708	35.0	31.0	35.0	2.0	36.5
86-87	29.472214436697847	35.0	31.0	35.0	2.0	36.0
88-89	29.218446103873355	35.0	30.5	35.0	2.0	36.0
90-91	28.96394231704991	34.5	30.0	35.0	2.0	35.5
92-93	28.736396047400497	34.0	30.0	35.0	2.0	35.0
94-95	28.46990741899301	34.0	30.0	35.0	2.0	35.0
96-97	28.19263778570789	34.0	29.5	35.0	2.0	35.0
98-99	27.882765295913877	34.0	29.0	35.0	2.0	35.0
100-101	26.77443278284115	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109329.0
3	19706.0
4	13681.0
5	10694.0
6	9880.0
7	10102.0
8	10810.0
9	11612.0
10	12655.0
11	13393.0
12	13557.0
13	13916.0
14	14598.0
15	15557.0
16	15585.0
17	16062.0
18	15575.0
19	15215.0
20	15798.0
21	16297.0
22	17938.0
23	20619.0
24	23952.0
25	28509.0
26	33685.0
27	40450.0
28	47848.0
29	56639.0
30	67132.0
31	81232.0
32	100445.0
33	128551.0
34	176809.0
35	254044.0
36	422387.0
37	760657.0
38	815203.0
39	146275.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64390767465682	17.394351268296674	11.451142382291197	25.510598674755308
2	16.613005416767184	19.49398985653511	37.90412541901548	25.98887930768223
3	19.44026617740597	23.030283300417306	29.30992650516389	28.219524017012827
4	13.454693682213033	14.940400336117618	36.1041241186651	35.50078186300424
5	14.231554697368495	36.93339714090158	34.545983183737974	14.289064977991952
6	34.40338309138401	34.78583571027928	16.66704208369241	14.143739114644296
7	29.413286064132677	31.398486814551628	20.857052753473578	18.331174367842113
8	26.96676023153063	34.359237349484374	20.002096935720576	18.67190548326442
9	26.83622354746371	14.770457805246599	19.10669664681029	39.2866220004794
10-11	25.841594882875075	24.770020958443105	28.080197990753703	21.308186167928117
12-13	26.24592867169081	22.294539587731684	27.6106493238487	23.848882416728802
14-15	23.27346118347521	23.963876428466914	24.731594814708906	28.031067573348974
16-17	23.7678522805055	26.986568148435964	25.677134367745087	23.56844520331345
18-19	23.723284382974086	26.427924285266176	26.808316629699913	23.04047470205983
20-21	24.15339892634379	25.77834512881163	26.695298425948522	23.372957518896058
22-23	24.023896386682637	25.639867700337177	26.380962222607636	23.955273690372557
24-25	23.813522561314564	25.72024768234154	26.489961043561365	23.976268712782527
26-27	23.664264167299727	25.94234863676899	26.460825159871128	23.932562036060155
28-29	23.807223635366064	26.169444891700532	26.28041538364422	23.742916089289185
30-31	23.995242481073372	25.931703963746305	26.096581972373688	23.976471582806635
32-33	23.74933973803085	26.031569385868426	26.03700581503266	24.18208506106806
34-35	24.137557558612496	25.987900369429003	26.10126923353388	23.77327283842462
36-37	24.184233711124367	25.77744312106437	26.15375118678085	23.884571981030415
38-39	23.879914299975113	25.954149652486674	26.229032065212106	23.936903982326108
40-41	24.135257364119717	25.739512317060502	26.287633917889785	23.837596400929993
42-43	23.907844378867757	25.749666387587943	26.1416818747847	24.200807358759597
44-45	23.778241838460445	25.80631859329245	26.383637635166572	24.03180193308053
46-47	23.92704986778642	25.78805703156129	26.18096403066129	24.103929069991
48-49	23.704848473279945	25.889320546075524	26.203020126513653	24.20281085413088
