FastQCFastQC Report
Mon 15 Aug 2016
SRR2921658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977964
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.040.6838651
GGTATCA18850.039.849161
TCAACGC36700.032.8520164
CAACGCA38100.031.6656025
ATCAACG41000.029.9859333
AACGCAG41500.029.0634966
TATCAAC42650.028.380682
GTACATG58050.025.0606671
ACGCAGA50600.023.3650787
TACATGG60650.023.0883482
CGCAGAG54200.021.7255528
ACATGGG65900.021.1049273
CATGGGG53650.019.1107794
AGAGTAC52150.018.0272410-11
GCAGAGT65650.017.2131619
GAGTACA46950.017.1129361
GTATAGA8000.016.6395451
TAAGGTG14300.016.2756775
GTAAGGT11500.016.09764
GTATATA8600.016.0314561
Produced by FastQC (version 0.11.5)