FastQCFastQC Report
Mon 15 Aug 2016
SRR2921662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596418
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.048.6881941
GTATCAA41950.038.197631
TCAACGC50600.030.5838554
GTACATG84400.030.4221461
ATCAACG52800.029.308713
CAACGCA52650.029.2342535
TATCAAC54950.028.1615732
TACATGG90800.027.6029242
AACGCAG57150.026.9274716
ACATGGG95100.025.5565663
CATGGGG75200.023.4196934
ACGCAGA70650.021.1092227
CGCAGAG72200.020.3929128
ATGGGGG43700.019.7849675
TAAGGTG16600.018.3154375
GAGTACA64150.018.1596681
TATACTG11850.017.2383425
GTAAGGT15600.016.7364464
TGTACCG7100.016.7273465
AGAGTAC70850.016.49111410-11
Produced by FastQC (version 0.11.5)