##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921660_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2764286
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.63932964968169	31.0	31.0	34.0	27.0	34.0
2	30.69472695661737	33.0	31.0	34.0	27.0	34.0
3	30.750814857796914	33.0	31.0	34.0	27.0	34.0
4	34.22613615233735	37.0	35.0	37.0	30.0	37.0
5	34.231978890751535	37.0	35.0	37.0	30.0	37.0
6	34.28354591384539	37.0	35.0	37.0	30.0	37.0
7	34.19756928190498	37.0	35.0	37.0	30.0	37.0
8	34.17255088655805	37.0	35.0	37.0	30.0	37.0
9	35.75902891379546	39.0	37.0	39.0	30.0	39.0
10-11	35.662342282962044	39.0	37.0	39.0	30.0	39.0
12-13	35.55969154421793	39.0	37.0	39.0	30.0	39.0
14-15	36.731847030300045	40.0	37.0	41.0	30.0	41.0
16-17	36.67085117097145	40.0	37.0	41.0	30.0	41.0
18-19	36.64343758207363	40.0	37.0	41.0	30.0	41.0
20-21	36.57144684016053	40.0	37.0	41.0	29.5	41.0
22-23	36.47286930512979	40.0	37.0	41.0	29.0	41.0
24-25	36.36479293387153	40.0	37.0	41.0	29.0	41.0
26-27	36.22231183748715	40.0	36.0	41.0	28.0	41.0
28-29	36.087544667954035	40.0	36.0	41.0	27.0	41.0
30-31	35.93700905043834	40.0	36.0	41.0	27.0	41.0
32-33	35.75635299675938	40.0	36.0	41.0	27.0	41.0
34-35	35.58394590863608	39.5	35.0	41.0	26.0	41.0
36-37	35.54257410412671	39.5	35.0	41.0	25.5	41.0
38-39	35.709403621767066	40.0	35.5	41.0	26.5	41.0
40-41	35.76373175568664	40.0	36.0	41.0	26.5	41.0
42-43	35.590437639231254	40.0	35.0	41.0	26.0	41.0
44-45	35.46492421551171	40.0	35.0	41.0	25.0	41.0
46-47	35.30010136433061	40.0	35.0	41.0	24.0	41.0
48-49	35.127486085014354	40.0	35.0	41.0	24.0	41.0
50-51	33.4257332634901	37.0	32.5	39.5	21.0	40.0
52-53	33.538906610965725	37.5	33.5	39.5	21.5	40.0
54-55	34.45136031510488	39.0	34.0	40.5	22.0	41.0
56-57	34.46429729051191	39.0	34.0	41.0	22.0	41.0
58-59	34.27411291740435	38.5	34.0	41.0	22.0	41.0
60-61	34.020205036671314	38.0	34.0	40.5	21.0	41.0
62-63	33.730459511063614	37.0	34.0	40.0	20.0	41.0
64-65	33.43560398598409	37.0	34.0	40.0	19.5	41.0
66-67	33.09685719929125	36.0	33.0	39.5	18.0	41.0
68-69	32.73541069918235	36.0	33.0	39.0	15.5	41.0
70-71	32.32982875143889	35.0	33.0	39.0	12.0	41.0
72-73	31.969670830008184	35.0	32.5	37.5	8.0	40.0
74-75	31.577344023013538	35.0	32.0	37.0	6.5	39.0
76-77	31.202623389909725	35.0	32.0	37.0	2.0	39.0
78-79	30.860185414967916	35.0	32.0	36.0	2.0	39.0
80-81	30.542879427092565	35.0	31.5	36.0	2.0	37.0
82-83	30.22803121674096	35.0	31.0	35.0	2.0	37.0
84-85	29.96074845366941	35.0	31.0	35.0	2.0	36.5
86-87	29.7194635432079	35.0	31.0	35.0	2.0	36.0
88-89	29.480469459383002	35.0	31.0	35.0	2.0	36.0
90-91	29.243393954171168	35.0	31.0	35.0	2.0	35.5
92-93	29.042425602850066	34.5	30.5	35.0	2.0	35.0
94-95	28.806794412734426	34.0	30.0	35.0	2.0	35.0
96-97	28.56105156991715	34.0	30.0	35.0	2.0	35.0
98-99	28.278942736026593	34.0	30.0	35.0	2.0	35.0
100-101	27.19489408838304	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78590.0
3	14354.0
4	9872.0
5	7771.0
6	7182.0
7	7469.0
8	7743.0
9	8432.0
10	9448.0
11	10293.0
12	10411.0
13	10465.0
14	11625.0
15	12378.0
