FastQCFastQC Report
Mon 15 Aug 2016
SRR2921658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977964
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.040.7898561
GTATCAA31250.038.1197931
TCAACGC33200.034.3106654
CAACGCA34450.033.202385
ATCAACG35850.031.6409953
AACGCAG37550.030.4418666
TATCAAC39500.028.958492
GTACATG61700.028.6143951
TACATGG65450.026.0339932
ACGCAGA46000.024.5458537
ACATGGG66300.024.4823993
CGCAGAG48000.023.229728
GAGTACA46300.022.1411191
TACCGTA2806.730261E-1122.026447
CATGGGG55900.021.8211334
CCGTATG3252.1827873E-1120.4401429
GTACTAG6000.018.983961
AGTACAT51350.018.3012492
GTGTAGC18600.017.606091
GCAGAGT62050.017.129539
Produced by FastQC (version 0.11.5)