FastQCFastQC Report
Mon 15 Aug 2016
SRR2921660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764286
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.051.7290231
GTATCAA40300.038.8085631
TCAACGC47400.031.9404684
CAACGCA49200.030.7021165
ATCAACG49850.030.4658833
TATCAAC50900.030.2103772
AACGCAG52700.028.930346
GTACATG61550.027.0319081
TACATGG65600.025.5387612
ACGCAGA63450.023.8033927
ACATGGG68200.023.5908873
CGCAGAG64550.023.1034458
TACACCG2903.6379788E-1222.9317045
CATGGGG56500.022.7640634
AGAGTAC60950.018.70169310-11
GCAGAGT73900.018.6378979
CCCTATA4101.8189894E-1218.5210272
TAAGGTG15950.018.4644895
TCTATAC8250.017.8335063
GAGTACA46700.017.2030931
Produced by FastQC (version 0.11.5)