##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2272403
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244191281212004	33.0	31.0	34.0	28.0	34.0
2	31.373932792730866	33.0	31.0	34.0	28.0	34.0
3	31.445101067020243	34.0	31.0	34.0	28.0	34.0
4	34.9323632295856	37.0	35.0	37.0	32.0	37.0
5	34.66024644396263	37.0	35.0	37.0	32.0	37.0
6	34.62996748376058	37.0	35.0	37.0	32.0	37.0
7	34.505425314083816	37.0	35.0	37.0	31.0	37.0
8	34.45091825701691	37.0	35.0	37.0	31.0	37.0
9	36.018829406579734	39.0	37.0	39.0	31.0	39.0
10-11	35.90677313839139	39.0	37.0	39.0	30.5	39.0
12-13	35.823914156071794	39.0	37.0	39.0	30.0	39.0
14-15	37.03328678935911	40.0	37.0	41.0	31.0	41.0
16-17	36.99142845701225	40.0	37.0	41.0	31.0	41.0
18-19	36.953756882031925	40.0	37.0	41.0	30.5	41.0
20-21	36.88819060703581	40.0	37.0	41.0	30.0	41.0
22-23	36.79485416979294	40.0	37.0	41.0	30.0	41.0
24-25	36.7007812874741	40.0	37.0	41.0	30.0	41.0
26-27	36.56721497023196	40.0	37.0	41.0	30.0	41.0
28-29	36.437262008543385	40.0	37.0	41.0	30.0	41.0
30-31	36.26223649590324	40.0	36.0	41.0	29.0	41.0
32-33	36.191415871216506	40.0	36.0	41.0	29.0	41.0
34-35	36.308929578072195	40.0	36.5	41.0	29.5	41.0
36-37	36.292887749224064	40.0	36.0	41.0	29.0	41.0
38-39	36.17544093191216	40.0	36.0	41.0	28.5	41.0
40-41	36.08148356607521	40.0	36.0	41.0	28.0	41.0
42-43	35.94177221205922	40.0	36.0	41.0	27.5	41.0
44-45	35.795926162744905	40.0	35.5	41.0	27.0	41.0
46-47	35.63301161809767	40.0	35.0	41.0	26.5	41.0
48-49	35.479273702771906	40.0	35.0	41.0	26.0	41.0
50-51	35.30825210140983	39.0	35.0	41.0	26.0	41.0
52-53	35.121442147365585	39.0	35.0	41.0	25.5	41.0
54-55	34.904358513872765	39.0	35.0	41.0	24.0	41.0
56-57	34.67576789856377	39.0	35.0	41.0	24.0	41.0
58-59	34.42860685362588	38.0	34.0	40.0	23.0	41.0
60-61	34.14383562246661	38.0	34.0	40.0	23.0	41.0
62-63	33.829737726978884	37.0	34.0	40.0	22.0	41.0
64-65	33.48816583150084	37.0	34.0	40.0	21.5	41.0
66-67	33.131280191057655	36.0	33.0	39.0	20.0	41.0
68-69	32.76489535526929	36.0	33.0	39.0	19.5	40.5
70-71	32.368061254979864	35.0	33.0	38.5	18.0	40.0
72-73	31.99717523696281	35.0	33.0	37.5	15.5	39.5
74-75	31.586176175616735	35.0	32.0	37.0	11.5	39.0
76-77	28.880058026679244	32.0	28.0	34.5	7.0	36.5
78-79	30.44524606770894	34.5	31.0	35.5	7.0	37.0
80-81	30.62499345406603	35.0	31.5	35.5	4.5	37.0
82-83	30.443653480478595	35.0	32.0	35.0	2.0	37.0
84-85	30.23936049195499	35.0	31.0	35.0	2.0	36.0
86-87	30.02517313170243	35.0	31.0	35.0	2.0	36.0
88-89	29.748461210445505	34.5	31.0	35.0	2.0	36.0
90-91	29.53897416083327	34.0	31.0	35.0	2.0	35.0
92-93	29.365092371379546	34.0	31.0	35.0	2.0	35.0
94-95	29.246982159414507	34.0	31.0	35.0	2.0	35.0
96-97	29.03204801261044	34.0	31.0	35.0	2.0	35.0
98-99	28.80998924926609	34.0	30.5	35.0	2.0	35.0
100-101	27.633706477240175	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47339.0
3	15985.0
4	8661.0
5	6185.0
6	5438.0
7	5526.0
