##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3596418
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.427809559400494	33.0	31.0	34.0	29.0	34.0
2	31.56107743871819	34.0	31.0	34.0	29.0	34.0
3	31.63946154201208	34.0	31.0	34.0	28.0	34.0
4	35.116534006892415	37.0	35.0	37.0	32.0	37.0
5	34.86929244598375	37.0	35.0	37.0	32.0	37.0
6	34.84651784080716	37.0	35.0	37.0	32.0	37.0
7	34.729426612813086	37.0	35.0	37.0	32.0	37.0
8	34.678923862576596	37.0	35.0	37.0	32.0	37.0
9	36.27541375891234	39.0	37.0	39.0	32.0	39.0
10-11	36.174249489353016	39.0	37.0	39.0	32.0	39.0
12-13	36.0940224412179	39.0	37.0	39.0	32.0	39.0
14-15	37.34499910744524	40.0	38.0	41.0	32.0	41.0
16-17	37.31008214284324	40.0	38.0	41.0	31.5	41.0
18-19	37.26559287602275	40.0	38.0	41.0	31.0	41.0
20-21	37.20563113075288	40.0	38.0	41.0	31.0	41.0
22-23	37.114897934556	40.0	38.0	41.0	31.0	41.0
24-25	37.02973305661355	40.0	37.0	41.0	31.0	41.0
26-27	36.905120177910355	40.0	37.0	41.0	30.0	41.0
28-29	36.78295181483354	40.0	37.0	41.0	30.0	41.0
30-31	36.629489119451634	40.0	37.0	41.0	30.0	41.0
32-33	36.57370875687977	40.0	37.0	41.0	30.0	41.0
34-35	36.694608496565195	40.0	37.0	41.0	30.0	41.0
36-37	36.68688789790286	40.0	37.0	41.0	30.0	41.0
38-39	36.58111932483932	40.0	37.0	41.0	30.0	41.0
40-41	36.49746887041495	40.0	37.0	41.0	30.0	41.0
42-43	36.370332925705526	40.0	36.5	41.0	29.5	41.0
44-45	36.238203401273154	40.0	36.0	41.0	29.0	41.0
46-47	36.08257438373404	40.0	36.0	41.0	28.0	41.0
48-49	35.93855719774509	40.0	35.5	41.0	28.0	41.0
50-51	35.777797936724824	39.5	35.0	41.0	28.0	41.0
52-53	35.59775657334603	39.0	35.0	41.0	27.5	41.0
54-55	35.37902323923415	39.0	35.0	41.0	27.0	41.0
56-57	35.149653766608886	39.0	35.0	41.0	26.0	41.0
58-59	34.90452875611233	38.5	35.0	40.5	26.0	41.0
60-61	34.627870008436176	38.0	34.5	40.0	26.0	41.0
62-63	34.31832464969311	37.5	34.0	40.0	26.0	41.0
64-65	33.97802507939845	37.0	34.0	40.0	25.0	41.0
66-67	33.625835762138884	36.0	34.0	39.0	24.0	41.0
68-69	33.25850693662416	36.0	34.0	39.0	23.0	41.0
70-71	32.859665228012986	35.0	33.0	38.5	22.0	40.0
72-73	32.49454165227735	35.0	33.0	37.5	22.0	40.0
74-75	32.08641987666617	35.0	33.0	37.0	20.5	39.0
76-77	29.365138034566616	33.0	29.0	34.5	14.0	36.5
78-79	30.93411444387165	34.5	31.5	36.0	17.5	37.0
80-81	31.112305354939274	35.0	32.0	35.5	18.0	37.0
82-83	30.931159837371517	35.0	32.0	35.0	18.0	37.0
84-85	30.72961777524192	35.0	32.0	35.0	16.0	36.0
86-87	30.51591500209375	35.0	32.0	35.0	11.0	36.0
88-89	30.241419100894277	35.0	32.0	35.0	7.0	36.0
90-91	30.03570191229162	34.5	31.0	35.0	2.0	35.0
92-93	29.875803229769176	34.0	31.0	35.0	2.0	35.0
94-95	29.75956145809525	34.0	31.0	35.0	2.0	35.0
96-97	29.546570226264024	34.0	31.0	35.0	2.0	35.0
98-99	29.331390427920226	34.0	31.0	35.0	2.0	35.0
100-101	28.207474075594106	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61801.0
3	21370.0
4	12094.0
5	8591.0
6	7624.0
7	7626.0
8	8151.0
9	8938.0
10	9714.0
11	10601.0
12	11404.0
13	12167.0
14	12985.0
15	14068.0
16	14664.0
17	15607.0
18	15183.0