50-51	23.777926476572762	25.985992127694463	26.263471880951055	23.972609514781716
52-53	23.975027910940796	25.74643392710873	26.28373956587811	23.994798596072364
54-55	23.412458744874503	25.644627662783332	26.576792526408227	24.36612106593394
56-57	23.541751887300332	25.794572109752657	26.429881484338164	24.233794518608843
58-59	23.584695600800362	25.58993194700673	26.476613402148047	24.348759050044865
60-61	23.493987739567604	25.670268137911123	26.631304554541757	24.204439567979517
62-63	23.602891972620636	25.638343478662485	26.5986887119693	24.16007583674758
64-65	23.430171044319714	25.479690663994592	26.51037877303851	24.579759518647183
66-67	23.390954287995175	25.567138571023545	26.44516906984245	24.596738071138834
68-69	23.63667266184545	25.476769959278293	26.47059970827488	24.41595767060138
70-71	23.46152694224984	25.394217113515943	26.422175927183524	24.72208001705069
72-73	23.535947455634624	25.564652144631868	26.328648599246957	24.570751800486555
74-75	23.556583659244186	25.696742414701262	26.281710131972996	24.464963794081555
76-77	23.55687112382596	25.426113587475825	26.42175197529112	24.5952633134071
78-79	23.524717015334726	25.49225795421723	26.432918363004827	24.550106667443224
80-81	23.709405687419093	25.598959893759783	26.25154852365949	24.44008589516164
82-83	23.670907207432926	25.609221817525107	26.297851005380778	24.42201996966119
84-85	23.611658602347187	25.627104473868467	26.3273869863133	24.433849937471045
86-87	23.653711074689767	25.72167218561506	26.22187566199507	24.402741077700103
88-89	23.604144987896124	25.48747533201529	26.24410187632067	24.664277803767913
90-91	23.68911915943076	25.468090099853274	26.290826008232653	24.55196473248331
92-93	23.841989895610084	25.30240633204347	26.386441002980476	24.46916276936597
94-95	23.80692300595448	25.198531434596745	26.40266698701877	24.591878572430005
96-97	23.890279145385986	25.007794495699265	26.369479305387433	24.732447053527316
98-99	23.814633832262018	25.04781239229756	26.29792370949744	24.839630065942984
100-101	24.12799226198316	24.935787143212547	26.348393337110355	24.587827257693938
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	44.5
2	95.0
3	173.5
4	164.5
5	170.5
6	276.5
7	464.0
8	732.5
9	820.5
10	685.0
11	632.5
12	698.5
13	784.0
14	857.5
15	975.5
16	1096.0
17	1229.0
18	1371.5
19	1482.5
20	1640.0
21	1788.5
22	1957.5
23	2184.5
24	2424.0
25	2864.5
26	3523.0
27	4510.5
28	6147.0
29	8568.5
30	12462.5
31	18353.0
32	26983.0
33	33958.0
34	39564.5
35	47917.0
36	58870.5
37	69619.0
38	79676.5
39	90395.0
40	99670.0
41	108070.0
42	116836.0
43	125918.0
44	134684.5
45	144147.5
46	150207.0
47	154476.0
48	159807.5
49	162300.5
50	161793.5
51	160545.5
52	158491.5
53	155917.0
54	151790.5
55	142713.0
56	130442.0
57	116929.5
58	104954.5
59	91565.0
60	75019.5
61	61283.0
62	50736.0
63	39193.0
64	28205.5
65	20878.0
66	15731.0
67	11972.5
68	9166.0
69	6919.0
70	5271.5
71	3892.0
72	2762.5
73	2061.0
74	1547.0
75	1150.0
76	854.0
77	569.5
78	367.5
79	278.0
80	206.5
81	149.5
82	124.0
83	98.5
84	72.5
85	61.0
86	57.5
87	52.0
88	47.0
89	43.5
90	36.0
91	32.0
92	24.0
93	23.0
94	24.0
95	16.0
96	11.5
97	8.5
98	8.0
99	9.5
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05316400930036497