8	5888.0
9	6111.0
10	6742.0
11	7329.0
12	7931.0
13	8412.0
14	9270.0
15	10006.0
16	10950.0
17	11639.0
18	10980.0
19	9898.0
20	9732.0
21	9578.0
22	9916.0
23	11220.0
24	12999.0
25	15415.0
26	18262.0
27	21532.0
28	26060.0
29	31166.0
30	38149.0
31	46564.0
32	59033.0
33	77266.0
34	110320.0
35	163507.0
36	278567.0
37	527336.0
38	544961.0
39	66529.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76257234522606	19.378713677528605	12.272738692415272	26.58597528483006
2	15.879392606400817	21.360034641818906	36.308164326847674	26.452408424932607
3	18.043392057009893	24.552288630193114	29.47023700282594	27.934082309971053
4	13.331572971402919	16.141902775210585	34.89129765079701	35.63522660258949
5	13.875436760126805	37.638576978504666	34.428670655793844	14.057315605574683
6	34.183977253574156	35.221402270857965	16.40666497385754	14.18795550171034
7	28.674359257578867	32.11322991564437	21.08499240671659	18.127418420060174
8	26.985442282904927	34.56605188428285	20.027697551886703	18.42080828092552
9	27.027072222664728	14.56317387364829	19.229819710676317	39.17993419301066
10-11	25.765044428183607	25.094923361273207	28.141188373561288	20.998843836981894
12-13	25.975121848287415	22.6881060097153	27.82796832681635	23.50880381518094
14-15	23.18538196827473	24.507132563874453	24.831805102298805	27.475680365552012
16-17	23.423684091246137	27.58394967794005	25.855185017798338	23.137181213015474
18-19	23.45814238868808	26.942288528094853	27.125115710969784	22.47445337224728
20-21	23.712659490106926	26.204058443987364	27.10830145071418	22.97498061519153
22-23	23.841479702323927	25.963462466824765	26.701601784542618	23.493456046308687
24-25	23.51652694260308	26.018006108531722	26.826866185925823	23.638600762939372
26-27	23.230800746985096	26.29793280874801	26.960313281341282	23.510953162925617
28-29	23.364521558426926	26.737787643548096	26.610954599095137	23.28673619892984
30-31	23.842218830703686	26.342408640816544	26.378302008246013	23.437070520233757
32-33	23.49429313149047	26.40374309486185	26.32115540898729	23.78080836466039
34-35	23.827518991765135	26.41255998123379	26.453710089346348	23.306210937654726
36-37	23.79677926513027	26.23986850371264	26.680147823302963	23.28320440785413
38-39	23.630782838867674	26.310435761270284	26.60849035327148	23.450291046590557
40-41	23.709172251631703	26.129758955710926	26.636663569979767	23.524405222677604
42-43	23.528741254878817	26.141038480183592	26.35723906679685	23.97298119814074
44-45	23.277545432100613	26.17818915896537	26.644205688051027	23.90005972088299
46-47	23.343099406004207	26.05646865706782	26.607820852893898	23.992611084034067
48-49	23.190957118135934	26.274317884548694	26.561308971585195	23.973416025730174
50-51	23.343713989417495	26.200202390888137	26.65244634870159	23.803637270992773
52-53	23.552602279398368	25.94960996394048	26.619620006389848	23.878167750271302
