##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2969309
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39849709141083	33.0	31.0	34.0	29.0	34.0
2	31.524101735454277	34.0	31.0	34.0	28.0	34.0
3	31.597714821865964	34.0	31.0	34.0	28.0	34.0
4	35.072869142281924	37.0	35.0	37.0	32.0	37.0
5	34.809212176974505	37.0	35.0	37.0	32.0	37.0
6	34.78349036762425	37.0	35.0	37.0	32.0	37.0
7	34.66424780984397	37.0	35.0	37.0	32.0	37.0
8	34.60567088167651	37.0	35.0	37.0	32.0	37.0
9	36.20725529070905	39.0	37.0	39.0	32.0	39.0
10-11	36.09960684455542	39.0	37.0	39.0	32.0	39.0
12-13	36.02327982705741	39.0	37.0	39.0	31.0	39.0
14-15	37.27042992157435	40.0	38.0	41.0	31.0	41.0
16-17	37.23224005989272	40.0	38.0	41.0	31.0	41.0
18-19	37.1909360056498	40.0	38.0	41.0	31.0	41.0
20-21	37.13101617244955	40.0	38.0	41.0	31.0	41.0
22-23	37.038053634700866	40.0	38.0	41.0	31.0	41.0
24-25	36.946544297006476	40.0	37.0	41.0	30.5	41.0
26-27	36.81389744213216	40.0	37.0	41.0	30.0	41.0
28-29	36.68961398089589	40.0	37.0	41.0	30.0	41.0
30-31	36.524194854762506	40.0	37.0	41.0	30.0	41.0
32-33	36.45467160878171	40.0	37.0	41.0	30.0	41.0
34-35	36.569147400960965	40.0	37.0	41.0	30.0	41.0
36-37	36.54718892510008	40.0	37.0	41.0	30.0	41.0
38-39	36.42410692184613	40.0	37.0	41.0	30.0	41.0
40-41	36.32717005875778	40.0	37.0	41.0	29.0	41.0
42-43	36.18564234978576	40.0	36.0	41.0	28.5	41.0
44-45	36.039997016140795	40.0	36.0	41.0	28.0	41.0
46-47	35.86306460526674	40.0	36.0	41.0	27.5	41.0
48-49	35.70151220368106	40.0	35.0	41.0	27.0	41.0
50-51	35.52948160666337	39.5	35.0	41.0	26.5	41.0
52-53	35.33570588308593	39.0	35.0	41.0	26.0	41.0
54-55	35.110491195089494	39.0	35.0	41.0	26.0	41.0
56-57	34.87634092645797	39.0	35.0	41.0	25.5	41.0
58-59	34.623590875856976	38.0	35.0	40.5	25.0	41.0
60-61	34.336052765138284	38.0	34.0	40.0	24.0	41.0
62-63	34.02123372811654	37.0	34.0	40.0	23.0	41.0
64-65	33.67734041825893	37.0	34.0	40.0	22.5	41.0
66-67	33.32195302004608	36.0	34.0	39.0	22.0	41.0
68-69	32.95712504155007	36.0	33.0	39.0	21.5	41.0
70-71	32.55190854168428	35.0	33.0	38.5	20.0	40.0
72-73	32.19089441348139	35.0	33.0	37.0	19.5	39.5
74-75	31.793904069936808	35.0	33.0	37.0	17.5	39.0
76-77	29.10216939361986	33.0	28.5	34.5	9.0	36.5
78-79	30.662706205383138	34.5	31.5	35.5	10.5	37.0
80-81	30.848081152887758	35.0	32.0	35.5	10.5	37.0
82-83	30.67382815328415	35.0	32.0	35.0	7.0	37.0
84-85	30.476055876973398	35.0	32.0	35.0	4.5	36.0
86-87	30.26885177662547	35.0	32.0	35.0	2.0	36.0
88-89	29.991811226113548	35.0	31.0	35.0	2.0	36.0
90-91	29.792869990964228	34.5	31.0	35.0	2.0	35.0
92-93	29.632836629667036	34.0	31.0	35.0	2.0	35.0
94-95	29.52007049451573	34.0	31.0	35.0	2.0	35.0
96-97	29.311100158319665	34.0	31.0	35.0	2.0	35.0
98-99	29.096215314741578	34.0	31.0	35.0	2.0	35.0
100-101	27.952342110571855	33.0	28.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56003.0
3	19668.0
4	10905.0
5	7619.0
6	6672.0
7	6675.0
8	7292.0
9	7792.0
10	8496.0
11	9430.0
12	9787.0
13	10962.0
14	11583.0
15	12638.0
16	13725.0
17	14807.0
18	13751.0
19	12648.0
20	11984.0