2	0.07165462968987475
3	0.06740039672807777
4	0.0677896729468043
5	0.06334080187564405
6	0.011594870229211398
7	0.015571048749060871
8	0.01932478371535233
9	0.007229415490635404
10-11	0.013694181265915142
12-13	0.023551211232954568
14-15	0.0411520574082323
16-17	0.02805569319250432
18-19	0.010955345012732112
20-21	0.021146040310108558
22-23	0.0388581082621653
24-25	0.03471509707714732
26-27	0.02189678730336685
28-29	0.011594870229211398
30-31	0.011845119226964162
32-33	0.008550174089886103
34-35	0.005352548007489675
36-37	8.202606037451709E-4
38-39	0.0042542329617969886
40-41	0.0012373422666664444
42-43	0.2341774510081976
44-45	0.2037582950591394
46-47	0.2093193838980897
48-49	0.21670172933179627
50-51	0.20874937229209733
52-53	0.21609000955951171
54-55	0.21191919293029898
56-57	0.21393508763441846
58-59	0.223500160437413
60-61	0.22824098867261816
62-63	0.21289238347711528
64-65	0.21407411485539224
66-67	0.21105722416026165
68-69	0.20639981225764079
70-71	0.19798866538872842
72-73	0.20929157845389498
74-75	0.20859644234902616
76-77	0.2019648439085779
78-79	0.22045546429808768
80-81	0.2314247120329172
82-83	0.23009005071156913
84-85	0.22468189181568993
86-87	0.24407618914152915
88-89	0.23686067637299113
90-91	0.2434088584808551
92-93	0.23833436491531296
94-95	0.24187955905014377
96-97	0.2488726282651238
98-99	0.24183785088385165
100-101	0.2484555466022025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3596418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.63338609650546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93641739664754	47.07245855689193
2	11.666553305843909	13.914321554057038
3	3.794927988061575	6.7891321776153175
4	1.7367461261017532	4.14272209157744
5	0.9688439589356713	2.8887722935238878
6	0.6039858183840621	2.1610631702706358
7	0.4203243497868666	1.7545754965621978
8	0.3158462832316739	1.50679866840805
9	0.24136190287798648	1.2953904788979196
>10	1.2341246396605257	13.08124099497471
>50	0.061575433864498264	2.479265770965537
>100	0.017374007206920165	1.7133658778623229
>500	0.0011231938066174987	0.4500766804008929
>1k	7.955955902744026E-4	0.7508161879921585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5610888389503114E-5
2	0.0	0.0	0.0	2.7805444194751557E-5	5.5610888389503114E-5
3	0.0	0.0	0.0	2.7805444194751557E-5	5.5610888389503114E-5
4	0.0	0.0	0.0	5.5610888389503114E-5	8.341633258425467E-5
5	0.0	0.0	0.0	5.5610888389503114E-5	8.341633258425467E-5
6	0.0	0.0	0.0	5.5610888389503114E-5	8.341633258425467E-5
7	0.0	2.7805444194751557E-5	0.0	5.5610888389503114E-5	8.341633258425467E-5
8	0.0	2.7805444194751557E-5	0.0	5.5610888389503114E-5	8.341633258425467E-5
9	0.0	2.7805444194751557E-5	0.0	5.5610888389503114E-5	8.341633258425467E-5
10-11	0.0	2.7805444194751557E-5	0.0	9.731905468163045E-5	8.341633258425467E-5
12-13	0.0	4.1708166292127334E-5	0.0	1.6683266516850933E-4	1.5292994307113355E-4
14-15	0.0	5.5610888389503114E-5	0.0	1.6683266516850933E-4	1.6683266516850933E-4
16-17	0.0	5.5610888389503114E-5	0.0	2.2244355355801243E-4	1.8073538726588511E-4
18-19	0.0	5.5610888389503114E-5	0.0	2.7805444194751555E-4	1.946381093632609E-4
20-21	0.0	5.5610888389503114E-5	0.0	3.197626082396429E-4	2.641517198501398E-4
