FastQCFastQC Report
Mon 15 Aug 2016
SRR2921661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272403
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.052.8902471
GTATCAA32000.037.428381
GTACATG57500.031.4098761
TACATGG61900.028.9814642
TCAACGC40650.028.3703634
CAACGCA41000.028.15115
TATCAAC42900.027.546172
ACATGGG64200.027.425723
ATCAACG42700.027.230613
AACGCAG43250.026.6830676
CATGGGG52050.025.3479714
ATGGGGG34850.023.9873815
GAGTACA42550.021.7813851
ACGCAGA54050.021.1756577
CGCAGAG56550.020.1555298
AGTACAT47700.018.2075652
TAGACCG1901.8673671E-417.4991685
AGAGTAC52000.017.44436510-11
CCTACAC7100.017.3793743
GCAGAGT61550.017.2068739
Produced by FastQC (version 0.11.5)