##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921657_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2969309
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.612396688926616	31.0	31.0	34.0	27.0	34.0
2	30.659495525726694	33.0	31.0	34.0	27.0	34.0
3	30.717103541598398	33.0	31.0	34.0	27.0	34.0
4	34.18624703592654	37.0	35.0	37.0	30.0	37.0
5	34.193708367839115	37.0	35.0	37.0	30.0	37.0
6	34.24530286339347	37.0	35.0	37.0	30.0	37.0
7	34.152363394985166	37.0	35.0	37.0	30.0	37.0
8	34.12329299510425	37.0	35.0	37.0	30.0	37.0
9	35.710080695542295	39.0	37.0	39.0	30.0	39.0
10-11	35.61641125931993	39.0	37.0	39.0	30.0	39.0
12-13	35.516235090386346	39.0	37.0	39.0	30.0	39.0
14-15	36.68458368596869	40.0	37.0	41.0	30.0	41.0
16-17	36.62584695631206	40.0	37.0	41.0	30.0	41.0
18-19	36.59804132880748	40.0	37.0	41.0	30.0	41.0
20-21	36.520649922254634	40.0	37.0	41.0	29.0	41.0
22-23	36.422328898743785	40.0	37.0	41.0	29.0	41.0
24-25	36.31414379574507	40.0	37.0	41.0	28.5	41.0
26-27	36.16603896731529	40.0	36.0	41.0	27.5	41.0
28-29	36.02869960654145	40.0	36.0	41.0	27.0	41.0
30-31	35.87008021058098	40.0	36.0	41.0	27.0	41.0
32-33	35.68012322058769	40.0	36.0	41.0	26.5	41.0
34-35	35.507176753918166	39.5	35.0	41.0	25.5	41.0
36-37	35.4600231905807	39.5	35.0	41.0	25.5	41.0
38-39	35.62063126471513	40.0	35.5	41.0	26.0	41.0
40-41	35.66370946910544	40.0	36.0	41.0	26.0	41.0
42-43	35.484845127266986	40.0	35.0	41.0	25.0	41.0
44-45	35.35077807665016	40.0	35.0	41.0	24.5	41.0
46-47	35.17502893770907	40.0	35.0	41.0	24.0	41.0
48-49	34.99845216513337	39.5	35.0	41.0	23.0	41.0
50-51	33.27534958470136	37.0	32.5	39.5	19.0	40.0
52-53	33.39230373127216	37.5	33.5	39.5	20.5	40.0
54-55	34.294867256994806	39.0	34.0	40.5	22.0	41.0
56-57	34.306889919506524	39.0	34.0	41.0	21.0	41.0
58-59	34.109148626835406	38.0	34.0	41.0	20.0	41.0
60-61	33.85435382440831	38.0	34.0	40.0	19.5	41.0
62-63	33.553062177092386	37.0	34.0	40.0	18.0	41.0
64-65	33.250788146333036	37.0	33.5	40.0	16.5	41.0
66-67	32.9017229261084	36.0	33.0	39.5	13.0	41.0
68-69	32.53753011222476	36.0	33.0	39.0	9.5	41.0
70-71	32.118766857878384	35.0	33.0	39.0	7.0	40.5
72-73	31.750519733715826	35.0	32.0	37.5	3.5	40.0
74-75	31.35490513112647	35.0	32.0	37.0	2.0	39.0
76-77	30.971844122656147	35.0	32.0	36.5	2.0	39.0
78-79	30.612400730270913	35.0	31.5	36.0	2.0	38.5
80-81	30.287051465509315	35.0	31.0	36.0	2.0	37.0
82-83	29.95517189352809	35.0	31.0	35.0	2.0	37.0
84-85	29.679309900047453	35.0	31.0	35.0	2.0	36.5
86-87	29.423775026445547	35.0	31.0	35.0	2.0	36.0
88-89	29.159206064441257	35.0	30.5	35.0	2.0	36.0
90-91	28.90400274946124	35.0	30.0	35.0	2.0	35.5
92-93	28.688187217968895	34.0	30.0	35.0	2.0	35.0
94-95	28.423786982089098	34.0	30.0	35.0	2.0	35.0
96-97	28.147932060960983	34.0	29.0	35.0	2.0	35.0
98-99	27.831075681244357	34.0	29.0	35.0	2.0	35.0
100-101	26.71661942222921	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88169.0
3	15800.0
4	11017.0
5	8598.0
6	7954.0
7	8246.0
8	8874.0
9	9292.0
10	10422.0
11	11002.0
12	11280.0
13	11839.0
14	12617.0
15	13972.0
16	14201.0
17	14849.0
18	13600.0
19	13366.0
20	12940.0
21	13810.0
22	14767.0
23	17191.0
24	19874.0
25	23113.0
26	27480.0
27	32430.0
28	38368.0
29	45138.0
30	53676.0
