##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921659_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2562853 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.542840342384054 31.0 31.0 34.0 27.0 34.0 2 30.596445835949233 33.0 31.0 34.0 27.0 34.0 3 30.653920455055363 33.0 31.0 34.0 26.0 34.0 4 34.12033854458293 37.0 35.0 37.0 30.0 37.0 5 34.120514520341196 37.0 35.0 37.0 30.0 37.0 6 34.17063678642513 37.0 35.0 37.0 30.0 37.0 7 34.07951763132728 37.0 35.0 37.0 30.0 37.0 8 34.054662518685234 37.0 35.0 37.0 30.0 37.0 9 35.630669414125585 39.0 37.0 39.0 30.0 39.0 10-11 35.533337846532746 39.0 37.0 39.0 30.0 39.0 12-13 35.42906284519635 39.0 37.0 39.0 30.0 39.0 14-15 36.59226924056901 40.0 37.0 41.0 30.0 41.0 16-17 36.53371594078942 40.0 37.0 41.0 29.5 41.0 18-19 36.502609201542185 40.0 37.0 41.0 29.0 41.0 20-21 36.42934553796101 40.0 37.0 41.0 29.0 41.0 22-23 36.32958874348236 40.0 37.0 41.0 28.5 41.0 24-25 36.213636131295864 40.0 37.0 41.0 27.5 41.0 26-27 36.06617956628804 40.0 36.0 41.0 27.0 41.0 28-29 35.93199278304296 40.0 36.0 41.0 27.0 41.0 30-31 35.77851402323894 40.0 36.0 41.0 27.0 41.0 32-33 35.59967426926164 40.0 35.5 41.0 26.0 41.0 34-35 35.42553220961171 39.0 35.0 41.0 25.0 41.0 36-37 35.379156159171046 39.0 35.0 41.0 25.0 41.0 38-39 35.55027639119372 40.0 35.5 41.0 25.5 41.0 40-41 35.60096092128577 40.0 35.5 41.0 26.0 41.0 42-43 35.429381825645095 40.0 35.0 41.0 25.0 41.0 44-45 35.29919897863826 40.0 35.0 41.0 24.0 41.0 46-47 35.13088577456452 40.0 35.0 41.0 23.5 41.0 48-49 34.952964333108454 39.5 35.0 41.0 23.0 41.0 50-51 33.24815001094483 37.0 32.5 39.5 19.0 40.0 52-53 33.35923402551765 37.5 33.5 39.5 19.5 40.0 54-55 34.26350711492232 39.0 34.0 40.5 21.0 41.0 56-57 34.27049639600867 39.0 34.0 41.0 20.5 41.0 58-59 34.074686101777985 38.0 34.0 41.0 20.0 41.0 60-61 33.81735511166657 38.0 34.0 40.0 18.5 41.0 62-63 33.519524920079306 37.0 34.0 40.0 18.0 41.0 64-65 33.219000075306695 37.0 33.5 40.0 16.0 41.0 66-67 32.875199826131265 36.0 33.0 39.0 13.0 41.0 68-69 32.51427315573699 36.0 33.0 39.0 9.5 41.0 70-71 32.10327338321784 35.0 33.0 39.0 7.0 40.5 72-73 31.743561374764766 35.0 32.0 37.5 4.0 40.0 74-75 31.35207325585978 35.0 32.0 37.0 2.0 39.0 76-77 30.980386701851415 35.0 32.0 36.5 2.0 39.0 78-79 30.636336145693882 35.0 32.0 36.0 2.0 38.5 80-81 30.323932937238304 35.0 31.0 35.5 2.0 37.0 82-83 30.010183962950663 35.0 31.0 35.0 2.0 37.0 84-85 29.74737821482543 35.0 31.0 35.0 2.0 36.5 86-87 29.50495795115834 35.0 31.0 35.0 2.0 36.0 88-89 29.266930643310403 35.0 31.0 35.0 2.0 36.0 90-91 29.0310179709878 35.0 30.0 35.0 2.0 35.5 92-93 28.82708860008748 34.0 30.0 35.0 2.0 35.0 94-95 28.58351317847727 34.0 30.0 35.0 2.0 35.0 96-97 28.33140468844682 34.0 30.0 35.0 2.0 35.0 98-99 28.040817986829524 34.0 29.5 35.0 2.0 35.0 100-101 26.94485969347442 33.0 26.