##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921661_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2272403
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.507953474801784	31.0	31.0	34.0	27.0	34.0
2	30.54815849125353	31.0	31.0	34.0	26.0	34.0
3	30.60603642927773	33.0	31.0	34.0	26.0	34.0
4	34.08157443904096	37.0	35.0	37.0	30.0	37.0
5	34.086695009643975	37.0	35.0	37.0	30.0	37.0
6	34.13448846881473	37.0	35.0	37.0	30.0	37.0
7	34.04299457446589	37.0	35.0	37.0	30.0	37.0
8	34.024943638958405	37.0	35.0	37.0	30.0	37.0
9	35.588383310530745	39.0	37.0	39.0	30.0	39.0
10-11	35.494080055342295	39.0	37.0	39.0	30.0	39.0
12-13	35.38366632151075	39.0	35.0	39.0	30.0	39.0
14-15	36.52665416301598	40.0	37.0	41.0	29.0	41.0
16-17	36.461863498684	40.0	37.0	41.0	29.0	41.0
18-19	36.43343544256895	40.0	37.0	41.0	29.0	41.0
20-21	36.35642225432725	40.0	37.0	41.0	28.0	41.0
22-23	36.256805461003175	40.0	36.5	41.0	28.0	41.0
24-25	36.13894256432508	40.0	36.0	41.0	27.0	41.0
26-27	35.99626958774478	40.0	36.0	41.0	27.0	41.0
28-29	35.85371388789753	40.0	36.0	41.0	27.0	41.0
30-31	35.69405030709781	40.0	36.0	41.0	26.5	41.0
32-33	35.501156044944494	39.5	35.0	41.0	25.5	41.0
34-35	35.32400788944567	39.0	35.0	41.0	25.0	41.0
36-37	35.287063738254176	39.0	35.0	41.0	25.0	41.0
38-39	35.45875819561935	39.5	35.0	41.0	25.5	41.0
40-41	35.50998282434938	40.0	35.0	41.0	25.0	41.0
42-43	35.34237655028619	40.0	35.0	41.0	24.5	41.0
44-45	35.21420892332918	40.0	35.0	41.0	24.0	41.0
46-47	35.040458492617724	40.0	35.0	41.0	23.5	41.0
48-49	34.86694327546655	39.0	35.0	41.0	23.0	41.0
50-51	33.148907346100145	37.0	32.5	39.5	18.5	40.0
52-53	33.268919729467	37.5	33.0	39.5	19.0	40.0
54-55	34.18706255008465	39.0	34.0	40.5	20.0	41.0
56-57	34.208911007422536	39.0	34.0	41.0	20.0	41.0
58-59	34.02016785754992	38.0	34.0	41.0	19.5	41.0
60-61	33.76371840734236	38.0	34.0	40.0	18.0	41.0
62-63	33.467944946384954	37.0	34.0	40.0	15.5	41.0
64-65	33.1768592542784	37.0	33.0	40.0	13.0	41.0
66-67	32.83213826948829	36.0	33.0	39.5	10.0	41.0
68-69	32.46494217794995	36.0	33.0	39.0	7.5	41.0
70-71	32.04661387086709	35.0	32.5	39.0	4.0	40.5
72-73	31.674383901095005	35.0	32.0	37.5	2.0	40.0
74-75	31.254425601444815	35.0	32.0	37.0	2.0	39.0
76-77	30.85205661143732	35.0	32.0	36.5	2.0	39.0
78-79	30.478260678233568	35.0	31.0	36.0	2.0	38.5
80-81	30.130597653673227	35.0	31.0	36.0	2.0	37.0
82-83	29.774698193938313	35.0	31.0	35.0	2.0	37.0
84-85	29.46591207633505	35.0	31.0	35.0	2.0	36.5
86-87	29.183244345303187	35.0	30.5	35.0	2.0	36.0
88-89	28.901822652055998	35.0	30.0	35.0	2.0	36.0
90-91	28.612077831264962	34.0	30.0	35.0	2.0	35.5
92-93	28.347698669646185	34.0	29.5	35.0	2.0	35.0
94-95	28.051290418116857	34.0	29.0	35.0	2.0	35.0
96-97	27.741883591950902	34.0	29.0	35.0	2.0	35.0
98-99	27.39991497986933	34.0	29.0	35.0	2.0	35.0
100-101	26.252986156064747	33.0	23.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70539.0
3	12361.0
4	8740.0
5	6721.0
6	6190.0
7	6501.0
8	6730.0
9	7344.0
10	8124.0
11	8723.0
12	8883.0