54-55	22.76244316909085	25.938944977162016	26.9672813272479	24.331330526499233
56-57	22.93394008276056	26.004750865619158	26.680962539897703	24.38034651172258
58-59	22.974413212024462	25.917524316680325	26.753068757464344	24.35499371383087
60-61	22.962994095673327	25.993164430392675	26.818234229603316	24.225607244330686
62-63	22.951054543837962	25.960443354513046	26.770820013939833	24.317682087709155
64-65	22.648544655298036	25.73531062688893	26.727383989557353	24.888760728255676
66-67	22.72829450989691	25.927454874786793	26.418452949852412	24.925797665463886
68-69	22.728811738464643	25.851871713790832	26.442585836598077	24.976730711146452
70-71	22.54617578659461	25.67694321863387	26.42957452177241	25.347306472999115
72-73	22.53606131667442	25.91191648944342	26.31277631748662	25.23924587639554
74-75	22.415015372317328	25.91559872584409	26.297634210377463	25.37175169146112
76-77	22.316419377082966	25.621711880639435	26.432129864465036	25.629738877812564
78-79	22.335448153147897	25.591250327573185	26.510194958345906	25.56310656093302
80-81	22.399780876112434	25.753155795718936	26.239469029705248	25.607594298463383
82-83	22.313282170437194	25.704590288557768	26.323668814997685	25.65845872600735
84-85	22.14974728189621	25.716243767354484	26.289375272569888	25.84463367817942
86-87	22.063957975216773	25.87636218236295	26.207339496809674	25.852340345610596
88-89	21.881741548239035	25.504766528378497	26.32880218907229	26.28468973431018
90-91	21.898585176305676	25.296660140423104	26.410123210064423	26.394631473206797
92-93	21.986711637784808	25.11853130157573	26.461985117791613	26.43277194284785
94-95	21.747494407872658	25.012724634077372	26.602506197276387	26.63727476077358
96-97	21.624590127858102	24.77289232191193	26.6892674016857	26.913250148544265
98-99	21.410299232127684	24.81714130056421	26.61674990527565	27.155809562032456
100-101	21.350032586735505	24.80684493070533	26.590820177988267	27.252302304570904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	171.0
1	145.5
2	170.0
3	236.5
4	240.5
5	257.5
6	366.0
7	623.5
8	877.0
9	934.0
10	788.0
11	628.5
12	592.5
13	605.0
14	664.0
15	720.0
16	738.5
17	798.5
18	873.0
19	936.5
20	1048.5
21	1168.0
22	1319.5
23	1546.5
24	1794.0
25	2134.5
26	2800.0
27	3869.0
28	5452.5
29	7690.0
30	10777.0
31	14921.5
32	21212.0
33	25957.5
34	28807.0
35	33575.0
36	39821.5
37	45963.5
38	51660.0
39	57684.5
40	63059.5
41	67664.5
42	72340.0
43	77814.5
44	82997.0
45	88610.5
46	93161.5
47	96474.0
48	100006.0
49	102108.5
50	102952.0
51	102546.0
52	101082.5
53	99315.0
54	96127.5
55	89328.5
56	80418.0
57	71029.5
58	62520.5
59	53889.0
60	44490.0
61	36646.5
62	30203.0
63	23232.5
64	16445.5
65	11893.5
66	8895.0
67	6824.5
68	5257.0
69	3999.5
70	3013.0
71	2190.5
72	1542.5
73	1164.5
74	921.0
75	650.5
76	432.5
77	264.0
78	142.5
79	82.0
80	50.0
81	40.5
82	32.0
83	19.5
84	11.0
85	6.0
86	5.0
87	5.5
88	5.0
89	2.5