31	65269.0
32	80874.0
33	103374.0
34	142878.0
35	209999.0
36	353593.0
37	637637.0
38	667737.0
39	124042.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03565898786769	17.61390574166146	11.849735232225683	25.50070003824517
2	16.701835485422382	19.604012918937812	37.89210199665197	25.802049598987843
3	19.168067252379423	22.82399195743073	29.77762619510375	28.230314595086092
4	13.364722804413667	15.017169171185179	36.0307585811312	35.58734944326995
5	14.080848479701608	36.85576963905508	34.69905081186152	14.364331069381791
6	34.450303538180066	34.88809639930697	16.41733333692603	14.244266725586934
7	29.272954844463968	31.608639446204545	20.820379575693845	18.29802613363764
8	27.099888977814423	34.01927258910743	20.01710469230417	18.863733740773974
9	26.83188711552633	14.64593597303447	19.159657865417547	39.36251904602165
10-11	25.754293485354363	24.657114828365845	28.20130436064014	21.387287325639655
12-13	26.213039527525765	22.402305442498257	27.62603256892284	23.75862246105313
14-15	23.225451273663726	24.16436059576058	24.678684458915495	27.931503671660202
16-17	23.50975524346547	27.204456960387645	25.876471144075225	23.409316652071656
18-19	23.598974060158607	26.341611695579463	27.15961852282851	22.89979572143342
20-21	23.921581573128254	25.745718318792843	27.064004706493446	23.26869540158546
22-23	23.826436578883666	25.678401669175788	26.790315182259516	23.70484656968103
24-25	23.63898346797131	25.84692051948804	26.796834585550467	23.71726142699018
26-27	23.41058853093296	25.984388168882376	26.777898072050355	23.827125228134307
28-29	23.603680920843225	26.292695923212765	26.57062424151993	23.53299891442408
30-31	23.74869313451014	25.972570644837074	26.397167200640233	23.88156902001256
32-33	23.407308809863906	26.084076809831576	26.326023131327997	24.18259124897652
34-35	24.010204274088053	26.08019202815084	26.332469785945328	23.577133911815782
36-37	24.09647906997186	25.744130786975255	26.369752464102064	23.789637678950818
38-39	23.75270948768623	25.88842232038572	26.44808560970714	23.91078258222091
40-41	23.95185078350079	25.784995265670563	26.58626978790575	23.6768841629229
42-43	23.81339204565113	25.795439106083016	26.31443214144965	24.07673670681619
44-45	23.682005078364714	25.827393979561347	26.647342851709826	23.843258090364117
46-47	23.941384017535185	25.923001770054853	26.22046885361528	23.915145358794675
48-49	23.55912059287739	26.050671732298653	26.220939876782563	24.169267798041393
50-51	23.674700533354436	26.07836424249041	26.363492632102204	23.883442592052955
52-53	24.042126763346523	25.740551962455736	26.366385257961543	23.850936016236197
54-55	23.288140472418302	25.768350913084554	26.716137119865895	24.227371494631242
56-57	23.434414341291625	25.988891523184282	26.53522001382021	24.041474121703885
58-59	23.481440441717368	25.664494003053527	26.585481062758383	24.26858449247072
60-61	23.22705685133877	25.690801070860008	26.864153243084647	24.217988834716575
62-63	23.446885062938954	25.674153103732465	26.83963369742087	24.039328135907713
64-65	23.17239466100046	25.702039223243368	26.646770598480053	24.478795517276115
66-67	23.135618459762803	25.767819522990333	26.58966521241349	24.506896804833374
