FastQCFastQC Report
Mon 15 Aug 2016
SRR2921656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037888
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.038.9193081
GTATCAA32850.037.9989551
TCAACGC35250.034.0698244
CAACGCA35550.033.6471255
ATCAACG36950.032.5012663
AACGCAG37900.031.6704146
TATCAAC40950.029.905622
GTACATG67050.026.4742741
ACGCAGA47300.025.0793157
TACATGG68350.024.7922952
ACATGGG70300.024.1049963
CGCAGAG51000.023.0748988
TATACCG2303.6616257E-922.7013595
CATGGGG57700.021.6365684
GAGTACA51800.020.2494751
GCAGAGT61300.018.1919789
TCTATAC8150.018.055033
CCTATAC7900.018.0255433
TAGTCCG1350.009439466517.5810454
GTATAAT10450.017.2591151
Produced by FastQC (version 0.11.5)