FastQCFastQC Report
Mon 15 Aug 2016
SRR2921659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.049.9645081
GTATCAA33600.041.586321
TCAACGC43000.031.892224
ATCAACG44650.030.8205493
CAACGCA44150.030.7636815
TATCAAC45050.030.1255552
GTACATG63950.029.3561361
AACGCAG47400.028.5497066
TACATGG66700.026.9635582
ACATGGG68850.026.2594053
ACGCAGA54800.024.520687
CATGGGG57000.023.30984
CGCAGAG58650.022.5062648
ATGGGGG30050.018.9644285
AGAGTAC56300.018.59667610-11
GCAGAGT68500.018.1612559
GAGTACA46300.017.2452981
GTATAGC5650.016.8237931
CAGAGTA62600.016.61135110-11
ATAGGAC6700.016.2898433
Produced by FastQC (version 0.11.5)