##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2562853
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21300636439156	33.0	31.0	34.0	28.0	34.0
2	31.35038568345512	34.0	31.0	34.0	28.0	34.0
3	31.42131327860006	34.0	31.0	34.0	28.0	34.0
4	34.9011718580816	37.0	35.0	37.0	32.0	37.0
5	34.62508540286938	37.0	35.0	37.0	32.0	37.0
6	34.59255837147117	37.0	35.0	37.0	32.0	37.0
7	34.46840844948969	37.0	35.0	37.0	31.0	37.0
8	34.41013706209447	37.0	35.0	37.0	31.0	37.0
9	35.98238720675747	39.0	37.0	39.0	31.0	39.0
10-11	35.86503771382908	39.0	37.0	39.0	30.0	39.0
12-13	35.78375544754225	39.0	37.0	39.0	30.0	39.0
14-15	37.006283232007455	40.0	38.0	41.0	31.0	41.0
16-17	36.96661181893772	40.0	38.0	41.0	31.0	41.0
18-19	36.92399173889412	40.0	37.5	41.0	30.5	41.0
20-21	36.857229618710086	40.0	37.0	41.0	30.0	41.0
22-23	36.76180217905592	40.0	37.0	41.0	30.0	41.0
24-25	36.67213550679653	40.0	37.0	41.0	30.0	41.0
26-27	36.53569010786026	40.0	37.0	41.0	30.0	41.0
28-29	36.41053837266515	40.0	37.0	41.0	29.5	41.0
30-31	36.24569883641395	40.0	36.5	41.0	29.0	41.0
32-33	36.183016154262454	40.0	36.0	41.0	28.5	41.0
34-35	36.30218861557803	40.0	37.0	41.0	29.5	41.0
36-37	36.28221478172958	40.0	37.0	41.0	29.0	41.0
38-39	36.168639207945205	40.0	36.0	41.0	28.0	41.0
40-41	36.067405348648556	40.0	36.0	41.0	28.0	41.0
42-43	35.92936134066214	40.0	36.0	41.0	27.5	41.0
44-45	35.78116517022241	40.0	35.5	41.0	27.0	41.0
46-47	35.61265960240404	40.0	35.0	41.0	26.5	41.0
48-49	35.45000688685617	40.0	35.0	41.0	26.0	41.0
50-51	35.279008003970574	39.0	35.0	41.0	26.0	41.0
52-53	35.088060064311136	39.0	35.0	41.0	24.5	41.0
54-55	34.86652472849594	39.0	35.0	41.0	24.0	41.0
56-57	34.63140433727568	39.0	35.0	41.0	23.5	41.0
58-59	34.37813386097447	38.0	34.0	40.0	23.0	41.0
60-61	34.09751281091815	38.0	34.0	40.0	23.0	41.0
62-63	33.78960186167525	37.0	34.0	40.0	22.0	41.0
64-65	33.44527817241176	36.5	34.0	40.0	21.5	41.0
66-67	33.08917971495049	36.0	33.0	39.0	20.5	41.0
68-69	32.729384595995164	35.5	33.0	39.0	19.5	40.5
70-71	32.3344319787362	35.0	33.0	38.5	18.0	40.0
72-73	31.976482069006686	35.0	33.0	37.0	16.5	39.5
74-75	31.582052501645627	35.0	32.0	37.0	12.0	39.0
76-77	28.907633992273453	32.5	28.0	34.5	7.5	36.5
78-79	30.467233782039003	34.5	31.0	35.5	7.0	37.0
80-81	30.656127175456415	35.0	32.0	35.0	7.0	37.0
82-83	30.486303350211656	35.0	32.0	35.0	2.0	37.0
84-85	30.291759613212307	35.0	32.0	35.0	2.0	36.0
86-87	30.085974497952087	35.0	31.0	35.0	2.0	36.0
88-89	29.815049087871994	34.5	31.0	35.0	2.0	35.5
90-91	29.61892625133006	34.0	31.0	35.0	2.0	35.0
92-93	29.45672205936119	34.0	31.0	35.0	2.0	35.0
94-95	29.345281996275247	34.0	31.0	35.0	2.0	35.0
96-97	29.130873873764905	34.0	31.0	35.0	2.0	35.0
98-99	28.915434868874648	34.0	31.0	35.0	2.0	35.0
100-101	27.788459970197277	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56597.0
3	18968.0
4	10071.0
5	7304.0
6	6318.0
7	6301.0
8	6438.0
9	6959.0
10	7703.0
11	8320.0
12	8932.0
13	9193.0
14	9968.0
15	11097.0
16	11820.0
17	12301.0
