##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2977964
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15179263416213	33.0	31.0	34.0	28.0	34.0
2	31.28666800538892	33.0	31.0	34.0	28.0	34.0
3	31.36428949443311	34.0	31.0	34.0	28.0	34.0
4	34.840605192003665	37.0	35.0	37.0	32.0	37.0
5	34.55755408728917	37.0	35.0	37.0	32.0	37.0
6	34.529136013732874	37.0	35.0	37.0	31.0	37.0
7	34.39929730513868	37.0	35.0	37.0	31.0	37.0
8	34.339599471316646	37.0	35.0	37.0	30.0	37.0
9	35.913426085741804	39.0	37.0	39.0	30.0	39.0
10-11	35.79265095212702	39.0	37.0	39.0	30.0	39.0
12-13	35.709670936250404	39.0	37.0	39.0	30.0	39.0
14-15	36.92954448072575	40.0	37.0	41.0	31.0	41.0
16-17	36.88833377435053	40.0	37.0	41.0	30.0	41.0
18-19	36.84356593968228	40.0	37.0	41.0	30.0	41.0
20-21	36.78298831013404	40.0	37.0	41.0	30.0	41.0
22-23	36.692009372846684	40.0	37.0	41.0	30.0	41.0
24-25	36.603296581154105	40.0	37.0	41.0	30.0	41.0
26-27	36.474607483502155	40.0	37.0	41.0	29.5	41.0
28-29	36.34993388100058	40.0	37.0	41.0	29.0	41.0
30-31	36.19029477858027	40.0	36.0	41.0	28.5	41.0
32-33	36.13744659102662	40.0	36.0	41.0	28.5	41.0
34-35	36.266035620309715	40.0	37.0	41.0	29.0	41.0
36-37	36.26142122604571	40.0	37.0	41.0	29.0	41.0
38-39	36.15631837725372	40.0	36.5	41.0	28.0	41.0
40-41	36.07381721202809	40.0	36.0	41.0	28.0	41.0
42-43	35.94997471426787	40.0	36.0	41.0	27.5	41.0
44-45	35.82025118503783	40.0	36.0	41.0	27.0	41.0
46-47	35.668104114086	40.0	35.5	41.0	26.5	41.0
48-49	35.524978475226696	40.0	35.0	41.0	26.0	41.0
50-51	35.36966766555942	39.5	35.0	41.0	26.0	41.0
52-53	35.1951494376695	39.0	35.0	41.0	25.5	41.0
54-55	34.985710874946776	39.0	35.0	41.0	24.0	41.0
56-57	34.760989891079944	39.0	35.0	41.0	24.0	41.0
58-59	34.523172375488755	38.5	34.5	40.0	23.5	41.0
60-61	34.249835961751046	38.0	34.0	40.0	23.0	41.0
62-63	33.948159380032806	37.5	34.0	40.0	22.5	41.0
64-65	33.61770474727028	37.0	34.0	40.0	22.0	41.0
66-67	33.269664106080526	36.5	33.5	39.0	21.0	41.0
68-69	32.90691139986917	36.0	33.0	39.0	20.0	41.0
70-71	32.508841443348544	35.0	33.0	39.0	18.5	40.0
72-73	32.147024443545995	35.0	33.0	37.5	18.0	40.0
74-75	31.741138408657726	35.0	32.5	37.0	14.5	39.0
76-77	29.034769560679713	33.0	28.5	34.5	7.5	36.5
78-79	30.593742906227206	34.5	31.0	36.0	8.0	37.5
80-81	30.769864746518092	35.0	32.0	36.0	7.0	37.0
82-83	30.587876314152894	35.0	32.0	35.0	6.0	37.0
84-85	30.378943969772635	35.0	32.0	35.0	2.0	36.5
86-87	30.16129543540486	35.0	31.0	35.0	2.0	36.0
88-89	29.88299623501157	34.5	31.0	35.0	2.0	36.0
90-91	29.675534190473762	34.0	31.0	35.0	2.0	35.0
92-93	29.512175936310847	34.0	31.0	35.0	2.0	35.0
94-95	29.394856183620757	34.0	31.0	35.0	2.0	35.0
96-97	29.180036427572663	34.0	31.0	35.0	2.0	35.0
98-99	28.960782266004557	34.0	31.0	35.0	2.0	35.0
100-101	27.832805903630803	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69084.0
3	22787.0
4	11897.0
5	8335.0
6	7334.0
7	7036.0
8	7353.0
9	7822.0
10	8643.0
11	9391.0
12	10056.0
13	10609.0
14	11195.0
15	12051.0
16	12799.0
17	13463.0
18	12797.0
19	11711.0
20	11322.0
21	11479.0
22	12085.0
23	13580.0
24	15977.0
25	19058.0
26	22651.0
27	27351.0
28	32749.0
29	39604.0
30	47946.0
31	59629.0
32	75916.0
33	98840.0
34	139623.0
35	209295.0
36	364127.0
37	698013.0
38	734726.0
39	89627.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34672493430093	19.33368668792624	12.400392008356746	26.91919636941608
2	15.455613910193556	20.91938776880818	36.6513317841744	26.973666536823863
3	17.8656934610537	24.01033566391763	29.229080238080286	28.894890636948382
4	12.816940193500507	16.30495303532509	35.20335687443959	35.6747498967348