13	9129.0
14	9707.0
15	10657.0
16	10890.0
17	11549.0
18	10949.0
19	10399.0
20	10599.0
21	11146.0
22	12220.0
23	13909.0
24	16067.0
25	19013.0
26	22079.0
27	26263.0
28	31131.0
29	36514.0
30	43472.0
31	51723.0
32	64032.0
33	82351.0
34	113329.0
35	161929.0
36	262691.0
37	470819.0
38	505229.0
39	93742.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89744698426061	17.849358773960667	11.803437778222003	25.449756463556717
2	16.7899924251766	20.071520425599378	37.83509785614882	25.303389293075202
3	19.298895361434003	23.508535363054587	29.765129990117966	27.42743928539344
4	13.660634506399363	15.071960077031038	36.00113265394648	35.266272762623124
5	14.141175724567479	37.28184591645852	34.35332330488024	14.22365505409376
6	34.01339257224969	34.76466174182659	16.736490248244507	14.485455437679207
7	28.80188337526974	31.97143072174486	21.099683067271513	18.12700283571389
8	27.002663307492515	34.13571468498719	20.277140670259115	18.58448133726117
9	27.32991118070427	14.539703747908463	19.34185174720784	38.788533324179426
10-11	25.819396786296107	24.858331240934927	28.17638731515833	21.145884657610633
12-13	26.008471004581036	22.583271251514446	27.81988826373017	23.58836948017435
14-15	23.333769176551282	24.347478867107	24.859814771034827	27.458937185306887
16-17	23.438149276332005	27.336778532943622	25.986019649963026	23.23905254076135
18-19	23.452810375362212	26.801684042264778	27.209293216298907	22.5362123660741
20-21	23.80294770757086	26.13261382918085	27.12870306588877	22.93573539735952
22-23	23.920018313728253	25.856254842572373	26.723339437909416	23.500387405789954
24-25	23.640331202530724	25.879324758177162	26.875582463645685	23.604761575646428
26-27	23.292802976867346	26.13401405023992	27.005656232827015	23.56752674006572
28-29	23.420047435436445	26.546922044010472	26.70470274512013	23.32832777543295
30-31	23.883171683051753	26.22064025614647	26.435437996160005	23.460750064641775
32-33	23.592704793956706	26.27650812239831	26.385763253263516	23.745023830381466
34-35	23.905998256374552	26.290962765543107	26.533406798554932	23.26963217952741
36-37	23.910148128540744	26.052281205051347	26.718519687139214	23.31905097926869
38-39	23.673902821195902	26.139844515497728	26.67731365767049	23.508939005635877
40-41	23.79872327354564	25.98080245593375	26.75708312100033	23.463391149520284
42-43	23.551836361334903	26.050979629406655	26.441393581986265	23.95579042727217
44-45	23.35013238623712	26.04388917916124	26.753638141345732	23.852340293255907
46-47	23.43699049912814	25.96348647360963	26.6468005191714	23.952722508090833
48-49	23.25078835627221	26.17265652802209	26.616742213341933	23.959812902363765
50-51	23.413638477152908	26.091084124953344	26.772317907922872	23.722959489970876
52-53	23.599459987075143	25.857782044415913	26.75864143049186	23.78411653801709
54-55	22.819050155579372	25.808383273139523	27.15792105479573	24.214645516485373
56-57	23.010567576464393	25.97221857811207	26.840749054619835	24.1764647908037