22-23	0.0	5.5610888389503114E-5	0.0	5.283034397002796E-4	3.3366533033701867E-4
24-25	0.0	5.5610888389503114E-5	0.0	7.090388269661647E-4	3.6147077453177023E-4
26-27	0.0	5.5610888389503114E-5	0.0	0.0012095368224716925	3.892762187265218E-4
28-29	0.0	5.5610888389503114E-5	0.0	0.006214516777526972	3.892762187265218E-4
30-31	0.0	5.5610888389503114E-5	0.0	0.027583000641193546	3.892762187265218E-4
32-33	0.0	5.5610888389503114E-5	0.0	0.07636765248088515	4.448871071160249E-4
34-35	0.0	6.951361048687889E-5	0.0	0.14399049276252093	4.448871071160249E-4
36-37	0.0	8.341633258425467E-5	0.0	0.2570613315804781	4.448871071160249E-4
38-39	0.0	8.341633258425467E-5	0.0	0.43254148989355523	4.448871071160249E-4
40-41	0.0	8.341633258425467E-5	0.0	0.623328545235843	4.448871071160249E-4
42-43	0.0	8.341633258425467E-5	0.0	0.8258216925841212	4.448871071160249E-4
44-45	0.0	8.341633258425467E-5	0.0	1.074179920131642	4.448871071160249E-4
46-47	0.0	8.341633258425467E-5	0.0	1.3609513688342123	4.5878982921340066E-4
48-49	0.0	8.341633258425467E-5	0.0	1.6504060429015759	4.865952734081522E-4
50-51	0.0	8.341633258425467E-5	0.0	1.9581288938048913	5.561088838950311E-4
52-53	0.0	8.341633258425467E-5	0.0	2.2690632735127005	6.395252164792858E-4
54-55	0.0	1.1122177677900623E-4	0.0	2.6237078114946595	6.673306606740373E-4
56-57	0.0	1.1122177677900623E-4	0.0	3.02936143685189	6.673306606740373E-4
58-59	0.0	1.1122177677900623E-4	0.0	3.4504470837372074	6.673306606740373E-4
60-61	0.0	1.3902722097375777E-4	0.0	3.8830163790749577	6.673306606740373E-4
62-63	0.0	1.3902722097375777E-4	0.0	4.319992837317575	6.673306606740373E-4
64-65	0.0	1.3902722097375777E-4	0.0	4.801499714438088	6.951361048687888E-4
66-67	0.0	1.3902722097375777E-4	0.0	5.35892935693237	6.951361048687888E-4
68-69	0.0	1.3902722097375777E-4	0.0	5.947779151366721	6.951361048687888E-4
70-71	0.0	1.3902722097375777E-4	0.0	6.545888158717924	6.951361048687888E-4
72-73	0.0	1.3902722097375777E-4	0.0	7.15606472884965	7.785524374530435E-4
74-75	0.0	1.3902722097375777E-4	0.0	7.799566123848785	8.619687700372982E-4
76-77	0.0	1.3902722097375777E-4	0.0	8.526887030372999	8.619687700372982E-4
78-79	0.0	1.3902722097375777E-4	0.0	9.308929051072484	8.619687700372982E-4
80-81	0.0	1.3902722097375777E-4	0.0	10.102176665782453	8.75871492134674E-4
82-83	0.0	1.3902722097375777E-4	0.0	10.917418386850471	9.175796584268013E-4
84-85	0.0	1.3902722097375777E-4	0.0	11.758268922021855	9.175796584268013E-4
86-87	0.0	1.3902722097375777E-4	0.0	12.658609205047913	9.314823805241771E-4
88-89	0.0	1.3902722097375777E-4	0.0	13.623736173047739	9.453851026215529E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2355	0.0	47.761906	1
GTATCAA	4105	0.0	38.615135	1
TCAACGC	4540	0.0	33.766735	4
CAACGCA	4715	0.0	32.511654	5
ATCAACG	4865	0.0	31.510553	3
GTACATG	8130	0.0	31.464619	1
AACGCAG	5140	0.0	29.992655	6
TATCAAC	5395	0.0	28.944023	2
TACATGG	8745	0.0	28.385489	2
ACATGGG	9160	0.0	26.476572	3
TAAGGCG	1430	0.0	25.222944	5
CATGGGG	7550	0.0	24.265137	4
ACGCAGA	6415	0.0	23.51687	7
GTATTAA	2255	0.0	23.361498	1
CGCAGAG	6825	0.0	21.96542	8
GAGTACA	6185	0.0	21.33189	1
TATTAAG	2140	0.0	20.626411	2
AGTACAT	6680	0.0	18.331488	2
ATGGGGG	4615	0.0	18.304863	5
GTATAGT	795	0.0	17.90929	1
>>END_MODULE