##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921656_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3037888
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.40999832778562	31.0	31.0	34.0	26.0	34.0
2	30.44997346840963	33.0	31.0	34.0	26.0	34.0
3	30.515366596793562	33.0	31.0	34.0	26.0	34.0
4	33.96152590220574	37.0	35.0	37.0	30.0	37.0
5	33.95941160437778	37.0	35.0	37.0	30.0	37.0
6	34.010780845113445	37.0	35.0	37.0	30.0	37.0
7	33.917578594075884	37.0	35.0	37.0	30.0	37.0
8	33.88803471359049	37.0	35.0	37.0	30.0	37.0
9	35.45798660121769	39.0	37.0	39.0	30.0	39.0
10-11	35.35910244222302	39.0	37.0	39.0	30.0	39.0
12-13	35.250532442275684	39.0	37.0	39.0	29.0	39.0
14-15	36.419736672319715	40.0	37.0	41.0	29.0	41.0
16-17	36.35448377293699	40.0	37.0	41.0	28.0	41.0
18-19	36.31387101828639	40.0	37.0	41.0	28.0	41.0
20-21	36.239339304148146	40.0	37.0	41.0	27.0	41.0
22-23	36.14126360155476	40.0	37.0	41.0	27.0	41.0
24-25	36.03797243348011	40.0	36.5	41.0	27.0	41.0
26-27	35.896417346524956	40.0	36.0	41.0	27.0	41.0
28-29	35.766812502633414	40.0	36.0	41.0	27.0	41.0
30-31	35.626817710198665	40.0	36.0	41.0	26.0	41.0
32-33	35.46594509079993	40.0	36.0	41.0	25.0	41.0
34-35	35.29987906071587	39.5	35.0	41.0	25.0	41.0
36-37	35.27347749489119	39.5	35.0	41.0	24.5	41.0
38-39	35.45479721438052	40.0	35.5	41.0	25.0	41.0
40-41	35.52106545731772	40.0	36.0	41.0	25.0	41.0
42-43	35.36400848220869	40.0	35.0	41.0	24.0	41.0
44-45	35.25157444909095	40.0	35.0	41.0	24.0	41.0
46-47	35.10522408989404	40.0	35.0	41.0	23.5	41.0
48-49	34.94527859486591	40.0	35.0	41.0	23.0	41.0
50-51	33.26298434965344	37.0	32.5	39.5	18.5	40.0
52-53	33.38359758490109	37.5	33.5	39.5	19.0	40.0
54-55	34.29067200634125	39.0	34.0	40.5	21.0	41.0
56-57	34.311024139138766	39.0	34.0	41.0	20.0	41.0
58-59	34.12258351854973	38.5	34.0	41.0	19.5	41.0
60-61	33.871686842964586	38.0	34.0	40.5	18.5	41.0
62-63	33.5803151729096	37.0	34.0	40.0	17.5	41.0
64-65	33.28829996365896	37.0	34.0	40.0	14.5	41.0
66-67	32.95038197589905	36.0	33.0	39.5	13.0	41.0
68-69	32.59121238175996	36.0	33.0	39.0	9.5	41.0
70-71	32.18831224192597	35.0	33.0	39.0	7.0	40.5
72-73	31.836134347283377	35.0	32.5	37.5	4.0	40.0
74-75	31.441791468283228	35.0	32.0	37.0	2.0	39.0
76-77	31.072181726251923	35.0	32.0	36.5	2.0	39.0
78-79	30.73273208228875	35.0	32.0	36.0	2.0	38.5
80-81	30.425041344513033	35.0	31.5	36.0	2.0	37.0
82-83	30.11659465391746	35.0	31.0	35.0	2.0	37.0
84-85	29.86024830408494	35.0	31.0	35.0	2.0	36.5
86-87	29.628630647344472	35.0	31.0	35.0	2.0	36.0
88-89	29.408359689363138	35.0	31.0	35.0	2.0	36.0
90-91	29.188366391387703	35.0	31.0	35.0	2.0	35.5
92-93	28.99962111835591	34.5	30.5	35.0	2.0	35.0
94-95	28.77260665962669	34.0	30.0	35.0	2.0	35.0
96-97	28.544099222881158	34.0	30.0	35.0	2.0	35.0
98-99	28.277737197684708	34.0	30.0	35.0	2.0	35.0
100-101	27.211469613099627	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109216.0
3	18342.0
4	12279.0
5	9193.0
6	8431.0
7	8386.0
8	8993.0
9	9400.0
10	10226.0
11	10917.0
12	10678.0
13	10805.0
14	11472.0
15	12121.0
16	11975.0
17	12409.0
18	12145.0
19	11575.0
20	11738.0
21	12315.0
22	13574.0
23	15830.0
24	18276.0