68-69	23.52525202246499	25.48242613120101	26.65022091248626	24.34210093384774
70-71	23.34326738526575	25.467345853478136	26.5715137011632	24.617873060092915
72-73	23.36746205866442	25.670484507759028	26.483779490569802	24.47827394300675
74-75	23.325507948364947	25.894723627459932	26.379078566403873	24.40068985777125
76-77	23.495369296319648	25.53343232909607	26.52797361432787	24.443224760256413
78-79	23.425487852046956	25.665668540740345	26.46757249519556	24.441271112017134
80-81	23.603471322099807	25.730466218405702	26.341086762799744	24.32497569669475
82-83	23.55492878163149	25.80063177326371	26.36121789089262	24.283221554212183
84-85	23.497841546930744	25.80315043017177	26.44317648686871	24.255831536028786
86-87	23.647340389887887	25.899644876689216	26.19920157598523	24.253813157437666
88-89	23.54371738215719	25.71564945889888	26.167491130174657	24.573142028769272
90-91	23.503763452022213	25.64400155895512	26.308903682871126	24.543331306151543
92-93	23.738319870764744	25.53989662597205	26.417882157388924	24.30390134587428
94-95	23.674464633513786	25.3400079973088	26.50636940578713	24.479157963390286
96-97	23.816538682018074	25.083252477824658	26.433438192293647	24.666770647863625
98-99	23.73962444788627	25.187998860326537	26.34873737953422	24.723639312252978
100-101	23.953258722843977	25.051484613696594	26.450093685116727	24.545162978342702
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	74.5
2	117.0
3	172.5
4	151.0
5	157.5
6	281.0
7	513.5
8	773.5
9	885.0
10	792.0
11	719.0
12	751.5
13	848.0
14	985.0
15	1083.0
16	1172.0
17	1319.5
18	1457.0
19	1629.0
20	1796.0
21	1995.5
22	2229.5
23	2592.0
24	3087.5
25	3724.5
26	4783.0
27	6360.0
28	8716.5
29	11853.5
30	16097.5
31	22211.0
32	31918.0
33	36815.0
34	37762.0
35	43018.0
36	50115.0
37	57766.5
38	65154.5
39	72058.0
40	78530.0
41	84609.0
42	90846.0
43	97680.0
44	104105.0
45	112831.5
46	118873.5
47	121882.5
48	125535.5
49	126547.5
50	125959.0
51	125664.0
52	125446.0
53	125327.0
54	124017.0
55	117873.5
56	108479.5
57	97951.5
58	89760.5
59	79331.5
60	64361.5
61	52684.5
62	44267.0
63	34374.5
64	24330.5
65	17684.0
66	13349.5
67	10394.0
68	8052.0
69	6187.0
70	4735.5
71	3482.5
72	2524.0
73	1904.5
74	1493.5
75	1133.0
76	820.0
77	557.0
78	364.0
79	254.0
80	207.5
81	151.0
82	120.0
83	105.5
84	87.0
85	71.0
86	54.0
87	46.0
88	41.0
89	34.5
90	43.0
91	39.5
92	31.0
93	26.0
94	15.5
95	15.0
96	15.0
97	8.0
98	3.5
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054356080825538874
2	0.07382188920048402
3	0.07065616949936837
4	0.06978054490118744
5	0.06503196534951397
6	0.010945307477261546
7	0.013976315701733973
8	0.01795030426270893
9	0.00623040579474888
10-11	0.014195221851279203
12-13	0.022951467833088438
14-15	0.04167636308649588
16-17	0.02790211459972674
18-19	0.010911629608100739
20-21	0.02170538667413866
22-23	0.0383085761704154
24-25	0.03504182286181735
26-27	0.02232842725361355
28-29	0.012730234542784196
30-31	0.012561845196980174
32-33	0.008823601720130846
34-35	0.005422136934889565
36-37	7.57752056118107E-4
38-39	0.003805599215170937
40-41	0.0013302758318517877
42-43	0.23040714186364572
44-45	0.20050119404885108
46-47	0.20624327074076831
48-49	0.2133661400682785
50-51	0.2048456391705949