21	11803.0
22	12390.0
23	13579.0
24	15917.0
25	19093.0
26	22454.0
27	26859.0
28	32148.0
29	38783.0
30	47197.0
31	57998.0
32	73730.0
33	97141.0
34	137518.0
35	208768.0
36	370256.0
37	706820.0
38	729144.0
39	95269.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.27248469670361	18.863922946814764	12.32299080351667	26.540601552964954
2	15.874600874346607	20.6859320183895	36.44528699159365	26.99418011567024
3	18.02445209463558	23.786682941566976	29.409775516177394	28.77908944762005
4	13.061293499362101	15.994313474277261	35.00857491974064	35.93581810662
5	13.938164889137422	37.2182958626126	34.60677639513027	14.236762853119716
6	34.59883936903868	35.30659456757892	16.098896454218863	13.995669609163535
7	29.176249423687462	31.751663434152526	20.753448024439354	18.318639117720657
8	27.15402809205778	34.4045702215566	19.69441375080869	18.746987935576932
9	26.744581565805902	14.61727783039011	18.94619891233917	39.69194169146481
10-11	25.73347599449639	24.88980739226749	28.090434508367363	21.28628210486876
12-13	26.131997170375428	22.525067789580604	27.54943633993012	23.79349870011385
14-15	23.150222874858333	24.267897768893842	24.602235316108455	27.979644040139366
16-17	23.51810194191678	27.38019643294053	25.717267168433324	23.384434456709364
18-19	23.597470885853415	26.515267603622583	27.000927166725997	22.886334343798
20-21	23.96167516810348	25.813625559138142	26.927793131180323	23.296906141578056
22-23	23.78922166739804	25.772006214240417	26.696952051807337	23.741820066554205
24-25	23.595873427760992	26.00653185902029	26.692974841649992	23.70461987156873
26-27	23.405662666569444	26.09689179260745	26.723015930617457	23.774429610205644
28-29	23.592853798924267	26.404754928211293	26.492474226456906	23.509917046407534
30-31	23.72023101761061	26.08487397845174	26.331776912925115	23.86311809101253
32-33	23.453071979432284	26.232866480774437	26.183926199537915	24.130135340255368
34-35	23.98187393397876	26.21888497883451	26.28171792101271	23.51752316617402
36-37	24.051808916940033	25.86043958264571	26.32848126418742	23.759270236226836
38-39	23.764097977004077	25.98707308670132	26.385212855920347	23.863616080374257
40-41	23.900131180336757	25.878192574081528	26.52518611947009	23.696490126111623
42-43	23.795133244999565	25.913257542331834	26.215493078238705	24.076116134429892
44-45	23.6663989074075	25.9353639252497	26.51485221878121	23.883384948561595
46-47	23.856256972656663	26.061338329088347	26.144862862315026	23.937541835939964
48-49	23.50657195741418	26.169894980374814	26.14414571289853	24.179387349312474
50-51	23.681874272535243	26.16295008514032	26.209410160796654	23.945765481527783
52-53	23.973020032062074	25.864783580983687	26.218106316767926	23.94409007018631
54-55	23.21119714480621	25.92786809715998	26.527190706003378	24.333744052030433
56-57	23.345903989243823	26.06637265904232	26.311650327171616	24.27607302454225
58-59	23.282532290489698	25.84985631736255	26.393143922290957	24.47446746985679
60-61	23.088809590768935	25.967437998992963	26.573058211144435	24.370694199093666
