FastQCFastQC Report
Mon 15 Aug 2016
SRR2921659_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921659_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.055.250291
GTATCAA37150.040.8822171
TCAACGC43300.033.543034
CAACGCA43850.033.120375
ATCAACG44750.032.1367073
TATCAAC46900.031.1694892
AACGCAG47450.030.591426
GTACATG64250.028.4400841
TACATGG67500.026.5087572
ACGCAGA54550.025.9181357
ACATGGG68600.025.1842543
CGCAGAG58300.023.8464458
CATGGGG56800.022.3952124
TAAGGTA6100.020.2307624
GAGTACA47400.020.1261631
GCAGAGT67700.019.4841189
GTGTAGC12700.019.4331361
AGAGTAC53500.018.44735710-11
CAGAGTA61200.017.59944710-11
ATTATAC7600.016.8617253
Produced by FastQC (version 0.11.5)