25	21440.0
26	25643.0
27	30501.0
28	36356.0
29	43330.0
30	51604.0
31	63245.0
32	79317.0
33	102076.0
34	142524.0
35	209981.0
36	361746.0
37	665350.0
38	708657.0
39	125810.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.55152442096902	17.178265070811623	11.416937220348272	25.853273287871094
2	16.58489632504518	18.889970083057232	38.32992175214066	26.195211839756922
3	19.747230131322542	22.305233006267695	28.98437489706458	28.963161965345186
4	13.180585791369099	14.442139769995906	35.8903032057813	36.4869712328537
5	14.178651703157568	36.811596016874795	34.72583076197427	14.283921517993365
6	35.31514632163864	34.630096435034496	16.075347915731854	13.979409327595011
7	30.11181238248967	30.926890840128674	20.357965549947508	18.60333122743415
8	27.480399315992592	34.59363520175519	19.233955160245923	18.6920103220063
9	26.75584612675071	14.75223812792265	18.597291696500143	39.89462404882649
10-11	26.252196706372537	24.647885005507817	27.727932360209145	21.3719859279105
12-13	26.396723183336317	21.98738910897348	27.544781141677117	24.071106566013086
14-15	23.284963908579268	23.761934768208594	24.343362377820263	28.60973894539188
16-17	23.988160384999862	26.959423685551837	25.3190387383721	23.7333771910762
18-19	24.004888748060754	26.279308826494713	26.418086721781677	23.29771570366286
20-21	24.277159853124804	25.63391990675845	26.439823888044288	23.64909635207246
22-23	24.114831590542003	25.516029234768894	26.196516393105227	24.172622781583875
24-25	23.908537292122727	25.708784782316634	26.301891936745776	24.080785988814863
26-27	23.850716297519746	25.836986445470995	26.211288795573495	24.101008461435764
28-29	24.022991835755107	25.960399181830663	26.092217547710906	23.924391434703324
30-31	24.041523409510173	25.827934046756308	25.98268222828822	24.147860315445303
32-33	23.801531195742992	25.925179729170083	25.965145149534536	24.30814392555239
34-35	24.173758751430967	25.873423055303856	26.019453751048278	23.933364442216895
36-37	24.277254383019624	25.623695627843286	26.05574318763867	24.043306801498424
38-39	24.012231346792884	25.817717841237737	26.06255216617066	24.107498645798717
40-41	24.189422751382928	25.639066175244636	26.195397041427814	23.976114031944626
42-43	24.0156548465252	25.62072525391375	26.155594778441007	24.208025121120038
44-45	23.99210686045873	25.743312688071757	26.211512329031855	24.05306812243765
46-47	24.189585073428223	25.74446103575395	26.01149225315938	24.05446163765844
48-49	23.937133391911466	25.858090511133803	25.996949715157225	24.207826381797503
50-51	23.986562530455192	25.944648931102133	26.05444015463116	24.01434838381152
52-53	24.23151755143693	25.74527930678841	26.022199816155915	24.00100332561874
54-55	23.704288818070506	25.700916470496082	26.376029122571232	24.218765588862183
56-57	23.83694860777509	25.849265854806884	26.183096396599193	24.130689140818838
58-59	23.846411383824755	25.665821148080102	26.258217418122687	24.229550049972456
60-61	23.729355039713994	25.768690680117206	26.36317185493498	24.138782425233813
62-63	23.839857519201512	25.748391113090012	26.33798787099221	24.073763496716264