18	11549.0
19	10456.0
20	10151.0
21	10028.0
22	10550.0
23	11832.0
24	14260.0
25	16866.0
26	19708.0
27	23650.0
28	28438.0
29	34144.0
30	41768.0
31	51717.0
32	65681.0
33	86291.0
34	122156.0
35	184454.0
36	325942.0
37	610274.0
38	602748.0
39	71900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.047772802787556	19.38088923748993	13.014699474245534	26.556638485476974
2	15.4061568908388	20.882633878195072	36.85175733908942	26.859451891876706
3	17.77904210684049	24.210418695836765	29.29797738567968	28.712561811643067
4	13.141723520588378	16.405614685715268	34.71647959892426	35.73618219477209
5	14.062285957149864	37.18927564094308	34.40854801526297	14.339890386644077
6	34.42478577083674	35.33551090548476	16.20934659958881	14.030356724089692
7	29.07830807241308	31.620280843372928	20.91459046406113	18.38682062015286
8	26.86884499423104	34.72032925805733	19.70034957135661	18.710476176355023
9	26.670649210586177	15.009222151424332	19.039850541447787	39.28027809654171
10-11	25.786322127105244	24.994271250343044	27.897761630142554	21.321644992409166
12-13	26.127008801933478	22.540917401982018	27.560902996122277	23.77117079996223
14-15	23.222375249465948	24.192709750892067	24.62297449452721	27.961940505114775
16-17	23.619790132325186	27.360016356771144	25.592903689755133	23.427289821148538
18-19	23.678747783199334	26.50491744429465	26.865458489980742	22.95087628252527
20-21	24.06836933173875	25.80901303044078	26.772532088362738	23.35008554945773
22-23	23.97704823491632	25.62811835091595	26.554741922381037	23.84009149178669
24-25	23.83249035492886	25.85261453369556	26.496549234978804	23.81834587639677
26-27	23.547037436994504	26.089615835974517	26.48067668901551	23.88267003801547
28-29	23.737490900655153	26.284234364394056	26.344912922367154	23.63336181258364
30-31	23.892831044303147	25.98791563776573	26.139589701461812	23.97966361646931
32-33	23.649406588260547	26.08851664138224	26.04980463509934	24.212272135257876
34-35	24.138544643214278	26.04362616789607	26.10463919486999	23.71318999401967
36-37	24.20782300873906	25.83125613599152	26.109629194911086	23.851291660358335
38-39	23.92589430607218	25.90285123649308	26.191962629148062	23.979291828286676
40-41	24.032532278839856	25.793982004466304	26.302635414264458	23.870850302429385
42-43	24.01715838827439	25.83433980008546	25.965866631285866	24.18263518035429
44-45	23.808117159628235	25.883618860499308	26.23598744662523	24.072276533247226
46-47	24.079453866849803	25.88314483103079	25.939139477396306	24.0982618247231
48-49	23.76370533486147	26.047482300182196	25.93542800526185	24.253384359694483
50-51	23.90273196153855	26.022856482858025	26.031109258951435	24.043302296651987
52-53	24.20997202656949	25.782711725389802	25.998433737619447	24.008882510421266
54-55	23.502023900502174	25.861540505617114	26.246537166430038	24.389898427450674
56-57	23.65483079317249	25.965188928730726	26.083270604881776	24.29670967321501
58-59	23.616034485550923	25.728805218569857	26.159933142488413	24.495227153390807