5	13.76321939612811	37.25372440510618	34.9652733693531	14.017782829412607
6	34.470197118774976	35.70170254205984	16.217065717451895	13.611034621713289
7	29.056899277492942	31.61149698250214	20.85901642867409	18.47258731133083
8	26.757576653042147	35.148578021762525	19.541068998819327	18.55277632637601
9	26.386954308379817	14.796082155459233	18.92088017182209	39.89608336433886
10-11	25.64146711666343	25.139504148001723	28.19312480556767	21.02590392976718
12-13	26.042607434062543	22.46857700478634	27.733365301585366	23.75545025956575
14-15	22.993487017270848	24.29759473494298	24.73469279471587	27.974225453070307
16-17	23.543451163277997	27.320729867788867	25.647069608631938	23.4887493603012
18-19	23.541867170284743	26.74788224524651	26.764168791753164	22.94608179271558
20-21	23.920140552734644	26.020422726050107	26.76079607596752	23.29864064524773
22-23	23.734105538902675	25.969609768554918	26.404000586306715	23.892284106235685
24-25	23.588909939938468	26.111101410166604	26.51251903157411	23.787469618320817
26-27	23.46222732128978	26.288560695647732	26.468268686533218	23.780943296529266
28-29	23.58490074983121	26.46667249870584	26.356221960246874	23.592204791216076
30-31	23.828419660423567	26.23614849365603	26.18971651809554	23.74571532782486
32-33	23.585099718013186	26.264604147975152	26.12481136370086	24.0254847703108
34-35	23.922984529974276	26.213674724276082	26.305558909231287	23.557781836518355
36-37	23.94578988417008	26.018504915026995	26.316286121817445	23.719419078985478
38-39	23.678744941174575	26.255035319433006	26.325821937404214	23.740397801988202
40-41	23.920724092156327	26.021331476066816	26.375915802425435	23.682028629351414
42-43	23.72185704465092	26.067073938635165	26.182078846787405	24.02899016992651
44-45	23.68376644583568	26.10141658138186	26.316191576407494	23.89862539637497
46-47	23.77909039453445	26.125060299346693	26.137015227253606	23.958834078865248
48-49	23.569484462130124	26.22219035546675	26.223399351810045	23.98492583059308
50-51	23.634827157413504	26.259309046057215	26.280784033489564	23.825079763039717
52-53	23.834058479712834	26.07434187362288	26.17355593505433	23.91804371160996
54-55	23.319154180572134	26.040785856622538	26.439832684430293	24.20022727837504
56-57	23.40729775420134	26.125787426563384	26.296974414807483	24.169940404427788
58-59	23.463627767419624	26.007216394956146	26.36239387370486	24.16676196391937
60-61	23.373354102961454	26.069420640548362	26.492257313460804	24.064967943029384
62-63	23.4375983997751	26.051734581789095	26.38763940125579	24.123027617180014
64-65	23.209215787022238	25.942236218790114	26.33024177449218	24.518306219695464
66-67	23.213982714135426	26.035678513696542	26.203560996966253	24.546777775201775
68-69	23.36264930610092	25.92147933253317	26.26142814786942	24.45444321349649
70-71	23.233582311856058	25.730894523262158	26.22868355567339	24.806839609208392
72-73	23.245788988294038	25.90784338457699	26.153880778431475	24.692486848697502
74-75	23.21362892978459	25.966044229295836	26.055038229296507	24.765288611623063
76-77	23.275900655692354	25.702699400914664	26.161041246054424	24.86035869733856
78-79	23.16314848549976	25.717609983735613	26.244358338098607	24.87488319266602
80-81	23.265558961317904	25.814330769938998	26.02879635161536	24.891313917127743
82-83	23.229187904812353	25.813420279992517	26.088700237240236	24.868691577954895
84-85	23.15665835863065	25.747122690883106	26.082809454463234	25.01340949602301
86-87	23.1117703966029	25.788059539998837	26.038651872058296	25.06151819133997