64-65	23.611869003818132	25.667999623348624	26.315628904727816	24.40450246810543
66-67	23.64323898066867	25.70522912494911	26.266879482224297	24.38465241215793
68-69	23.892331016314554	25.562751281989556	26.348929375064518	24.195988326631383
70-71	23.746811131952526	25.477126549585417	26.259470971077924	24.51659134738414
72-73	23.72647110670785	25.603637615095693	26.258432078266637	24.411459199929816
74-75	23.70228360619946	25.811293300362053	26.213419327773774	24.27300376566471
76-77	23.873520693350457	25.56511323700681	26.22106951824042	24.340296551402314
78-79	23.768786092848913	25.624290017296865	26.219422092220903	24.387501797633327
80-81	23.8810160079408	25.74780640047133	26.132855319579885	24.23832227200799
82-83	23.829027896302694	25.79006866949675	26.157793709139142	24.223109725061416
84-85	23.807617729148618	25.741580125552034	26.171824169103868	24.27897797619548
86-87	23.86141862379006	25.81840729995904	26.102018567275653	24.21815550897525
88-89	23.73012613379386	25.70823457223869	26.093349234075752	24.468290059891697
90-91	23.770019382242566	25.72008742628562	26.127576394902885	24.382316796568933
92-93	23.914886962907417	25.548080249020245	26.297163256153134	24.239869531919204
94-95	23.79291278237509	25.437569425680746	26.365692010112436	24.403825781831724
96-97	23.91509299344312	25.248887349945452	26.340397094696215	24.49562256191521
98-99	23.818633286689387	25.329890599790144	26.268900407555822	24.582575705964647
100-101	24.04737242279117	25.196660617347312	26.318267443262677	24.43769951659884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	26.5
2	46.0
3	74.0
4	65.0
5	82.0
6	134.0
7	215.5
8	355.0
9	429.5
10	412.0
11	396.0
12	421.5
13	475.5
14	555.0
15	659.5
16	738.0
17	834.0
18	944.5
19	1022.5
20	1126.0
21	1275.0
22	1404.5
23	1657.0
24	1957.0
25	2347.0
26	2945.0
27	3991.5
28	5769.5
29	8261.5
30	11818.5
31	17143.5
32	25023.0
33	30512.0
34	34504.0
35	40812.0
36	49013.0
37	57593.0
38	65616.0
39	73719.5
40	81724.0
41	89241.5
42	95643.0
43	102995.0
44	110352.0
45	118513.5
46	124327.0
47	127770.0
48	131531.5
49	133412.5
50	133503.5
51	133407.0
52	132845.5
53	131455.5
54	128977.0
55	122180.5
56	112445.5
57	102202.5
58	92830.0
59	81385.0
60	67156.0
61	55726.0
62	46284.0
63	35358.5
64	25289.5
65	18634.0
66	14189.5
67	10910.5
68	8317.5
69	6380.5
70	4930.5
71	3608.5
72	2559.5
73	1851.5
74	1420.0
75	1123.0
76	828.0
77	544.0
78	353.0
79	257.5
80	178.5
81	122.5
82	114.5
83	98.0
84	79.0
85	72.5
86	64.0
87	51.5
88	37.5
89	30.5
90	34.5
91	28.0
92	18.5
93	15.5
94	14.5
95	12.0
96	10.5
97	15.5
98	13.0
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05010059620367835
2	0.07077285271873092
3	0.06596688225504034
4	0.06678982240293257
5	0.06188509912149493
6	0.00984236416879095
7	0.01280494870120294
8	0.016261297322350265
9	0.006353097941727937
10-11	0.012706195883455874
12-13	0.02212063117534287
14-15	0.040307608443760926
16-17	0.026202414308888276
18-19	0.009891740577664484
20-21	0.020260786441106453
22-23	0.03597894326584785
24-25	0.03291760591568879
26-27	0.020984973771251605
28-29	0.012344102218383298
30-31	0.012261808203594076
32-33	0.008624412749910463