16	12452.0
17	13163.0
18	12341.0
19	11229.0
20	11458.0
21	12025.0
22	13182.0
23	14982.0
24	17928.0
25	20638.0
26	24708.0
27	29213.0
28	34337.0
29	40749.0
30	48781.0
31	59597.0
32	73951.0
33	96149.0
34	132361.0
35	193755.0
36	327730.0
37	594802.0
38	637930.0
39	124776.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05209520238252	18.16368103524411	12.521409532689443	25.262814229683926
2	16.20215971837064	19.664059094667607	38.71104028220861	25.422740904753148
3	18.923840388313522	23.3560462570376	29.90275509140196	27.81735826324691
4	13.279098551900926	15.48160218020346	36.40328695791314	34.83601230998247
5	14.029711023964953	37.17671785749322	34.72483618464898	14.068734933892845
6	33.95002255782999	35.175743042618926	16.766817160738015	14.107417238813072
7	28.738603990150168	31.771268632145954	21.219824407921255	18.27030296978262
8	26.581580484161943	34.582308471061076	20.161799792024418	18.674311252752567
9	26.670653500708006	14.888256494523711	19.501226078091296	38.939863926676985
10-11	25.636530000206225	24.923107704208245	28.33457130442113	21.105790991164397
12-13	25.991209830602983	22.615879584123274	27.858284073394824	23.534626511878923
14-15	23.092203858713372	24.318196376670244	24.966243224668666	27.623356539947718
16-17	23.438870817133438	27.371083369926154	25.943194559258743	23.246851253681672
18-19	23.563763990979783	26.563442357181387	27.15661974093465	22.716173910904182
20-21	23.932575671092646	25.90562251139361	27.05937521540257	23.102426602111176
22-23	23.873794241164255	25.809974814073065	26.698711064636036	23.617519880126643
24-25	23.6116878612152	26.00098033947104	26.773418361226327	23.613913438087437
26-27	23.443504495538477	26.131817868501273	26.725897981480884	23.698779654479374
28-29	23.582861320095798	26.38147425151282	26.59799342139182	23.437671006999565
30-31	23.78807687291208	26.041072524629023	26.48836481076367	23.682485791695225
32-33	23.514664635828435	26.125093725777145	26.388873563135224	23.971368075259196
34-35	23.998562300695138	26.12105001250842	26.396777874914733	23.483609811881713
36-37	24.061798694806992	25.89548652255002	26.45368181891356	23.589032963729426
38-39	23.762536706144274	26.02461559165945	26.484100365279723	23.728747336916552
40-41	23.927563859418928	25.917701891160206	26.553261809554147	23.60147243986672
42-43	23.81736567382397	25.96658811594471	26.301548118163637	23.91449809206768
44-45	23.70469577288919	25.99099103180867	26.52418928800879	23.780123907293348
46-47	23.96370542629758	26.011908613273466	26.223579837242067	23.800806123186888
48-49	23.655126400255785	26.182007740637005	26.18563282235994	23.977233036747272
50-51	23.768027266858464	26.15083258814408	26.32893493455686	23.7522052104406
52-53	23.998752263606608	26.001456175796704	26.28324812390943	23.716543436687257
54-55	23.44084735256696	25.936542804426704	26.604862417579234	24.017747425427103
56-57	23.536752380897145	26.092501188998575	26.41742767408679	23.95331875601749
58-59	23.62833817041425	25.87114132451366	26.506043314940282	23.99447719013181
