##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2764286
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431637319727407	33.0	31.0	34.0	29.0	34.0
2	31.56176350782806	34.0	31.0	34.0	29.0	34.0
3	31.632108978593386	34.0	31.0	34.0	28.0	34.0
4	35.12051321751802	37.0	35.0	37.0	32.0	37.0
5	34.869637584533585	37.0	35.0	37.0	32.0	37.0
6	34.853750299353976	37.0	35.0	37.0	32.0	37.0
7	34.73602405829209	37.0	35.0	37.0	32.0	37.0
8	34.68466323672732	37.0	35.0	37.0	32.0	37.0
9	36.2867655517555	39.0	37.0	39.0	32.0	39.0
10-11	36.17219292793872	39.0	37.0	39.0	32.0	39.0
12-13	36.09957526102581	39.0	37.0	39.0	32.0	39.0
14-15	37.34796562294929	40.0	38.0	41.0	32.0	41.0
16-17	37.31132107893322	40.0	38.0	41.0	31.5	41.0
18-19	37.275008266149015	40.0	38.0	41.0	31.0	41.0
20-21	37.21431809154335	40.0	38.0	41.0	31.0	41.0
22-23	37.12845179550886	40.0	38.0	41.0	31.0	41.0
24-25	37.038483536074054	40.0	37.5	41.0	31.0	41.0
26-27	36.90647783912374	40.0	37.0	41.0	30.0	41.0
28-29	36.78229079769604	40.0	37.0	41.0	30.0	41.0
30-31	36.619571563868575	40.0	37.0	41.0	30.0	41.0
32-33	36.5585156528666	40.0	37.0	41.0	30.0	41.0
34-35	36.67697626077765	40.0	37.0	41.0	30.0	41.0
36-37	36.6661716624112	40.0	37.0	41.0	30.0	41.0
38-39	36.55104681642927	40.0	37.0	41.0	30.0	41.0
40-41	36.46024145113783	40.0	37.0	41.0	30.0	41.0
42-43	36.32222733103593	40.0	36.5	41.0	29.0	41.0
44-45	36.17914246210414	40.0	36.0	41.0	28.5	41.0
46-47	36.011480903206106	40.0	36.0	41.0	28.0	41.0
48-49	35.85681546699582	40.0	35.5	41.0	28.0	41.0
50-51	35.69315801621106	39.5	35.0	41.0	27.0	41.0
52-53	35.50935304812889	39.0	35.0	41.0	27.0	41.0
54-55	35.28730529330178	39.0	35.0	41.0	26.0	41.0
56-57	35.05718022664804	39.0	35.0	41.0	26.0	41.0
58-59	34.812857461203365	38.5	35.0	40.5	26.0	41.0
60-61	34.531097542005426	38.0	34.0	40.0	26.0	41.0
62-63	34.224380726162195	37.0	34.0	40.0	25.0	41.0
64-65	33.88704515379378	37.0	34.0	40.0	24.0	41.0
66-67	33.537618574923144	36.0	34.0	39.0	23.0	41.0
68-69	33.17922222953776	36.0	33.5	39.0	22.5	41.0
70-71	32.78084901489933	35.0	33.0	38.5	22.0	40.0
72-73	32.41844946579334	35.0	33.0	37.5	21.0	40.0
74-75	32.0249469483259	35.0	33.0	37.0	20.0	39.0
76-77	29.321675108870792	33.0	29.0	34.5	13.0	36.5
78-79	30.89255959766826	34.5	31.5	36.0	16.0	37.5
80-81	31.08283368652882	35.0	32.0	35.5	18.0	37.0
82-83	30.90415011326614	35.0	32.0	35.0	16.0	37.0
84-85	30.701580986916696	35.0	32.0	35.0	13.0	36.5
86-87	30.488748450775354	35.0	32.0	35.0	8.5	36.0
88-89	30.21173442255975	35.0	32.0	35.0	2.0	36.0
90-91	30.00849586475495	34.5	31.0	35.0	2.0	35.0
92-93	29.844454951477523	34.0	31.0	35.0	2.0	35.0
94-95	29.73390542802011	34.0	31.0	35.0	2.0	35.0
96-97	29.524083072446196	34.0	31.0	35.0	2.0	35.0
98-99	29.312976479278916	34.0	31.0	35.0	2.0	35.0
100-101	28.184149903447036	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	47585.0
3	16694.0
4	9160.0
5	6754.0
6	5913.0
7	5922.0
8	6343.0
9	6785.0
10	7549.0
11	8416.0
12	8962.0
13	9812.0
14	10434.0
15	11412.0
16	12317.0
17	13275.0
18	12205.0
19	10979.0
20	10681.0
21	10326.0
22	10866.0