88-89	23.00595175450044	25.62324190021697	26.05236769365093	25.31843865163166
90-91	22.93684133595449	25.649179081178495	26.08869540441059	25.325284178456425
92-93	23.068612968003148	25.4364344895545	26.171488239845537	25.323464302596822
94-95	22.90406678696057	25.28453941237335	26.263295885055175	25.548097915610906
96-97	22.881530686919636	25.1289403765996	26.274248632847463	25.715280303633303
98-99	22.689186670673905	25.1628052230649	26.218785101806784	25.929223004454414
100-101	22.694167381082146	25.129241201776114	26.177072955000387	25.999518462141353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	29.0
2	46.0
3	74.5
4	72.5
5	79.5
6	136.5
7	263.5
8	372.5
9	382.0
10	338.0
11	303.0
12	295.5
13	326.5
14	375.0
15	427.0
16	469.0
17	511.5
18	594.5
19	680.0
20	778.5
21	937.5
22	1140.0
23	1432.5
24	1802.5
25	2322.0
26	3142.0
27	4403.0
28	6310.0
29	9147.5
30	13169.5
31	18591.0
32	26482.0
33	32671.0
34	37593.0
35	44391.5
36	52879.5
37	61690.0
38	69149.5
39	76626.5
40	84134.5
41	91289.0
42	97344.5
43	103465.0
44	109866.0
45	116730.5
46	122108.0
47	125868.5
48	129005.5
49	130531.5
50	130720.5
51	130170.5
52	128881.5
53	127186.0
54	123869.5
55	116950.0
56	107863.0
57	96846.5
58	86247.5
59	75075.0
60	61935.0
61	50770.5
62	41449.0
63	31569.0
64	22367.5
65	16091.0
66	12084.5
67	9362.0
68	7305.0
69	5528.5
70	4170.5
71	3041.0
72	2174.0
73	1648.5
74	1261.5
75	931.5
76	636.5
77	390.0
78	232.0
79	144.5
80	91.0
81	57.5
82	34.5
83	20.5
84	16.0
85	11.5
86	8.0
87	6.0
88	5.0
89	3.0
90	3.5
91	2.5
92	2.5
93	2.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15305759236847724
2	1.34319958199629E-4
3	0.01051053672912097
4	0.006514517972682007
5	0.02384179258043415
6	0.002149119331194064
7	0.0
8	0.0
9	0.0
10-11	0.04533298589237479
12-13	3.1900990072411886E-4
14-15	0.004785148510861783
16-17	0.0
18-19	0.0015446795192957336
20-21	2.3505992684935074E-4
22-23	1.6789994774953625E-5
24-25	6.044398118983306E-4
26-27	0.002602449190117812
28-29	0.0053895883227601145
30-31	0.016454194879454553
32-33	0.011501146420843234
34-35	0.010157946838846943
36-37	0.0035762688870651224
38-39	0.0
40-41	0.0037273788400397053
42-43	0.008898697230725422
44-45	0.006598467946556775
46-47	0.0037777488243645654
48-49	0.009654246995598335
50-51	0.0027199791535424873
52-53	0.0019980093782194816
54-55	0.003962438766889056
56-57	0.0075890776382790395
58-59	0.01472482541763433
60-61	0.0073372277166547355
62-63	0.021239343390316336
64-65	0.040379937433763474
66-67	0.05960448145108537
68-69	0.049916654465937134
70-71	0.06731108905278908
72-73	0.03282443978503434
74-75	0.02676325167127608
76-77	0.22166151101893775
78-79	0.08208628445474828
80-81	0.06588393949691804
82-83	0.027300531504074597
84-85	0.05132701402703324
86-87	0.0676636789430631
88-89	0.29118887938202076
90-91	0.29520168813323466
92-93	0.050873684168109494
94-95	0.008630057314326164
96-97	0.014271495558710582
98-99	6.380198014482377E-4
100-101	1.6789994774953627E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2977964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.18043252417267
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02857735485348	52.81477718807732
2	11.06307453793091	14.42191966858997
3	3.4143047814351264	6.676375872698771
4	1.47802035408405	3.853520238349169
5	0.7823389812869841	2.5496596590403127
6	0.5176415536914555	2.024406021725629
7	0.3353195903048643	1.52993931509296
8	0.24491093286262774	1.2770720427107751
9	0.18391779728203836	1.0789057418162729
>10	0.9032698425168973	10.193422379878669
>50	0.037337755103711075	1.624332434715012
