FastQCFastQC Report
Mon 15 Aug 2016
SRR2921656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037888
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.040.3596531
GTATCAA28650.034.6719741
GTACATG64500.028.6646581
TCAACGC33650.028.3490314
CAACGCA34150.027.8137655
ATCAACG35600.026.7966463
TACATGG70550.026.2362632
ACATGGG69600.025.8442753
AACGCAG37250.025.751936
TATCAAC38100.025.0383362
CATGGGG56350.023.6668034
ACGCAGA46700.019.9307617
GTATAGG6700.019.15341
CGCAGAG49600.018.5739698
TATACTG13100.018.4892525
GAGTACA46850.017.449211
GTATAGC8800.016.74311
AGAGTAC48350.016.15675410-11
TTATACT12250.015.8845544
ATGGGGG34500.015.4176935
Produced by FastQC (version 0.11.5)