60-61	23.410373891603413	25.86560146910167	26.346626125371174	24.377398513923744
62-63	23.585336068910312	25.879360899071806	26.257460620141305	24.277842411876577
64-65	23.340053947075653	25.80895240400192	26.137045870628512	24.713947778293914
66-67	23.333533754504106	25.880880327868212	26.052513730476996	24.733072187150686
68-69	23.589948494005476	25.721297661393223	26.090098305847658	24.59865553875364
70-71	23.38466254015636	25.654757314533995	25.981142739447087	24.97943740586256
72-73	23.35680524403117	25.903313370402053	25.85425010982637	24.8856312757404
74-75	23.33679135852774	26.059986199122925	25.787012968765367	24.816209473583967
76-77	23.40839770108245	25.690655861788077	26.035516958261383	24.86542947886809
78-79	23.317807576158152	25.77952591702786	25.95820815553302	24.944458351280964
80-81	23.463143458165153	25.905617187843173	25.722669347793197	24.908570006198477
82-83	23.30340415899085	25.939773472382676	25.819581915259	24.937240453367473
84-85	23.186365285774603	25.88761245518043	25.895517916019024	25.03050434302595
86-87	23.156297983204595	26.094409959714852	25.652708241617184	25.09658381546337
88-89	23.002063474236312	25.8951733173567	25.725454224565325	25.37730898384166
90-91	22.87231180349816	25.940427293783213	25.75377707910318	25.43348382361545
92-93	23.003404615144973	25.8147399027844	25.891334959709518	25.29052052236111
94-95	22.83184200359792	25.571589680752677	26.005330544488192	25.591237771161207
96-97	22.848845048315752	25.370946312859115	26.012125426810456	25.768083212014677
98-99	22.628296598566195	25.544485765936187	25.866473442845038	25.96074419265258
100-101	22.559132434255783	25.542921178735075	25.86613569507997	26.031810691929174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	44.0
2	62.0
3	99.5
4	99.5
5	109.5
6	180.5
7	352.0
8	507.5
9	522.5
10	439.5
11	379.5
12	388.0
13	390.5
14	452.0
15	502.5
16	520.0
17	624.0
18	717.0
19	795.0
20	931.0
21	1066.0
22	1250.0
23	1531.0
24	1840.0
25	2325.5
26	3111.5
27	4358.0
28	6262.0
29	8988.5
30	12722.5
31	17892.5
32	25752.5
33	30199.0
34	31940.5
35	36641.0
36	43026.5
37	49379.0
38	55470.5
39	61580.5
40	67128.5
41	73252.5
42	79001.5
43	84406.5
44	90274.0
45	97436.0
46	102098.0
47	105479.5
48	109552.0
49	110450.5
50	110177.0
51	110025.5
52	110231.0
53	110555.5
54	108792.5
55	103171.5
56	95258.5
57	85824.5
58	77275.0
59	68277.0
60	56472.0
61	47054.0
62	39471.0
63	30241.0
64	21595.0
65	15873.0
66	12119.0
67	9619.5
68	7511.0
69	5650.0
70	4229.5
71	3062.0
72	2207.0
73	1677.5
74	1282.0
75	949.5
76	686.0
77	426.5
78	252.5
79	135.5
80	66.5
81	42.5
82	22.5
83	16.5
84	11.5
85	6.5
86	5.0
87	6.0
88	6.5
89	3.0
90	1.5
91	1.0
92	0.5
93	1.5
94	1.5
95	1.0
96	0.5
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15818308736396508
2	3.901901513664654E-5
3	0.008935354466292058
4	0.005657757194813749
5	0.022396914688435115
6	0.0023411409081987924
7	3.901901513664654E-5
8	0.0
9	7.803803027329308E-5
10-11	0.04729104634561561
12-13	2.731331059565258E-4