>100	0.009943852410572421	1.126021871021764
>500	8.262561463539914E-4	0.4002154157078657
>1k	5.164100909971943E-4	0.4294321505755457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	3.357998954990725E-5
14-15	0.0	0.0	0.0	3.357998954990725E-5	1.34319958199629E-4
16-17	0.0	0.0	0.0	3.357998954990725E-5	2.3505992684935077E-4
18-19	0.0	0.0	0.0	3.357998954990725E-5	2.3505992684935077E-4
20-21	0.0	0.0	0.0	3.357998954990725E-5	2.68639916399258E-4
22-23	0.0	0.0	0.0	5.0369984324860876E-5	3.0221990594916524E-4
24-25	0.0	0.0	0.0	2.014799372994435E-4	3.3579989549907253E-4
26-27	0.0	0.0	0.0	5.876498171233769E-4	3.6937988504897977E-4
28-29	0.0	0.0	0.0	0.0057085982234842325	3.6937988504897977E-4
30-31	0.0	0.0	0.0	0.02325414276331077	3.6937988504897977E-4
32-33	0.0	0.0	0.0	0.06187113074570411	3.6937988504897977E-4
34-35	0.0	0.0	0.0	0.1108979154885687	4.02959874598887E-4
36-37	0.0	1.6789994774953625E-5	0.0	0.1878632515369561	4.02959874598887E-4
38-39	0.0	3.357998954990725E-5	0.0	0.3100272535195187	4.365398641487943E-4
40-41	0.0	3.357998954990725E-5	0.0	0.4462444811287175	4.7011985369870154E-4
42-43	0.0	3.357998954990725E-5	0.0	0.5918808958066653	4.7011985369870154E-4
44-45	0.0	3.357998954990725E-5	0.0	0.7644316721088636	4.7011985369870154E-4
46-47	0.0	3.357998954990725E-5	0.0	0.9668686391104795	4.8690984847365516E-4
48-49	0.0	3.357998954990725E-5	0.0	1.1728818749991605	5.036998432486088E-4
50-51	0.0	3.357998954990725E-5	0.0	1.3914708169742818	5.036998432486088E-4
52-53	0.0	3.357998954990725E-5	0.0	1.6143076276274662	6.044398118983305E-4
54-55	0.0	3.357998954990725E-5	0.0	1.8711777576894817	6.715997909981451E-4
56-57	0.0	3.357998954990725E-5	0.0	2.171617924192502	6.715997909981451E-4
58-59	0.0	3.357998954990725E-5	0.0	2.482132087560494	6.715997909981451E-4
60-61	0.0	3.357998954990725E-5	0.0	2.8091676057870414	7.219697753230059E-4
62-63	0.0	5.0369984324860876E-5	0.0	3.1436075117093427	7.387597700979595E-4
64-65	0.0	1.0073996864972175E-4	0.0	3.5082861982213354	7.387597700979595E-4
66-67	0.0	1.0073996864972175E-4	0.0	3.9318809763986406	7.555497648729132E-4
68-69	0.0	1.0073996864972175E-4	0.0	4.380711116722701	7.723397596478668E-4
70-71	0.0	1.0073996864972175E-4	0.0	4.8467174217015385	7.723397596478668E-4
72-73	0.0	1.0073996864972175E-4	0.0	5.327985831930809	8.562897335226349E-4
74-75	0.0	1.0073996864972175E-4	0.0	5.828344466219202	9.234497126224495E-4
76-77	0.0	1.0073996864972175E-4	0.0	6.387518452204258	9.402397073974031E-4
78-79	0.0	1.0073996864972175E-4	0.0	7.004769030115877	9.402397073974031E-4
80-81	0.0	1.1752996342467538E-4	0.0	7.639968112441924	9.402397073974031E-4
82-83	0.0	1.34319958199629E-4	0.0	8.29638974816351	9.402397073974031E-4
84-85	0.0	1.34319958199629E-4	0.0	8.973714927379916	9.738196969473103E-4
86-87	0.0	1.34319958199629E-4	0.0	9.701057501030906	9.90609691722264E-4
88-89	0.0	1.34319958199629E-4	0.0	10.475848599915917	0.0010409796760471248
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3085	0.0	40.683865	1
GGTATCA	1885	0.0	39.84916	1
TCAACGC	3670	0.0	32.852016	4
CAACGCA	3810	0.0	31.665602	5
ATCAACG	4100	0.0	29.985933	3
AACGCAG	4150	0.0	29.063496	6
TATCAAC	4265	0.0	28.38068	2
GTACATG	5805	0.0	25.060667	1
ACGCAGA	5060	0.0	23.365078	7
TACATGG	6065	0.0	23.088348	2
CGCAGAG	5420	0.0	21.725552	8
ACATGGG	6590	0.0	21.104927	3
CATGGGG	5365	0.0	19.110779	4
AGAGTAC	5215	0.0	18.02724	10-11
GCAGAGT	6565	0.0	17.213161	9
GAGTACA	4695	0.0	17.112936	1
GTATAGA	800	0.0	16.639545	1
TAAGGTG	1430	0.0	16.275677	5
GTAAGGT	1150	0.0	16.0976	4
GTATATA	860	0.0	16.031456	1
>>END_MODULE