23	12621.0
24	14370.0
25	17415.0
26	20626.0
27	25134.0
28	29951.0
29	36262.0
30	43909.0
31	54374.0
32	69228.0
33	91167.0
34	129389.0
35	194923.0
36	346502.0
37	660808.0
38	684905.0
39	90312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02577574349483	19.665454503290814	13.058898385537523	26.249871367676835
2	15.328011391035421	21.060139009123173	37.12385761350428	26.48799198633712
3	17.666016999405567	24.413535703418876	29.71104339070594	28.20940390646962
4	12.97104068240188	16.685388726028076	35.31497739424388	35.02859319732616
5	13.784869438285922	37.46574616282844	34.746354248271885	14.003030150613755
6	33.974463793198254	35.81703706490627	16.38433387646189	13.824165265433583
7	28.536131404685115	31.972028933340805	21.215829771532242	18.276009890441834
8	26.553583818751026	35.03613591357768	19.893925592359114	18.516354675312176
9	26.469856208696356	14.880851605696085	19.378471965977447	39.27082021963011
10-11	25.58002295371869	25.205025351517275	28.236078019479898	20.97887367528414
12-13	25.873501777680662	22.763012810587654	27.853441552146023	23.510043859585654
14-15	22.99749089996757	24.50949907177227	24.879144080515502	27.61386594774466
16-17	23.3414746776337	27.5605396041762	25.850224226115575	23.247761492074535
18-19	23.47976737253676	26.740310565477483	27.09337222998805	22.686549831997706
20-21	23.853398194175185	26.01823189210054	27.01436939930405	23.114000514420226
22-23	23.751449741452223	25.886449520780413	26.70005925580783	23.662041481959537
24-25	23.613835606353714	26.124169695943618	26.714360934256327	23.547633763446346
26-27	23.41472158842399	26.252523787180298	26.721122466218965	23.611632158176747
28-29	23.549726667620554	26.503264775785322	26.579256260253015	23.367752296341106
30-31	23.7073079658992	26.215407304275335	26.443914659712004	23.63337007011346
32-33	23.419402526611478	26.30725569558518	26.318688654127314	23.954653123676028
34-35	23.93370317260153	26.269942385899242	26.37853526066541	23.417819180833817
36-37	23.9827753339106	26.034598743820712	26.40134463242294	23.581281289845744
38-39	23.75141356574537	26.133800916403004	26.47520914984918	23.639576368002444
40-41	23.896310809989494	26.06127160854704	26.46424767671038	23.578169904753086
42-43	23.73803765757966	26.073063019925375	26.24854899814167	23.940350324353297
44-45	23.667476753346243	26.097356922623305	26.425564128746803	23.80960219528365
46-47	23.887776247091374	26.12890132089233	26.206084816105395	23.777237615910906
48-49	23.581430770594437	26.29387833366799	26.1691331694425	23.95555772629507
50-51	23.680625220904222	26.287503070500872	26.249644111312936	23.782227597281967
52-53	23.97568367945695	26.131145192483707	26.136227974910952	23.756943153148395
54-55	23.408882150285095	26.10066374282652	26.401983522738988	24.0884705841494
56-57	23.502695189359688	26.23493107952468	26.256330515831333	24.006043215284297
58-59	23.438035085992755	26.06714181021406	26.394346470786488	24.100476633006696
60-61	23.400378097958523	26.075355129348683	26.51320132043051	24.011065452262283