14-15	0.005013943445059081
16-17	0.0
18-19	0.0014827225751925686
20-21	3.1215212109317233E-4
22-23	0.0
24-25	6.047947346180213E-4
26-27	0.0031410307185000463
28-29	0.0065747040505249425
30-31	0.01757806631905927
32-33	0.013285974654028148
34-35	0.011139928821512587
36-37	0.004096996589347887
38-39	0.0
40-41	0.0041360156044845334
42-43	0.010066905905254808
44-45	0.007628217459214399
46-47	0.004214053634757827
48-49	0.009988867874981515
50-51	0.0030825021957950768
52-53	0.0024972169687453786
54-55	0.004038468066642917
56-57	0.008584183330062238
58-59	0.015158887380587181
60-61	0.007745274504624339
62-63	0.02208476256734194
64-65	0.04225759339298821
66-67	0.06165004391590153
68-69	0.05097834327602871
70-71	0.0690831662994327
72-73	0.03334174843426447
74-75	0.02719625355024264
76-77	0.22383258033137288
78-79	0.08435911072542981
80-81	0.0679125958453333
82-83	0.027040177489696053
84-85	0.051992837669581515
86-87	0.06771750076965007
88-89	0.29084773882856335
90-91	0.29414484560761
92-93	0.0518952901317399
94-95	0.01026200098093804
96-97	0.015080849350313888
98-99	8.58418333006224E-4
100-101	1.5607606054658616E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2562853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.86735127246957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14968559000705	47.38482030322474
2	11.482138576806554	13.748104470737035
3	3.775520158678927	6.78091174727764
4	1.722609661730765	4.125123108967427
5	0.9639258375052981	2.8853843357269553
6	0.6074296852368728	2.1819123803640896
7	0.4429880079717631	1.8564363077915962
8	0.3288345825462289	1.5749164371064779
9	0.2340702273292661	1.261184806975318
>10	1.2235620148649808	12.77953217392938
>50	0.051068387358479635	2.0544238595354236
>100	0.015550833179895907	1.7066010656261228
>500	0.0013736293115523817	0.5687567174271135
>1k	0.0012428074723397195	1.091892285310734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1705704540993963E-4
7	0.0	0.0	0.0	0.0	1.1705704540993963E-4
8	0.0	0.0	0.0	0.0	1.1705704540993963E-4
9	0.0	0.0	0.0	0.0	1.1705704540993963E-4
10-11	0.0	3.901901513664654E-5	0.0	0.0	1.1705704540993963E-4
12-13	0.0	3.901901513664654E-5	0.0	3.901901513664654E-5	1.365665529782629E-4
14-15	0.0	3.901901513664654E-5	0.0	3.901901513664654E-5	2.3411409081987926E-4
16-17	0.0	3.901901513664654E-5	0.0	3.901901513664654E-5	2.731331059565258E-4
18-19	0.0	3.901901513664654E-5	0.0	3.901901513664654E-5	2.731331059565258E-4
20-21	0.0	3.901901513664654E-5	0.0	5.852852270496981E-5	3.1215212109317233E-4
22-23	0.0	3.901901513664654E-5	0.0	1.5607606054658616E-4	3.316616286614956E-4
24-25	0.0	3.901901513664654E-5	0.0	3.316616286614956E-4	3.511711362298189E-4
26-27	0.0	3.901901513664654E-5	0.0	9.36456363279517E-4	4.29209166503112E-4
28-29	0.0	3.901901513664654E-5	0.0	0.004565224770987645	4.682281816397585E-4
30-31	0.0	3.901901513664654E-5	0.0	0.019646074121301534	4.682281816397585E-4
32-33	0.0	3.901901513664654E-5	0.0	0.05720187619032383	4.682281816397585E-4