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80513.0 3 14072.0 4 9816.0 5 7524.0 6 6969.0 7 7357.0 8 7766.0 9 8190.0 10 8880.0 11 9594.0 12 9689.0 13 9918.0 14 10428.0 15 11258.0 16 11318.0 17 12224.0 18 11289.0 19 10725.0 20 10684.0 21 11323.0 22 12339.0 23 14286.0 24 16355.0 25 19481.0 26 22998.0 27 27103.0 28 32413.0 29 38325.0 30 45345.0 31 55953.0 32 69385.0 33 89230.0 34 123749.0 35 182496.0 36 309258.0 37 556137.0 38 575507.0 39 102941.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41719070365922 17.80627470209141 12.451415641625845 25.325118952623516 2 16.445024072345895 19.420233265522075 38.47268481821767 25.66205784391436 3 19.17509116260582 22.995037646183274 29.55562158664908 28.274249604561817 4 13.505383598999185 15.18477130246549 35.83154504761994 35.47830005091539 5 14.309369334968494 36.791259780018635 34.445226624686626 14.454144260326244 6 34.48616303876581 34.76439684335359 16.466602382820838 14.282837735059761 7 29.245869856882063 31.48223884313066 20.906520268081742 18.365371031905536 8 26.889160508396788 34.27799163911446 19.951896809563504 18.880951042925247 9 26.884918118634815 14.989750927840523 19.243788999836497 38.88154195368816 10-11 25.83682585979175 24.726228787523723 27.997063799721793 21.439881552962728 12-13 26.224068592450156 22.389472281257458 27.55014168131354 23.836317444978846 14-15 23.305410838149133 24.075281633746044 24.63079088927347 27.98851663883135 16-17 23.65369431268615 27.118628522005622 25.702273996630158 23.52540316867807 18-19 23.75733296183172 26.343191003729455 26.925632947822365 22.973843086616462 20-21 24.11040931162681 25.723958378052238 26.79459720204722 23.371035108273734 22-23 24.07446549600499 25.563915937662294 26.538120668593763 23.82349789773895 24-25 23.873207591947 25.7448472385211 26.534596771250353 23.847348398281547 26-27 23.60370099706024 25.94067984306961 26.555639356706372 23.89997980316378 28-29 23.79652135330092 26.183156850123485 26.3595457517494 23.660776044826196 30-31 23.97826671045168 25.84855418717626 26.163244352581767 24.009934749790293 32-33 23.680177319390502 25.968374009675692 26.095295514432078 24.25615315650173 34-35 24.202739496175415 25.949180502185687 26.11572376372943 23.732356237909467 36-37 24.26760235013408 25.69012996362423 26.19359634310865 23.848671343133038 38-39 23.963120693570705 25.789799815665905 26.237368002503576 24.00971148825982 40-41 24.128362506925967 25.69468398092726 26.349919229598644 23.827034282548134 42-43 24.0485271070762 25.69776047537716 26.062310150745322 24.191402266801322 44-45 23.869118548543476 25.76751299517724 26.281631762652708 24.081736693626578 46-47 24.135804966999206 25.78732057168404 26.024091957690015 24.052782503626734 48-49 23.844081270703196 25.924430007163036 25.955123132215817 24.276365589917955 50-51 23.95607815478363 25.96249350752931 26.10267418441178 23.978754153275283 52-53 24.227889427588366 25.681615577103535 26.142751016578043 23.94774397873006 54-55 23.61859371288769 25.67665972355942 26.425225080810144 24.279521482742748 56-57 23.71037221018307 25.85012868371176 26.241546120379137 24.19795298572603 58-59 23.77831395213572 25.5612251182198 26.282753487550043 24.37770744209444 60-61 23.54800570683473 25.66417619940214 26.552129808005326 