19	14218.0
20	13621.0
21	13629.0
22	14439.0
23	16156.0
24	19195.0
25	22778.0
26	27273.0
27	32947.0
28	39611.0
29	47669.0
30	57655.0
31	71675.0
32	90737.0
33	120032.0
34	169654.0
35	253005.0
36	445424.0
37	853926.0
38	906971.0
39	113214.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22480818578161	18.87186751721224	12.051701197914506	26.851623099091643
2	15.779378819261103	20.835422222555888	36.701220578638306	26.68397837954471
3	18.23296525323501	23.96806827052038	29.066889281480467	28.732077194764138
4	13.032790600478341	15.970822396969231	35.132750474600435	35.863636527951996
5	14.03188636519133	37.194724098168884	34.59534898726693	14.178040549372858
6	34.50275876700646	35.29884302236182	16.35139820812918	13.847000002502535
7	29.269742635517517	31.533912780414507	20.838323253393586	18.35802133067439
8	26.961020660001147	34.800042709162284	19.730576367930535	18.508360262906038
9	26.690788849890158	14.747750049841327	18.95099367063794	39.61046742963058
10-11	25.829526278277505	24.992975656546605	28.02320787438638	21.154290190789517
12-13	26.136123339749457	22.447956745724724	27.607438812203565	23.808481102322247
14-15	23.196980828192284	24.132074306952468	24.64843621827153	28.022508646583717
16-17	23.714432487329404	27.2410113675623	25.55404093100982	23.490515214098476
18-19	23.688076277124647	26.606276902201113	26.706544951006016	22.99910186966822
20-21	24.085890640125758	25.930132280505795	26.62660449912489	23.357372580243553
22-23	23.954004227818377	25.773648359791373	26.35489344604735	23.917453966342894
24-25	23.730055385987697	25.894902592789393	26.42928464237958	23.94575737884333
26-27	23.61557943067136	26.124306441109297	26.41356158235701	23.846552545862338
28-29	23.71608288333431	26.32258975869467	26.266641992552444	23.69468536541858
30-31	23.960353057521743	26.03948008058317	26.089538649108345	23.91062821278674
32-33	23.716659167522717	26.196855031443565	25.95799014784901	24.128495653184707
34-35	24.02658003724687	26.133389061812174	26.134890727409598	23.705140173531365
36-37	24.131255503992627	25.8968014764256	26.179039683804227	23.79290333577754
38-39	23.854874489005446	26.07602898217059	26.217058195126373	23.852038333697585
40-41	24.030860097803448	25.87721889850789	26.25665111686487	23.835269886823784
42-43	23.86769680445851	25.883539851702757	26.04376950122334	24.204993842615387
44-45	23.734818060034662	25.926106158700673	26.29483151878838	24.044244262476287
46-47	23.87376452710359	25.902407894791722	26.15454238320271	24.069285194901976
48-49	23.615737262179994	26.00642306586409	26.156794217739098	24.221045454216817
50-51	23.66968507664572	26.09174462947466	26.226841080165432	24.011729213714187
52-53	23.924011739696834	25.86623363442606	26.141082126436704	24.068672499440407
54-55	23.35434357197229	25.8239074594805	26.435014129252245	24.386734839294963
56-57	23.402555799490283	25.926696300333553	26.27426389721187	24.396484002964293
58-59	23.40089093889531	25.75080936613523	26.33770496954909	24.510594725420376
