FastQCFastQC Report
Mon 15 Aug 2016
SRR2921661_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921661_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272403
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22810.10037832197897997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.055.1774561
GTATCAA33400.042.7788051
TCAACGC40500.034.22524
CAACGCA41750.033.4264225
ATCAACG43100.032.1598633
TATCAAC44900.031.1884572
GTACATG60900.031.178221
AACGCAG45000.030.8904846
TACATGG64800.028.6430952
ACATGGG67500.026.5122263
ACGCAGA54700.024.5480237
CATGGGG54500.023.6913934
GAGTACA47150.023.6589131
CGCAGAG57250.023.3727668
ATGGGGG35700.021.9388985
AGTACAT51200.020.0263582
ACCGTGC5250.019.8838238
GCAGAGT63700.019.8147359
TCTATAC5300.018.8084093
GTATATG6400.018.5425151
Produced by FastQC (version 0.11.5)