60-61	23.462487913624148	25.933184230265987	26.729407661538694	23.874920194571164
62-63	23.57399011621226	25.94077412249937	26.647766179147187	23.83746958214118
64-65	23.32328083304953	25.881838202896418	26.587809003823743	24.207071960230305
66-67	23.33122911527327	25.95861994468861	26.538648287291554	24.171502652746565
68-69	23.598023117993105	25.799053242427206	26.572009032618187	24.030914606961495
70-71	23.456458336127124	25.722474740793444	26.498563114762714	24.322503808316718
72-73	23.42192125029814	25.8709509976562	26.458006949483938	24.249120802561723
74-75	23.473105702113838	26.0164766155373	26.40613610389083	24.10428157845803
76-77	23.563696451457634	25.78534116807878	26.425158173709818	24.22580420675377
78-79	23.502237657537798	25.81305182411232	26.482554609175757	24.202155909174124
80-81	23.600119935594442	25.907955594825076	26.368699710349013	24.12322475923147
82-83	23.540337518567437	25.976428736391888	26.344387567349447	24.13884617769123
84-85	23.5038295532058	25.998091975620934	26.417074262317282	24.08100420885598
86-87	23.611127982532366	26.06329387717287	26.238071728902874	24.08750641139189
88-89	23.410714651532075	25.911450236647205	26.302801459821463	24.37503365199926
90-91	23.425573089981082	25.861670098734034	26.37464904255655	24.33810776872834
92-93	23.604629048981092	25.725965275062123	26.509759216282202	24.159646459674576
94-95	23.553163194913434	25.54833878896714	26.613878008341636	24.28462000777779
96-97	23.602058331479412	25.36078911530584	26.62207105598729	24.41508149722746
98-99	23.502272148347313	25.463055040108202	26.47576329771081	24.55890951383368
100-101	23.690510186597912	25.359855747656308	26.550209552094294	24.399424513651482
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	42.5
2	67.5
3	105.0
4	100.0
5	109.0
6	188.5
7	336.5
8	510.0
9	601.0
10	548.5
11	515.0
12	594.5
13	669.0
14	720.5
15	814.5
16	925.5
17	993.0
18	1128.0
19	1306.0
20	1422.5
21	1587.5
22	1826.5
23	2140.5
24	2540.0
25	3280.0
26	4647.5
27	6860.5
28	9904.5
29	13925.5
30	18973.0
31	25439.0
32	33864.5
33	38566.0
34	40606.5
35	44458.0
36	50165.0
37	56467.5
38	61916.5
39	67381.0
40	72759.5
41	78445.5
42	84430.0
43	90742.0
44	96408.0
45	103290.5
46	108275.0
47	111277.0
48	115079.0
49	116386.0
50	115961.5
51	115460.5
52	114428.0
53	113432.5
54	111868.0
55	106814.0
56	98706.5
57	89322.0
58	80948.5
59	71241.5
60	58738.0
61	48498.5
62	40781.5
63	31656.0
64	22689.0
65	16812.0
66	12778.5
67	10042.5
68	7810.0
69	5856.0
70	4539.0
71	3342.5
72	2311.0
73	1714.5
74	1347.5
75	1027.0
76	758.0
77	528.0
78	333.0
79	222.5
80	167.5
81	140.5
82	112.0
83	91.0
84	63.5
85	52.0
86	50.5
87	43.5
88	36.5
89	30.0
90	27.5
91	23.0
92	22.5
93	21.0
94	16.0
95	14.5
96	9.0
97	8.5
98	9.0
99	8.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0541912088691257
2	0.07502841601773477
3	0.07170025098705417
4	0.0714470210390676
5	0.06790180176725563
6	0.010093022212607524
7	0.013746768604985157
8	0.018087853427612047
9	0.006801032888782131
10-11	0.012842375933604554
