FastQCFastQC Report
Mon 15 Aug 2016
SRR2921657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969309
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29940.10083154026744943No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG29860.100562117314163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22500.050.4139251
GTATCAA40650.040.8641661
TCAACGC48600.033.011064
CAACGCA48600.033.009395
ATCAACG50800.031.486953
GTACATG70050.030.8275071
AACGCAG53050.030.5818426
TATCAAC52300.030.4946732
TACATGG74200.028.403082
ACATGGG77350.026.2632143
ACGCAGA62950.025.1719917
CATGGGG63600.023.7328054
CGCAGAG66400.023.7232078
GCAGAGT77200.019.2988039
CGTATAC2552.6997805E-718.6133373
GAGTACA52550.018.6049981
GTGTAAG12550.018.5305141
AGAGTAC65250.017.7796710-11
ATGGGGG36400.017.4727215
CAGAGTA70250.017.32472610-11
Produced by FastQC (version 0.11.5)