##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921658_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2977964
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.457896065902744	31.0	31.0	34.0	26.0	34.0
2	30.506112229697873	33.0	31.0	34.0	26.0	34.0
3	30.571716783681737	33.0	31.0	34.0	26.0	34.0
4	34.02794828950249	37.0	35.0	37.0	30.0	37.0
5	34.03148795620095	37.0	35.0	37.0	30.0	37.0
6	34.075939803167536	37.0	35.0	37.0	30.0	37.0
7	33.98768688943184	37.0	35.0	37.0	30.0	37.0
8	33.95867277106103	37.0	35.0	37.0	30.0	37.0
9	35.53481237516639	39.0	37.0	39.0	30.0	39.0
10-11	35.43345335940931	39.0	37.0	39.0	30.0	39.0
12-13	35.32742554980517	39.0	35.5	39.0	29.0	39.0
14-15	36.4829000619215	40.0	37.0	41.0	29.0	41.0
16-17	36.4240128490472	40.0	37.0	41.0	29.0	41.0
18-19	36.392954716712495	40.0	37.0	41.0	28.5	41.0
20-21	36.31748536919855	40.0	37.0	41.0	28.0	41.0
22-23	36.219645536346306	40.0	37.0	41.0	27.0	41.0
24-25	36.11632259490041	40.0	36.0	41.0	27.0	41.0
26-27	35.97153390705864	40.0	36.0	41.0	27.0	41.0
28-29	35.83599298043899	40.0	36.0	41.0	27.0	41.0
30-31	35.69397329853551	40.0	36.0	41.0	26.5	41.0
32-33	35.51983855412624	40.0	35.5	41.0	25.5	41.0
34-35	35.352583510076016	39.0	35.0	41.0	25.0	41.0
36-37	35.32222803902263	39.0	35.0	41.0	25.0	41.0
38-39	35.50120619322463	40.0	35.5	41.0	25.5	41.0
40-41	35.56884838097439	40.0	36.0	41.0	25.0	41.0
42-43	35.41087232753653	40.0	35.0	41.0	24.5	41.0
44-45	35.296693647068935	40.0	35.0	41.0	24.0	41.0
46-47	35.14921469836439	40.0	35.0	41.0	23.5	41.0
48-49	34.9920655521692	40.0	35.0	41.0	23.0	41.0
50-51	33.298892296884716	37.0	32.5	39.5	19.0	40.0
52-53	33.419376627789994	37.5	33.5	39.5	20.0	40.0
54-55	34.339012157299415	39.0	34.0	40.5	21.5	41.0
56-57	34.36415030537643	39.0	34.0	41.0	21.0	41.0
58-59	34.185022888120876	38.5	34.0	41.0	20.0	41.0
60-61	33.942849376285274	38.0	34.0	40.5	19.5	41.0
62-63	33.65877391398956	37.5	34.0	40.0	18.0	41.0
64-65	33.37381378686915	37.0	34.0	40.0	17.5	41.0
66-67	33.034187955260705	36.5	33.0	39.5	13.5	41.0
68-69	32.67516262788939	36.0	33.0	39.0	10.0	41.0
70-71	32.26622266085151	35.5	33.0	39.0	7.5	41.0
72-73	31.90168400289594	35.0	32.5	38.5	4.5	40.0
74-75	31.49851173486315	35.0	32.0	37.0	2.0	39.5
76-77	31.113730219707158	35.0	32.0	37.0	2.0	39.0
78-79	30.757030306612165	35.0	32.0	36.0	2.0	39.0
80-81	30.430340830177933	35.0	31.0	36.0	2.0	37.0
82-83	30.106276301526815	35.0	31.0	35.5	2.0	37.0
84-85	29.827544255068226	35.0	31.0	35.0	2.0	36.5
86-87	29.576936625157323	35.0	31.0	35.0	2.0	36.0
88-89	29.336142579292428	35.0	31.0	35.0	2.0	36.0
90-91	29.095659819930667	35.0	30.5	35.0	2.0	35.5
92-93	28.887479835216276	34.0	30.0	35.0	2.0	35.0
94-95	28.64417988263122	34.0	30.0	35.0	2.0	35.0
96-97	28.391158019371623	34.0	30.0	35.0	2.0	35.0
98-99	28.10501738771859	34.0	29.5	35.0	2.0	35.0
100-101	27.014637349544856	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98698.0
3	17142.0
4	11583.0
5	8860.0
6	8088.0
7	8352.0
8	8631.0
9	9273.0
10	10253.0
11	10785.0
12	10799.0
13	11159.0
14	11726.0
15	12664.0
16	12637.0
17	13087.0
18	12652.0
19	12006.0