52-53	0.21215373677848956
54-55	0.20858388264744424
56-57	0.21070558840457493
58-59	0.21922608930225854
60-61	0.22373892376980636
62-63	0.21013306462884127
64-65	0.21030145397464528
66-67	0.2075061908342985
68-69	0.20312806784339388
70-71	0.1941529157120394
72-73	0.2059738477874819
74-75	0.20508138425472056
76-77	0.19811006533843398
78-79	0.21631295361984895
80-81	0.22729193896627126
82-83	0.22549017296616825
84-85	0.2202195864425023
86-87	0.23906235423797254
88-89	0.2316195451534347
90-91	0.2380520181631484
92-93	0.23438113042462067
94-95	0.23784995094818356
96-97	0.24365938337842238
98-99	0.23766472266779914
100-101	0.24446765223828168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2969309.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.09763463077662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.31310814073316	45.498063435608124
2	11.989848979131436	13.931637305355366
3	3.9024098242744243	6.801623404507459
4	1.7852988022697536	4.14886550084125
5	1.0362995807458208	3.0103277205098844
6	0.6233547256730855	2.1729261058513747
7	0.4390394212846018	1.7855006320410285
8	0.3196813340987731	1.48581834773998
9	0.24013714945344744	1.255626033320033
>10	1.264390558073815	12.935612912067013
>50	0.061162229825397665	2.390028085830819
>100	0.021603990652270474	2.1570607458467563
>500	0.0021526152383497944	0.8371708082019621
>1k	0.001512648545662554	1.5897389622790044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA	2994	0.10083154026744943	No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG	2986	0.100562117314163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.367786916080475E-5	0.0	0.0	0.0
6	0.0	3.367786916080475E-5	0.0	0.0	6.73557383216095E-5
7	0.0	3.367786916080475E-5	0.0	0.0	6.73557383216095E-5
8	0.0	3.367786916080475E-5	0.0	0.0	1.34711476643219E-4
9	0.0	3.367786916080475E-5	0.0	0.0	1.34711476643219E-4
10-11	0.0	3.367786916080475E-5	0.0	0.0	1.34711476643219E-4
12-13	0.0	6.73557383216095E-5	0.0	1.6838934580402377E-5	2.3574508412563326E-4
14-15	0.0	1.1787254206281663E-4	0.0	3.367786916080475E-5	3.7045656076885227E-4
16-17	0.0	1.34711476643219E-4	0.0	6.73557383216095E-5	4.714901682512665E-4
18-19	0.0	1.34711476643219E-4	0.0	1.0103360748241426E-4	5.051680374120713E-4
20-21	0.0	1.34711476643219E-4	0.0	1.0103360748241426E-4	5.38845906572876E-4
22-23	1.6838934580402377E-5	1.34711476643219E-4	0.0	1.34711476643219E-4	6.062016448944855E-4
24-25	3.367786916080475E-5	1.34711476643219E-4	0.0	4.5465123367086416E-4	6.73557383216095E-4
26-27	3.367786916080475E-5	1.34711476643219E-4	0.0	0.001296597962690983	6.73557383216095E-4
28-29	3.367786916080475E-5	1.34711476643219E-4	0.0	0.008756245981809236	6.73557383216095E-4
30-31	3.367786916080475E-5	1.34711476643219E-4	0.0	0.03697830033856362	6.73557383216095E-4
32-33	3.367786916080475E-5	1.34711476643219E-4	0.0	0.0935571205287156	6.73557383216095E-4
34-35	3.367786916080475E-5	1.34711476643219E-4	0.0	0.17076363557986052	6.73557383216095E-4
36-37	3.367786916080475E-5	1.34711476643219E-4	0.0	0.28981490306330526	6.73557383216095E-4
38-39	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	0.47381394122336207	7.072352523768998E-4