12-13	0.022808783172218794
14-15	0.041131778694389796
16-17	0.02769250359767405
18-19	0.009875967971476178
20-21	0.02045736222662923
22-23	0.03749612015543978
24-25	0.03463823931387707
26-27	0.021759687673417294
28-29	0.011847543995085892
30-31	0.011630489753954547
32-33	0.008627906084970948
34-35	0.005335916761145555
36-37	7.41601990532094E-4
38-39	0.003726097806088082
40-41	0.001103359059084335
42-43	0.22412659182154093
44-45	0.19420928225227058
46-47	0.19992504393539595
48-49	0.20719636101329603
50-51	0.19820669785977282
52-53	0.20585785985965271
54-55	0.20171574142472956
56-57	0.20482685221427885
58-59	0.21370798824723636
60-61	0.21759687673417297
62-63	0.203560702474346
64-65	0.202674397656393
66-67	0.20046767953822436
68-69	0.19520411419078923
70-71	0.18786044569917873
72-73	0.19968990184083701
74-75	0.19777258937751013
76-77	0.19151419209155637
78-79	0.21057878960425946
80-81	0.22208266438422075
82-83	0.22056328469630132
84-85	0.21472090803918262
86-87	0.23512400670552902
88-89	0.22709299978366926
90-91	0.23416535047386558
92-93	0.22987852921152155
94-95	0.23273641005308426
96-97	0.23932038870073505
98-99	0.23273641005308426
100-101	0.2394289158213007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2764286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.97333669902155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6246849465646	48.549827248003965
2	11.262182157888741	13.733856493573269
3	3.7132639563738747	6.792302803929753
4	1.67602615633608	4.087716285865869
5	0.966549518471139	2.946687461300896
6	0.5813530586535417	2.1268221471773057
7	0.404513808223398	1.726518963974608
8	0.29293323750778094	1.4288893536717102
9	0.23225049479723142	1.2744978856007352
>10	1.1851857417309106	12.467065231065003
>50	0.04456455887924891	1.8342570129104416
>100	0.01423079503874089	1.600785050826492
>500	0.001190299755192587	0.5013225035362577
>1k	0.0010712697795038635	0.929451558563662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.61757068552241E-5
7	0.0	0.0	0.0	3.61757068552241E-5	3.61757068552241E-5
8	0.0	0.0	0.0	3.61757068552241E-5	7.23514137104482E-5
9	0.0	0.0	0.0	3.61757068552241E-5	7.23514137104482E-5
10-11	0.0	0.0	0.0	3.61757068552241E-5	7.23514137104482E-5
12-13	0.0	0.0	0.0	7.23514137104482E-5	9.043926713806024E-5
14-15	0.0	0.0	0.0	7.23514137104482E-5	1.2661497399328435E-4
16-17	0.0	0.0	0.0	9.043926713806024E-5	1.8087853427612048E-4
18-19	0.0	0.0	0.0	1.0852712056567229E-4	1.8087853427612048E-4
20-21	0.0	0.0	0.0	1.0852712056567229E-4	2.1705424113134457E-4
22-23	0.0	0.0	0.0	1.2661497399328435E-4	2.1705424113134457E-4
24-25	0.0	0.0	0.0	2.532299479865687E-4	2.7131780141418076E-4
26-27	0.0	0.0	0.0	4.341084822626892E-4	2.894056548417928E-4
28-29	0.0	0.0	0.0	0.003129198642976884	2.894056548417928E-4
30-31	0.0	0.0	0.0	0.015410851120325465	3.4366921512462895E-4
32-33	3.61757068552241E-5	0.0	0.0	0.043935395975669665	4.702841891179133E-4
34-35	3.61757068552241E-5	0.0	0.0	0.0837648492232714	5.064598959731374E-4