20	12337.0
21	12811.0
22	14096.0
23	16317.0
24	18981.0
25	22169.0
26	26370.0
27	31453.0
28	37359.0
29	44115.0
30	52249.0
31	63543.0
32	78942.0
33	102968.0
34	141112.0
35	205656.0
36	345464.0
37	631065.0
38	701019.0
39	131526.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83995114961355	17.721065637247243	11.808321028344311	25.630662184794893
2	16.45241365219872	19.444473381156197	38.49887242035282	25.60424054629226
3	19.425707121586537	22.85948161554441	29.487173455898613	28.227637806970442
4	13.147067639259078	15.022930384910572	36.49005834748001	35.33994362835035
5	13.972143278117635	36.95219156973286	35.03400103357228	14.041664118577218
6	34.46907745865386	35.093794938720464	16.61025783281268	13.826869769813003
7	29.327798204452733	31.349736766805886	20.89174190653643	18.430723122204952
8	26.8115654849067	34.674254738906725	19.86733748001666	18.646842296169915
9	26.600337901257742	14.873666504018242	19.130342505338685	39.39565308938533
10-11	25.73193755292655	24.89688401052729	28.276223735711405	21.09495470083475
12-13	26.0978797282242	22.377924077026655	27.79908151897	23.725114675779153
14-15	23.14462146656553	24.123280271488166	24.86277109008465	27.869327171861656
16-17	23.664742249203776	27.124528664392926	25.750291047428135	23.460438038975166
18-19	23.59788304704743	26.559689502820323	26.874398020469652	22.968029429662597
20-21	24.03181071028649	25.903316425748702	26.845442374827112	23.2194304891377
22-23	23.860071027002622	25.836038612030787	26.476034097261042	23.827856263705552
24-25	23.693212479536765	25.90571905707972	26.64095945378303	23.760109009600484
26-27	23.595007284192672	26.1149768879074	26.519952810186954	23.77006301771297
28-29	23.674487467815872	26.31631886622536	26.43335969130776	23.575833974651005
30-31	23.92682355489619	26.056056938521742	26.281356026125092	23.735763480456978
32-33	23.646186987469992	26.175092349224222	26.225752399132013	23.952968264173773
34-35	24.049380817221866	26.12265917477221	26.316577764225624	23.5113822437803
36-37	24.088317995287685	25.892616834402666	26.340123645730685	23.67894152457896
38-39	23.768117304747165	26.12072029808078	26.38089118308659	23.730271214085462
40-41	24.007763109313665	25.897800502731677	26.486279649575433	23.608156738379222
42-43	23.805289110738496	25.93744031809038	26.298876066071397	23.95839450509973
44-45	23.735374262604793	25.97848216483034	26.45555913855317	23.830584434011698
46-47	23.884771497091734	25.968990813588245	26.240909139847172	23.905328549472852
48-49	23.642265994818644	26.118795700473235	26.27067987684394	23.968258427864185
50-51	23.749821892779703	26.137779203570116	26.360305986101668	23.752092917548513
52-53	23.93949115898451	25.92515515917832	26.331746145772154	23.803607536065012
54-55	23.450078453083844	25.87446127116034	26.612880840641573	24.062579435114245
56-57	23.556545736442455	26.003609602997507	26.47232301236309	23.96752164819695
58-59	23.627828878408913	25.813369315954443	26.54438968909468	24.014412116541966
60-61	23.529001020980235	25.887216344836915	26.65804969219162	23.925732941991228