24.235688285757803 62-63 23.725999471390168 25.6560313912917 26.51117118356993 24.106797953748206 64-65 23.494714066147775 25.639740901464908 26.371138672141512 24.494406360245804 66-67 23.51156271912788 25.66902729359632 26.304768164045818 24.514641823229987 68-69 23.754756467681823 25.490679024001956 26.389936325006364 24.364628183309854 70-71 23.615887720220005 25.42102083295463 26.309807299616484 24.653284147208886 72-73 23.60317631909883 25.603652911622582 26.226096822566635 24.56707394671195 74-75 23.593864662735903 25.8167945318056 26.23850904673714 24.350831758721355 76-77 23.704106954192227 25.471716738323263 26.35733951544567 24.466836792038837 78-79 23.652957020412465 25.56499431178681 26.319173765284326 24.4628749025164 80-81 23.82679816517349 25.655270042507595 26.179028543049203 24.338903249269705 82-83 23.73893506400569 25.681463972687673 26.205565506630506 24.37403545667613 84-85 23.660593225558152 25.753409284421252 26.24805392525939 24.337943564761204 86-87 23.776380763758358 25.835548922476676 26.042206875405277 24.34586343835969 88-89 23.623846483850873 25.63309690415565 26.13752129876621 24.60553531322727 90-91 23.601208419041146 25.614177667479044 26.195077661592997 24.58953625188681 92-93 23.797555729370355 25.47039499413375 26.348044583496282 24.384004692999607 94-95 23.73137159428452 25.294192415469425 26.425321138950647 24.54911485129541 96-97 23.85402787955092 25.06589912930427 26.3950677980255 24.68500519311931 98-99 23.707027030409503 25.21792030812336 26.285346541003985 24.789706120463155 100-101 23.90343667967614 25.053227241943947 26.38268113542845 24.660654942951467 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63.0 1 48.5 2 74.0 3 118.5 4 114.0 5 132.5 6 205.5 7 362.5 8 551.5 9 617.0 10 568.5 11 537.5 12 586.5 13 637.0 14 700.0 15 779.5 16 887.5 17 1011.5 18 1111.0 19 1256.0 20 1383.0 21 1548.5 22 1751.0 23 1916.5 24 2185.5 25 2685.0 26 3380.0 27 4470.0 28 6337.0 29 8922.5 30 12468.0 31 17528.0 32 25051.5 33 29532.0 34 31680.5 35 36404.0 36 42882.0 37 49834.5 38 56306.5 39 62269.0 40 67487.5 41 73076.0 42 78691.0 43 84326.0 44 89889.5 45 97207.0 46 102215.5 47 105117.5 48 108404.0 49 109200.0 50 108774.5 51 108939.0 52 109137.5 53 108653.5 54 107340.5 55 102621.5 56 94773.0 57 85335.0 58 77481.0 59 68375.5 60 56259.0 61 47024.5 62 39880.0 63 30796.5 64 22139.5 65 16515.0 66 12599.5 67 9919.5 68 7718.0 69 5995.0 70 4668.0 71 3451.5 72 2520.0 73 1881.0 74 1426.0 75 1128.0 76 860.5 77 582.0 78 393.0 79 248.5 80 175.5 81 140.5 82 95.5 83 77.5 84 67.0 85 60.0 86 51.5 87 40.5 88 41.0 89 38.0 90 26.5 91 22.5 92 19.5 93 13.0 94 15.0 95 14.5 96 11.0 97 9.0 98 6.0 99 5.