34-35	0.005612451808624939
36-37	9.216929656392862E-4
38-39	0.003933653906924811
40-41	0.0012344102218383298
42-43	0.21378339161944085
44-45	0.18547425053194852
46-47	0.19241986538015884
48-49	0.19882233973076033
50-51	0.1899181273305665
52-53	0.19655102492257778
54-55	0.1934073935576295
56-57	0.19620539006046303
58-59	0.20468167358375292
60-61	0.20859886868771987
62-63	0.19423033370552173
64-65	0.19442783934101587
66-67	0.1922881949564961
68-69	0.18720242484252217
70-71	0.17986179872332356
72-73	0.19064231466071166
74-75	0.18940790443887331
76-77	0.184256299113068
78-79	0.20158741862767818
80-81	0.21248314618577116
82-83	0.2110347715254809
84-85	0.20529064929319316
86-87	0.22392201424147304
88-89	0.2166965997429793
90-91	0.22262176880780332
92-93	0.21885270293045697
94-95	0.22281927444329744
96-97	0.2287279847051636
98-99	0.2228357332462553
100-101	0.22938633682347737
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3037888.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.74580351221703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76467539411026	47.05858819800067
2	11.687792974684893	13.965931651139885
3	3.889139496768897	6.970792926166713
4	1.7656900346454012	4.219702794936152
5	1.0102842604880748	3.0180122459303007
6	0.6166298527642249	2.2104627613811223
7	0.42405827278735864	1.7734991570578562
8	0.3110040979450202	1.4864951781853981
9	0.22881879003343844	1.2303866222316953
>10	1.2223319350429902	12.759467661004456
>50	0.060251551142966056	2.438760877349171
>100	0.017444118179449034	1.716051410133168
>500	0.0011606955754445297	0.48648353983634235
>1k	7.185258316057148E-4	0.6653649766470993
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2917605915688795E-5
2	0.0	0.0	0.0	0.0	3.2917605915688795E-5
3	0.0	0.0	0.0	0.0	3.2917605915688795E-5
4	0.0	0.0	0.0	3.2917605915688795E-5	3.2917605915688795E-5
5	0.0	0.0	0.0	3.2917605915688795E-5	3.2917605915688795E-5
6	0.0	0.0	0.0	3.2917605915688795E-5	9.875281774706638E-5
7	0.0	0.0	0.0	3.2917605915688795E-5	9.875281774706638E-5
8	0.0	0.0	0.0	3.2917605915688795E-5	1.3167042366275518E-4
9	0.0	0.0	0.0	6.583521183137759E-5	1.3167042366275518E-4
10-11	0.0	0.0	0.0	6.583521183137759E-5	1.3167042366275518E-4
12-13	0.0	0.0	0.0	6.583521183137759E-5	1.9750563549413277E-4
14-15	0.0	0.0	0.0	6.583521183137759E-5	2.7979965028335473E-4
16-17	0.0	0.0	0.0	6.583521183137759E-5	3.9501127098826554E-4
18-19	0.0	0.0	0.0	6.583521183137759E-5	4.608464828196431E-4
20-21	0.0	0.0	0.0	1.1521162070491077E-4	4.7730528577748753E-4
22-23	1.6458802957844397E-5	0.0	0.0	1.3167042366275518E-4	6.254345123980871E-4
24-25	3.2917605915688795E-5	0.0	0.0	2.797996502833548E-4	7.241873301451535E-4
26-27	3.2917605915688795E-5	0.0	0.0	6.58352118313776E-4	7.241873301451535E-4
28-29	3.2917605915688795E-5	0.0	0.0	0.004526170813407209	7.241873301451535E-4
30-31	3.2917605915688795E-5	0.0	0.0	0.01818697726841806	7.241873301451535E-4
32-33	3.2917605915688795E-5	0.0	0.0	0.045344002148861313	7.241873301451535E-4
34-35	4.937640887353319E-5	0.0	0.0	0.07872245454736976	7.571049360608423E-4
36-37	6.583521183137759E-5	1.6458802957844397E-5	0.0	0.1358345008110898	7.735637390186866E-4
