FastQCFastQC Report
Mon 15 Aug 2016
SRR2921657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969309
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30150.10153877551982633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.048.602051
GTATCAA38800.040.303791
TCAACGC48800.031.7028754
CAACGCA50850.030.5407475
ATCAACG51400.030.007913
TATCAAC51900.029.6273732
AACGCAG53800.028.7725036
GTACATG69750.028.6211321
TACATGG74500.026.500412
ACATGGG78350.024.107913
ACGCAGA64150.023.907877
CGCAGAG67850.022.3241988
CATGGGG64300.021.8464894
GAGTACA54450.020.9505331
AGAGTAC63850.018.88958710-11
GCAGAGT77150.018.5874869
TAAGGTG15350.018.5637515
ATGGGGG35350.018.1370745
GGTAAGG12300.017.3629263
TATACTG11750.016.9759585
Produced by FastQC (version 0.11.5)