60-61	23.37805004369247	25.824383386990142	26.43609185637726	24.361474712940133
62-63	23.451202774467447	25.81002852061313	26.35816383430511	24.380604870614313
64-65	23.18824313022875	25.708681058718007	26.266293422991925	24.83678238806132
66-67	23.22097555424666	25.80099120723009	26.147312770035526	24.83072046848773
68-69	23.33416143055139	25.725897224325696	26.154319301304795	24.78562204381812
70-71	23.19492995196571	25.61100211442139	26.12302376449369	25.071044169119205
72-73	23.221055664868153	25.79955351505371	25.988027349845378	24.99136347023276
74-75	23.17216339923998	25.907559549751785	25.885170072416745	25.035106978591486
76-77	23.155320459403644	25.649154294855535	26.041559252312485	25.153965993428333
78-79	23.07077061196978	25.810072143859703	26.036840998673505	25.08231624549701
80-81	23.225325270024797	25.891690135338983	25.774772254290546	25.10821234034567
82-83	23.08965615834258	25.905836579013936	25.815916612790247	25.18859064985324
84-85	22.985992518938776	25.878668598669062	25.828133669824833	25.307205212567325
86-87	22.95016716308024	26.030959567973415	25.70723456991766	25.311638699028688
88-89	22.798095942988724	25.83540933628236	25.743603201167264	25.62289151956165
90-91	22.751482631631056	25.792435754953374	25.761800046574084	25.694281566841482
92-93	22.795652377728516	25.599987536411827	25.908030125716706	25.69632996014295
94-95	22.642130400475523	25.502862525766208	25.956250456231338	25.89875661752693
96-97	22.612445402095872	25.390743844126284	25.926447105177825	26.07036364860002
98-99	22.390176883716766	25.5043691228999	25.84668613442317	26.258767858960162
100-101	22.321521918536497	25.483065325180014	25.895282181554485	26.300130574729007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	45.5
2	99.0
3	156.5
4	139.5
5	148.5
6	235.5
7	426.0
8	653.5
9	657.0
10	508.0
11	450.5
12	458.5
13	479.0
14	501.5
15	557.5
16	643.5
17	681.5
18	768.5
19	907.0
20	1009.0
21	1142.5
22	1378.5
23	1671.0
24	2033.5
25	2531.5
26	3276.5
27	4423.5
28	6054.0
29	8703.0
30	12918.5
31	18741.5
32	27453.0
33	34444.5
34	39910.5
35	48262.0
36	58903.0
37	69669.5
38	79667.5
39	89713.0
40	99443.0
41	108158.5
42	116356.0
43	125808.5
44	134740.5
45	143859.0
46	150709.5
47	155649.0
48	160662.5
49	163279.0
50	163217.5
51	162095.5
52	160389.0
53	158296.5
54	154069.0
55	144134.0
56	131437.5
57	117184.5
58	104939.0
59	91694.0
60	74813.0
61	61231.5
62	50360.0
63	38541.5
64	27327.0
65	19770.0
66	14837.5
67	11432.0
68	8736.0
69	6457.0
70	4795.5
71	3495.0
72	2417.0
73	1770.5
74	1342.0
75	928.0
76	629.5
77	399.0
78	213.5
79	118.0
80	73.5
81	49.5
82	31.0
83	22.0
84	18.5
85	14.5
86	8.0
87	4.0
88	3.5
89	3.5
90	3.5
91	1.5
92	0.5
93	0.5
94	1.0
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16947418236701073
2	2.50248997752764E-4
3	0.010315819796252828
4	0.006534279385766615
5	0.0250248997752764
6	0.001807353872658851
7	2.7805444194751557E-5
8	0.0
9	1.3902722097375777E-4
10-11	0.04938246888987877
12-13	2.9195716404489136E-4
14-15	0.005561088838950312
16-17	1.3902722097375778E-5