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05185627111660325 2 0.07113166459410665 3 0.06906365679186438 4 0.06793210535290163 5 0.06383510876355374 6 0.010144943935528101 7 0.013695674312962938 8 0.01736346173580771 9 0.007101460754869671 10-11 0.01394929791135114 12-13 0.02296269040791649 14-15 0.04134064653727701 16-17 0.027781538777292336 18-19 0.01092532423826103 20-21 0.021499477340292245 22-23 0.037789916159842174 24-25 0.03517564214568686 26-27 0.022260348135456853 28-29 0.01301284154807162 30-31 0.012973822532934975 32-33 0.009579168216046726 34-35 0.006067456853748538 36-37 7.998898103012541E-4 38-39 0.0044871867407143525 40-41 0.001287627499509336 42-43 0.22340337116486977 44-45 0.19435371439563642 46-47 0.1994456958709688 48-49 0.2059033428760838 50-51 0.19831414443200607 52-53 0.20496688651280426 54-55 0.20135762761266449 56-57 0.2038938635965465 58-59 0.2120488377601056 60-61 0.2172773857884163 62-63 0.20293789772569867 64-65 0.20352318295274838 66-67 0.2001090191282918 68-69 0.19587545598596565 70-71 0.18785704837538478 72-73 0.19948471488610547 74-75 0.19821659689416446 76-77 0.192578349206919 78-79 0.2095906398064969 80-81 0.22096468271882935 82-83 0.21926735556038524 84-85 0.21310235116879508 86-87 0.2329825393809165 88-89 0.22552990748981702 90-91 0.23249480169170844 92-93 0.22837829559479222 94-95 0.23169491188140717 96-97 0.23819157790165882 98-99 0.23235823513873013 100-101 0.23904999623466502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2562853.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.08908191094314 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44288754386822 47.73650176865342 2 11.345341579898356 13.63462319004283 3 3.650491974138598 6.580641337477642 4 1.7288703459101682 4.155449273151868 5 0.9848466298653729 2.958926490584835 6 0.60652324596567 2.1867255004633357 7 0.4263613056104205 1.7933761591536854 8 0.2961003716130271 1.4233919586972712 9 0.23601748308823756 1.276386648633337 >10 1.21306831385351 12.747934338768188 >50 0.050231511213329215 1.9996069092251225 >100 0.016525354989549323 1.771386402986145 >500 0.001497376658847667 0.6019784844284845 >1k 0.0012369633268432807 1.1330715377336893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.803803027329308E-5 2 0.0 0.0 0.0 0.0 7.803803027329308E-5 3 0.0 0.0 0.0 0.0 7.803803027329308E-5 4 0.0 0.0 0.0 0.0 7.803803027329308E-5 5 0.0 0.0 0.0 0.0 7.803803027329308E-5 6 0.0 0.0 0.0 0.0 1.5607606054658616E-4 7 0.0 0.0 0.0 0.0 1.5607606054658616E-4 8 0.0 0.0 0.0 0.0 2.3411409081987926E-4 9 0.0 0.0 0.0 0.0 2.3411409081987926E-4 10-11 1.950950756832327E-5 0.0 0.0 0.0 2.3411409081987926E-4 12-13 3.901901513664654E-5 0.0 0.0 0.0 3.1215212109317233E-4 14-15 3.901901513664654E-5 0.0 0.0 0.0 3.1215212109317233E-4 16-17 7.803803027329308E-5 0.0 0.0 3.901901513664654E-5 3.316616286614956E-4 18-19 7.803803027329308E-5 0.0 0.0 7.803803027329308E-5 3.511711362298189E-4 20-21 7.803803027329308E-5 0.0 0.0 9.754753784161636E-5 3.511711362298189E-4 22-23 7.803803027329308E-5 0.0 0.0 2.14604583251556E-4 4.096996589347887E-4 24-25 7.803803027329308E-5 0.0 0.0 4.096996589347887E-4 4.4871867407143525E-4 26-27 7.803803027329308E-5 0.0 0.0 9.949848859844867E-4 5.267567043447283E-4 28-29 7.803803027329308E-5 0.0 0.0 0.004740810339102555 5.657757194813748E-4 30-31 7.803803027329308E-5 0.0 0.0 0.019958226242394704 6.243042421863447E-4 32-33 7.803803027329308E-5 0.0 0.0 0.0575920663416903 7.023422724596378E-4 