36-37	7.23514137104482E-5	0.0	0.0	0.14519119946344192	5.064598959731374E-4
38-39	7.23514137104482E-5	0.0	0.0	0.24456586619474252	5.064598959731374E-4
40-41	7.23514137104482E-5	0.0	0.0	0.35602321901568795	5.064598959731374E-4
42-43	7.23514137104482E-5	3.61757068552241E-5	0.0	0.4727984007443513	5.064598959731374E-4
44-45	7.23514137104482E-5	3.61757068552241E-5	0.0	0.6215167316261776	5.064598959731374E-4
46-47	7.23514137104482E-5	3.61757068552241E-5	0.0	0.796570977098607	5.064598959731374E-4
48-49	7.23514137104482E-5	3.61757068552241E-5	0.0	0.9742660491714678	5.426356028283614E-4
50-51	7.23514137104482E-5	3.61757068552241E-5	0.0	1.1609688722512792	5.426356028283614E-4
52-53	7.23514137104482E-5	3.61757068552241E-5	0.0	1.3518499894728693	6.149870165388096E-4
54-55	7.23514137104482E-5	3.61757068552241E-5	0.0	1.57514454003674	6.511627233940337E-4
56-57	7.23514137104482E-5	3.61757068552241E-5	0.0	1.8352478723258012	7.235141371044819E-4
58-59	7.23514137104482E-5	3.61757068552241E-5	0.0	2.101410635513113	7.235141371044819E-4
60-61	7.23514137104482E-5	3.61757068552241E-5	0.0	2.382929986260467	7.596898439597061E-4
62-63	7.23514137104482E-5	3.61757068552241E-5	0.0	2.669839517329249	8.320412576701543E-4
64-65	7.23514137104482E-5	3.61757068552241E-5	0.0	2.9852012418396647	8.320412576701543E-4
66-67	7.23514137104482E-5	3.61757068552241E-5	0.0	3.355097844434331	8.320412576701543E-4
68-69	7.23514137104482E-5	3.61757068552241E-5	0.0	3.7563045213121944	8.320412576701543E-4
70-71	7.23514137104482E-5	3.61757068552241E-5	0.0	4.170950473286773	9.224805248082145E-4
72-73	7.23514137104482E-5	3.61757068552241E-5	0.0	4.60636128099625	0.0010129197919462748
74-75	7.23514137104482E-5	3.61757068552241E-5	0.0	5.058539528833124	0.001049095498801499
76-77	7.23514137104482E-5	3.61757068552241E-5	0.0	5.571022679997656	0.001049095498801499
78-79	7.23514137104482E-5	3.61757068552241E-5	0.0	6.142761638991045	0.001049095498801499
80-81	7.23514137104482E-5	3.61757068552241E-5	0.0	6.725715067109554	0.001049095498801499
82-83	7.23514137104482E-5	3.61757068552241E-5	0.0	7.330301567927487	0.0010852712056567228
84-85	7.23514137104482E-5	3.61757068552241E-5	0.0	7.95836610249446	0.001103359059084335
86-87	7.23514137104482E-5	3.61757068552241E-5	0.0	8.637474559434153	0.001139534765939559
88-89	7.23514137104482E-5	3.61757068552241E-5	0.0	9.371117894458099	0.0011937983262223953
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2125	0.0	45.565933	1
GTATCAA	3645	0.0	40.10719	1
TCAACGC	4335	0.0	32.079815	4
CAACGCA	4375	0.0	31.568398	5
ATCAACG	4475	0.0	31.289457	3
TATCAAC	4765	0.0	29.685612	2
AACGCAG	4745	0.0	29.389305	6
GTACATG	6715	0.0	27.849617	1
TACATGG	7090	0.0	25.976349	2
ACATGGG	7295	0.0	24.334105	3
ACGCAGA	5565	0.0	24.296535	7
CGCAGAG	5830	0.0	23.031061	8
CATGGGG	6100	0.0	22.253048	4
GAGTACA	5115	0.0	22.08515	1
GCAGAGT	6590	0.0	19.655369	9
AGAGTAC	5520	0.0	19.039103	10-11
CGTATAC	200	1.2941129E-5	18.985806	3
AGTACAT	5750	0.0	17.666014	2
ATAGGAC	815	0.0	16.889212	3
GTATATG	965	0.0	16.723248	1
>>END_MODULE