62-63	23.483632649273073	26.117258324246844	26.433752207397387	23.965356819082693
64-65	23.22053848749775	25.985536709669304	26.3995490739459	24.394375728887045
66-67	23.191846636495033	26.106614002046598	26.33306414627463	24.368475215183743
68-69	23.465625116497577	25.94623177615961	26.330786884806834	24.25735622253598
70-71	23.292290505462823	25.856764355909483	26.270245536578894	24.5806996020488
72-73	23.28133074327601	25.99680175205219	26.200519072964056	24.521348431707743
74-75	23.25065671669335	26.164725660773964	26.130078303028505	24.454539319504182
76-77	23.307790062960805	25.881841073361215	26.25895328790293	24.551415575775046
78-79	23.28878989408163	25.854208865325322	26.29476150399776	24.562239736595288
80-81	23.35594725999355	26.00918312936636	26.078541675037837	24.55632793560226
82-83	23.30206135261916	26.03530351093766	26.097108085091293	24.565527051351893
84-85	23.22905095872681	26.044823468818624	26.151632189998637	24.574493382455934
86-87	23.240560611117665	26.1760366114565	25.94421038989811	24.63919238752772
88-89	23.06648469458933	25.98682450077564	26.016957654483207	24.92973315015182
90-91	22.963564888997258	25.952206266545915	26.125321484139146	24.95890736031768
92-93	23.062553794323083	25.83403370013008	26.270523280771442	24.832889224775396
94-95	22.92619763192484	25.66641232669698	26.379016482170698	25.028373559207484
96-97	22.89182411076954	25.499224003202762	26.39156152553788	25.217390360489816
98-99	22.749132949649763	25.620447013428926	26.21534466585415	25.415075371067164
100-101	22.707700822907935	25.55321317557088	26.281792834126033	25.457293167395157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	43.0
2	58.0
3	86.5
4	79.5
5	83.5
6	146.0
7	307.5
8	450.5
9	484.0
10	427.0
11	357.5
12	352.0
13	407.0
14	438.5
15	477.0
16	558.0
17	610.5
18	718.5
19	841.0
20	967.5
21	1150.5
22	1334.0
23	1628.0
24	2115.5
25	2908.5
26	4436.0
27	6789.0
28	9875.0
29	14202.0
30	19483.0
31	25823.5
32	34217.5
33	38975.5
34	40658.5
35	44562.0
36	50320.0
37	55971.0
38	61436.0
39	67171.5
40	72787.5
41	79069.5
42	84840.5
43	90890.5
44	96645.5
45	103292.5
46	108631.5
47	111719.0
48	115237.0
49	116698.5
50	116537.5
51	116254.0
52	115637.0
53	115196.0
54	113571.5
55	107990.0
56	99733.5
57	90038.0
58	81000.5
59	71409.5
60	58887.5
61	48251.5
62	40417.5
63	31145.5
64	22212.0
65	16267.5
66	12242.5
67	9677.0
68	7407.0
69	5499.0
70	4151.5
71	2962.5
72	2071.0
73	1534.5
74	1134.0
75	816.0
76	560.0
77	347.0
78	208.5
79	117.5
80	69.0
81	43.0
82	29.0
83	20.5
84	17.0
85	15.0
86	9.0
87	7.0
88	6.5
89	3.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1621395181251144
2	2.532299479865687E-4
3	0.011105942004553797
4	0.006041343044822425
5	0.026299738883747918
6	0.0019534881701821012
7	3.61757068552241E-5
8	0.0
9	7.23514137104482E-5
10-11	0.04829456865172417
12-13	3.6175706855224096E-4
14-15	0.005335916761145555
16-17	1.808785342761205E-5
18-19	0.0013746768604985158
20-21	3.2558136169701687E-4
22-23	0.0
24-25	4.521963356903012E-4
26-27	0.003147286496404497
28-29	0.005806200950263468