34-35 7.803803027329308E-5 0.0 0.0 0.10874599518583392 7.413612875962843E-4 36-37 7.803803027329308E-5 0.0 0.0 0.18811067197377296 7.803803027329309E-4 38-39 7.803803027329308E-5 0.0 0.0 0.30908912840494557 7.803803027329309E-4 40-41 7.803803027329308E-5 0.0 0.0 0.44698232789785447 7.803803027329309E-4 42-43 7.803803027329308E-5 0.0 0.0 0.5960349657198443 8.193993178695774E-4 44-45 7.803803027329308E-5 0.0 0.0 0.7779025952717538 8.193993178695774E-4 46-47 7.803803027329308E-5 0.0 0.0 0.9976186695062104 8.389088254379007E-4 48-49 7.803803027329308E-5 0.0 0.0 1.2181931620736735 8.974373481428705E-4 50-51 7.803803027329308E-5 0.0 0.0 1.4428451417229158 9.36456363279517E-4 52-53 7.803803027329308E-5 0.0 0.0 1.6689993534549192 0.0010340039011211334 54-55 7.803803027329308E-5 0.0 0.0 1.9349919796414388 0.0010925324238261031 56-57 7.803803027329308E-5 0.0 0.0 2.2478659525146387 0.0010925324238261031 58-59 7.803803027329308E-5 3.901901513664654E-5 0.0 2.5663001350448114 0.0010925324238261031 60-61 7.803803027329308E-5 3.901901513664654E-5 0.0 2.8993274292360898 0.0011315514389627498 62-63 7.803803027329308E-5 3.901901513664654E-5 0.0 3.2355933016837097 0.0012095894692360429 64-65 9.754753784161636E-5 3.901901513664654E-5 0.0 3.604791222906659 0.0012095894692360429 66-67 1.1705704540993963E-4 3.901901513664654E-5 0.0 4.043969747777184 0.0012290989768043662 68-69 1.1705704540993963E-4 3.901901513664654E-5 0.0 4.513309971348337 0.0012486084843726893 70-71 1.1705704540993963E-4 3.901901513664654E-5 0.0 5.000267280253686 0.0013266465146459824 72-73 1.1705704540993963E-4 3.901901513664654E-5 0.0 5.505641564303533 0.0014046845449192755 74-75 1.1705704540993963E-4 3.901901513664654E-5 0.0 6.028223234028639 0.001443703560055922 76-77 1.1705704540993963E-4 3.901901513664654E-5 0.0 6.618288290432577 0.0014827225751925686 78-79 1.1705704540993963E-4 3.901901513664654E-5 0.0 7.270744752040011 0.0014827225751925686 80-81 1.1705704540993963E-4 3.901901513664654E-5 0.0 7.944310500836372 0.001502232082760892 82-83 1.1705704540993963E-4 3.901901513664654E-5 0.0 8.636780962466439 0.0015607606054658617 84-85 1.1705704540993963E-4 3.901901513664654E-5 0.0 9.356701301245135 0.0015607606054658617 86-87 1.1705704540993963E-4 3.901901513664654E-5 0.0 10.127443907239314 0.0015802701130341848 88-89 1.1705704540993963E-4 3.901901513664654E-5 0.0 10.949223385032228 0.0015997796206025081 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1950 0.0 55.25029 1 GTATCAA 3715 0.0 40.882217 1 TCAACGC 4330 0.0 33.54303 4 CAACGCA 4385 0.0 33.12037 5 ATCAACG 4475 0.0 32.136707 3 TATCAAC 4690 0.0 31.169489 2 AACGCAG 4745 0.0 30.59142 6 GTACATG 6425 0.0 28.440084 1 TACATGG 6750 0.0 26.508757 2 ACGCAGA 5455 0.0 25.918135 7 ACATGGG 6860 0.0 25.184254 3 CGCAGAG 5830 0.0 23.846445 8 CATGGGG 5680 0.0 22.395212 4 TAAGGTA 610 0.0 20.230762 4 GAGTACA 4740 0.0 20.126163 1 GCAGAGT 6770 0.0 19.484118 9 GTGTAGC 1270 0.0 19.433136 1 AGAGTAC 5350 0.0 18.447357 10-11 CAGAGTA 6120 0.0 17.599447 10-11 ATTATAC 760 0.0 16.861725 3 >>END_MODULE