58-59	23.140662608893525	25.83099530689519	26.927758290549143	24.100583793662146
60-61	23.176022827002544	25.778636289465446	27.10977777189756	23.93556311163445
62-63	23.20038998440327	25.6742598387941	27.129557810425425	23.995792366377206
64-65	22.92790509549913	25.56789181387828	27.034756881738236	24.469446208884353
66-67	23.004017264913056	25.693100009634733	26.74696787003825	24.55591485541396
68-69	23.056921825360956	25.67115018728491	26.84591930544747	24.42600868190667
70-71	22.990537142695903	25.517970522866623	26.82420018949523	24.667292144942245
72-73	23.046301256909032	25.8033657206227	26.617413294001185	24.532919728467082
74-75	23.076020849326532	25.87615542369541	26.62370016901165	24.42412355796641
76-77	23.108602705709256	25.540747233599014	26.67235151028276	24.678298550408968
78-79	23.043131509950125	25.66522514982022	26.73911955387872	24.55252378635093
80-81	23.316727614088702	25.77089135246383	26.52290087530098	24.389480158146487
82-83	23.406365211431687	25.765485177190346	26.54389796044825	24.28425165092972
84-85	23.291288957822367	25.81254028275712	26.47419731610722	24.4219734433133
86-87	23.310337313914083	25.965807453162732	26.425573461442355	24.298281771480834
88-89	23.25893003832082	25.616552334919135	26.53678562505141	24.587732001708638
90-91	23.526481149606486	25.472963525426955	26.528610262881752	24.471945062084803
92-93	23.78909427879782	25.329103263212705	26.493107648242294	24.388694809747186
94-95	23.723313904546576	25.14568281978159	26.550695283566263	24.58030799210557
96-97	23.72814367401096	24.923545039944493	26.591968049700466	24.756343236344083
98-99	23.674168994465774	24.983117667322553	26.47374714566178	24.868966192549895
100-101	24.0617186226652	24.957229291798235	26.427500644565654	24.553551440970907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	190.0
1	154.5
2	201.0
3	300.5
4	270.0
5	275.5
6	434.0
7	671.0
8	965.0
9	1092.5
10	920.5
11	769.0
12	802.5
13	866.5
14	926.5
15	1015.5
16	1099.5
17	1200.5
18	1313.5
19	1437.0
20	1547.5
21	1668.5
22	1785.5
23	1940.0
24	2183.0
25	2476.0
26	3024.5
27	3957.0
28	5357.5
29	7425.5
30	10428.0
31	14795.5
32	21110.0
33	25495.0
34	28530.0
35	33487.0
36	39947.0
37	46697.0
38	52694.0
39	58337.5
40	63395.5
41	68280.5
42	72859.0
43	78041.5
44	82993.0
45	88574.0
46	93000.5
47	96001.5
48	99087.5
49	100650.0
50	101837.0
51	101364.0
52	99286.5
53	97695.5
54	94610.0
55	88115.0
56	79382.5
57	70244.5
58	62535.0
59	54022.0
60	44243.5
61	36544.0
62	30420.5
63	23438.0
64	16858.0
65	12316.0
66	9321.5
67	7252.0
68	5508.5
69	4217.0
70	3225.5
71	2418.0
72	1762.0
73	1322.5
74	1050.5
75	753.5
76	539.0
77	395.5
78	250.0
79	159.0
80	117.0
81	96.0
82	77.0
83	63.0
84	55.0
85	44.5
86	44.5
87	37.0
88	28.0
89	25.5
90	25.5
91	23.0
92	15.5
93	16.0
94	14.0
95	9.5
96	10.0
97	12.5
98	10.5
99	7.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.056548068278381956
2	0.07582281839972928
3	0.07107014028761624
4	0.07173023446985416
5	0.06737361286708388
6	0.010913557146333639
7	0.013729958990548772