30-31	0.01736433929050757
32-33	0.012552970278762761
34-35	0.010581394255153049
36-37	0.003997415607502263
38-39	0.0
40-41	0.0036899220992328576
42-43	0.008953487446667964
44-45	0.006457363673657501
46-47	0.0034005164443910653
48-49	0.009080102420661249
50-51	0.0028578808415627038
52-53	0.0018811367564716532
54-55	0.004087854874640323
56-57	0.007036174983341087
58-59	0.01517570902576651
60-61	0.006782945035354517
62-63	0.020529713640339675
64-65	0.03919637837763531
66-67	0.05958138919055409
68-69	0.04894573137511821
70-71	0.06826355883580787
72-73	0.03248578475599124
74-75	0.02631782673717553
76-77	0.22504907234634913
78-79	0.08322221362044305
80-81	0.06616536783820488
82-83	0.026643408098872546
84-85	0.05075451671787941
86-87	0.06772092323297951
88-89	0.29611624846343687
90-91	0.30090952962175405
92-93	0.05238242352636449
94-95	0.009731265144055283
96-97	0.015248060439476956
98-99	0.001049095498801499
100-101	1.2661497399328435E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2764286.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.639274880733375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34184082033717	48.112316950478174
2	11.483147141520162	13.926594320210976
3	3.655506291775621	6.650017525656966
4	1.7278698323875745	4.191070948971073
5	0.9820219147064608	2.9774548412394592
6	0.6018343991721572	2.1896880938448873
7	0.4171083422243427	1.7705203193432246
8	0.29933890035368094	1.452135508883465
9	0.22935554436143268	1.2517158509957838
>10	1.1986917359845222	12.595898202638189
>50	0.04656504532208039	1.891992684653179
>100	0.014384528357242497	1.5718567254955171
>500	0.0013773482165506017	0.5932198013697755
>1k	9.581552809802853E-4	0.8255182262192928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.61757068552241E-5
2	0.0	3.61757068552241E-5	0.0	0.0	3.61757068552241E-5
3	0.0	3.61757068552241E-5	0.0	0.0	3.61757068552241E-5
4	0.0	3.61757068552241E-5	0.0	0.0	3.61757068552241E-5
5	0.0	3.61757068552241E-5	0.0	0.0	3.61757068552241E-5
6	0.0	3.61757068552241E-5	0.0	0.0	1.447028274208964E-4
7	0.0	3.61757068552241E-5	0.0	0.0	1.447028274208964E-4
8	0.0	3.61757068552241E-5	0.0	3.61757068552241E-5	1.8087853427612048E-4
9	0.0	3.61757068552241E-5	0.0	3.61757068552241E-5	1.8087853427612048E-4
10-11	0.0	3.61757068552241E-5	0.0	3.61757068552241E-5	1.8087853427612048E-4
12-13	0.0	3.61757068552241E-5	0.0	9.043926713806024E-5	2.1705424113134457E-4
14-15	0.0	3.61757068552241E-5	0.0	1.447028274208964E-4	2.3514209455895662E-4
16-17	0.0	3.61757068552241E-5	0.0	1.6279068084850844E-4	3.4366921512462895E-4
18-19	0.0	3.61757068552241E-5	0.0	1.8087853427612048E-4	4.3410848226268915E-4
20-21	0.0	3.61757068552241E-5	0.0	1.8087853427612048E-4	4.3410848226268915E-4
22-23	0.0	3.61757068552241E-5	0.0	1.9896638770373253E-4	4.7028418911791324E-4
24-25	0.0	5.426356028283615E-5	0.0	3.2558136169701687E-4	5.426356028283614E-4
26-27	0.0	7.23514137104482E-5	0.0	5.245477494007495E-4	5.426356028283614E-4
28-29	0.0	7.23514137104482E-5	0.0	0.003165374349832109	5.426356028283614E-4