18-19	0.0016127157632955904
20-21	2.0854083146063665E-4
22-23	1.3902722097375778E-5
24-25	5.839143280897827E-4
26-27	0.003114209749812174
28-29	0.005741824226216196
30-31	0.01751742984269348
32-33	0.012568060776027703
34-35	0.011428037564042889
36-37	0.004587898292134007
38-39	0.0
40-41	0.0034339723580518173
42-43	0.009161893862170638
44-45	0.0068262365498115065
46-47	0.0034200696359544414
48-49	0.008786520365541492
50-51	0.002738836253183028
52-53	0.0020297974262168637
54-55	0.004323746572283867
56-57	0.00786894070711469
58-59	0.015251286140821227
60-61	0.0072433182127327805
62-63	0.02149360836254295
64-65	0.04024838047190288
66-67	0.0615890588913747
68-69	0.05093957376478485
70-71	0.07026435748013718
72-73	0.03403386369437591
74-75	0.02722152986666177
76-77	0.23045152148610087
78-79	0.08651663961196947
80-81	0.06791479744568067
82-83	0.027763736028459426
84-85	0.052121305143061784
86-87	0.06874896077152322
88-89	0.29476551390856126
90-91	0.2984358325422684
92-93	0.05415110256927865
94-95	0.010579971516102968
96-97	0.01623837940973491
98-99	0.0012234395445690684
100-101	2.7805444194751555E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3596418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.82755561776855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.27662081995341	46.04822264856789
2	11.966115176023818	14.078746120923311
3	3.948320424051223	6.968101180279346
4	1.7970727709047365	4.228695935183033
5	1.0059294515666681	2.9588185379794796
6	0.6589627349169077	2.325910016301676
7	0.42347704774132794	1.743848370519598
8	0.3114914875291159	1.465942624566442
9	0.23690730537189378	1.2543009914718684
>10	1.2919488199175952	13.48333420391736
>50	0.06311830905627494	2.5052503150050804
>100	0.01809211975750693	1.7402813600270708
>500	0.0011376779850051397	0.46310567124972474
>1k	8.058552244307468E-4	0.735442024008163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7805444194751557E-5
2	0.0	0.0	0.0	0.0	2.7805444194751557E-5
3	0.0	0.0	0.0	0.0	2.7805444194751557E-5
4	0.0	0.0	0.0	0.0	5.5610888389503114E-5
5	0.0	0.0	0.0	0.0	8.341633258425467E-5
6	0.0	0.0	0.0	0.0	1.1122177677900623E-4
7	0.0	0.0	0.0	5.5610888389503114E-5	1.1122177677900623E-4
8	0.0	0.0	0.0	5.5610888389503114E-5	1.1122177677900623E-4
9	0.0	0.0	0.0	5.5610888389503114E-5	1.1122177677900623E-4
10-11	0.0	0.0	0.0	5.5610888389503114E-5	1.1122177677900623E-4
12-13	0.0	0.0	0.0	8.341633258425467E-5	1.8073538726588511E-4
14-15	0.0	1.3902722097375778E-5	0.0	8.341633258425467E-5	2.2244355355801243E-4
16-17	0.0	2.7805444194751557E-5	0.0	1.25124498876382E-4	2.50248997752764E-4
18-19	0.0	2.7805444194751557E-5	0.0	1.946381093632609E-4	2.7805444194751555E-4
20-21	0.0	2.7805444194751557E-5	0.0	2.3634627565538824E-4	3.058598861422671E-4
22-23	0.0	2.7805444194751557E-5	0.0	4.1708166292127335E-4	3.3366533033701867E-4
24-25	0.0	2.7805444194751557E-5	0.0	5.978170501871585E-4	3.3366533033701867E-4
26-27	0.0	2.7805444194751557E-5	0.0	0.001126120489887438	3.6147077453177023E-4