8	0.01830661198739836
9	0.006556935543563356
10-11	0.01273981771719189
12-13	0.023565362305893806
14-15	0.04154192720217321
16-17	0.02829603727859891
18-19	0.011045575982781223
20-21	0.020594938485823156
22-23	0.03885754419440566
24-25	0.035293035610320884
26-27	0.021321042086284873
28-29	0.011529645049755698
30-31	0.011683667025611215
32-33	0.008735246344948497
34-35	0.0055447911307985424
36-37	8.141161580934368E-4
38-39	0.003806543117572015
40-41	0.001276182085659982
42-43	0.2229358084811541
44-45	0.19441973980847588
46-47	0.20060262198210438
48-49	0.2072695732227074
50-51	0.199458458732892
52-53	0.20592738171882363
54-55	0.20196681662539612
56-57	0.2052672875365857
58-59	0.21215427017126803
60-61	0.2162028478223273
62-63	0.20330900812927988
64-65	0.2027589296440816
66-67	0.20095467221263127
68-69	0.19662005374926897
70-71	0.1879948231013601
72-73	0.19970049326637923
74-75	0.19855633001716685
76-77	0.1929235263287366
78-79	0.2098439405334353
80-81	0.22053746628568965
82-83	0.2186451962966076
84-85	0.2129243800505456
86-87	0.2319790987778136
88-89	0.22432200626385373
90-91	0.23156103912906292
92-93	0.22634629508938336
94-95	0.23035086646162675
96-97	0.23684179258696628
98-99	0.23059290099511398
100-101	0.2364237329382156
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2272403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.465977853215094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33232832940314	46.5810850162706
2	11.878638377298758	14.127496933415976
3	3.906561540537188	6.969225061554186
4	1.8520338112325077	4.405320064086314
5	0.9870633654585848	2.9348344115040077
6	0.66401171206392	2.36916634583211
7	0.44694913357868254	1.8604787095232527
8	0.33042842376540027	1.5719399463764852
9	0.24222236427107907	1.2963590774367646
>10	1.296448521483424	13.29568928578187
>50	0.0454328604678636	1.8296190361175881
>100	0.016177353342687866	1.748094534337423
>500	8.891515287993619E-4	0.34011371038267824
>1k	8.150555680658032E-4	0.6705778673808124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2281	0.10037832197897997	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.400627881586145E-5
2	0.0	0.0	0.0	0.0	4.400627881586145E-5
3	0.0	0.0	0.0	0.0	4.400627881586145E-5
4	0.0	0.0	0.0	0.0	4.400627881586145E-5
5	0.0	0.0	0.0	0.0	4.400627881586145E-5
6	0.0	0.0	0.0	0.0	4.400627881586145E-5
7	0.0	0.0	0.0	0.0	4.400627881586145E-5
8	0.0	0.0	0.0	0.0	1.3201883644758434E-4
9	0.0	0.0	0.0	0.0	2.2003139407930723E-4
10-11	0.0	0.0	0.0	0.0	2.2003139407930723E-4
12-13	0.0	0.0	0.0	0.0	2.2003139407930723E-4
14-15	0.0	0.0	0.0	0.0	3.5205023052689154E-4
16-17	0.0	0.0	0.0	4.400627881586145E-5	4.4006278815861445E-4
18-19	0.0	0.0	0.0	4.400627881586145E-5	5.280753457903374E-4
20-21	0.0	4.400627881586145E-5	0.0	8.80125576317229E-5	5.500784851982681E-4
22-23	0.0	4.400627881586145E-5	0.0	3.080439517110301E-4	6.38091042829991E-4
24-25	0.0	4.400627881586145E-5	0.0	0.0010561506915806747	7.041004610537832E-4
26-27	0.0	4.400627881586145E-5	0.0	0.002024288825529627	7.481067398696446E-4