62-63	23.62417396772567	25.87844445427658	26.621501291420635	23.875880286577114
64-65	23.43476282322501	25.796478749435376	26.559929477414517	24.208828949925095
66-67	23.415499977037147	25.836003501120302	26.48888875430806	24.259607767534494
68-69	23.590719866877794	25.76667312068927	26.524535743537893	24.118071268895047
70-71	23.447269639614362	25.63667815180159	26.50097324239421	24.415078966189835
72-73	23.46672431166655	25.78637480801355	26.457271765333264	24.289629114986635
74-75	23.47048996302277	25.89442387537053	26.445675803843876	24.18941035776282
76-77	23.53264640650245	25.662697114385853	26.4614240765303	24.3432324025814
78-79	23.466870980680348	25.73661729750974	26.496435272297553	24.300076449512364
80-81	23.58095355398275	25.815025946926962	26.392301113241878	24.21171938584841
82-83	23.626596616767557	25.84252704607221	26.38407105138114	24.14680528577909
84-85	23.602591378392223	25.822592707563064	26.40372638934106	24.17108952470365
86-87	23.58968110900345	25.865786295778232	26.32934074499894	24.215191850219377
88-89	23.522313688846662	25.73923648954607	26.355933672197914	24.38251614940936
90-91	23.54479773868649	25.731376970230986	26.40028325317755	24.323542037904968
92-93	23.720768063335658	25.59730976913305	26.469928502039565	24.211993665491722
94-95	23.698238852144087	25.493008988827576	26.51633457232055	24.29241758670779
96-97	23.727099555559594	25.334937485684723	26.520414824508393	24.417548134247287
98-99	23.5967892763621	25.352711612242807	26.48162099843819	24.568878112956906
100-101	23.84078598938248	25.25958568275507	26.518642929688585	24.38098539817387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	32.0
2	54.0
3	88.0
4	92.0
5	111.5
6	173.0
7	282.0
8	421.0
9	509.5
10	495.5
11	466.5
12	502.0
13	569.0
14	652.5
15	742.0
16	821.5
17	943.5
18	1088.5
19	1211.5
20	1322.0
21	1457.0
22	1638.0
23	1872.0
24	2211.5
25	2683.0
26	3424.5
27	4619.5
28	6470.0
29	9248.5
30	13209.0
31	18684.0
32	26628.5
33	32910.0
34	37578.5
35	44405.0
36	53169.0
37	62121.5
38	70004.5
39	77781.0
40	85084.0
41	92260.0
42	98679.0
43	104681.5
44	110853.5
45	117702.0
46	122829.0
47	125990.5
48	129442.5
49	130583.5
50	129799.5
51	129137.0
52	127399.5
53	124849.5
54	120610.5
55	113507.5
56	104711.0
57	94053.5
58	84485.0
59	73739.0
60	60539.5
61	49874.5
62	41179.5
63	31883.0
64	22820.0
65	16588.5
66	12713.5
67	9934.0
68	7681.0
69	5837.0
70	4509.5
71	3314.0
72	2409.5
73	1900.5
74	1473.0
75	1107.0
76	842.5
77	587.5
78	401.5
79	273.5
80	177.5
81	136.0
82	104.0
83	80.0
84	74.5
85	58.0
86	44.5
87	44.0
88	46.0
89	44.5
90	32.0
91	28.5
92	25.0
93	21.0
94	20.5
95	16.0
96	10.5
97	7.5
98	4.5
99	3.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05134380402180819
2	0.07122315783535328
3	0.06803305882811209
4	0.06715997909981451
5	0.06306322037472582
6	0.010006836885872361
7	0.012626076070765128
8	0.01625271494215511
9	0.00574217821303414
10-11	0.012441386128240637
12-13	0.022062053134289064
14-15	0.03982586760619
16-17	0.0263938717862271
18-19	0.00967103699037329
20-21	0.020215153709044167
22-23	0.035863428839300944