28-29	0.0	2.7805444194751557E-5	0.0	0.006075489556553215	3.892762187265218E-4
30-31	0.0	2.7805444194751557E-5	0.0	0.02733275164344078	3.892762187265218E-4
32-33	0.0	2.7805444194751557E-5	0.0	0.07646497153556678	3.892762187265218E-4
34-35	0.0	2.7805444194751557E-5	0.0	0.14439367170334483	4.7269255131077647E-4
36-37	0.0	2.7805444194751557E-5	0.0	0.2578398840179312	4.7269255131077647E-4
38-39	0.0	2.7805444194751557E-5	0.0	0.4338066376044164	4.7269255131077647E-4
40-41	0.0	2.7805444194751557E-5	0.0	0.6244129575594383	4.7269255131077647E-4
42-43	0.0	2.7805444194751557E-5	0.0	0.8271702566275667	4.7269255131077647E-4
44-45	0.0	2.7805444194751557E-5	0.0	1.0749306671249004	5.00497995505528E-4
46-47	0.0	2.7805444194751557E-5	0.0	1.3610347851667965	5.978170501871585E-4
48-49	0.0	2.7805444194751557E-5	0.0	1.6504199456236734	6.117197722845342E-4
50-51	0.0	2.7805444194751557E-5	0.0	1.9581984074153782	6.117197722845342E-4
52-53	0.0	4.1708166292127334E-5	0.0	2.2679093475786187	6.534279385766615E-4
54-55	0.0	5.5610888389503114E-5	0.0	2.6220255821208767	6.673306606740373E-4
56-57	0.0	5.5610888389503114E-5	0.0	3.027331639425673	6.812333827714131E-4
58-59	0.0	5.5610888389503114E-5	0.0	3.4482643563679196	6.951361048687888E-4
60-61	0.0	5.5610888389503114E-5	0.0	3.8819180640292643	6.951361048687888E-4
62-63	0.0	5.5610888389503114E-5	0.0	4.318977938604467	7.229415490635405E-4
64-65	0.0	5.5610888389503114E-5	0.0	4.801166049107751	7.229415490635405E-4
66-67	0.0	5.5610888389503114E-5	0.0	5.358303734437988	7.229415490635405E-4
68-69	0.0	5.5610888389503114E-5	0.0	5.947542805091066	7.229415490635405E-4
70-71	0.0	5.5610888389503114E-5	0.0	6.546611100266988	7.507469932582921E-4
72-73	0.0	5.5610888389503114E-5	0.0	7.159359673986728	8.341633258425467E-4
74-75	0.0	5.5610888389503114E-5	0.0	7.8047796446353015	9.036769363294256E-4
76-77	0.0	5.5610888389503114E-5	0.0	8.533574239701837	9.175796584268013E-4
78-79	0.0	5.5610888389503114E-5	0.0	9.317089948943643	9.175796584268013E-4
80-81	0.0	5.5610888389503114E-5	0.0	10.11596816610305	9.314823805241771E-4
82-83	0.0	5.5610888389503114E-5	0.0	10.936534629734364	9.731905468163044E-4
84-85	0.0	5.5610888389503114E-5	0.0	11.782918448300503	0.0010288014352058076
86-87	0.0	5.5610888389503114E-5	0.0	12.686442454686858	0.0010288014352058076
88-89	0.0	5.5610888389503114E-5	0.0	13.657603204076946	0.0010288014352058076
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2295	0.0	48.688194	1
GTATCAA	4195	0.0	38.19763	1
TCAACGC	5060	0.0	30.583855	4
GTACATG	8440	0.0	30.422146	1
ATCAACG	5280	0.0	29.30871	3
CAACGCA	5265	0.0	29.234253	5
TATCAAC	5495	0.0	28.161573	2
TACATGG	9080	0.0	27.602924	2
AACGCAG	5715	0.0	26.927471	6
ACATGGG	9510	0.0	25.556566	3
CATGGGG	7520	0.0	23.419693	4
ACGCAGA	7065	0.0	21.109222	7
CGCAGAG	7220	0.0	20.392912	8
ATGGGGG	4370	0.0	19.784967	5
TAAGGTG	1660	0.0	18.315437	5
GAGTACA	6415	0.0	18.159668	1
TATACTG	1185	0.0	17.238342	5
GTAAGGT	1560	0.0	16.736446	4
TGTACCG	710	0.0	16.727346	5
AGAGTAC	7085	0.0	16.491114	10-11
>>END_MODULE