38-39	6.583521183137759E-5	4.937640887353319E-5	0.0	0.23228308614405796	8.229401478922198E-4
40-41	6.583521183137759E-5	6.583521183137759E-5	0.0	0.3358254155518571	8.229401478922198E-4
42-43	6.583521183137759E-5	9.875281774706638E-5	0.0	0.4448649851475762	8.558577538079087E-4
44-45	6.583521183137759E-5	9.875281774706638E-5	0.0	0.5744451408346851	9.216929656392862E-4
46-47	6.583521183137759E-5	9.875281774706638E-5	0.0	0.7296845703330735	0.0010039869804285082
48-49	6.583521183137759E-5	9.875281774706638E-5	0.0	0.8918366970737566	0.0011521162070491078
50-51	6.583521183137759E-5	9.875281774706638E-5	0.0	1.0696082278214338	0.0011685750100069521
52-53	6.583521183137759E-5	9.875281774706638E-5	0.0	1.2451578201698021	0.0012837866307118628
54-55	6.583521183137759E-5	9.875281774706638E-5	0.0	1.4466464859797332	0.0013496218425432405
56-57	6.583521183137759E-5	9.875281774706638E-5	0.0	1.675374470684897	0.0013496218425432405
58-59	6.583521183137759E-5	9.875281774706638E-5	0.0	1.9158046642930877	0.0013989982514167737
60-61	9.875281774706638E-5	9.875281774706638E-5	0.0	2.1774173373080243	0.001448374660290307
62-63	9.875281774706638E-5	9.875281774706638E-5	0.0	2.4445107917079234	0.0015471274780373733
64-65	9.875281774706638E-5	9.875281774706638E-5	0.0	2.7310256335980787	0.0015635862809952176
66-67	9.875281774706638E-5	9.875281774706638E-5	0.0	3.058654565276929	0.001612962689868751
68-69	9.875281774706638E-5	9.875281774706638E-5	0.0	3.420764689152464	0.0016458802957844396
70-71	1.1521162070491077E-4	9.875281774706638E-5	0.0	3.8014897191733206	0.0018104683253628837
72-73	1.3167042366275518E-4	9.875281774706638E-5	0.0	4.199858585964986	0.0020408915667727056
74-75	1.3167042366275518E-4	9.875281774706638E-5	0.0	4.598424958392146	0.002073809172688394
76-77	1.3167042366275518E-4	9.875281774706638E-5	0.0	5.037907914972507	0.002106726778604083
78-79	1.3167042366275518E-4	9.875281774706638E-5	0.0	5.5323303558261525	0.0021561031874776163
80-81	1.3167042366275518E-4	1.1521162070491077E-4	0.0	6.050930778224872	0.0022383972022668383
82-83	1.3167042366275518E-4	1.3167042366275518E-4	0.0	6.585660827522279	0.0024359028377609706
84-85	1.3167042366275518E-4	1.3167042366275518E-4	0.0	7.133113531506099	0.0024359028377609706
86-87	1.3167042366275518E-4	1.3167042366275518E-4	0.0	7.722766606273832	0.0024688204436766597
88-89	1.3167042366275518E-4	1.3167042366275518E-4	0.0	8.36232277160975	0.0025017380495923483
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2000	0.0	38.919308	1
GTATCAA	3285	0.0	37.998955	1
TCAACGC	3525	0.0	34.069824	4
CAACGCA	3555	0.0	33.647125	5
ATCAACG	3695	0.0	32.501266	3
AACGCAG	3790	0.0	31.670414	6
TATCAAC	4095	0.0	29.90562	2
GTACATG	6705	0.0	26.474274	1
ACGCAGA	4730	0.0	25.079315	7
TACATGG	6835	0.0	24.792295	2
ACATGGG	7030	0.0	24.104996	3
CGCAGAG	5100	0.0	23.074898	8
TATACCG	230	3.6616257E-9	22.701359	5
CATGGGG	5770	0.0	21.636568	4
GAGTACA	5180	0.0	20.249475	1
GCAGAGT	6130	0.0	18.191978	9
TCTATAC	815	0.0	18.05503	3
CCTATAC	790	0.0	18.025543	3
TAGTCCG	135	0.0094394665	17.581045	4
GTATAAT	1045	0.0	17.259115	1
>>END_MODULE