24-25	0.032858019774584246
26-27	0.021037863453016895
28-29	0.012441386128240637
30-31	0.012307066170041008
32-33	0.009150547152349727
34-35	0.0056414382443844184
36-37	6.883897857730987E-4
38-39	0.0038113288139144733
40-41	0.001124929649921893
42-43	0.21764870226772384
44-45	0.1877960915578563
46-47	0.1930177799328669
48-49	0.20022068769132198
50-51	0.19266519004259286
52-53	0.1993140279734745
54-55	0.19494862933198653
56-57	0.19785329842805355
58-59	0.20681915563787878
60-61	0.21188973405991476
62-63	0.1964261488721825
64-65	0.19699700869453088
66-67	0.19384048967683962
68-69	0.18997879087860028
70-71	0.18220502329779675
72-73	0.1930513599224168
74-75	0.19239655012619358
76-77	0.18625141203856058
78-79	0.2037633765888372
80-81	0.2154995829365298
82-83	0.21383737345380938
84-85	0.20774260535050124
86-87	0.22807528902297008
88-89	0.2206373213376656
90-91	0.22735331924764704
92-93	0.22231632081516095
94-95	0.22565752977537673
96-97	0.23252463763833278
98-99	0.2261108596343005
100-101	0.2327596975651821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2977964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.59022185607742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.47594485982711	53.44025299289591
2	10.808506127799609	14.178646297102976
3	3.305180597905101	6.503625860729946
4	1.4534164871869035	3.8131963937547884
5	0.7728801106133968	2.5346688961641175
6	0.4940160461381136	1.9441573239996635
7	0.32873286356126685	1.5093163016656985
8	0.23015399342933887	1.2076681192074017
9	0.18187802812227433	1.0736478193757275
>10	0.8995821211153152	10.123108007644866
>50	0.038216022617930216	1.672078040370324
>100	0.010105309536425959	1.1306998335829554
>500	7.707956376461468E-4	0.3439537889041784
>1k	6.166365096129068E-4	0.5249803246014797
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.71599790998145E-5
2	0.0	0.0	0.0	0.0	6.71599790998145E-5
3	0.0	0.0	0.0	0.0	6.71599790998145E-5
4	0.0	0.0	0.0	0.0	6.71599790998145E-5
5	0.0	0.0	0.0	0.0	6.71599790998145E-5
6	0.0	0.0	0.0	0.0	1.0073996864972175E-4
7	0.0	0.0	0.0	0.0	1.0073996864972175E-4
8	3.357998954990725E-5	0.0	0.0	0.0	1.0073996864972175E-4
9	3.357998954990725E-5	0.0	0.0	0.0	1.0073996864972175E-4
10-11	3.357998954990725E-5	0.0	0.0	0.0	1.0073996864972175E-4
12-13	3.357998954990725E-5	0.0	0.0	1.6789994774953625E-5	2.014799372994435E-4
14-15	3.357998954990725E-5	0.0	0.0	6.71599790998145E-5	2.518499216243044E-4
16-17	3.357998954990725E-5	0.0	0.0	6.71599790998145E-5	3.3579989549907253E-4
18-19	3.357998954990725E-5	0.0	0.0	6.71599790998145E-5	4.197498693738407E-4
20-21	3.357998954990725E-5	0.0	0.0	8.394997387476812E-5	4.365398641487943E-4
22-23	3.357998954990725E-5	0.0	0.0	1.1752996342467538E-4	4.7011985369870154E-4
24-25	3.357998954990725E-5	0.0	0.0	2.68639916399258E-4	4.7011985369870154E-4
26-27	3.357998954990725E-5	0.0	0.0	6.380198014482377E-4	4.7011985369870154E-4
28-29	3.357998954990725E-5	0.0	0.0	0.005960448145108537	4.7011985369870154E-4
30-31	6.71599790998145E-5	0.0	0.0	0.023741052611784427	5.036998432486088E-4