62-63	23.324302998200388	25.877847102980343	26.49798438540808	24.299865513411184
64-65	23.002026518246243	25.816781188250577	26.35166071738409	24.82953157611909
66-67	22.985490768675536	25.978152414155876	26.265257068182972	24.771099748985613
68-69	23.32072717666792	25.687969993213972	26.259660995019313	24.731641835098802
70-71	23.083808311239967	25.575373964010083	26.219993657600043	25.120824067149904
72-73	23.01853230604414	25.770702718363523	26.142106841220485	25.06865813437185
74-75	22.962986917942423	25.927415626211143	26.018672869060005	25.09092458678643
76-77	23.01920915701911	25.535678090719255	26.254888614769982	25.19022413749165
78-79	22.902424740356864	25.621639385704352	26.203374834102256	25.27256103983653
80-81	22.994220975850833	25.68099456437764	26.008422611339775	25.31636184843175
82-83	22.851274581405313	25.681488049199192	26.103737774986996	25.363499594408495
84-85	22.708020007267958	25.61087316066684	26.198661206544937	25.482445625520267
86-87	22.75298671927038	25.73779350527045	25.959540592607294	25.549679182851875
88-89	22.5835630592312	25.512756141631737	25.96807025348127	25.935610545655795
90-91	22.421209378553534	25.394926331366392	26.072425440053532	26.11143885002654
92-93	22.480542968891495	25.25529798172526	26.299065877181675	25.96509317220157
94-95	22.286284271470176	24.96857103064958	26.47141887839881	26.27372581948143
96-97	22.28595632308187	24.736926313094823	26.46305212970027	26.514065234123034
98-99	22.1851724685492	24.8668490565307	26.300462351064507	26.647516123855592
100-101	22.01406256419859	24.70824793166879	26.407165724469927	26.870523779662697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	60.5
2	101.0
3	155.5
4	137.0
5	150.0
6	254.5
7	428.0
8	621.5
9	715.0
10	628.5
11	504.0
12	500.5
13	562.0
14	592.0
15	646.0
16	740.5
17	803.0
18	916.0
19	1044.0
20	1132.5
21	1350.0
22	1650.0
23	1968.0
24	2410.5
25	3120.0
26	4232.5
27	5819.5
28	8260.0
29	11672.0
30	16149.0
31	22249.5
32	31960.0
33	36839.0
34	37740.5
35	42645.0
36	49488.0
37	56933.5
38	63921.5
39	70930.5
40	77280.5
41	83462.0
42	90165.5
43	96897.0
44	103066.5
45	111769.5
46	118163.0
47	121306.5
48	125384.5
49	126941.5
50	126847.5
51	127099.0
52	126922.5
53	128281.0
54	128022.0
55	121232.0
56	111395.0
57	100534.0
58	91702.5
59	80800.0
60	65926.0
61	53958.5
62	44575.0
63	33992.5
64	23868.0
65	17248.5
66	13032.0
67	10252.5
68	7845.0
69	5895.0
70	4442.0
71	3177.5
72	2250.0
73	1628.5
74	1235.0
75	929.0
76	666.0
77	418.0
78	235.5
79	132.0
80	88.0
81	52.5
82	28.5
83	20.5
84	20.0
85	14.0
86	10.5
87	7.0
88	3.0
89	4.5
90	6.0
91	5.0
92	1.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16552672692535536
2	2.0206721496482852E-4
3	0.010776918131457522
4	0.00623040579474888
5	0.025898281384658852
6	0.0020880278879698948
7	0.0
8	0.0
9	3.367786916080475E-5
10-11	0.048411936918656834
12-13	3.536176261884499E-4
14-15	0.005186391850763932
16-17	1.6838934580402377E-5
18-19	0.0016165377197186281
20-21	2.3574508412563326E-4
22-23	0.0
24-25	5.556848411532784E-4