90	2.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15815856606420603
2	2.2003139407930723E-4
3	0.010385481800543302
4	0.006292897870668186
5	0.02468752241569827
6	0.0020242888255296264
7	0.0
8	0.0
9	0.0
10-11	0.04827488786100001
12-13	3.300470911189608E-4
14-15	0.004994712645600274
16-17	0.0
18-19	0.0016942417344106655
20-21	2.2003139407930723E-4
22-23	0.0
24-25	3.300470911189608E-4
26-27	0.0030144300988865095
28-29	0.00547878171257475
30-31	0.0174044832716732
32-33	0.011991710977322243
34-35	0.010297469242911578
36-37	0.004202599626914768
38-39	2.2003139407930725E-5
40-41	0.0036085148629006383
42-43	0.00913130285429125
44-45	0.007305042283433
46-47	0.0035425054446768466
48-49	0.009109299714883319
50-51	0.0029704238200706475
52-53	0.002156307661977211
54-55	0.004048577651059253
56-57	0.008163164720342298
58-59	0.015468207003775297
60-61	0.007525073677512308
62-63	0.021849117432075207
64-65	0.04171795231743665
66-67	0.062334893942667745
68-69	0.05144333993574203
70-71	0.06908985774090247
72-73	0.03458893514926709
74-75	0.027481921120505473
76-77	0.2224297362747717
78-79	0.08440404276882225
80-81	0.06783567879465043
82-83	0.028142015302743392
84-85	0.05265351260317822
86-87	0.0683197478616249
88-89	0.29057345902113313
90-91	0.2939179362111386
92-93	0.051377330517518235
94-95	0.010363478661135371
96-97	0.01553421642199909
98-99	8.801255763172289E-4
100-101	2.640376728951687E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2272403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.20254473155293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05248586506795	46.209057858355884
2	11.959766182624236	14.160971848114507
3	4.041742725581831	7.178443635140609
4	1.8144381825144533	4.296774306517981
5	1.0749252205569564	3.181915422654881
6	0.6519009421674943	2.315651681352755
7	0.45315685386979976	1.8779627238145629
8	0.33143333594811053	1.569735751759666
9	0.25669692892455287	1.3677400275397293
>10	1.2990496047515985	13.291847057385041
>50	0.04690135732921941	1.8612204313475302
>100	0.01571767826723714	1.6919930603704132
>500	0.0010413213979422386	0.4066183763631414
>1k	7.438009985294367E-4	0.5900678192832903
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.80125576317229E-5
2	0.0	0.0	0.0	0.0	8.80125576317229E-5
3	0.0	0.0	0.0	0.0	8.80125576317229E-5
4	0.0	0.0	0.0	0.0	8.80125576317229E-5
5	0.0	0.0	0.0	0.0	8.80125576317229E-5
6	0.0	0.0	0.0	0.0	1.3201883644758434E-4
7	0.0	0.0	0.0	0.0	1.3201883644758434E-4
8	0.0	0.0	0.0	0.0	1.3201883644758434E-4
9	0.0	0.0	0.0	0.0	1.760251152634458E-4
10-11	0.0	0.0	0.0	0.0	1.760251152634458E-4
12-13	0.0	0.0	0.0	0.0	2.2003139407930723E-4
14-15	0.0	0.0	0.0	4.400627881586145E-5	3.080439517110301E-4
16-17	0.0	0.0	0.0	4.400627881586145E-5	3.96056509342753E-4
18-19	0.0	0.0	0.0	4.400627881586145E-5	4.4006278815861445E-4
20-21	0.0	0.0	0.0	8.80125576317229E-5	4.4006278815861445E-4