30-31	0.0	7.23514137104482E-5	0.0	0.015519378240891137	5.426356028283614E-4
32-33	0.0	7.23514137104482E-5	0.0	0.04426097733736668	5.426356028283614E-4
34-35	0.0	7.23514137104482E-5	0.0	0.08418086985210647	5.426356028283614E-4
36-37	0.0	7.23514137104482E-5	0.0	0.14558913223884937	5.426356028283614E-4
38-39	0.0	7.23514137104482E-5	0.0	0.244403075513894	5.426356028283614E-4
40-41	0.0	7.23514137104482E-5	0.0	0.35618600969653647	5.426356028283614E-4
42-43	0.0	7.23514137104482E-5	0.0	0.4735038270280282	5.426356028283614E-4
44-45	0.0	7.23514137104482E-5	0.0	0.6225658271249791	5.607234562559735E-4
46-47	0.0	7.23514137104482E-5	0.0	0.796480537831469	5.788113096835856E-4
48-49	0.0	7.23514137104482E-5	0.0	0.9732350415260939	5.788113096835856E-4
50-51	0.0	7.23514137104482E-5	0.0	1.1585450998919793	6.149870165388097E-4
52-53	0.0	7.23514137104482E-5	0.0	1.3481058038133535	6.692505768216458E-4
54-55	0.0	7.23514137104482E-5	0.0	1.5710205094552445	7.235141371044819E-4
56-57	0.0	7.23514137104482E-5	0.0	1.830219449072925	7.41601990532094E-4
58-59	0.0	7.23514137104482E-5	0.0	2.0958576645108358	7.596898439597061E-4
60-61	0.0	7.23514137104482E-5	0.0	2.377666420913031	7.596898439597061E-4
62-63	0.0	7.23514137104482E-5	0.0	2.6634183293624467	8.320412576701543E-4
64-65	0.0	7.23514137104482E-5	0.0	2.9781469790028963	8.320412576701543E-4
66-67	0.0	7.23514137104482E-5	0.0	3.3473019796070305	8.320412576701543E-4
68-69	0.0	7.23514137104482E-5	0.0	3.746609431874994	8.320412576701543E-4
70-71	0.0	7.23514137104482E-5	0.0	4.1605137818590405	8.682169645253783E-4
72-73	1.808785342761205E-5	7.23514137104482E-5	0.0	4.594893581923143	9.948319385186628E-4
74-75	7.23514137104482E-5	7.23514137104482E-5	0.0	5.047379323268287	0.0010129197919462748
76-77	7.23514137104482E-5	7.23514137104482E-5	0.0	5.559319838829991	0.0010129197919462748
78-79	7.23514137104482E-5	7.23514137104482E-5	0.0	6.131348203478222	0.0010129197919462748
80-81	7.23514137104482E-5	7.23514137104482E-5	0.0	6.715766747724367	0.0010129197919462748
82-83	7.23514137104482E-5	7.23514137104482E-5	0.0	7.3212214655068255	0.0010129197919462748
84-85	7.23514137104482E-5	7.23514137104482E-5	0.0	7.952885482905893	0.0010129197919462748
86-87	7.23514137104482E-5	7.23514137104482E-5	0.0	8.63682339671076	0.0010490954988014987
88-89	7.23514137104482E-5	7.23514137104482E-5	0.0	9.37299903121457	0.0010852712056567228
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2325	0.0	51.729023	1
GTATCAA	4030	0.0	38.808563	1
TCAACGC	4740	0.0	31.940468	4
CAACGCA	4920	0.0	30.702116	5
ATCAACG	4985	0.0	30.465883	3
TATCAAC	5090	0.0	30.210377	2
AACGCAG	5270	0.0	28.93034	6
GTACATG	6155	0.0	27.031908	1
TACATGG	6560	0.0	25.538761	2
ACGCAGA	6345	0.0	23.803392	7
ACATGGG	6820	0.0	23.590887	3
CGCAGAG	6455	0.0	23.103445	8
TACACCG	290	3.6379788E-12	22.931704	5
CATGGGG	5650	0.0	22.764063	4
AGAGTAC	6095	0.0	18.701693	10-11
GCAGAGT	7390	0.0	18.637897	9
CCCTATA	410	1.8189894E-12	18.521027	2
TAAGGTG	1595	0.0	18.464489	5
TCTATAC	825	0.0	17.833506	3
GAGTACA	4670	0.0	17.203093	1
>>END_MODULE