26-27	0.003014169289892025
28-29	0.00602833857978405
30-31	0.017882948524387324
32-33	0.012949140692329426
34-35	0.010945307477261546
36-37	0.004344445121743813
38-39	0.0
40-41	0.003839277084331742
42-43	0.008991991065934868
44-45	0.007543842692020264
46-47	0.0040918611030377776
48-49	0.00985077672953539
50-51	0.0031320418319548417
52-53	0.002323772972095528
54-55	0.004159216841359387
56-57	0.007577520561181069
58-59	0.014801423496173688
60-61	0.006870285308804169
62-63	0.020594016991832106
64-65	0.03920103970317673
66-67	0.058936271031408326
68-69	0.04873187667568448
70-71	0.0666148252000718
72-73	0.03297063390842785
74-75	0.026184543272525698
76-77	0.22515339427456016
78-79	0.08407680035994906
80-81	0.06531822723738082
82-83	0.027110684674447827
84-85	0.05009583037669707
86-87	0.06616017396640093
88-89	0.2935194686709938
90-91	0.2959779531197326
92-93	0.05225121400298857
94-95	0.010524334112751485
96-97	0.015626531290613404
98-99	9.598192710829355E-4
100-101	2.3574508412563326E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2969309.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.46330546362471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87523464042187	44.74968396194009
2	12.126483979484567	13.936557062257457
3	3.991015872325087	6.880108925447733
4	1.8600511288099137	4.275387447710561
5	1.057188923004248	3.037478500767676
6	0.6721922341676823	2.317583260935232
7	0.45992654397629346	1.8500229641137278
8	0.31560093753456775	1.4508378462524163
9	0.23914326240791875	1.2367766103582594
>10	1.3158014240054723	13.335885952775229
>50	0.0625368117558974	2.427040625462655
>100	0.021178031847756486	2.1268454540501933
>500	0.0021759641868720936	0.8503266323969525
>1k	0.0014702460718297014	1.5254647555318055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA	3015	0.10153877551982633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.367786916080475E-5
2	0.0	0.0	0.0	0.0	3.367786916080475E-5
3	0.0	0.0	0.0	0.0	3.367786916080475E-5
4	0.0	0.0	0.0	3.367786916080475E-5	3.367786916080475E-5
5	0.0	3.367786916080475E-5	0.0	3.367786916080475E-5	3.367786916080475E-5
6	0.0	3.367786916080475E-5	0.0	3.367786916080475E-5	3.367786916080475E-5
7	0.0	3.367786916080475E-5	0.0	3.367786916080475E-5	3.367786916080475E-5
8	0.0	3.367786916080475E-5	0.0	3.367786916080475E-5	3.367786916080475E-5
9	0.0	3.367786916080475E-5	0.0	3.367786916080475E-5	3.367786916080475E-5
10-11	0.0	3.367786916080475E-5	0.0	3.367786916080475E-5	3.367786916080475E-5
12-13	0.0	3.367786916080475E-5	0.0	5.051680374120713E-5	1.34711476643219E-4
14-15	0.0	3.367786916080475E-5	0.0	6.73557383216095E-5	2.189061495452309E-4
16-17	0.0	3.367786916080475E-5	0.0	6.73557383216095E-5	2.5258401870603567E-4
18-19	0.0	3.367786916080475E-5	0.0	1.0103360748241426E-4	2.69422953286438E-4
20-21	0.0	3.367786916080475E-5	0.0	1.1787254206281663E-4	3.536176261884499E-4
22-23	0.0	3.367786916080475E-5	0.0	1.8522828038442614E-4	4.0413442992965704E-4
24-25	0.0	3.367786916080475E-5	0.0	5.220069719924737E-4	4.378122990904618E-4