22-23	0.0	0.0	0.0	3.080439517110301E-4	5.500784851982681E-4
24-25	0.0	0.0	0.0	0.0010561506915806747	6.600941822379218E-4
26-27	0.0	0.0	0.0	0.002068295104345488	6.600941822379218E-4
28-29	0.0	0.0	0.0	0.009791397036529172	6.600941822379218E-4
30-31	0.0	0.0	0.0	0.04059579220763219	6.600941822379218E-4
32-33	0.0	0.0	0.0	0.11613256979505836	6.600941822379218E-4
34-35	0.0	0.0	0.0	0.22821656193905746	7.041004610537832E-4
36-37	0.0	0.0	0.0	0.40776217950777216	7.041004610537832E-4
38-39	0.0	0.0	0.0	0.6642087693072047	7.261036004617138E-4
40-41	0.0	0.0	0.0	0.9355074782069905	8.361192975013675E-4
42-43	0.0	0.0	0.0	1.2173676940225833	8.361192975013675E-4
44-45	0.0	0.0	0.0	1.5780431551973835	8.581224369092982E-4
46-47	0.0	0.0	0.0	1.9920542262970082	9.241318551330903E-4
48-49	0.0	0.0	0.0	2.4080235768039384	0.0011881695280282591
50-51	0.0	0.0	0.0	2.828965636817061	0.0011881695280282591
52-53	0.0	0.0	0.0	3.251821969958674	0.0012981852250679128
54-55	0.0	0.0	0.0	3.7406657181846708	0.0014082009221075664
56-57	0.0	0.0	0.0	4.300249559607165	0.0014302040615154971
58-59	0.0	0.0	0.0	4.867314468428356	0.0014522072009234277
60-61	4.400627881586145E-5	0.0	0.0	5.442608551388112	0.0014962134797392892
62-63	4.400627881586145E-5	0.0	0.0	6.0326667408905905	0.001584226037371012
64-65	4.400627881586145E-5	0.0	0.0	6.670911805696436	0.0016062291767789429
66-67	4.400627881586145E-5	0.0	0.0	7.396025264884794	0.0016282323161868736
68-69	4.400627881586145E-5	0.0	0.0	8.176586635381137	0.0016282323161868736
70-71	4.400627881586145E-5	0.0	0.0	8.960008414000509	0.0016282323161868736
72-73	4.400627881586145E-5	0.0	0.0	9.752231448383057	0.0018482637102661807
74-75	4.400627881586145E-5	0.0	0.0	10.567601785422744	0.0018922699890820422
76-77	4.400627881586145E-5	0.0	0.0	11.48933529836037	0.0018922699890820422
78-79	4.400627881586145E-5	0.0	0.0	12.498795328117417	0.0018922699890820422
80-81	4.400627881586145E-5	0.0	0.0	13.510939740882229	0.0019582794073058343
82-83	4.400627881586145E-5	0.0	0.0	14.533777679399297	0.002068295104345488
84-85	4.400627881586145E-5	0.0	0.0	15.578970807554821	0.002068295104345488
86-87	4.400627881586145E-5	0.0	0.0	16.697786440169285	0.0020902982437534185
88-89	4.400627881586145E-5	0.0	0.0	17.878232866265357	0.0021123013831613495
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1950	0.0	52.890247	1
GTATCAA	3200	0.0	37.42838	1
GTACATG	5750	0.0	31.409876	1
TACATGG	6190	0.0	28.981464	2
TCAACGC	4065	0.0	28.370363	4
CAACGCA	4100	0.0	28.1511	5
TATCAAC	4290	0.0	27.54617	2
ACATGGG	6420	0.0	27.42572	3
ATCAACG	4270	0.0	27.23061	3
AACGCAG	4325	0.0	26.683067	6
CATGGGG	5205	0.0	25.347971	4
ATGGGGG	3485	0.0	23.987381	5
GAGTACA	4255	0.0	21.781385	1
ACGCAGA	5405	0.0	21.175657	7
CGCAGAG	5655	0.0	20.155529	8
AGTACAT	4770	0.0	18.207565	2
TAGACCG	190	1.8673671E-4	17.499168	5
AGAGTAC	5200	0.0	17.444365	10-11
CCTACAC	710	0.0	17.379374	3
GCAGAGT	6155	0.0	17.206873	9
>>END_MODULE