32-33	6.71599790998145E-5	0.0	0.0	0.06220693064120318	6.044398118983305E-4
34-35	6.71599790998145E-5	0.0	0.0	0.11088112549379374	6.044398118983305E-4
36-37	6.71599790998145E-5	0.0	0.0	0.18861880130182904	6.044398118983305E-4
38-39	6.71599790998145E-5	0.0	0.0	0.31200847290296324	6.212298066732842E-4
40-41	6.71599790998145E-5	0.0	0.0	0.44930026017775904	6.380198014482378E-4
42-43	6.71599790998145E-5	0.0	0.0	0.5958601245683293	6.380198014482378E-4
44-45	6.71599790998145E-5	0.0	0.0	0.7694518805465748	6.548097962231914E-4
46-47	6.71599790998145E-5	0.0	0.0	0.9732824171145118	6.883897857730987E-4
48-49	6.71599790998145E-5	0.0	0.0	1.181327242370962	7.051797805480523E-4
50-51	6.71599790998145E-5	0.0	0.0	1.4024178935675513	8.05919749197774E-4
52-53	6.71599790998145E-5	0.0	0.0	1.6274206135467049	9.234497126224495E-4
54-55	6.71599790998145E-5	5.0369984324860876E-5	0.0	1.8865573929033395	9.402397073974031E-4
56-57	6.71599790998145E-5	6.71599790998145E-5	0.0	2.190204448408376	9.570297021723567E-4
58-59	6.71599790998145E-5	6.71599790998145E-5	0.0	2.5037911808201843	9.738196969473103E-4
60-61	6.71599790998145E-5	6.71599790998145E-5	0.0	2.8325224885190017	9.738196969473103E-4
62-63	6.71599790998145E-5	6.71599790998145E-5	0.0	3.1684567039762737	0.0010073996864972175
64-65	6.71599790998145E-5	6.71599790998145E-5	0.0	3.5356874696940594	0.001024189681272171
66-67	6.71599790998145E-5	6.71599790998145E-5	0.0	3.9623883969047307	0.0010577696708220785
68-69	6.71599790998145E-5	6.71599790998145E-5	0.0	4.4144590062203575	0.001074559665597032
70-71	6.71599790998145E-5	6.71599790998145E-5	0.0	4.883823310154186	0.0011081396551469393
72-73	6.71599790998145E-5	6.71599790998145E-5	0.0	5.366753929866177	0.0012424596133465684
74-75	6.71599790998145E-5	6.71599790998145E-5	0.0	5.868136753835842	0.0012424596133465684
76-77	6.71599790998145E-5	6.71599790998145E-5	0.0	6.429174429240918	0.0012928295976714294
78-79	6.71599790998145E-5	6.71599790998145E-5	0.0	7.047365246859935	0.0013431995819962901
80-81	6.71599790998145E-5	6.71599790998145E-5	0.0	7.683185558992654	0.0013431995819962901
82-83	6.71599790998145E-5	6.71599790998145E-5	0.0	8.340866444322362	0.0013431995819962901
84-85	6.71599790998145E-5	6.71599790998145E-5	0.0	9.017755083674619	0.0013599895767712439
86-87	6.71599790998145E-5	6.71599790998145E-5	0.0	9.745433457221107	0.0013935695663211509
88-89	6.71599790998145E-5	6.71599790998145E-5	0.0	10.521433435729914	0.0014103595610961046
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1850	0.0	40.789856	1
GTATCAA	3125	0.0	38.119793	1
TCAACGC	3320	0.0	34.310665	4
CAACGCA	3445	0.0	33.20238	5
ATCAACG	3585	0.0	31.640995	3
AACGCAG	3755	0.0	30.441866	6
TATCAAC	3950	0.0	28.95849	2
GTACATG	6170	0.0	28.614395	1
TACATGG	6545	0.0	26.033993	2
ACGCAGA	4600	0.0	24.545853	7
ACATGGG	6630	0.0	24.482399	3
CGCAGAG	4800	0.0	23.22972	8
GAGTACA	4630	0.0	22.141119	1
TACCGTA	280	6.730261E-11	22.02644	7
CATGGGG	5590	0.0	21.821133	4
CCGTATG	325	2.1827873E-11	20.440142	9
GTACTAG	600	0.0	18.98396	1
AGTACAT	5135	0.0	18.301249	2
GTGTAGC	1860	0.0	17.60609	1
GCAGAGT	6205	0.0	17.12953	9
>>END_MODULE