26-27	0.0	3.367786916080475E-5	0.0	0.0013134368972713855	5.051680374120713E-4
28-29	0.0	3.367786916080475E-5	0.0	0.00857101770142481	5.051680374120713E-4
30-31	0.0	3.367786916080475E-5	0.0	0.036574165908633965	5.051680374120713E-4
32-33	0.0	3.367786916080475E-5	0.0	0.0935234426595548	5.051680374120713E-4
34-35	0.0	3.367786916080475E-5	0.0	0.17163926017804143	5.725237757336808E-4
36-37	0.0	3.367786916080475E-5	0.0	0.29084207807270984	5.725237757336808E-4
38-39	0.0	3.367786916080475E-5	0.0	0.4749084719710882	6.567184486356927E-4
40-41	0.0	3.367786916080475E-5	0.0	0.6739446787114443	6.73557383216095E-4
42-43	0.0	3.367786916080475E-5	0.0	0.880373177732597	6.73557383216095E-4
44-45	0.0	3.367786916080475E-5	0.0	1.1406357506072962	6.903963177964975E-4
46-47	0.0	3.367786916080475E-5	0.0	1.4434334722320916	7.072352523768998E-4
48-49	0.0	5.051680374120713E-5	0.0	1.74466517294091	8.082688598593141E-4
50-51	0.0	6.73557383216095E-5	0.0	2.0558823618559066	8.082688598593141E-4
52-53	0.0	6.73557383216095E-5	0.0	2.367133228640064	9.261414019221307E-4
54-55	0.0	6.73557383216095E-5	0.0	2.727149649969067	9.9349714024374E-4
56-57	0.0	6.73557383216095E-5	0.0	3.141774736142315	0.0010103360748241427
58-59	0.0	6.73557383216095E-5	0.0	3.5609800125214317	0.0010103360748241427
60-61	0.0	6.73557383216095E-5	0.0	3.9912484689198733	0.0010103360748241427
62-63	0.0	6.73557383216095E-5	0.0	4.428521922103762	0.0010440139439849474
64-65	0.0	6.73557383216095E-5	0.0	4.909088276093866	0.001077691813145752
66-67	0.0	6.73557383216095E-5	0.0	5.4660865541444155	0.001077691813145752
68-69	0.0	6.73557383216095E-5	0.0	6.0509027521217895	0.001077691813145752
70-71	0.0	8.419467290201189E-5	0.0	6.647001036268034	0.0011113696823065568
72-73	0.0	1.0103360748241426E-4	0.0	7.251468270900737	0.0013302758318517877
74-75	0.0	1.0103360748241426E-4	0.0	7.885369963179985	0.00134711476643219
76-77	0.0	1.0103360748241426E-4	0.0	8.604022013202398	0.00134711476643219
78-79	0.0	1.0103360748241426E-4	0.0	9.378983460461676	0.0013639537010125924
80-81	0.0	1.0103360748241426E-4	0.0	10.16751708899276	0.0013976315701733973
82-83	0.0	1.0103360748241426E-4	0.0	10.980652400945809	0.001481826243075409
84-85	0.0	1.0103360748241426E-4	0.0	11.814044951199083	0.0015491819813970185
86-87	0.0	1.34711476643219E-4	0.0	12.720131182035956	0.0015828598505578234
88-89	0.0	1.34711476643219E-4	0.0	13.677121512109384	0.0015828598505578234
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2220	0.0	48.60205	1
GTATCAA	3880	0.0	40.30379	1
TCAACGC	4880	0.0	31.702875	4
CAACGCA	5085	0.0	30.540747	5
ATCAACG	5140	0.0	30.00791	3
TATCAAC	5190	0.0	29.627373	2
AACGCAG	5380	0.0	28.772503	6
GTACATG	6975	0.0	28.621132	1
TACATGG	7450	0.0	26.50041	2
ACATGGG	7835	0.0	24.10791	3
ACGCAGA	6415	0.0	23.90787	7
CGCAGAG	6785	0.0	22.324198	8
CATGGGG	6430	0.0	21.846489	4
GAGTACA	5445	0.0	20.950533	1
AGAGTAC	6385	0.0	18.889587	10-11
GCAGAGT	7715	0.0	18.587486	9
TAAGGTG	1535	0.0	18.563751	5
ATGGGGG	3535	0.0	18.137074	5
GGTAAGG	1230	0.0	17.362926	3
TATACTG	1175	0.0	16.975958	5
>>END_MODULE