34-35	0.0	3.901901513664654E-5	0.0	0.1082387479890575	4.682281816397585E-4
36-37	0.0	3.901901513664654E-5	0.0	0.1879741054207947	4.682281816397585E-4
38-39	0.0	3.901901513664654E-5	0.0	0.3079965959811195	5.07247196776405E-4
40-41	0.0	3.901901513664654E-5	0.0	0.4442705063458575	5.07247196776405E-4
42-43	0.0	3.901901513664654E-5	0.0	0.5921915927288846	5.07247196776405E-4
44-45	0.0	3.901901513664654E-5	0.0	0.7730642373948096	5.462662119130516E-4
46-47	0.0	3.901901513664654E-5	0.0	0.9913951365919154	5.852852270496981E-4
48-49	0.0	3.901901513664654E-5	0.0	1.2098235833268627	5.852852270496981E-4
50-51	0.0	3.901901513664654E-5	0.0	1.433558616120394	5.852852270496981E-4
52-53	0.0	3.901901513664654E-5	0.0	1.657040025315537	6.828327648913145E-4
54-55	0.0	3.901901513664654E-5	0.0	1.921062191237656	7.413612875962843E-4
56-57	0.0	3.901901513664654E-5	0.0	2.232004722861592	7.608707951646076E-4
58-59	0.0	3.901901513664654E-5	0.0	2.5472198366429915	7.803803027329309E-4
60-61	0.0	3.901901513664654E-5	0.0	2.877613347312546	7.803803027329309E-4
62-63	3.901901513664654E-5	3.901901513664654E-5	0.0	3.2113429837762837	7.803803027329309E-4
64-65	3.901901513664654E-5	3.901901513664654E-5	0.0	3.579448372575407	7.803803027329309E-4
66-67	3.901901513664654E-5	3.901901513664654E-5	0.0	4.016695456196668	7.803803027329309E-4
68-69	3.901901513664654E-5	3.901901513664654E-5	0.0	4.479597542270275	7.803803027329309E-4
70-71	3.901901513664654E-5	3.901901513664654E-5	0.0	4.962535892616549	7.803803027329309E-4
72-73	7.803803027329308E-5	3.901901513664654E-5	0.0	5.464866693485737	7.998898103012541E-4
74-75	7.803803027329308E-5	3.901901513664654E-5	0.0	5.987097192074613	8.779278405745472E-4
76-77	7.803803027329308E-5	3.901901513664654E-5	0.0	6.575406392797403	8.974373481428705E-4
78-79	7.803803027329308E-5	3.901901513664654E-5	0.0	7.226575226905328	8.974373481428705E-4
80-81	7.803803027329308E-5	3.901901513664654E-5	0.0	7.898170515437288	9.36456363279517E-4
82-83	7.803803027329308E-5	3.901901513664654E-5	0.0	8.588260817144018	0.0010535134086894567
84-85	7.803803027329308E-5	3.901901513664654E-5	0.0	9.307810475278917	0.0010535134086894567
86-87	7.803803027329308E-5	3.901901513664654E-5	0.0	10.077909267523342	0.0010925324238261033
88-89	7.803803027329308E-5	3.901901513664654E-5	0.0	10.90011795448276	0.0011315514389627498
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1950	0.0	49.964508	1
GTATCAA	3360	0.0	41.58632	1
TCAACGC	4300	0.0	31.89222	4
ATCAACG	4465	0.0	30.820549	3
CAACGCA	4415	0.0	30.763681	5
TATCAAC	4505	0.0	30.125555	2
GTACATG	6395	0.0	29.356136	1
AACGCAG	4740	0.0	28.549706	6
TACATGG	6670	0.0	26.963558	2
ACATGGG	6885	0.0	26.259405	3
ACGCAGA	5480	0.0	24.52068	7
CATGGGG	5700	0.0	23.3098	4
CGCAGAG	5865	0.0	22.506264	8
ATGGGGG	3005	0.0	18.964428	5
AGAGTAC	5630	0.0	18.596676	10-11
GCAGAGT	6850	0.0	18.161255	9
GAGTACA	4630	0.0	17.245298	1
GTATAGC	565	0.0	16.823793	1
CAGAGTA	6260	0.0	16.611351	10-11
ATAGGAC	670	0.0	16.289843	3
>>END_MODULE