28-29	0.0	4.400627881586145E-5	0.0	0.009769393897121241	7.481067398696446E-4
30-31	0.0	4.400627881586145E-5	0.0	0.04055178592881632	7.701098792775754E-4
32-33	0.0	4.400627881586145E-5	0.0	0.11582452584334733	8.801255763172289E-4
34-35	0.0	4.400627881586145E-5	0.0	0.22757847089622746	0.0011001569703965363
36-37	0.0	4.400627881586145E-5	0.0	0.40708008218612635	0.0011001569703965363
38-39	0.0	4.400627881586145E-5	0.0	0.6630646060579923	0.0011441632492123976
40-41	0.0	4.400627881586145E-5	0.0	0.9347153651883051	0.00121017266743619
42-43	0.0	4.400627881586145E-5	0.0	1.2169276312344244	0.0012321758068441204
44-45	0.0	4.400627881586145E-5	0.0	1.5786152368219897	0.0012321758068441204
46-47	0.0	4.400627881586145E-5	0.0	1.9956187348810928	0.0013201883644758435
48-49	0.0	4.400627881586145E-5	0.0	2.4130622957283547	0.0014082009221075664
50-51	0.0	4.400627881586145E-5	0.0	2.8353685503847688	0.0014082009221075664
52-53	0.0	4.400627881586145E-5	0.0	3.2598751189819763	0.0014302040615154971
54-55	0.0	4.400627881586145E-5	0.0	3.752239369513242	0.0014522072009234277
56-57	0.0	4.400627881586145E-5	0.0	4.31369347778541	0.0014742103403313585
58-59	0.0	6.600941822379217E-5	0.0	4.88438890460891	0.0015182166191472198
60-61	0.0	8.80125576317229E-5	0.0	5.461971314067091	0.0015402197585551506
62-63	0.0	8.80125576317229E-5	0.0	6.052909629145887	0.0016282323161868736
64-65	0.0	8.80125576317229E-5	0.0	6.692100828946273	0.0016502354555948044
66-67	0.0	8.80125576317229E-5	0.0	7.419172567541937	0.001672238595002735
68-69	0.0	8.80125576317229E-5	0.0	8.201230151518018	0.001672238595002735
70-71	0.0	8.80125576317229E-5	0.0	8.985003980367917	0.0017162448738185965
72-73	0.0	8.80125576317229E-5	0.0	9.777293024168689	0.0017162448738185965
74-75	0.0	8.80125576317229E-5	0.0	10.590419040988767	0.0017162448738185965
76-77	0.0	8.80125576317229E-5	0.0	11.5085660422029	0.0017162448738185965
78-79	0.0	8.80125576317229E-5	0.0	12.51182118664691	0.0017162448738185965
80-81	0.0	8.80125576317229E-5	0.0	13.519586974669545	0.0017822542920423886
82-83	0.0	8.80125576317229E-5	0.0	14.539080435996608	0.0018922699890820422
84-85	0.0	8.80125576317229E-5	0.0	15.58260132555713	0.0018922699890820422
86-87	0.0	8.80125576317229E-5	0.0	16.696224217271322	0.0018922699890820422
88-89	0.0	8.80125576317229E-5	0.0	17.86991567956916	0.0018922699890820422
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1755	0.0	55.177456	1
GTATCAA	3340	0.0	42.778805	1
TCAACGC	4050	0.0	34.2252	4
CAACGCA	4175	0.0	33.426422	5
ATCAACG	4310	0.0	32.159863	3
TATCAAC	4490	0.0	31.188457	2
GTACATG	6090	0.0	31.17822	1
AACGCAG	4500	0.0	30.890484	6
TACATGG	6480	0.0	28.643095	2
ACATGGG	6750	0.0	26.512226	3
ACGCAGA	5470	0.0	24.548023	7
CATGGGG	5450	0.0	23.691393	4
GAGTACA	4715	0.0	23.658913	1
CGCAGAG	5725	0.0	23.372766	8
ATGGGGG	3570	0.0	21.938898	5
AGTACAT	5120	0.0	20.026358	2
ACCGTGC	525	0.0	19.883823	8
GCAGAGT	6370	0.0	19.814735	9
TCTATAC	530	0.0	18.808409	3
GTATATG	640	0.0	18.542515	1
>>END_MODULE