40-41	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	0.6731027319824242	7.072352523768998E-4
42-43	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	0.8796322646110593	7.577520561181069E-4
44-45	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	1.1391539243642208	8.251077944397164E-4
46-47	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	1.4423052636152047	8.419467290201188E-4
48-49	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	1.7437390315389876	8.419467290201188E-4
50-51	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	2.0567074696503465	9.093024673417283E-4
52-53	3.367786916080475E-5	1.34711476643219E-4	3.367786916080475E-5	2.36905286718223	9.093024673417283E-4
54-55	3.367786916080475E-5	1.5155041122362137E-4	3.367786916080475E-5	2.7298943963056725	9.766582056633377E-4
56-57	3.367786916080475E-5	1.6838934580402375E-4	3.367786916080475E-5	3.14494045584343	9.9349714024374E-4
58-59	3.367786916080475E-5	2.0206721496482852E-4	3.367786916080475E-5	3.5651392293627913	0.0010103360748241427
60-61	3.367786916080475E-5	2.0206721496482852E-4	3.367786916080475E-5	3.9964348607706373	0.001077691813145752
62-63	3.367786916080475E-5	2.0206721496482852E-4	3.367786916080475E-5	4.434449227076064	0.0011450475514673615
64-65	3.367786916080475E-5	2.0206721496482852E-4	3.367786916080475E-5	4.914207312206308	0.001161886486047764
66-67	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	5.471794952967172	0.0011787254206281665
68-69	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	6.059136991131607	0.0012124032897889712
70-71	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	6.65728962529666	0.0012292422243693735
72-73	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	7.263339719779921	0.001481826243075409
74-75	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	7.896803599760078	0.0015491819813970185
76-77	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	8.613990662473995	0.0015828598505578234
78-79	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	9.38630839700415	0.0015828598505578234
80-81	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	10.169824023030273	0.0015996987851382258
82-83	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	10.980096716104656	0.0016502155888794329
84-85	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	11.81055929174094	0.0016502155888794329
86-87	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	12.714304910671135	0.0016502155888794329
88-89	3.367786916080475E-5	2.69422953286438E-4	3.367786916080475E-5	13.668853595230406	0.0016502155888794329
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2250	0.0	50.413925	1
GTATCAA	4065	0.0	40.864166	1
TCAACGC	4860	0.0	33.01106	4
CAACGCA	4860	0.0	33.00939	5
ATCAACG	5080	0.0	31.48695	3
GTACATG	7005	0.0	30.827507	1
AACGCAG	5305	0.0	30.581842	6
TATCAAC	5230	0.0	30.494673	2
TACATGG	7420	0.0	28.40308	2
ACATGGG	7735	0.0	26.263214	3
ACGCAGA	6295	0.0	25.171991	7
CATGGGG	6360	0.0	23.732805	4
CGCAGAG	6640	0.0	23.723207	8
GCAGAGT	7720	0.0	19.298803	9
CGTATAC	255	2.6997805E-7	18.613337	3
GAGTACA	5255	0.0	18.604998	1
GTGTAAG	1255	0.0	18.530514	1
AGAGTAC	6525	0.0	17.77967	10-11
ATGGGGG	3640	0.0	17.472721	5
CAGAGTA	7025	0.0	17.324726	10-11
>>END_MODULE