FastQCFastQC Report
Mon 15 Aug 2016
SRR2921660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764286
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.045.5659331
GTATCAA36450.040.107191
TCAACGC43350.032.0798154
CAACGCA43750.031.5683985
ATCAACG44750.031.2894573
TATCAAC47650.029.6856122
AACGCAG47450.029.3893056
GTACATG67150.027.8496171
TACATGG70900.025.9763492
ACATGGG72950.024.3341053
ACGCAGA55650.024.2965357
CGCAGAG58300.023.0310618
CATGGGG61000.022.2530484
GAGTACA51150.022.085151
GCAGAGT65900.019.6553699
AGAGTAC55200.019.03910310-11
CGTATAC2001.2941129E-518.9858063
AGTACAT57500.017.6660142
ATAGGAC8150.016.8892123
GTATATG9650.016.7232481
Produced by FastQC (version 0.11.5)